==================================================
Ikk1/Ikk2/Nemo IP kinase activity irt Tnf requires
==================================================

not Azi2--

  *** Ikk2[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: HELA in BMS
    *** times: 0 10+ 30+ min
    *** does not req: Azi2 [RNAi]
    *** source: 14560022-Fig-7c

Ciap1-- and/or Ciap2--

  *** Ikk2[phosAb] phos(sitenr)[phosAb] is increased irt Tnf (times)
    *** cells: C2C12 in BMS
    *** times: 0 5++ 15+ min
    *** does not req: Ciap2 [RNAi]
    *** partially reqs: Ciap1 [RNAi]
    *** reqs: Ciap1 and/or Ciap2 [RNAi]
    *** source: 18697935-Fig-S3b
  *** Ikk2[phosAb] phos(sitenr)[phosAb] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 5++ 15+ min
    *** does not req: Ciap1 [RNAi]
    *** does not req: Ciap2 [RNAi]
    *** reqs: Ciap1 and/or Ciap2 [RNAi]
    *** source: 18697935-Fig-3a
  *** Ikk2[phosAb] phos(sitenr)[phosAb] is increased irt Tnf (times)
    *** cells: mEFs<Ciap1~null> in BMS
    *** times: 0 5++ 15+ min
    *** reqs: Ciap2 [RNAi]
    *** source: 18697935-Fig-S3a
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: HEK293T in BMS
    *** partially reqs: Ciap1 [RNAi]
    *** source: 12867425-Fig-4i
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: HEK293T in BMS
    *** partially reqs: Ciap1 [RNAi]
    *** source: 12867425-Fig-4g

inhibited by Cezanne--

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: HEK293 in BMS
    *** times: 0 5+ 15++ 30+ 60+ min
    *** partially inhibited by: xCezanne [addition]
    *** source: 18178551-Fig-4b

Erc1-- ???

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (7 min)
    *** cells: HELA in BMS
    *** reqs: Erc1 [RNAi]
    *** source: 15218148-Fig-2c
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (30 min)
    *** cells: mEFs in BMS
    *** does not req: Erc1 [KO]
    *** source: 20932476-Fig-3f

Ikk1-- or Ikk1 kinase activity

  *** xIkk1[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (tnr)
    *** cells: HELA in BMS
    *** unaffected by: xIkk1(mnr)"KD" [substitution]
    *** source: 11864612-Fig-1b
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (tnr)
    *** cells: COS in BMS
    *** inhibited by: xIkk1(S176A/S180A) [addition]
    *** inhibited by: xIkk1(K44M) [addition]
    *** source: 10779355-Fig-5a
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (20 min)
    *** cells: mEFs in BMS
    *** does not req: Ikk1 [KO]
    *** source: 11733537-Fig-4b
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (15 min)
    *** cells: mEFs<Ikk1~null><xIkk1> in BMS
    *** unaffected by: xIkk1(1-737)"delNBD" [substitution]
    *** source: 19666475-Fig-7a


  *** Nemo[Ab]IP IVKA(Rela)[32P-ATP] is increased irt Tnf (20 min)
    *** cells: mEFs in BMS
    *** reqs: Ikk1 [KO]
    *** source: 11733537-Fig-4b

  *** Ikks[phosAb] phos(CTSFV)[phosAb] is increased irt Tnf (30 min)
    *** cells: mEFs in BMS "nuclear extracts"
    *** reqs: Ikk1 [KO]
    *** source: 19666475-Fig-5g
  *** Ikks[phosAb] phos(CTSFV)[phosAb] is increased irt Tnf (30 min)
    *** cells: mEFs<Ikk1~null><xIkk1> in BMS "nuclear extracts"
    *** reqs: xIkk1 [omission]
    *** unaffected by: xIkk1(1-737)"delNBD" [substitution]
    *** source: 19666475-Fig-5g

