=========================================
Fzds required for Phosphorylation of Lrp6
=========================================

Fzd2--

  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: Lcells in BMS
    *** reqs: Fzd2 and/or Fzd7 [RNAi]
    *** source: 18077588-Fig-1b
  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (30 min)
    *** cells: HEK293T in BMS
    *** enhanced by: xFzd2 [addition]
    *** source: 18772438-Fig-S5d
  *** Lrp6[phosAb] phos(S1490)[phosAb] is detectable
    *** cells: HEK293T in BMS "treated with Wnt3a for 30 min"
    *** partially reqs: Fzd2 [RNAi]
    *** source: 18772438-Fig-S5c

not Fzd3--

  *** Lrp6[phosAb] phos(S1490)[phosAb] is detectable
    *** cells: HEK293T in BMS "treated with Wnt3a for 30 min"
    *** does not req: Fzd3 [RNAi]
    *** source: 18772438-Fig-S5c

Fzd4--

  *** Lrp6[phosAb] phos(S1490)[phosAb] is detectable
    *** cells: HEK293T in BMS "treated with Wnt3a for 30 min"
    *** partially reqs: Fzd4 [RNAi]
    *** source: 18772438-Fig-S5c

Fzd5--

  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: Lcells<Fzd2~null><Fzd7~null><xFzd5> in BMS
    *** reqs: xFzd5 [omission]
    *** source: 18077588-Fig-1c
  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (20 min)
    *** cells: HEK293T in BMS
    *** enhanced by: xFzd5 [addition]
    *** source: 18772438-Fig-3f
  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (30 min)
    *** cells: HEK293T in BMS
    *** enhanced by: xFzd5 [addition]
    *** source: 18772438-Fig-S5d
  *** Lrp6[phosAb] phos(S1490)[phosAb] is detectable
    *** cells: HEK293T in BMS "treated with Wnt3a for 30 min"
    *** reqs: Fzd5 [RNAi]
    *** source: 18772438-Fig-S5c

not Fzd6--

  *** Lrp6[phosAb] phos(S1490)[phosAb] is detectable
    *** cells: HEK293T in BMS "treated with Wnt3a for 30 min"
    *** does not req: Fzd6 [RNAi]
    *** source: 18772438-Fig-S5c

Fzd2/Fzd4/Fzd5

  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (30 min)
    *** cells: HEK293T in BMS
    *** reqs: Fzd2 and/or Fzd4 and/or Fzd5 [RNAi]
    *** source: 18772438-Fig-3d
  *** Lrp6[phosAb] phos(S1490)[phosAb] is detectable
    *** cells: HEK293T in BMS "treated with Wnt3a for 30 min"
    *** reqs: Dvl1 and/or Dvl2 and/or Dvl3 [RNAi]
    *** source: 18772438-Fig-S6a

==============================================
Lrp6 phos requires either Dvl1, Dvl2, or Dvl3
==============================================

not Dvl1--

  *** Lrp6[phosAb] phos(S1490)[phosAb] is detectable
    *** cells: HEK293T in BMS "treated with Wnt3a for 30 min"
    *** does not req: Dvl1 [RNAi]
    *** source: 18772438-Fig-S6a

not Dvl2--

  *** Lrp6[phosAb] phos(S1490)[phosAb] is detectable
    *** cells: HEK293T in BMS "treated with Wnt3a for 30 min"
    *** does not req: Dvl2 [RNAi]
    *** source: 18772438-Fig-S6a
  *** Lrp6[phosAb] phos(S1490)[phosAb] is detectable
    *** cells: HEK293T in BMS "treated with Wnt3a for 30 min"
    *** does not req: Dvl2 [RNAi]
    *** source: 18772438-Fig-S6a

Dvl3--

  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: mEFs<Dvl1~null><Dvl2~null> in BMS
    *** partially reqs: Dvl3 [RNAi]
    *** source: 18077588-Fig-3a

Dvl1/Dvl2/Dvl3--

  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (30 min)
    *** cells: HEK293T in BMS
    *** reqs: Dvl1 and/or Dvl2 and/or Dvl3 [RNAi]
    *** source: 18772438-Fig-3i
  *** Lrp6[phosAb] phos(S1490)[phosAb] is detectable
    *** cells: HEK293T in BMS "treated with Wnt3a for 30 min"
    *** reqs: Dvl1 and/or Dvl2 and/or Dvl3 [RNAi]
    *** source: 18772438-Fig-S6a

