=====================================
Proteins Required for mRNA expression
=====================================

not Atf2--

  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (24 hr)
    *** cells: 3T3L1 in BMS "differentiated into adipocytes"
    *** does not req: Atf2 [RNAi]
    *** source: 17500068-Fig-6b

Msk1-- and/or Msk2--

  *** IL6-gene mRNA[MicroArray] is increased irt Tnf (4 hr)
    *** cells: mEFs in BMS
    *** partially reqs: Msk1 and/or Msk2 [DKO]
    *** source: 12628924-Fig-8e

Ikba--

  *** IL6-gene mRNA[Northern] is increased irt Tnf (45 min)
    *** cells: mEFs in BMS
    *** inhibited by: xIkba(mnr)"non-phosphorylatable" [addition]
    *** source: 15545623-Fig-1b

Ikk1--

  *** IL6-gene mRNA[Northern] is increased irt Tnf (4 hr)
    *** cells: mEFs in BMS
    *** reqs: Ikk1 [KO]
    *** source: 11733537-Fig-1a

Ikk2--

  *** IL6-gene mRNA[Northern] is increased irt Tnf (4 hr)
    *** cells: mEFs in BMS
    *** reqs: Ikk2 [KO]
    *** source: 11733537-Fig-1a

not Ikke--

  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (24 hr)
    *** cells: mEFs in BMS
    *** does not req: Ikke [KO]
    *** source: 15210742-Fig-2a

not Irak1--  is the difference between mRNA and secretion significant?

  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (18 hr)
    *** cells: hUVECs in BMS
    *** does not req:  Irak1 and/or Irak4 [RNAi]
    *** source: 19181383-Fig-3b
  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (18 hr)
    *** cells: hUVECs in BMS
    *** does not req: Irak1 [RNAi]
    *** source: 19181383-Fig-3b
  *** IL6-gene mRNA[Northern] is increased irt Tnf (3 hr)
    *** cells: mEFs in BMLS
    *** does not req: Irak1 [KO]
    *** source: 9625767(D)
  ---------------------------------------------------------
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (18 hr)
    *** cells: hUVECs in BMS
    *** reqs: Irak1 [RNAi]
    *** source: 19181383-Fig-2a

not Irak4--  is the difference between mRNA and secretion significant?

  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (18 hr)
    *** cells: hUVECs in BMS
    *** does not req: Irak4 [RNAi]
    *** source: 19181383-Fig-3a
  ----------------------------------------------------------
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (tnr)
    *** cells: mEFs in BMS
    *** reqs: Irak4 [KO]
    *** source: 11923871(D)
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (18 hr)
    *** cells: hUVECs in BMS
    *** does not req: Irak4 [RNAi]
    *** source: 19181383-Fig-2a
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 16+ 24++ 40+++ hr
    *** unaffected by: Irak4(K213A/K214A)"KD" [addition]
    *** source: 17337443-Fig-6a

Jnk1-- and/or Jnk2--  ???

  *** IL6-gene mRNA[Northern] is increased irt Tnf (45 min)
    *** cells: mEFs in BMS
    *** bkg reqs: Jnk1 and/or Jnk2 [DKO]
    *** source: 15545623-Fig-1b

not Jun--

  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (24 hr)
    *** cells: 3T3L1 in BMS "differentiated into adipocytes"
    *** does not req: Jun [RNAi]
    *** source: 17500068-Fig-6b

Myd88--  ???

  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (20 hr)
    *** cells: hUVECs in BMS
    *** inhibited by: xMyd88(del(1-53)/F56N)"DN" [addition]
    *** source: 14630816(D)
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (20 hr)
    *** cells: hSNFs in BMS
    *** inhibited by: xMyd88(del(1-53)/F56N)"DN" [addition]
    *** source: 14630816(D)


not Nfkbiz--

  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (24 hr)
    *** cells: mEFs in BMS
    *** does not req: Nfkbiz [KO]
    *** source: 15241416-Fig-2e

Pkcz--  ????

  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (24 hr)
    *** cells: mEFs in BMS
    *** partially reqs: Pkcz [KO]
    *** source: 12881425-Fig-5a

Rela--  ???

