=======================
Phosphorylation on T308
=======================

not Adam17--

  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Tgfb1 (90 min)
    *** cells: CHO in BMLS
    *** repressed by: Adam17 [RNAi]
    *** source: 19595713-Fig-4d

not Hras--

  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Tgfb2 (90 min)
    *** cells: AKR2B in BMLS
    *** unaffected by: Hras(S17N)"DN" [addition]
    *** source: 17440079-Fig-5a

not Mlst8--

  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Tgfb1 (4 hr)
    *** cells: mEFs in BMLS
    *** does not req: Mlst8 [KO]
    *** source: 19117990-Fig-4a

no extras

  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Tgfb1 (times)
    *** cells: AKR2B in BMLS
    *** times: 0 15 30 60+ 90+ 120++ min
    *** source: 16288034-Fig-1a

=======================
Phosphorylation on S473
=======================

not Dnm1--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (120 min)
    *** cells: AKR2B in BMLS
    *** unaffected by: xDnm1(K44A)"DN" [addition]
    *** source: 16288034-Fig-4a

Fak1--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (2 hr)
    *** cells: mEFs in BMLS
    *** reqs: Fak1 [KO]
    *** source: 21454615-Fig-1a
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (2 hr)
    *** cells: mEFs<Fak1~null><xFak1> in BMLS
    *** reqs: xFak1 [omission]
    *** source: 21454615-Fig-1a
  ---------- not Fak1 kinase activity -----------------
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: mEFs<Fak1~null><xFak1> in BMLS
    *** reqs: xFak1 [omission]
    *** unaffected by: xFak1(Y397F) [substitution]
    *** unaffected by: xFak1(P712A/P713A) [substitution]
    *** unaffected by: xFak1(P872A/P873A) [substitution]
    *** unaffected by: xFak1(Y397F/P712A/P713A) [substitution]
    *** unaffected by: xFak1(Y397F/P872A/P873A) [substitution]
    *** source: 21454615-Fig-8b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: mEFs<Fak1~null><xFak1> in BMLS
    *** reqs: xFak1 [omission]
    *** unaffected by: xFak1(Y397F) [substitution]
    *** unaffected by: xFak1(Y576F/Y577F) [substitution]
    *** unaffected by: xFak1(Y397F/K454R) [substitution]
    *** source: 21454615-Fig-6c
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: mEFs<Fak1~null><xFak1> in BMLS
    *** unaffected by: xFak1(P712A/P713A/P872A/P873A) [substitution]
    *** unaffected by: xFak1(Y397F/P712A/P713A/P872A/P873A) [substitution]
    *** source: 21454615-Fig-8c


Mlst8--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (4 hr)
    *** cells: mEFs in BMLS
    *** reqs: Mlst8 [KO]
    *** source: 19117990-Fig-4a

not Pak2--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (120 min)
    *** cells: AKR2B in BMLS
    *** unaffected by: xPak2(K278R)"DN" [addition]
    *** source: 16288034-Fig-5c

pi3krs--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (120 min)
    *** cells: COS7 in BMLS
    *** reqs: Pik3r? [AntiSense]
    *** source: 16288034-Fig-5d

Ppm1a--  ????

  *** Akt1[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (1 hr)
    *** cells: NIH3T3 in BMS
    *** unaffected by: xPpm1a [addition]
    *** source: 16751101-Fig-S8b

not Raptor--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (6 hr)
    *** cells: AKR2B in BMLS
    *** does not req: Raptor [RNAi]
    *** source: 19117990-Fig-4b

