=====================================
Decrease in Nfkb1 protein expression
=====================================

requires proteasomal degradation

  *** xNfkb1(436-968)"delN"[tAb] prot-exp[WB] is decreased irt Tnf (15 min)
    *** cells: HELA<xNfkb1(436-968)> in BMS
    *** reversed by: ALLN [pretreatment]
    *** source: 10469655-Fig-9b

requires S921/S923/S932 phos site

  *** xNfkb1(436-968)"delN"[tAb] prot-exp[WB] is decreased irt Tnf (15 min)
    *** cells: HELA<xNfkb1(436-968)> in BMS
    *** reversed by: xNfkb1(436-968/S921A/S923A/S932A)"delN" [substitution]
    *** source: 10469655-Fig-9b

=====================================
Decrease in Nfkb1 protein stability
=====================================

not Ikk1--

  *** Nfkb1[Ab]IP prot-stability[35S-pulse-chase] is decreased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0+ 2 4 hr
    *** does not req: Ikk1 [KO]
    *** source: 11297557-Fig-1a

Ikk2--

  *** Nfkb1[Ab]IP prot-stability[35S-pulse-chase] is decreased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0+ 2 4 hr
    *** partially reqs: Ikk2 [KO]
    *** source: 11297557-Fig-1b

Nemo--

  *** Nfkb1[Ab]IP prot-stability[35S-pulse-chase] is decreased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0+ 2 4 hr
    *** reqs: Nemo [KO]
    *** source: 11297557-Fig-1c

Tpl2-- kinase activity

  *** xNfkb1[tAb]IP prot-stability[35S-pulse-chase] is decreased irt Tnf (2 hr)
    *** cells: JURKATT<xNfkb1> in BMS
    *** inhibited by: xTpl2(D270A)"KD" [addition]
    *** source: 9950430-Fig-7a

requires proteasomal degradation

  *** xNfkb1[tAb]IP prot-stability[35S-pulse-chase] is decreased irt Tnf (45 min)
    *** cells: HELA<xNfkb1> in BMS
    *** reqs: Fbxw11 and/or Btrc [RNAi]
    *** source: 12482991-Fig-6c

requires S927 and S932 phos sites

  *** xNfkb1[tAb]IP prot-stability[35S-pulse-chase] is decreased irt Tnf (45 min)
    *** cells: HELA<xNfkb1> in BMS
    *** unaffected by: xNfkb1(S923A) [substitution]
    *** inhibited by: xNfkb1(S927A) [substitution]
    *** inhibited by: xNfkb1(S932A) [substitution]
    *** source: 12482991-Fig-2a
  *** xNfkb1[Ab]IP prot-stability[35S-pulse-chase] is decreased irt Tnf (times)
    *** cells: HELA<xNfkb1> in BMS
    *** times: 0+ 30 60 min
    *** reversed by: xNfkb1(S927A) [substitution]
    *** source: 11297557-Fig-4a
  *** xNfkb1[tAb]IP prot-stability[35S-pulse-chase] is decreased irt Tnf (20 min)
    *** cells: HELA<xNfkb1> in BMS
    *** reversed by: xNfkb1(1-902)"delC3" [substitution]
    *** reversed by: xNfkb1(1-850)"delC5" [substitution]
    *** source: 10469655-Fig-7b

no extras

  *** Nfkb1[Ab]IP prot-stability[35S-pulse-chase] is decreased irt Tnf (20 min)
    *** cells: HELA in BMS
    *** source: 10469655-Fig-7a
  *** Nfkb1{p50}[Ab]IP prot-stability[35S-pulse-chase] is unchanged irt Tnf (20 min)
    *** cells: HELA in BMS
    *** source: 10469655-Fig-7a

=================
Ubiquitination???
=================

  *** xNfkb1[tAb]IP ubiq[WBMS] is increased irt Tnf (15 min)
    *** cells: HELA<xNfkb1> in BMS "pretreated with ALLN"
    *** inhibited by: xNfkb1(S927A) [substitution]
    *** inhibited by: xNfkb1(S932A) [substitution]
    *** source: 12482991-Fig-5b
  *** xNfkb1[tAb]IP ubiq[WBMS] is increased irt Tnf (15 min)
    *** cells: HELA<xNfkb1> in BMS "pretreated with ALLN"
    *** reqs: Fbxw11 and/or Btrc [RNAi]
    *** source: 12482991-Fig-6d
  *** xNfkb1[tAb]IP ubiq[WBMS] is increased irt Tnf (15 min)
    *** cells: HELA<xNfkb1> in BMS "pretreated with ALLN"
    *** source: 12482991-Fig-5a