Ikk2-- but not Ikk2 kinase activity

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (20 min)
    *** cells: mEFs in BMS
    *** partially reqs: Ikk2 [KO]
    *** source: 11733537-Fig-4b
  *** Nemo[Ab]IP IVKA(Rela)[32P-ATP] is increased irt Tnf (20 min)
    *** cells: mEFs in BMS
    *** reqs: Ikk2 [KO]
    *** source: 11733537-Fig-4b
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (7 min)
    *** cells: mEFs in BMS
    *** reqs: Ikk1 and/or Ikk2 [DKO]
    *** source: 10898787-Fig-2c
  *** Nemo[Ab]IP IVKA(Ikbb)[32P-ATP] is increased irt Tnf (7 min)
    *** cells: mEFs in BMS
    *** reqs: Ikk1 and/or Ikk2 [DKO]
    *** source: 10898787-Fig-2c
  *** Nemo[Ab]IP IVKA(Rela)[32P-ATP] is increased irt Tnf (7 min)
    *** cells: mEFs in BMS
    *** reqs: Ikk1 and/or Ikk2 [DKO]
    *** source: 10898787-Fig-2c
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (tnr)
    *** cells: HELA in BMS
    *** bkg inhibited by: xIkk2(mnr)"KD" [substitution]
    *** source: 11864612-Fig-1b
  *** xIkk1[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (tnr)
    *** cells: COS in BMS
    *** unaffected by: xIkk2(K44M) [addition]
    *** source: 10779355-Fig-5b

not Mavs--

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: HEK293<xRig1> in BMS
    *** does not req: Mavs [RNAi]
    *** source: 16125763-Fig-3e

not Mekk1--

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: mESCs in BMS
    *** times: 0 10+ 30++ 60+ min
    *** unaffected by: kMekk1(1-1188)"del-kinase-domain" [addition]
    *** source: 10805784-Fig-3a
  *** xIkk1[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (tnr)
    *** cells: COS in BMS
    *** unaffected by: xMekk1(D1369A) [addition]
    *** source: 10779355-Fig-5b
  *** xIkk1[tAb]IP phos[32Pi] is increased irt Tnf (5 min)
    *** cells: COS in BMS
    *** unaffected by: xMekk1(D1369A) [addition]
    *** source: 10779355-Fig-2a
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (tnr)
    *** cells: COS in BMS
    *** partially inhibited by: xMekk1(D1369A) [addition]
    *** source: 10779355-Fig-5a
  *** xIkk2[tAb]IP phos[32Pi] is increased irt Tnf (5 min)
    *** cells: COS in BMS
    *** unaffected by: xMekk1(D1369A) [addition]
    *** source: 10779355-Fig-2c
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: HELA in BMS
    *** partially inhibited by: xMekk1(mnr)"DN" [addition]
    *** source: 9819420-Fig-3c

Mekk3-- is required at 5-10 min but not at 30 min

  *** xIkk2[tAb]IP IVKA(auto)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Mekk3 [KO]
    *** source: 14636585-Fig-5a
  *** xIkk2[tAb]IP IVKA(auto)[32P-ATP] is increased irt Tnf (30 min)
    *** cells: mEFs in BMS "on gelatin"
    *** does not req: Mekk3 [KO]
    *** source: 14636585-Fig-5b
  *** Ikk2[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: mEFs in BMS
    *** partially reqs: Mekk3 [KO]
    *** source: 11429546-Fig-3a

Muc1--

  *** Ikk2[phosAb] phos(S181)[phosAb] is increased irt Tnf (30 min)
    *** cells: MCF10A in BMS
    *** reqs: Muc1 [RNAi]
    *** source: 18037881-Fig-4b
  *** Ikk2[phosAb] phos(S181)[phosAb] is increased irt Tnf (30 min)
    *** cells: mMECs in BMS
    *** reqs: Muc1 [RNAi]
    *** source: 18037881-Fig-4c