  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (24 hr)
    *** cells: mEFs<xRela> in BMS
    *** partially inhibited by: xRela(S311A) [substitution]
    *** source: 12881425-Fig-5b
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (24 hr)
    *** cells: mEFs<Rela~null><xRela> in BMS
    *** reqs: xRela [omission]
    *** inhibited by: xRela(S276A) [substitution]
    *** unaffected by: xRela(S529A) [substitution]
    *** unaffected by: xRela(S536A) [substitution]
    *** unaffected by: xRela(S529A/S536A) [substitution]
    *** source: 12559944-Fig-2a

Ripk1--

  *** IL6-gene mRNA[RPA] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 2+ 24++ hr
    *** reqs: Ripk1 [KO]
    *** source: 14585994-Fig-3c
  -----------------------------------------------------------
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (24 hr)
    *** cells: mEFs in BMS
    *** reqs: Ripk1 [KO]
    *** source: 14585994-Fig-3e
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (24 hr)
    *** cells: mEFs<Ripk1~null><xRipk1> in BMS
    *** reqs: xRipk1 [omission]
    *** source: 14585994-Fig-3e
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (24 hr)
    *** cells: mEFs in BMS
    *** reqs: Ripk1 [KO]
    *** source: 14585994-Fig-3a
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (24 hr)
    *** cells: mEFs in BMS
    *** reqs: Ripk1 [KO]
    *** source: 15175328-Fig-2c
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (24 hr)
    *** cells: mEFs<Ripk1~null><xRipk1> in BMS
    *** comment: bkg is increased by xRipk1(D138N)"DN" [substitution]
    *** source: 15175328-Fig-2c

not Rnf11--

  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (tnr)
    *** cells: THP1 in BMS
    *** repressed by: Rnf11 [RNAi]
    *** source: 19131965-Fig-3d

Smad2-- ???

  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (6 hr)
    *** cells: mBMMs in BMLS
    *** unaffected by: xSmad2 [addition]
    *** source: 17384642-Fig-4a
  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (6 hr)
    *** cells: mBMMs in BMLS
    *** unaffected by: xSmad2 [addition]
    *** source: 17384642-Fig-4b
  ---------------------------------------------------------
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (6 hr)
    *** cells: mBMMs in BMLS
    *** unaffected by: xSmad2 [addition]
    *** source: 17384642-Fig-4c


not Smad6--

  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (6 hr)
    *** cells: mPECs in BMLS
    *** does not req: Smad6 [RNAi]
    *** source: 17384642-Fig-4e
  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (6 hr)
    *** cells: mPECs in BMLS
    *** does not req: Smad6 [RNAi]
    *** source: 17384642-Fig-4d
  ---------------------------------------------------------
  *** IL6[Ab] prot-exp[WB] is increased irt Tnf (6 hr)
    *** cells: mPECs in BMLS
    *** does not req: Smad6 [RNAi]
    *** source: 17384642-Fig-4h
  ---------------------------------------------------------
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (6 hr)
    *** cells: mPECs in BMLS
    *** partially reqs: Smad6 [RNAi]
    *** source: 17384642-Fig-4i

Syk--

  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (18 hr)
    *** cells: hBECs in BMS
    *** partially reqs: Syk [RNAi]
    *** source: 15557085-Fig-7d
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (18 hr)
    *** cells: hBECs in BMS "on Fibronectin"
    *** partially reqs: Syk [RNAi]
    *** source: 15557085-Fig-7d
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (18 hr)
    *** cells: hBECs in BMS
    *** inhibited by: Piceatannol [addition]
    *** source: 15557085-Fig-7d
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (18 hr)
    *** cells: hBECs in BMS "on Fibronectin"
    *** inhibited by: Piceatannol [addition]
    *** source: 15557085-Fig-7d

not Tab2--

  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (16 hr)
    *** cells: mEFs in BMS
    *** does not req: Tab2 [KO]
    *** source: 12556483-Fig-3b

Tak1--

  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (8 hr)
    *** cells: mEFs<Tak1~null><xTak1> in BMS
    *** reqs: xTak1 [omission]
    *** inhibited by: xTak1(K158R) [addition]
    *** source: 20038579-Fig-8a
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (times)
    *** cells: mEFs<Tak1~null><xTak1> in BMS
    *** times: 0 8+ 12+ 24+ hr
    *** reqs: xTak1 [omission]
    *** inhibited by: xTak1(K158R) [addition]
    *** source: 20038579-Fig-8c

not Tbk1--

  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (24 hr)
    *** cells: mEFs in BMS
    *** does not req: Tbk1 [KO]
    *** source: 15210742-Fig-2a

not Tirap--

  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (20 hr)
    *** cells: hUVECs in BMS
    *** unaffected by: xTirap(P125H)"DN" [addition]
    *** source: 14630816(D)
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (20 hr)
    *** cells: hSNFs in BMS
    *** unaffected by: xTirap(P125H)"DN" [addition]
    *** source: 14630816(D)

Tpl2--

  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (tnr)
    *** cells: mEFs in BMS
    *** partially reqs: Tpl2 [KO]
    *** source: 15833743-Fig-6E

=========
Chemicals
=========

  *** IL6-gene mRNA[MicroArray] is increased irt Tnf (4 hr)
    *** cells: L929SA in BMS
    *** inhibited by: SB203580 + PD98059 [addition]
    *** source: 12628924-Fig-8e

================================
Proteins that repress expression
================================

Mlk3--

  *** IL6-gene mRNA[RPA] is increased irt Tnf (24 hr)
    *** cells: mEFs in BMS
    *** repressed by: Mlk3 [KO]
    *** source: 15831472(D)

Smad7-- ???

