=====================================
Proteins Required for mRNA expression
=====================================

Ampka1--

  *** IL8-gene mRNA[RTPCR] is increased irt Tnf (12 hr)
    *** cells: THP1 in BMS
    *** reqs: Ampka1 [RNAi]
    *** source: 22833096-Fig-6b

not Cezanne--

  *** IL8-gene mRNA[RTPCR] is increased irt Tnf (2 hr)
    *** cells: hUVECs in BMS
    *** repressed by: Cezanne [RNAi]
    *** source: 18178551-Fig-2c

Erc1--

  *** IL8-gene mRNA[RTPCR] is increased irt Tnf (times)
    *** cells: HELA in BMS
    *** times: 0 30+ 60+ 120 min
    *** reqs: Erc1 [RNAi]
    *** source: 15218148-Fig-3b

not Ikke--

  *** IL8[Ab] secretion[ELISA] is increased irt Tnf (times)
    *** cells: HEK293 in BMS
    *** times: 0 2+ 4++ 6++ 8++ 24++ hr
    *** unaffected by: xIkke(K38M)"DN" [addition]
    *** source: 16199137-Fig-2a

Irak1--

  *** IL8-gene mRNA[RTPCR] is increased irt Tnf (18 hr)
    *** cells: hUVECs in BMS
    *** partially reqs: Irak1 [RNAi]
    *** source: 19181383-Fig-3b
  *** IL8-gene mRNA[RTPCR] is increased irt Tnf (18 hr)
    *** cells: hUVECs in BMS
    *** partially reqs: Irak1 and/or Irak4 [RNAi]
    *** source: 19181383-Fig-3b
  *** IL8-gene mRNA[Northern] is increased irt Tnf (6 hr)
    *** cells: HEK293 in BMS
    *** repressed by: Irak1 [null-mut]
    *** source: 10373513-Fig-3c
  -------------------------------------------------------------
  *** IL8[Ab] secretion[ELISA] is increased irt Tnf (18 hr)
    *** cells: hUVECs in BMS
    *** reqs: Irak1 [RNAi]
    *** source: 19181383-Fig-2a


not Irak4--

  *** IL8-gene mRNA[RTPCR] is increased irt Tnf (18 hr)
    *** cells: hUVECs in BMS
    *** does not req: Irak4 [RNAi]
    *** source: 19181383-Fig-3a
  -------------------------------------------------------------
  *** IL8[Ab] secretion[ELISA] is increased irt Tnf (18 hr)
    *** cells: hUVECs in BMS
    *** does not req: Irak4 [RNAi]
    *** source: 19181383-Fig-2a

not Myd88--

  *** IL8-gene mRNA[Northern] is increased irt Tnf (6 hr)
    *** cells: HEK293 in BMS
    *** does not req: Myd88 [null-mut]
    *** source: 10373513-Fig-3c

not Peli2--

  *** IL8-gene promo-reporter[Luc] is increased irt Tnf (5 hr)
    *** cells: C3H10T12 in BMS
    *** does not req: Peli2 [AS-DNA]
    *** source: 12370331-Fig-3c

Tank--

  *** IL8-gene mRNA[RTPCR] is increased irt Tnf (tnr)
    *** cells: HELA in BMS
    *** partially reqs: Tank [RNAi]
    *** source: 16336209-Fig-8

chemicals

  *** IL8[Ab] secretion[ELISA] is increased irt Tnf (15 hr)
    *** cells: RPE1 in BMS
    *** unaffected by: Leupeptin [pretreatment]
    *** unaffected by: Z-FA-FMK [pretreatment]
    *** source: 22611194-Fig-2e

Question:  There are no datums in which Irak1 is required for
any pre-translation events irt Tnf.  Could the requirement for Irak1 for IL8
mRNA expression after 18 hr Tnf be due to upregulation of another cytokine
(such as IL1b) that does require Irak1?


==========
Inhibitors
==========

  *** IL8-gene promo-reporter[Luc] is increased irt Tnf (24 hr)
    *** cells: HEK293<xStat6> in BMS
    *** unaffected by: xSocs1 [addition]
    *** unaffected by: xSocs2 [addition]
    *** unaffected by: xSocs3 [addition]
    *** source: 15589317-Fig-3
  *** IL8-gene promo-reporter[Luc] is increased irt Tnf (24 hr)
    *** cells: HEK293<xStat6> in BMS
    *** unaffected by: xSocs1 [addition]
    *** unaffected by: xSocs1(F58A) [addition]
    *** unaffected by: xSocs2 [addition]
    *** partially inhibited by: xSocs3 [addition]
    *** partially inhibited by: xSocs3(F25A) [addition]
    *** source: 15589317-Fig-8