Nemo--  ubiq

  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: mESCs in BMS
    *** times: 0 10++ 30+ 60+ min
    *** reqs: Nemo [KO]
    *** source: 10805784-Fig-4a
  *** xNemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: JURKAT<Pten~null> in BMS
    *** times: 0 5+ 10+ min
    *** inhibited by: xNemo(Y308S)"Ubiq-binding-mut" [substitution]
    *** source: 16603398-Fig-7g
  *** Ikk2[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: JURKAT<Pten~null><Nemo~null><xNemo> in BMS
    *** reqs: xNemo [omission]
    *** inhibited by: xNemo(L320P) [substitution]
    *** inhibited by: xNemo(D311N) [substitution]
    *** source: 16547522-Fig-3a
  *** xNemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: JM452<Nemo~null> in BMS
    *** inhibited by: xNemo(C417R) [substitution]
    *** source: 12867425-Fig-5d

Nik--  ????

  *** Ikks[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS
    *** does not req: Nik [KO]
    *** source: 11251123-Fig-1c
  *** xIkk1[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (tnr)
    *** cells: COS in BMS
    *** bkg inhibited by: xNik(K429A/K430A) [addition]
    *** source: 10779355-Fig-5b
  *** xIkk1[tAb]IP phos[32Pi] is increased irt Tnf (5 min)
    *** cells: COS in BMS
    *** inhibited by: xNik(K429A/K430A) [addition]
    *** source: 10779355-Fig-2a
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (tnr)
    *** cells: COS in BMS
    *** inhibited by: xNik(K429A/K430A) [addition]
    *** source: 10779355-Fig-5a
  *** xIkk2[tAb]IP phos[32Pi] is increased irt Tnf (5 min)
    *** cells: COS in BMS
    *** bkg inhibited by: xNik(K429A/K430A) [addition]
    *** source: 10779355-Fig-2c

not P38a--

  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: HELA in BMS
    *** times: 0 5++ 10+ min
    *** does not req: P38a [RNAi]
    *** source: 15590691-Fig-7c

not Pi3k--

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (20 min)
    *** cells: mEFs in BMS
    *** partially reqs: Ikk2 [KO]
    *** unaffected by: LY294002 [addition]
    *** source: 11733537-Fig-4b

Pkci--

  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (7 min)
    *** cells: HEK293 in BMS
    *** inhibited by: xPkci(mnr)"DN" [addition]
    *** source: 10022904-Fig-5d

not Pkcz--

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 5++ 10++ 20+ min
    *** does not req: Pkcz [KO]
    *** source: 11684013-Fig-3a
  *** xIkk1[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (7 min)
    *** cells: HEK293 in BMS
    *** inhibited by: xPkcz(mnr)"DN" [addition]
    *** source: 10022904-Fig-5e
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (7 min)
    *** cells: HEK293 in BMS
    *** inhibited by: xPkcz(mnr)"DN" [addition]
    *** source: 10022904-Fig-5b,5c

not Pkr--

  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 10+ 30 min
    *** does not req: Pkr [KO]
    *** source: 10648614-Fig-6b

Ppp2cb--  inhibition (dephosphorylation)

  *** Ikk1[Ab]IP phos[32Pi] is increased irt Tnf (15 min)
    *** cells: HELA in BMS
    *** inhibited by: xPpp2cb [addition]
    *** unaffected by: xPpp2cb(R179G)"phosphatase-defective-mut" [addition]
    *** source: 14585847-Fig-2a

