=================
Kinase Activation
=================

not Msk2--

  *** Msk1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt UV (15 min)
    *** cells: mEFs in BMLS
    *** reqs: Msk1 [KO]
    *** does not req: Msk2 [KO]
    *** reqs: Msk1 and/or Msk2 [DKO]
    *** source: 11909979-Fig-4b

not Pdpk1--

  *** Msk1[Ab]IP IVKA(Creb1)[32P-ATP] is increased irt UV (15 min)
    *** cells: mESCs in BMLS
    *** does not req: Pdpk1 [KO]
    *** source: 10801415-Fig-6a

requires phos on: S376/T581/S212/S360

  *** xMsk1[tAb]IP IVKA(peptide)[32P-ATP] is increased irt UV (30 min)
    *** cells: HEK293 in BMLS
    *** partially inhibited by: xMsk1(S360A) [substitution]
    *** inhibited by: xMsk1(S376A) [substitution]
    *** inhibited by: xMsk1(T581A) [substitution]
    *** inhibited by: xMsk1(S212A) [substitution]
    *** unaffected by: xMsk1(S381A) [substitution]
    *** partially inhibited by: xMsk1(L740A)"Mapk-docking-mut" [substitution]
    *** inhibited by: xMsk1(R743A/R744A)"Mapk-docking-mut" [substitution]
    *** unaffected by: xMsk1(S750A/S752A) [substitution]
    *** unaffected by: xMsk1(S758A) [substitution]
    *** source: 15568999-Fig-3,4a,6,7

requires activity of both kinase regions

  *** xMsk1[tAb]IP IVKA(Crosstide)[32P-ATP] is increased irt UV (30 min)
    *** cells: HEK293<xMsk1> in BMLS
    *** inhibited by: xMsk1(D195A)"NT-KD" [substitution]
    *** inhibited by: xMsk1(D565A)"CT-KD" [substitution]
    *** source: 9687510-Fig-6a
  *** Msk1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt UV (30 min)
    *** cells: JB6CL41 in BMLS
    *** partially inhibited by: xMsk1(D565A)"CT-KD" [addition]
    *** source: 11350959-Fig-10a
  *** xMsk1[tAb]IP IVKA(peptide)[32P-ATP] is detectable
    *** cells: JB6CL41<xMsk1> in BMS "treated with UV for 30 min"
    *** inhibited by: xMsk1(D195A)"NT-KD" [substitution]
    *** inhibited by: xMsk1(D565A)"CT-KD" [substitution]
    *** source: 11441012-Fig-7a
  *** xMsk1[tAb]IP IVKA(peptide)[32P-ATP] is increased irt UV (30 min)
    *** cells: JB6CL41<xMsk1> in BMLS
    *** inhibited by: Msk1(mnr)"CT-KD" [substitution]
    *** inhibited by: Msk1(mnr)"NT-KD" [substitution]
    *** source: 11553624-Fig-6a

chemicals

  *** Msk1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt UV (15 min)
    *** cells: mEFs in BMLS
    *** partially inhibited by: PD184352 [addition] no-bkg-control
    *** inhibited by: SB203580 [addition] no-bkg-control
    *** source: 11909979-Fig-3b
  *** Msk1[Ab]IP IVKA(Crosstide)[32P-ATP] is increased irt UV (30 min)
    *** cells: HEK293 in BMLS
    *** unaffected by: PD98059 [pretreatment]
    *** inhibited by: SB203580 [pretreatment]
    *** source: 9687510-Fig-5a
  *** xMsk1[tAb]IP IVKA(Crosstide)[32P-ATP] is increased irt UV (30 min)
    *** cells: HEK293<xMsk1> in BMLS
    *** unaffected by: PD98059 [pretreatment]
    *** inhibited by: SB203580 [pretreatment]
    *** source: 9687510-Fig-6a
  *** Msk1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt UV (30 min)
    *** cells: JB6CL41 in BMLS
    *** partially inhibited by: PD98059 [pretreatment]
    *** inhibited by: SB202190 [pretreatment]
    *** partially inhibited by: LY294002 [pretreatment]
    *** source: 11350959-Fig-7b

no extras

  *** Msk1[Ab]IP IVKA(Akt1)(S473)[phosAb] is increased irt UV (30 min)
    *** cells: JB6CL41 in BMLS
    *** source: 11350959-Fig-5b
  *** Msk1[Ab]IP IVKA(Akt1)(T308)[phosAb] is increased irt UV (30 min)
    *** cells: JB6CL41 in BMLS
    *** source: 11350959-Fig-5b
  *** Msk1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt UV (times)
    *** cells: JB6CL41 in BMLS
    *** times: 0 5+ 30++ 60+ 120 min
    *** source: 11350959-Fig-7a