========================================
Lrp6 phos requires either Gsk3a or Gsk3b
========================================

  *** xLrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: mEFs<xLrp6> in BMS
    *** reqs: Gsk3a and/or Gsk3b [DKO]
    *** source: 16341017-Fig-1d
  *** xLrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: mEFs<xLrp6> in BMS
    *** reqs: Gsk3a and/or Gsk3b [DKO]
    *** source: 16341017-Fig-2h
  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: mESCs in BMS
    *** reqs: Gsk3a and/or Gsk3b [KO]
    *** source: 18077588-Fig-5d
  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: mESCs<Gsk3a~null><Gsk3b~null><xGsk3a> in BMS
    *** reqs: xGsk3a [omission]
    *** source: 18077588-Fig-5d
  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: mESCs<Gsk3a~null><Gsk3b~null><xGsk3b> in BMS
    *** reqs: xGsk3b [omission]
    *** source: 18077588-Fig-5d
  *** xLrp6[phosAb] phos(S1493)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: mEFs<xLrp6> in BMS
    *** reqs: Gsk3a and/or Gsk3b [DKO]
    *** source: 16341017-Fig-2h


======================
Requirement for Csnk1s
======================

not Csnk1a1--

  *** xLrp6[phosAb] phos(S1493)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: mEFs<Csnk1e~null> in BMS
    *** unaffected by: xCsnk1a1(mnr)"DN" [addition]
    *** source: 16341017-Fig-3a
  *** xLrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: mEFs<Csnk1e~null> in BMS
    *** unaffected by: xCsnk1a1(mnr)"DN" [addition]
    *** source: 16341017-Fig-3a

not Csnk1e--

  *** xLrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: mEFs<Csnk1e~null> in BMS
    *** unaffected by: xCsnk1d(mnr)"DN" [addition]
    *** source: 16341017-Fig-3a

Csnk1g--

  *** xLrp6[phosAb] phos(T1479)[phosAb] is increased irt Wnt3a (2 hr)
    *** cells: HEK293T<xLrp6> in BMS
    *** inhibited by: xCsnk1g1(K73R) [addition]
    *** source: 16341016-Fig-4c

  *** xLrp6[phosAb] phos(S1490)[phosAb] is detectable-but unchanged itpo xCsnk1g1
    *** cells: HEK293T<xLrp6> in BMS
    *** source: 16341016-Fig-4c

=====================
Requirement for Axins
=====================

Axin1--

  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: mESCs in BMS
    *** partially reqs: Axin1 [KO]
    *** source: 18077588-Fig-5a

Axin2--

  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: mESCs<Axin1~null> in BMS
    *** partially reqs: Axin2 [RNAi]
    *** source: 18077588-Fig-5b

==================================================
Lipid Kinases required for Phosphorylation of Lrp6
==================================================

Pi4k2a--

  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (30 min)
    *** cells: HEK293T in BMS
    *** reqs: Pi4k2a [RNAi]
    *** comment: requirement for Pi4k2a can be overridden by pretreatment with PI45P2
    *** source: 18772438-Fig-2b
  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (30 min)
    *** cells: HEK293T in BMS
    *** reqs: Pi4k2a [RNAi]
    *** source: 18772438-Fig-1a

Pip5k1b--

  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (30 min)
    *** cells: HEK293T in BMS
    *** reqs: Pip5k1b [RNAi]
    *** comment: requirement for Pip5k1b can be overridden by pretreatment with PI45P2
    *** source: 18772438-Fig-2c
  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (30 min)
    *** cells: HEK293T in BMS
    *** reqs: Pip5k1b [RNAi]
    *** source: 18772438-Fig-1b

not Pip5k1a--

  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (30 min)
    *** cells: HEK293T in BMS
    *** does not req: Pip5k1a [RNAi]
    *** source: 18772438-Fig-1b

not Pip5k1c--

  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (30 min)
    *** cells: HEK293T in BMS
    *** does not req: Pip5k1c [RNAi]
    *** source: 18772438-Fig-1b