Ripk1--

  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 10++ 30+ 60+ min
    *** reqs: Ripk1 [KO]
    *** source: 15175328-Fig-2a
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: JURKAT<Pten~null> in BMS
    *** times: 0 5+ 10+ min
    *** reqs: Ripk1 [KO]
    *** source: 16603398-Fig-2a
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS
    *** partially reqs: Ripk1 [KO]
    *** source: 10795740-Fig-1c
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS
    *** reqs: Ripk1 [KO]
    *** source: 10795740-Fig-2a
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: mEFs<Ripk1~null><xRipk1> in BMS
    *** reqs: xRipk1 [omission]
    *** source: 10795740-Fig-2a
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: mEFs<Ripk1~null><xRipk1> in BMS
    *** reqs: xRipk1 [omission]
    *** unaffected by: xRipk1(K45A) [substitution]
    *** source: 10795740-Fig-6a
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 5++ 15+ min
    *** reqs: Ripk1 [KO]
    *** source: 10795740-Fig-1a
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS
    *** reqs: Ripk1 [KO]
    *** source: 10795740-Fig-2c
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: mEFs<Ripk1~null><xRipk1> in BMS
    *** reqs: xRipk1 [omission]
    *** source: 10795740-Fig-2c

  ----  but not Ripk1-kinase activity ----

  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs<Ripk1~null><xRipk1> in BMS
    *** times: 0 10++ 30+ 60+ min
    *** unaffected by: xRipk1(D138N)"KD" [substitution]
    *** source: 15175328-Fig-2a

  ---- Ripk1-ubiq ---

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: JURKAT<Pten~null><Ripk1~null><xRipk1> in BMS
    *** times: 0 5+ 10+ min
    *** inhibited by: xRipk1(K377R)"Ubiq-deficient-mut" [substitution]
    *** unaffected by: xRipk1(K305R/K306R) [substitution]
    *** unaffected by: xRipk1(K396R) [substitution]
    *** unaffected by: xRipk1(K565R) [substitution]
    *** source: 16603398-Fig-2a
  *** xRipk1[Ab] copptby[WB] Nemo[Ab] is increased irt Tnf (times)
    *** cells: JURKAT<Pten~null><Ripk1~null><xRipk1> in BMS
    *** times: 0 2+ 5+ min
    *** inhibited by: xRipk1(K377R)"Ubiq-deficient-mut" [substitution]
    *** source: 16603398-Fig-4a

Tab1-- or Tab2--

  *** Ikk2[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 15+ 30+ min
    *** does not req: Tab1 [KO]
    *** does not req: Tab2 [KO]
    *** source: 16260493(D)
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: HEK293 in BMS
    *** times: 0 10++ 20+ min
    *** partially reqs: Tab2 [RNAi]
    *** source: 15327770(D)
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: HEK293 in BMS
    *** times: 0 5+ 10+ min
    *** reqs: Tab1 and/or Tab2 [RNAi]
    *** source: 16603398-Fig-5d
  *** Ikk2[phosAb] phos(S181)[phosAb] is increased irt Tnf (30 min)
    *** cells: MCF10A in BMS
    *** does not req: Tab1 [RNAi]
    *** source: 18037881-Fig-S5
  *** Ikk2[phosAb] phos(S181)[phosAb] is increased irt Tnf (30 min)
    *** cells: MCF10A in BMS
    *** reqs: Tab2 [RNAi]
    *** source: 18037881-Fig-5b
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: HELA in BMS
    *** does not req: Tab1 [RNAi]
    *** source: 15590691-Fig-6a
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: HELA in BMS
    *** does not req: Tab2 [RNAi]
    *** source: 15590691-Fig-6b

Tak1--

  *** Ikk2[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 15+ 30+ min
    *** reqs: Tak1 [KO]
    *** source: 16260493(D)
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: HEK293 in BMS
    *** times: 0 5+ 10+ min
    *** reqs: Tak1 [RNAi]
    *** source: 16603398-Fig-5d
  *** Ikk2[phosAb] phos(S181)[phosAb] is increased irt Tnf (30 min)
    *** cells: MCF10A in BMS
    *** reqs: Tak1 [RNAi]
    *** source: 18037881-Fig-5a
  *** Ikk2[Ab] copptby[WB] xTak1[His] is increased irt Tnf (times)
    *** cells: HEK293T<xTak1> in BMS
    *** times: 0 5+ 10++ 15++ min
    *** inhibited by: xTak1(K158R) [substitution]
    *** source: 20038579-Fig-S3a
  *** Ikks[phosAb] phos(sitenr)[phosAb] is increased irt Tnf (times)
    *** cells: mEFs<Tak1~null><xTak1> in BMS
    *** times: 0 5+ 10++ 15++ 30++ min
    *** reqs: xTak1 [omission]
    *** partially inhibited by: xTak1(K158R) [addition]
    *** source: 20038579-Fig-7a
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: HELA in BMS
    *** partially reqs: Tak1 [RNAi]
    *** source: 15590691-Fig-5d
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (30 min)
    *** cells: mEFs in BMS
    *** partially reqs: Tak1 [KO]
    *** source: 20932476-Fig-1g

