=====================
relocation to nucleus
=====================

Cathepsins--
Ctsb--
Ctsh--

  *** Rela[Ab] locatedin(nucleus)[IHC] is increased irt PolyIC (75 min)
    *** cells: RPE1 in BMS
    *** does not req: Ctsa [RNAi]
    *** does not req: Ctsc [RNAi]
    *** does not req: Ctsd [RNAi]
    *** does not req: Ctse [RNAi]
    *** does not req: Ctsf [RNAi]
    *** does not req: Ctsk [RNAi]
    *** does not req: Ctso [RNAi]
    *** does not req: Ctss [RNAi]
    *** does not req: Ctsw [RNAi]
    *** does not req: Ctsz [RNAi]
    *** source: 22611194-Table-S1
  *** Rela[Ab] locatedin(nucleus)[IHC] is increased irt PolyIC (75 min)
    *** cells: RPE1 in BMS
    *** does not req: CtsL1 [RNAi]
    *** does not req: Lgmn [RNAi]
    *** reqs: Ctsb [RNAi]
    *** reqs: Ctsh [RNAi]
    *** source: 22611194-Fig-6a

not Pkr--

  *** Rela[Ab] locatedin(nucleus)[IHC] is increased irt PolyIC-transfection (3 hr)
    *** cells: mEFs in BMLS
    *** does not req: Pkr [KO]
    *** source: 11113181-Fig-4

not Tbk1--

  *** Rela[Ab] infraction(nuclear)[WB] is increased irt PolyIC (times)
    *** cells: mBMMs in BMS
    *** times: 0 30+ 60++ min
    *** does not req: Tbk1 or TnfR1 [DKO]
    *** source: 15210743-Fig-3a

not TnfR1--

  *** Rela[Ab] locatedin(nucleus)[IHC] is increased irt PolyIC (75 min)
    *** cells: RPE1 in BMS
    *** does not req: TnfR1 [RNAi]
    *** source: 22611194-Table-S1

TLR3--

  *** Rela[Ab] locatedin(nucleus)[IHC] is increased irt PolyIC (75 min)
    *** cells: RPE1 in BMS
    *** reqs: TLR3 [RNAi]
    *** comment: TLR3 is still required if PolyIC is complexed with Dotap
    *** source: 22611194-Fig-4b
  *** Rela[Ab] locatedin(nucleus)[IHC] is increased irt PolyIC (75 min)
    *** cells: RPE1 in BMS
    *** reqs: TLR3 [RNAi]
    *** source: 22611194-Fig-1a
  *** Rela[Ab] locatedin(nucleus)[IHC] is increased irt PolyIC (75 min)
    *** cells: RPE1 in BMS
    *** reqs: TLR3 [RNAi]
    *** source: 22611194-Fig-6a

Traf3--  ???

  *** Rela[Ab] infraction(nuclear)[WB] is increased irt PolyIC (times)
    *** cells: mBMMs in BMS
    *** times: 0 45+ 90++ min
    *** bkg reqs: Traf3 [KO]
    *** source: 16306936-Fig-2e

Unc93b1--

  *** Rela[Ab] locatedin(nucleus)[IHC] is increased irt PolyIC (75 min)
    *** cells: RPE1 in BMS
    *** reqs: Unc93b1 [RNAi]
    *** source: 22611194-Fig-3e

chemicals

  *** Rela[Ab] locatedin(nucleus)[IHC] is increased irt PolyIC (75 min)
    *** cells: hMDDCs in BMS
    *** inhibited by: Leupeptin [pretreatment]
    *** inhibited by: Z-FA-FMK [pretreatment]
    *** source: 22611194-Fig-2d
  *** Rela[Ab] locatedin(nucleus)[IHC] is increased irt PolyIC (75 min)
    *** cells: RPE1 in BMS
    *** inhibited by: ConcanamycinA [pretreatment]
    *** source: 22611194-Fig-4a
  *** Rela[Ab] locatedin(nucleus)[IHC] is increased irt PolyIC (75 min)
    *** cells: RPE1 in BMS
    *** inhibited by: Dynasore [pretreatment]
    *** comment: Dynasore is not inhibitory if PolyIC is complexed with Dotap
    *** source: 22611194-Fig-4b
  *** Rela[Ab] locatedin(nucleus)[IHC] is increased irt PolyIC (75 min)
    *** cells: RPE1 in BMS
    *** inhibited by: Leupeptin [pretreatment]
    *** inhibited by: Z-FA-FMK [pretreatment]
    *** source: 22611194-Fig-2b
  *** Rela[Ab] locatedin(nucleus)[IHC] is increased irt PolyIC (75 min)
    *** cells: RPE1 in BMS
    *** inhibited by: Leupeptin [pretreatment]
    *** source: 22611194-Fig-2a
  *** Rela[Ab] locatedin(nucleus)[IHC] is increased irt PolyIC (75 min)
    *** cells: RPE1 in BMS
    *** unaffected by: CHX [pretreatment]
    *** source: 22611194-Fig-3c

no extras

  *** Rela[Ab] locatedin(nucleus)[IHC] is increased irt PolyIC (times)
    *** cells: RPE1 in BMS
    *** times: 0+ 15+ 30+ 60++ 75++ 90++ min
    *** source: 22611194-Fig-S1f
  *** Rela[Ab] infraction(nuclear)[WB] is increased irt PolyIC (times)
    *** cells: mBMMs in BMS
    *** times: 0 15 30++ 60++ 90+ min
    *** source: 12354379-Fig-3a
  *** Rela[Ab] locatedin(nucleus)[IHC] is unchanged irt PolyIC (times)
    *** cells: U373<xCd14> in BMS
    *** times: 0 60 90 min
    *** source: 22065573-Fig-3b
  *** Rela[Ab] locatedin(nucleus)[IHC] is increased irt PolyIC-transfection (3 hr)
    *** cells: mEFs in BMLS
    *** source: 11113181-Fig-3


oh why do people do experiments like these?

  *** Rela[Ab] prot-exp[WB] is detectable-but unchanged irt PolyIC (times)
    *** cells: U373<xCd14> in BMS "nuclear extracts"
    *** times: 0+ 30+ 60+ 120+ min
    *** source: 22065573-Fig-7a
