=====================
Phosphorylation on Y9
=====================

  *** xPdpk1[phosAb] phos(Y9)[phosAb] is detectable
    *** cells: HEK293<xPdpk1><xSrc(mnr)"CA"> in BMS
    *** inhibited by: xPdpk1(Y9F) [substitution]
    *** source: 14585963-Fig-5d

=======================
Phosphorylation on S241
=======================

  *** Pdpk1[phosAb] phos(S241)[phosAb] is detectable
    *** cells: HC11 in BMLS "containing Ins"
    *** unaffected by: LY294002 [addition]
    *** unaffected by: PD98059 [addition]
    *** source: 16984645-Fig-4c

=============================
Phosphorylation on Y373/Y376
=============================

  *** xPdpk1[tAb]IP Yphos[pYAb] is detectable
    *** cells: HEK293<xPdpk1><xFak2> in BMS
    *** inhibited by: xPdpk1(Y9F) [substitution]
    *** partially inhibited by: xPdpk1(Y373F) [substitution]
    *** source: 14585963-Fig-4b

===========================
Phosphorylation on Tyrosine
===========================

requires Src--

  *** xPdpk1[tAb]IP Yphos[pYAb] is detectable
    *** cells: CHO<xAgtR1><xPdpk1><xFak2> in BMS
    *** inhibited by: xSrc(K296R/Y528F)"DN" [addition]
    *** source: 14585963-Fig-5b
  *** Pdpk1[Ab]IP Yphos[pYAb] is detectable
    *** cells: rVSMCs in BMS
    *** inhibited by: PP1 [pretreatment]
    *** source: 14585963-Fig-5a
  *** xPdpk1[tAb]IP Yphos[pYAb] is detectable
    *** cells: CHO<xAgtR1><xPdpk1><xFak2> in BMS
    *** inhibited by: PP1 [pretreatment]
    *** source: 14585963-Fig-5b

=====================
Total Phosphorylation
=====================

autophosphorylation

  *** rPdpk1[32P] phos[32P-ATP] is detectable
    *** cells: none
    *** inhibited by: rPdpk1(mnr)"KD" [substitution]
    *** source: 9768361-Fig-4

================
Coprecipitations
================

Akt1--

  *** Pdpk1[Ab] copptby[WB] Akt1[Ab] is detectable
    *** cells: DU145 in BMLS
    *** inhibited by: xIlk [addition]
    *** unaffected by: xIlk(S434A) [addition]
    *** source: 11313365-Fig-8a

not Cdkn1b--

  *** rPdpk1[Ab] boundby[WB] rCdkn1b[tAb] is undetectable
    *** cells: none
    *** source: 18662999-Fig-1d

Fak2--

  *** xPdpk1[tAb] copptby[WB] xFak2[tAb] is detectable
    *** cells: HEK293<xPdpk1><xFak2> in BMS
    *** inhibited by: xFak2(K457A) [substitution]
    *** source: 14585963-Fig-6c
  *** xPdpk1[tAb] copptby[WB] xFak2[tAb] is detectable
    *** cells: HEK293<xPdpk1><xFak2> in BMS
    *** inhibited by: xPdpk1(Y9F) [substitution]
    *** source: 14585963-Fig-6c

Freud1--

  *** rPdpk1[Ab] boundby[WB] rFreud1[tAb] is detectable
    *** cells: none
    *** source: 18662999-Fig-1d
  *** Pdpk1[Ab] copptby[WB] xFreud1[tAb] is detectable
    *** cells: HT1080 in BMLS
    *** unaffected by: Egf (10 min) [stim]
    *** unaffected by: AG1478 [addition]
    *** unaffected by: Igf1 (10 min) [stim]
    *** source: 18662999-Fig-5d
  *** xPdpk1[tAb] copptby[WB] xFreud1[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 18662999-Fig-1b
  *** xPdpk1[tAb] copptby[WB] xFreud1[tAb] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: LY294002 [addition]
    *** source: 18662999-Fig-5f

Ilk--

  *** Pdpk1[Ab] copptby[WB] Ilk[Ab] is detectable
    *** cells: DU145 in BMLS
    *** unaffected by: Serum (tnr) [stim]
    *** source: 11313365-Fig-8a

Ywhaq--

  *** xPdpk1[tAb] copptby[WB] xYwhaq[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 18662999-Fig-1b
