not Tank--

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: HEK293 in BMS
    *** times: 0 5+ 10++ 20++ 30 min
    *** does not req: Tank [RNAi]
    *** source: 16336209-Fig-7c

not Tpl2--

  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (15 min)
    *** cells: HEK293 in BMS
    *** unaffected by: xTpl2(K167M) [addition]
    *** source: 15670770-Fig-1c

inhibition by Tnfaip3--

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 10++ 30+ 60+ 90+ 120+ min
    *** comment: decrease after 60 min partially reqs: Tnfaip3 [KO]
    *** source: 11009421-Fig-3e

TnfR1--

  *** Nemo[Ab]IP IVKA(Tank)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 5 10+++ 20++ 30+ min
    *** reqs: TnfR1 [KO]
    *** source: 16336209-Fig-4a

Traf2--

  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS
    *** partially reqs: Traf2 [KO]
    *** source: 10795740-Fig-1c
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS
    *** reqs: Traf2 [KO]
    *** source: 10795740-Fig-2b
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: mEFs<Traf2~null><xTraf2> in BMS
    *** reqs: xTraf2 [omission]
    *** source: 10795740-Fig-2b
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 5++ 15+ min
    *** partially reqs: Traf2 [KO]
    *** source: 10795740-Fig-1a
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS
    *** reqs: Traf2 [KO]
    *** source: 10795740-Fig-2c
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: mEFs<Traf2~null><xTraf2> in BMS
    *** reqs: xTraf2 [omission]
    *** source: 10795740-Fig-2c
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 10++ 30+ min
    *** comment: reqs Traf2 and/or Traf5 [DKO] (at 10 min only)
    *** source: 11479302-Fig-1b

Ube1--

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: U2OS in BMS
    *** times: 0 5+ 10+ min
    *** partially reqs: Ube1 [RNAi]
    *** comment: the amount of Ikk2 coppt by Nemo is decreased by Ube1 [RNAi]
    *** source: 19854138-Fig-S3d

Ube2d3--

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: U2OS<Ube2ds~null><xUbe2d3> in BMS
    *** times: 0 5+ 15++ min
    *** inhibited by: xUbe2d3(C85A) [substitution]
    *** source: 19854138-Fig-4b

Ube2ds--

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: U2OS in BMS
    *** times: 0 5+ 15++ min
    *** partially reqs: Ube2ds [RNAi]
    *** source: 19854138-Fig-4a

not Ubc13--

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: U2OS in BMS
    *** times: 0 5+ 15++ min
    *** does not req: Ubc13 [RNAi]
    *** source: 19854138-Fig-3a

Ubiq--  not K63

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: U2OS in BMS
    *** times: 0 5+ 15+ min
    *** reqs: Ubiq [RNAi]
    *** source: 19854138-Fig-2a,2c
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: U2OS<Ubiq~null><xUbiq> in BMS
    *** times: 0 5+ 15+ min
    *** unaffected by: xUbiq(K63R) [substitution]
    *** source: 19854138-Fig-2c
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: U2OS<Ubiq~null><xUbiq(K63R)> in BMS
    *** source: 19854138-Fig-S5a

not Usp21--

  *** Ikks[phosAb] phos(sitenr)[phosAb] is increased irt Tnf (times)
    *** cells: HELA in BMS
    *** times: 0 5 10+ 15++ 30+ min
    *** does not req: Usp21 [RNAi]
    *** source: 19910467-Fig-4b

