===============
Kinase Activity
===============

Raf1 features

  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,Elk1)[32P-ATP] is increased irt PMA (5 min)
    *** cells: HEK293 in BMS
    *** enhanced by: xRaf1(S497A) [substitution]
    *** enhanced by: xRaf1(S499A) [substitution]
    *** enhanced by: xRaf1(T506A) [substitution]
    *** source: 11447113-Fig-1c
  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,Elk1)[32P-ATP] is increased irt PMA (5 min)
    *** cells: HEK293 in BMS
    *** partially inhibited by: xRaf1(T491A) [substitution]
    *** partially inhibited by: xRaf1(S494A) [substitution]
    *** inhibited by: xRaf1(T491A/S494A) [substitution]
    *** source: 11447113-Fig-1b
  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,Elk1)[32P-ATP] is increased irt PMA (5 min)
    *** cells: HEK293 in BMS
    *** partially inhibited by: xRaf1(Y341F) [substitution]
    *** partially inhibited by: xRaf1(S338A) [substitution]
    *** source: 11447113-Fig-3c
  *** xRaf1[tAb]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt PMA (1 hr)
    *** cells: COS in BMLS
    *** inhibited by: xRaf1(S338A) [substitution]
    *** inhibited by: xRaf1(Y341A) [substitution]
    *** unaffected by: xRaf1(S339A) [substitution]
    *** unaffected by: xRaf1(Y340A) [substitution]
    *** source: 10205168-Fig-4c
  *** xRaf1[tAb]IP IVKA(Mek1,Erk2)[32P-ATP] is increased irt PMA (10 min)
    *** cells: COS7 in BMLS
    *** source: 12738796-Fig-1c

Chemicals

  *** Raf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt PMA (5 min)
    *** cells: CTLL2 in BMS "IL2-deprived"
    *** unaffected by: Wortmannin [pretreatment]
    *** source: 7760801-Fig-9

no extras

  *** Raf1[Ab]IP IVKA(auto)[32P-ATP] is increased irt PMA (15 min)
    *** cells: hIL2RTCs in BMHIS "IL2-deprived for 12-14 hr"
    *** source: 1708096-Fig-4b
  *** Raf1[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt PMA (1 hr)
    *** cells: COS in BMS
    *** source: 10205168-Fig-4a
  *** xRaf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt PMA (times)
    *** cells: CHO in BMS
    *** times: 0 5+ 10++ 15+ 30+ 60+ min
    *** source: 8120027-Fig-1b
  *** Raf1[Ab]IP IVKA(Mek1)[32P-ATP] is unchanged irt PMA (5 min)
    *** cells: RAT1 in BMLS
    *** source: 9774335-Fig-3b
  *** Raf1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt PMA (10 min)
    *** cells: CTLL2 in BMS
    *** source: 1996324-Fig-1
  *** Raf1[Ab]IP phos(S259)[phosAb] is decreased irt PMA (times)
    *** cells: COS1 in BMS
    *** times: 0++ 2+ 5 min
    *** source: 11782426-Fig-1c
  *** Raf1[Ab]IP phos(S338)[phosAb] is increased irt PMA (1 hr)
    *** cells: COS in BMLS
    *** source: 10205168-Fig-4b
  *** Raf1[Ab]IP phos(S338)[phosAb] is increased irt PMA (times)
    *** cells: COS1 in BMS
    *** times: 0 2+ 5++ min
    *** source: 11782426-Fig-1c
  *** Raf1[Ab]IP phos[32Pi] is increased irt PMA (10 min)
    *** cells: CTLL2 in BMS
    *** source: 1996324-Fig-1
  *** Raf1[Ab]IP phos[32Pi] is unchanged irt PMA (15 min)
    *** cells: CTLL2 in BMS
    *** source: 1996324-Fig-2b
  *** xRaf1[tAb]IP IVKA(Mek1)(sitenr)[phosAb] is unchanged irt PMA (30 min)
    *** cells: COS1 in BMLS
    *** source: 16508002-Fig-2b

===============
Phosphorylation
===============

  *** xRaf1[tAb]IP phos(S338)[phosAb] is increased irt PMA (20 min)
    *** cells: COS in BMS
    *** inhibited by: xRaf1(S338A) [substitution]
    *** unaffected by: xRaf1(Y341A) [substitution]
    *** unaffected by: xRaf1(S339A) [substitution]
    *** unaffected by: xRaf1(Y340A) [substitution]
    *** source: 10205168-Fig-5a