Effect of lipids

  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (30 min)
    *** cells: HEK293T in BMS
    *** unaffected by: PtdIns [pretreatment]
    *** unaffected by: PI3P [pretreatment]
    *** unaffected by: PI4P [pretreatment]
    *** unaffected by: PI5P [pretreatment]
    *** unaffected by: PI35P2 [pretreatment]
    *** unaffected by: PI34P2 [pretreatment]
    *** enhanced by: PI45P2 [pretreatment]
    *** unaffected by: PIP3 [pretreatment]
    *** source: 18772438-Fig-2a

===============================
Inhibition by Wnt5a cotreatment
===============================

Lrp6 phos(T1479)

  *** Lrp6[phosAb] phos(T1479)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: C3H10T12 in BMS
    *** inhibited by: Wnt5a [addition]
    *** source: 19910923-Fig-5c
  *** Lrp6[phosAb] phos(T1479)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: HELAS3 in BMS
    *** inhibited by: Wnt5a [addition]
    *** source: 19910923-Fig-5b
  *** Lrp6[phosAb] phos(T1479)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: Lcells in BMS
    *** inhibited by: Wnt5a [addition]
    *** source: 19910923-Fig-5c
  *** Lrp6[phosAb] phos(T1479)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: NIH3T3 in BMS
    *** inhibited by: Wnt5a [addition]
    *** source: 19910923-Fig-5c

Lrp6 phos(S1490)

  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: C3H10T12 in BMS
    *** inhibited by: Wnt5a [addition]
    *** source: 19910923-Fig-5c
  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: HELAS3 in BMS
    *** inhibited by: Wnt5a [addition]
    *** source: 19910923-Fig-5b
  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: HELAS3 in BMS
    *** partially inhibited by: Wnt5a [cotreatment]
    *** unaffected by: Clathrin [RNAi]
    *** source: 19910923-Fig-6e
  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: HELAS3 in BMS
    *** partially inhibited by: Wnt5a [cotreatment]
    *** unaffected by: Monodansylcadaverine [pretreatment]
    *** unaffected by: Chlorpromazine [pretreatment]
    *** source: 19910923-Fig-S8
  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: Lcells in BMS
    *** inhibited by: Wnt5a [addition]
    *** source: 19910923-Fig-5c
  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: mEFs in BMS
    *** repressed by: Wnt5a [KO]
    *** source: 19910923-Fig-5e
  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: NIH3T3 in BMS
    *** inhibited by: Wnt5a [addition]
    *** source: 19910923-Fig-5c

================
Other Inhibitors
================

  *** xLrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: HEK293T<xLrp6> in BMS
    *** inhibited by: xShisa2 [addition]
    *** source: 18077588-Fig-1a
  *** xLrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (1 hr)
    *** cells: mEFs<xLrp6> in BMS
    *** partially inhibited by: LiCl [addition]
    *** inhibited by: SB216763 [addition]
    *** source: 16341017-Fig-1c

=========
No extras
=========

  *** Lrp6[phosAb] phos(T1479)[phosAb] is increased irt Wnt3a (30 min)
    *** cells: HEK293T in BMS
    *** source: 18772438-Fig-4c
  *** xLrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (times)
    *** cells: HEK293T<xLrp6><xAxin1><xMesdc2> in BMS
    *** times: 0 15+ 30++ min
    *** source: 14731402-Fig-4b
  *** xLrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (times)
    *** cells: mEFs<xLrp6> in BMS
    *** times: 0 5+ 15++ 30++ 60++ 120++ min
    *** source: 16341017-Fig-2g
  *** Lrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (30 min)
    *** cells: HEK293T in BMS
    *** source: 18772438-Fig-1b
  *** Lrp6[phosAb] phos(S1490)[phosAb] is detectable
    *** cells: HEK293T in BMS "treated with Wnt3a for 30 min"
    *** source: 18772438-Fig-S5c
  *** xLrp6[phosAb] phos(S1490)[phosAb] is increased irt Wnt3a (times)
    *** cells: mEFs in BMS
    *** times: 0+ 10++ 30++ 60++ min
    *** source: 16341016-Fig-4d
  *** xLrp6[phosAb] phos(T1479)[phosAb] is increased irt Wnt3a (times)
    *** cells: mEFs in BMS
    *** times: 0 10+ 30+ 60+ min
    *** source: 16341016-Fig-4d

