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Smad4 is located in both cytoplasm and nucleus in resting cells
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  *** Smad4[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (60 min)
    *** cells: HACAT in BMS "treated with CHX"
    *** comment: Smad4 is partially located in nucleus before Tgfb1 treatment
    *** source: 12191474-Fig-5c
  *** Smad4[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS "treated with CHX"
    *** times: 0 60++ 90++ 120++ 180+ 240+ min
    *** comment: Smad4 is partially located in nucleus before Tgfb1 treatment
    *** source: 12191474-Fig-3b

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Smad4 retention in nucleus requires Smad2 phos on S464A/S465A/S467A
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  *** Smad4[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (1 hr)
    *** cells: MV1LU<xSmad2> in BMS
    *** inhibited by: xSmad2(S464A/S465A/S467A) [substitution]
    *** source: 9346966-Fig-6

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Other
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  *** Smad4[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS
    *** times: 0 10+ 60+ min
    *** source: 11074002-Fig-6a
  *** Smad4[Ab] infraction(nuclear)[WB] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS "treated with CHX"
    *** times: 0+ 30++ 60++ 120++ 180++ 360+ 540 min
    *** source: 11074002-Fig-7a
  *** Smad4[Ab] infraction(nuclear)[WB] is increased irt Tgfb1 (times)
    *** cells: NIH3T3 in BMS "treated with CHX"
    *** times: 0 30+ 60++ 120++ 180++ 360+ 540 min
    *** source: 11074002-Fig-7b
  *** Smad4[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS "treated with CHX"
    *** unaffected by: NaN3 + 2DOG [addition]
    *** source: 12191474-Fig-6
  *** Smad4[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (1 hr)
    *** cells: MV1LU in BMS
    *** source: 9311995-Fig-7
  *** xSmad4[tAb] locatedin(nucleus)[IHC] is increased irt Tgfb1 (1 hr)
    *** cells: C2C12<xSmad3> in BMS
    *** source: 11265759-Fig-5a
  *** xSmad4[tAb] locatedin(nucleus)[IHC] is increased irt Tgfb1 (tnr)
    *** cells: HEP3B in BMS
    *** inhibited by: xPdpk1 [addition]
    *** unaffected by: xPdpk1(mnr)"KD" [addition]
    *** repressed by: Pdpk1 [RNAi]
    *** source: 17327236-Fig-9c
  --- contradiction -------------------------------------------------------
  *** Smad4[Ab]IP infraction(nuclear)[WB] is unchanged irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS
    *** does not req: Ppm1a [RNAi]
    *** comment: nuclear fraction protocol is no longer in print
    *** source: 16751101-Fig-4d
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  *** Smad4[Ab] locatedin(cytoplasm)[IHC] is increased irt SB431542 (120 min)
    *** cells: HACAT in BMS "treated with CHX" "treated with Tgfb1 for 60 min"
    *** inhibited by: NaN3 + 2DOG [addition]
    *** source: 12191474-Fig-6
  *** Smad4[Ab] locatedin(cytoplasm)[IHC] is increased irt SB431542 (times)
    *** cells: HACAT in BMS "treated with CHX" "treated with Tgfb1 for 60 min"
    *** times: 0 30+ 60++ 120+++ 180++ min
    *** source: 12191474-Fig-3b