Rictor--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (6 hr)
    *** cells: AKR2B in BMLS
    *** partially reqs: Rictor [RNAi]
    *** source: 19117990-Fig-4b

not Smad2--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (2 hr)
    *** cells: HEPG2 in BMLS
    *** times: 0 1 3+ 6++ 12++ 24 hr
    *** unaffected by: xSmad2(D407E)"DN" [addition]
    *** source: 16014625(D)

not Smad3--

  *** xAkt1[Ab]IP phos(S473)[phosAb] is increased irt Tgfb1 (120 min)
    *** cells: mEFs<Smad3~null><xAkt1><xSmad2(S467A)> in BMLS
    *** source: 16288034-Fig-3

not Src-- Yes-- Fyn--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (4 hr)
    *** cells: mEFs<Src~null><Yes1~null><Fyn~null> in BMLS
    *** source: 21454615-Fig-5a

chemicals

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (times)
    *** cells: PC3U in BMS
    *** times: 0+ 30++ 720++ min
    *** inhibited by: SB203580 [addition]
    *** bkg inhibited by: LY294002 [addition]
    *** source: 15684397-Fig-5c
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (4 hr)
    *** cells: AKR2B in BMLS
    *** unaffected by: Rapamycin [pretreatment]
    *** source: 19117990-Fig-5b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (6 hr)
    *** cells: AKR2B in BMLS
    *** inhibited by: CHX [pretreatment]
    *** source: 19117990-Fig-2d
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (4 hr)
    *** cells: AKR2B in BMLS
    *** unaffected by: CytochalasinD [pretreatment]
    *** source: 21454615-Fig-5b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Hbegf (15 min)
    *** cells: AKR2B in BMLS
    *** unaffected by: U0126 [cotreatment]
    *** source: 23913859-Fig-3c
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Pdgfa + Pdgfb (15 min)
    *** cells: AKR2B in BMLS
    *** unaffected by: U0126 [cotreatment]
    *** source: 23913859-Fig-3b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (120 min)
    *** cells: AKR2B in BMLS
    *** unaffected by: CHX [pretreatment]
    *** unaffected by: ActinomycinD [pretreatment]
    *** source: 16288034-Fig-1b

no extras

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (times)
    *** cells: AKR2B in BMLS
    *** times: 0 1 3+ 6++ 9+ 12+ hr
    *** source: 19117990-Fig-2b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (4 hr)
    *** cells: AKR2B in BMLS
    *** source: 21454615-Fig-5a
  *** xAkt1[Ab]IP phos(S473)[phosAb] is increased irt Tgfb1 (120 min)
    *** cells: AKR2B<xAkt1> in BMLS
    *** source: 16288034-Fig-3
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (120 min)
    *** cells: AKR2B in BMLS
    *** source: 16288034-Fig-1c
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (120 min)
    *** cells: AKR2B in BMLS
    *** unaffected by: xAkt1 [addition]
    *** source: 16288034-Fig-3
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (120 min)
    *** cells: mEFs<Smad3~null><xAkt1><xSmad2(S467A)> in BMLS
    *** source: 16288034-Fig-3
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (120 min)
    *** cells: NIH3T3 in BMLS
    *** source: 16288034-Fig-1c
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (120 min)
    *** cells: SWISS3T3 in BMLS
    *** source: 16288034-Fig-1c
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Tgfb1 (times)
    *** cells: AKR2B in BMLS
    *** times: 0 15 30 60+ 90++ 120+++ min
    *** source: 16288034-Fig-1a

===============================
Does not work in all cell lines
===============================

  *** Akt1[phosAb] phos(T308)[phosAb] is unchanged irt Tgfb1 (30 min)
    *** cells: L17<xTgfbR1> in BMS
    *** source: 12034739-Fig-4a
  *** Akt1[phosAb] phos(S473)[phosAb] is unchanged irt Tgfb1 (120 min)
    *** cells: HELA in BMLS
    *** source: 16288034-Fig-1c
  *** Akt1[phosAb] phos(S473)[phosAb] is unchanged irt Tgfb1 (120 min)
    *** cells: MDCK in BMLS
    *** source: 16288034-Fig-1c
  *** Akt1[phosAb] phos(S473)[phosAb] is unchanged irt Tgfb1 (120 min)
    *** cells: MV1LU in BMLS
    *** source: 16288034-Fig-1c

===============================
Phosphorylation on unknown site
===============================

  *** Akt1[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (48 hr)
    *** cells: BXPC3<Smad4~null> in BMS
    *** source: 17638924-Fig-4b