====================
inhibitory proteins
====================

  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: HEK293T in BMS
    *** inhibited by: xCyld [addition]
    *** source: 12917691-Fig-S4a
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (20 min)
    *** cells: HEK293 in BMS
    *** repressed by: Cyld [RNAi]
    *** source: 12917690-Fig-2b
  *** Ikk2[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (30 min)
    *** cells: CMT93 in BMS
    *** inhibited by: Tgfb1 (tnr) [stim]
    *** comment: inhibition by Tgfb1 reqs Smad7 [RNAi]
    *** source: 17384642-Fig-S4
  *** Ikk2[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: MCF7 in BMS
    *** times: 0 5+ 15++ 30++ min
    *** inhibited by: xSmad7 [addition]
    *** source: 17384642-Fig-S2d

====================
Ikk1 Phosphorylation
====================

  *** Ikk1[Ab]IP phos[32Pi] is increased irt Tnf (times)
    *** cells: HELA in BMS
    *** times: 0 10++ 20++ 30+ min
    *** source: 10195894-Fig-1a
  *** xIkk1[tAb]IP phos[32Pi] is increased irt Tnf (5 min)
    *** cells: COS in BMS
    *** source: 10779355-Fig-1b

Ikk1 phos irt Tnf requires Ikk1 kinase activity and phos-sites S176/S180

  *** Ikk1[Ab]IP phos[32Pi] is increased irt Tnf (5 min)
    *** cells: COS in BMS
    *** source: 10779355-Fig-1a
  *** xIkk1(K44M)[tAb]IP phos[32Pi] is unchanged irt Tnf (5 min)
    *** cells: COS in BMS
    *** source: 10779355-Fig-2b
  *** xIkk1(S176A/S180A)[tAb]IP phos[32Pi] is unchanged irt Tnf (5 min)
    *** cells: COS in BMS
    *** source: 10779355-Fig-2b

Ikk1 phos irt Tnf does not require Ikk2

  *** xIkk1[tAb]IP phos[32Pi] is increased irt Tnf (5 min)
    *** cells: COS in BMS
    *** unaffected by: xIkk2(S177A/S181A) [addition]
    *** unaffected by: xIkk2(K44M) [addition]
    *** source: 10779355-Fig-2a

Ikk1 phos sites S176/S180 are not required for IKK complex activation

  *** xIkk1[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: HELA in BMS
    *** unaffected by: xIkk1(S176A/S180A) [substitution]
    *** unaffected by: xIkk1(S176A) [substitution]
    *** unaffected by: xIkk1(S180A) [substitution]
    *** source: 10195894-Fig-2b

Ikba is phosed on S32/S36 by IKK complex

  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: THP1 in BMS
    *** times: 0 3++ 5++ 10+ 30 min
    *** inhibited by: rIkba(S32A/S36A) [substitution]
    *** source: 10477696-Fig-2,5b


====================
Ikk2 Phosphorylation
====================

Ikk2 phos

  *** Ikk2[Ab]IP phos[32Pi] is increased irt Tnf (15 min)
    *** cells: HELA in BMS
    *** inhibited by: xPpp2cb [addition]
    *** unaffected by: xPpp2cb(R179G)"phosphatase-defective-mut" [addition]
    *** source: 14585847-Fig-2a
  *** xIkk2[tAb]IP phos[32Pi] is increased irt Tnf (5 min)
    *** cells: COS in BMS
    *** source: 10779355-Fig-1b
  *** Ikk2[Ab]IP phos[32Pi] is increased irt Tnf (times)
    *** cells: HELA in BMS
    *** times: 0 10++ 20++ 30+ min
    *** source: 10195894-Fig-1a

on S181 ???