===============
Phosphorylation
===============

S212--

  *** Msk1[Ab]IP phos(S212)[phosAb] is increased irt UV (30 min)
    *** cells: HEK293 in BMLS
    *** source: 15568999-Fig-2b
  *** xMsk1[phosAb] phos(S212)[phosAb] is increased irt UV (30 min)
    *** cells: HEK293<xMsk1> in BMLS
    *** unaffected by: PD184352 [addition]
    *** inhibited by: SB203580 [addition]
    *** inhibited by: xMsk1(D565A)"CT-KD" [substitution]
    *** unaffected by: xMsk1(D195A)"NT-KD" [substitution]
    *** source: 15568999-Fig-2a


S360--

  *** Msk1[Ab]IP phos(S360)[phosAb] is increased irt UV (30 min)
    *** cells: HEK293 in BMLS
    *** source: 15568999-Fig-2b
  *** xMsk1[phosAb] phos(S360)[phosAb] is increased irt UV (30 min)
    *** cells: HEK293<xMsk1> in BMLS
    *** unaffected by: PD184352 [addition]
    *** inhibited by: SB203580 [addition]
    *** unaffected by: xMsk1(D565A)"CT-KD" [substitution]
    *** unaffected by: xMsk1(D195A)"NT-KD" [substitution]
    *** source: 15568999-Fig-2a

S376--

  *** Msk1[Ab]IP phos(S376)[phosAb] is increased irt UV (30 min)
    *** cells: HEK293 in BMLS
    *** source: 15568999-Fig-2b
  *** xMsk1[phosAb] phos(S376)[phosAb] is increased irt UV (30 min)
    *** cells: HEK293<xMsk1> in BMLS
    *** unaffected by: PD184352 [addition]
    *** inhibited by: SB203580 [addition]
    *** inhibited by: xMsk1(D565A)"CT-KD" [substitution]
    *** unaffected by: xMsk1(D195A)"NT-KD" [substitution]
    *** source: 15568999-Fig-2a
  *** Msk1[phosAb] phos(S376)[phosAb] is increased irt UV (30 min)
    *** cells: JB6CL41 in BMLS
    *** inhibited by: PD98059 [pretreatment]
    *** source: 11553624-Fig-7a
  *** Msk1[phosAb] phos(S376)[phosAb] is increased irt UV (30 min)
    *** cells: JB6CL41 in BMLS
    *** partially inhibited by: SB202190 [pretreatment]
    *** source: 11553624-Fig-7b
  *** Msk1[phosAb] phos(S376)[phosAb] is increased irt UV (30 min)
    *** cells: JB6CL41 in BMLS
    *** inhibited by: xErk2(mnr)"DN" [addition]
    *** source: 11553624-Fig-7c
  *** Msk1[phosAb] phos(S376)[phosAb] is increased irt UV (30 min)
    *** cells: JB6CL41 in BMLS
    *** inhibited by: xP38a(mnr)"DN" [addition]
    *** source: 11553624-Fig-7d

S381--

  *** Msk1[Ab]IP phos(S381)[phosAb] is increased irt UV (30 min)
    *** cells: HEK293 in BMLS
    *** source: 15568999-Fig-2b
  *** xMsk1[phosAb] phos(S381)[phosAb] is increased irt UV (30 min)
    *** cells: HEK293<xMsk1> in BMLS
    *** unaffected by: PD184352 [addition]
    *** inhibited by: SB203580 [addition]
    *** inhibited by: xMsk1(D565A)"CT-KD" [substitution]
    *** unaffected by: xMsk1(D195A)"NT-KD" [substitution]
    *** source: 15568999-Fig-2a

T581--

  *** Msk1[Ab]IP phos(T581)[phosAb] is increased irt UV (30 min)
    *** cells: HEK293 in BMLS
    *** source: 15568999-Fig-2b
  *** xMsk1[phosAb] phos(T581)[phosAb] is increased irt UV (30 min)
    *** cells: HEK293<xMsk1> in BMLS
    *** unaffected by: PD184352 [addition]
    *** inhibited by: SB203580 [addition]
    *** unaffected by: xMsk1(D565A)"CT-KD" [substitution]
    *** unaffected by: xMsk1(D195A)"NT-KD" [substitution]
    *** source: 15568999-Fig-2a

S750--

  *** Msk1[Ab]IP phos(S750)[phosAb] is increased irt UV (30 min)
    *** cells: HEK293 in BMLS
    *** source: 15568999-Fig-2b
  *** xMsk1[phosAb] phos(S750)[phosAb] is increased irt UV (30 min)
    *** cells: HEK293<xMsk1> in BMLS
    *** unaffected by: PD184352 [addition]
    *** inhibited by: SB203580 [addition]
    *** inhibited by: xMsk1(D565A)"CT-KD" [substitution]
    *** inhibited by: xMsk1(D195A)"NT-KD" [substitution]
    *** source: 15568999-Fig-2a