  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (6 hr)
    *** cells: mBMMs in BMLS
    *** inhibited by: xSmad7 [addition]
    *** source: 17384642-Fig-4b
  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (6 hr)
    *** cells: mBMMs in BMLS
    *** inhibited by: xSmad7 [addition]
    *** source: 17384642-Fig-4a
  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (6 hr)
    *** cells: mPECs in BMLS
    *** does not req: Smad7 [RNAi]
    *** source: 17384642-Fig-4d
  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (6 hr)
    *** cells: mPECs in BMLS
    *** does not req: Smad7 [RNAi]
    *** source: 17384642-Fig-4e
  ---------------------------------------------------------
  *** IL6[Ab] prot-exp[WB] is increased irt Tnf (6 hr)
    *** cells: mPECs in BMLS
    *** does not req: Smad7 [RNAi]
    *** source: 17384642-Fig-4h
  ---------------------------------------------------------
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (6 hr)
    *** cells: mBMMs in BMLS
    *** bkg inhibited by: xSmad7 [addition]
    *** source: 17384642-Fig-4c
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (6 hr)
    *** cells: mPECs in BMLS
    *** partially reqs: Smad7 [RNAi]
    *** source: 17384642-Fig-4i

Tgfb--

  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (6 hr)
    *** cells: mBMMs in BMS
    *** comment: inhibited by Tgb1 (6 hr) [costim]
    *** source: 17384642-Fig-S3a
  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (6 hr)
    *** cells: mPECs in BMLS
    *** comment: bkg inhibited by Tgb1 (2 hr) [pretreatment]
    *** source: 17384642-Fig-4e
  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (6 hr)
    *** cells: CMT93 in BMS
    *** comment: inhibited by Tgb1 (6 hr) [costim]
    *** source: 17384642-Fig-S3a
  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (6 hr)
    *** cells: mPECs in BMLS
    *** comment: partially inhibited by TgbR1 (2 hr) [stim]
    *** comment: inhibition by Tgfb1 reqs Smad7 [RNAi]
    *** source: 17384642-Fig-4d
  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (6 hr)
    *** cells: CMT93 in BMS
    *** comment: inhibited by Tgb1 (6 hr) [costim]
    *** comment: inhibition by Tgfb1 reqs Smad7 [RNAi]
    *** source: 17384642-Fig-S3b
  -----------------------------------------------------------
  *** IL6[Ab] prot-exp[WB] is increased irt Tnf (6 hr)
    *** cells: mPECs in BMLS
    *** comment: inhibited by Tgb1 (2 hr) [pretreatment]
    *** source: 17384642-Fig-4h
  -----------------------------------------------------------
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (6 hr)
    *** cells: mPECs in BMLS
    *** comment: bkg inhibited by Tgb1 (2 hr) [pretreatment]
    *** source: 17384642-Fig-4i

Usp21--

  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (times)
    *** cells: HELA in BMS
    *** times: 0 30+ 60++ 120+ min
    *** repressed by: Usp21 [RNAi]
    *** source: 19910467-Fig-5a
  -----------------------------------------------------------
  *** IL6[Ab] secretion[ELISA] is increased irt Tnf (24 hr)
    *** cells: HELA in BMS
    *** times: 0 30+ 60++ 120+ min
    *** partially repressed by: Usp21 [RNAi]
    *** source: 19910467-Fig-5b

=========
no extras
=========

  *** IL6-gene mRNA[RTPCR] is increased irt Tnf (times)
    *** cells: 3T3L1 in BMS "differentiated into adipocytes"
    *** times: 0+ 2++ 6+++ 12+++ 24+++ 48++++ hr
    *** source: 17500068-Fig-3c
  *** IL6-gene mRNA[Northern] is increased irt Tnf (tnr)
    *** cells: L929SA in BMS
    *** source: 12628924-Fig-1a
  *** IL6-gene mRNA[Northern] is increased irt Tnf (tnr)
    *** cells: MDAMB231 in BMS
    *** source: 12628924-Fig-1a
  *** IL6-gene mRNA[Northern] is increased irt Tnf (tnr)
    *** cells: HELA in BMS
    *** source: 12628924-Fig-1a






