  *** Ikk2[phosAb] phos(S181)[phosAb] is increased irt Tnf (times)
    *** cells: MCF7 in BMLS
    *** times: 0+ 5++ 10++ 20+ 30+ min
    *** source: 17693255-Fig-1a
  *** Ikk2[phosAb] phos(S181)[phosAb] is unchanged irt Tnf (times)
    *** cells: MDAMB453 in BMLS
    *** times: 0+ 5+ 10+ 20+ 30+ min
    *** source: 17693255-Fig-1a

Ikk2 phos irt Tnf requires Ikk2 kinase activity and phos-sites S177/S181

  *** xIkk2[tAb]IP phos[32Pi] is increased irt Tnf (5 min)
    *** cells: COS in BMS
    *** inhibited by: xIkk2(S177A/S181A) [addition]
    *** partially inhibited by: xIkk2(K44M) [addition]
    *** source: 10779355-Fig-2c
  *** xIkk2(K44M)[tAb]IP phos[32Pi] is unchanged irt Tnf (5 min)
    *** cells: COS in BMS
    *** source: 10779355-Fig-2d
  *** xIkk2(S177A/S181A)[tAb]IP phos[32Pi] is unchanged irt Tnf (5 min)
    *** cells: COS in BMS
    *** source: 10779355-Fig-2d

Ikk2 phos sites S177/S181 are required for autophosphorylation

  *** xIkk2[tAb]IP IVKA(auto)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** unaffected by: xIkk2(K44M)"KD" [substitution]
    *** inhibited by: xIkk2(K44M/S177A/S181A) [substitution]
    *** source: 14636585-Fig-5a
  *** xIkk2[tAb]IP IVKA(auto)[32P-ATP] is increased irt Tnf (30 min)
    *** cells: mEFs in BMS "on gelatin"
    *** unaffected by: xIkk2(K44M)"KD" [substitution]
    *** inhibited by: xIkk2(K44M/S177A/S181A) [substitution]
    *** source: 14636585-Fig-5b

Ikk2 phos sites S177/S181 are not required for IKK complex activity

  *** xIkk1[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (tnr)
    *** cells: COS in BMS
    *** unaffected by: xIkk2(S177A/S181A) [addition]
    *** source: 10779355-Fig-5b

====================
Ikks Phosphorylation
====================

  *** Ikks[phosAb] phos(SLCTS)[phosAb] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 2+ 5++ 10++ 30+ min
    *** unaffected by: SmacMimetic [addition]
    *** source: 18635759-Fig-S11

===================
effect of chemicals
===================

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (tnr)
    *** cells: HELA in BMS
    *** inhibited by: Geldanamycin [addition]
    *** source: 11864612-Fig-5c
  *** Nemo[Ab]IP IVKA(Rela)[32P-ATP] is increased irt Tnf (20 min)
    *** cells: mEFs in BMS
    *** inhibited by: LY294002 [addition]
    *** source: 11733537-Fig-4b
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (7 min)
    *** cells: HEK293 in BMS
    *** unaffected by: Bisindolylmaleimide-I [addition]
    *** source: 10022904-Fig-5a
  *** Ikk2[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: mBMMs in BMS
    *** inhibited by: Piceatannol [addition]
    *** source: 16291755-Fig-5c
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: U2OS in BMS
    *** times: 0 5+ 10+ min
    *** partially inhibited by: SmacMimetic [addition]
    *** source: 19854138-Fig-7a
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (15 min)
    *** cells: CEM in BMS
    *** unaffected by: LMB [pretreatment]
    *** source: 14651848-Fig-7a

