===============
Oligomerization
===============

  *** xTraf2[tAb] oligomerization[2TagPpt] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xSmad7 [addition]
    *** source: 17384642-Fig-S5

==============
Ubiquitination
==============

not Ciap2--

  *** rTraf2[35S] upshift[WBMS] is unchanged by (rCiap2 + rUbe2d2 + rUbe1 + rUbiq)
    *** cells: none
    *** source: 11907583-Fig-2a

not Xiap--

  *** rTraf2[35S] upshift[WBMS] is unchanged by (rXiap + rUbe2d2 + rUbe1 + rUbiq)
    *** cells: none
    *** source: 11907583-Fig-2a

==========================================
Traf2 features required for ubiquitination
==========================================

  *** xTraf2[tAb]IP ubiq[tUbAb] is detectable
    *** cells: HELA<xUbiq> in BMS
    *** partially inhibited by: xTraf2(C49A/H51A/C54A/C57A)"Ring-motif-mut" [substitution]
    *** partially inhibited by: xTraf2(C209A/C212A)"Zinc-finger-mut" [substitution]
    *** inhibited by: xTraf2(C49A/H51A/C209A/C212A) [substitution]
    *** source: 14713952-Fig-1b
  *** xTraf2[Ab]IP ubiq[UbAb] is increased itpo (xUbc13 + xUbiq)
    *** cells: HELA in BMS
    *** inhibited by: xTraf2(C34A)"E3-inactive" [addition]
    *** source: 12591926-Fig-1a

================
Deubiquitination
================

not Usp21--

  *** xTraf2[tAb] upshift[WBMS] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xUsp21 [addition]
    *** unaffected by: xUsp21(C221A)"CIA" [addition]
    *** source: 19910467-Fig-S2

================
Coprecipitations
================

Ciap1--

  *** xTraf2[tAb] copptby[WB] xCiap1[tAb] is detectable
    *** cells: HEK293 in BMS "treated with Ubiq Aldehyde"
    *** unaffected by: xCiap1(mnr)"E3-activity-mut" [substitution]
    *** source: 11907583-Fig-3b

Cd40--

  *** Traf2[Ab] snaggedby[WB] rCd40(216-277)[GST] is detectable
    *** cells: HELA in BMS
    *** inhibited by: rCd40((216-277)/T254A)"mT2/T3" [substitution]
    *** inhibited by: rCd40((216-277)/Q234A/E235A/Q263A/T254A)"mT2/T3/T6" [substitution]
    *** unaffected by: rCd40((216-277)/Q234A/E235A)"mT6" [substitution]
    *** unaffected by: rCd40((216-277)/Q234A/E235A/Q263A)"mT3/T6" [substitution]
    *** source: 16260598-Fig-1b

Cyld--

  *** Traf2[Ab] copptby[WB] xCyld[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 12917691-Fig-2d

Exsut--

  *** Traf2[Ab] copptby[WB] xEcsit[tAb] is undetectable
    *** cells: COS in BMS
    *** source: 10465784-Fig-2a
  *** Traf2[Ab] copptby[WB] xEcsit[tAb] is undetectable
    *** cells: HEK293 in BMS
    *** source: 10465784-Fig-2a

Map4k2--

  *** xTraf2[Ab] copptby[WB] xMap4k2[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 9712898-Fig-3a

Nik--

  *** xTraf2[tAb] copptby[WB] xNik[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 15084608-Fig-1a

Nod1--

  *** xTraf2[tAb] copptby[WB] xNod1[tAb] is undetectable
    *** cells: HEK293 in BMS
    *** source: 10329646(D)

Ripk1--

  *** xTraf2[tAb] copptby[WB] xRipk1[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 11479302-Fig-3

Tab2--

  *** xTraf2[tAb] copptby[WB] xTab2[tAb] is undetectable
    *** cells: HEK293 in BMS
    *** source: 14633987-Fig-4b
  *** xTraf2[tAb] copptby[WB] xTab2[tAb] is undetectable
    *** cells: HEK293<xIL1R1> in BMS
    *** source: 14633987-Fig-6a

Tab3--

  *** xTraf2[tAb] copptby[WB] xTab3[tAb] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xTab3(1-392)"Nterm" [substitution]
    *** unaffected by: xTab3(393-712)"Cterm" [substitution]
    *** source: 14633987-Fig-4b
  *** xTraf2[tAb] copptby[WB] xTab3[tAb] is detectable
    *** cells: HEK293<xIL1R1> in BMS
    *** source: 14633987-Fig-6a

Tak1--

  *** xTraf2[tAb] copptby[WB] xTak1[tAb] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xSmad7 [addition]
    *** source: 17384642-Fig-6f

Tbk1--

  *** xTraf2[tAb] copptby[WB] xTbk1(689-729)[GST] is detectable
    *** cells: HEK293<xTank> in BMS
    *** reqs: xTank [omission]
    *** unaffected by: xTank(1-190) [substitution]
    *** source: 10581243-Fig-7b
  *** xTraf2[tAb] copptby[WB] xTbk1[tAb] is detectable
    *** cells: HEK293<xTank> in BMS
    *** reqs: xTank [omission]
    *** unaffected by: xTank(1-190) [substitution]
    *** source: 10581243-Fig-7b

Tank--

  *** xTraf2[tAb] copptby[WB] xTank[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 10759890-Fig-4b
  *** xTraf2[tAb] copptby[WB] xTank[tAb] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: xIkke [addition]
    *** comment: coppt xTraf2 is upshifted itpo xIkke
    *** unaffected by: xIkke(K38A) [addition]
    *** comment: coppt xTraf2 is not upshifted itpo xIkke(K38A)
    *** source: 10759890-Fig-7a

TnfR1--

  *** xTraf2[tAb] copptby[WB] xTnfR1[Ab] is increased itpo xTradd
    *** cells: HEK293 in BMS
    *** unaffected by: xTraf1 [addition]
    *** source: 8565075-Fig-5a

Tradd--

  *** xTraf2[tAb] copptby[WB] xTradd[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 11479302-Fig-3

Traf5--

  *** xTraf2[tAb] copptby[WB] xTraf5[tAb] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xSmad7 [addition]
    *** source: 17384642-Fig-S5

Traf6--

  *** Traf2[Ab] copptby[WB] xTraf6[tAb] is detectable
    *** cells: HEK293<xCd40(Q234A/E235A)"mT6"> in BMS
    *** reqs: xCd40(Q234A/E235A)"mT6" [omission]
    *** source: 16260598-Fig-8a





