=========
no extras
=========

  *** xIkk1[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: HELA in BMS
    *** source: 9819420-Fig-3a
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf
    *** cells: HELA in BMS
    *** times: 0 1 2+ 5++ 10+++ 15+++ 20++ 30+ 60+ min
    *** source: 10195894-Fig-1a
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: THP1 in BMS
    *** times: 0 5++ 10++ 20++ 30+ 40+ 50+ 60+ 75 90 min
    *** source: 10455128-Fig-1a
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: hPBMCs in BMS
    *** times: 0 2+ 5++ 30 min
    *** source: 10455128-Fig-2a
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: HELA in BMS
    *** times: 0 2+ 5++ 10++ 20+ min
    *** source: 10521409-Fig-4a
  *** Ikk1[Ab]IP IVKA(Rela)[32P-ATP] is increased irt Tnf (times)
    *** cells: HELA in BMS
    *** times: 0 2+ 5++ 10++ 20+ min
    *** source: 10521409-Fig-4a
  *** Ikks[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: HELA in BMS
    *** times: 0 5++ 15++ 30+ 60+ 120 min
    *** comment: reqs Ppp2bc [RNAi] for the decrease at later times
    *** source: 14585847-Fig-4b
  *** xIkk1[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: HEK293 in BMS
    *** source: 10346818-Fig-2f
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 15+ 180+ min
    *** source: 12387745-Fig-6a
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: HELA in BMS
    *** source: 9819420-Fig-3a
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: HELA in BMS
    *** source: 9819420-Fig-3b
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: HELA in BMS
    *** source: 10195894-Fig-2b
  *** Ikk2[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: THP1 in BMS
    *** source: 10477696-Fig-2
  *** Ikk2[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: hPBMCs in BMS
    *** times: 0 2 5+ 30 min
    *** source: 10455128-Fig-2a
  *** Ikk2[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: THP1 in BMS
    *** times: 0 2 5++ 15+ min
    *** source: 10455128-Fig-1a
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: HEK293 in BMS
    *** source: 10346818-Fig-4d
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (5 min)
    *** cells: HELA in BMS
    *** source: 14713952-Fig-2c
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: HELA in BMS
    *** times: 0 2++ 5+++ 10+++ 20+ min
    *** source: 15327770(D)
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: SAOS2 in BMS
    *** source: 15073170-Fig-3c
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 15+ 180+ min
    *** source: 12387745-Fig-6a
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 2+ 3++ 5+++ min
    *** source: 10795740-Fig-4e
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: U2OS in BMS
    *** source: 19854138-Fig-S5a
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: mBMMs in BMS
    *** times: 0 2++ 5+ 10+ 15 30 min
    *** source: 15199157-Fig-4
  *** Nemo[Ab]IP IVKA(Nfkb1)[32P-ATP] is increased irt Tnf (times)
    *** cells: mBMMs in BMS
    *** times: 0 7++ 15+ min
    *** source: 15199157-Fig-9a
  *** xIkk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (15 min)
    *** cells: mEFs<Ikk1~null><xIkk1> in BMS
    *** inhibited by: xIkk1(1-737)"delNBD" [substitution]
    *** source: 19666475-Fig-7a
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: HELA in BMLS
    *** times: 0 5+ 10+ 30+ 60+ min
    *** source: 10783893-Fig-4d
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: T98G in BMS
    *** times: 0 12++ 30+ 60+ 90 120 240 min
    *** source: 10648614-Fig-5b


=================
Nemo ubiq irt Tnf
=================

Ciap1--

  *** Nemo[Ab]IP ubiq[UbAb] is increased irt Tnf (times)
    *** cells: HEK293T in BMS
    *** times: 0 2+ 5+ min
    *** reqs: Ciap1 [RNAi]
    *** source: 12867425-Fig-4j


Nemo features

  *** xNemo[Ab]IP ubiq[UbAb] is increased irt Tnf (times)
    *** cells: JM452<Nemo~null> in BMS
    *** times: 0+ 2+ 5++ min
    *** bkg inhibited by: xNemo(C417R) [substitution]
    *** source: 12867425-Fig-5b

no extras

  *** Nemo[Ab]IP ubiq[UbAb] is increased irt Tnf (5 min)
    *** cells: HELA in BMS
    *** source: 12867425-Fig-1b
  *** Nemo[Ab]IP upshift[WBMS] is increased irt Tnf (times)
    *** cells: HELA in BMS
    *** times: 0 2+ 5++ 10+ 20+ 30+ min
    *** source: 12867425-Fig-1a

