=======================
Complex kinase activity
=======================

Pras40--  ???

  *** Raptor[Ab]IP IVKA(Eif4ebp1)(T36/T45)[phosAb] is increased irt Ins (30 min)
    *** cells: NIH3T3 in BMLS
    *** reqs: Pras40 [RNAi]
    *** source: 17510057-Fig-6b
  *** Raptor[Ab]IP IVKA(Eif4ebp1)[32P-ATP] is increased irt Ins (30 min)
    *** cells: NIH3T3 in BMLS
    *** reqs: Pras40 [RNAi]
    *** source: 17510057-Fig-6b

not Raptor--

  *** Mtor[Ab]IP IVKA(Akt1)(S473)[phosAb] is increased irt Ins (15 min)
    *** cells: HELA in BMS
    *** does not req: Raptor [RNAi] no-bkg-control
    *** source: 17043309-Fig-4c

Rheb--  ???

  *** Raptor[Ab]IP IVKA(S6k1)(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293E in BMLS
    *** enhanced by: xRheb{GTPgS}[tAb]IP [addition]
    *** unaffected by: xRap2a{GTPgS}[tAb]IP [addition]
    *** source: 17386266-Fig-2b

Rictor--

  *** Mtor[Ab]IP IVKA(Akt1)(S473)[phosAb] is increased irt Ins (15 min)
    *** cells: HELA in BMS
    *** reqs: Rictor [RNAi] no-bkg-control
    *** source: 17043309-Fig-4c

Sin1--

  *** Mtor[Ab]IP IVKA(Akt1)(S473)[phosAb] is increased irt Ins (15 min)
    *** cells: HELA in BMS
    *** reqs: Sin1 [RNAi] no-bkg-control
    *** source: 17043309-Fig-4c

Tsc2--

  *** Raptor[Ab]IP IVKA(S6k1)(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: mEFs in BMLS
    *** reqs: Tsc2 [KO]
    *** source: 17386266-Fig-1e

no extras

  *** Raptor[Ab]IP IVKA(Pras40)(T246)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293E in BMLS
    *** source: 17386266-Fig-S1
  *** Raptor[Ab]IP IVKA(Eif4ebp1)(T37/T46)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293E in BMLS
    *** source: 17386266-Fig-2e
  *** Raptor[Ab]IP IVKA(S6k1)(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293E in BMLS
    *** source: 17386266-Fig-1c
  *** Raptor[Ab]IP IVKA(S6k1)(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HELA in BMLS
    *** source: 17386266-Fig-1c
  *** Raptor[Ab]IP IVKA(S6k1)(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: mEFs in BMLS
    *** source: 17386266-Fig-1c
  *** xRaptor[tAb]IP IVKA(S6k1)(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293E<xRaptor> in BMLS
    *** source: 17386266-Fig-1d


===========================
inhibition by bound Pras40
===========================

  *** Mtor[Ab]IP IVKA(S6k1)(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293E in BMLS
    *** comment: bkg is increased when ppt is washed in high salt concentrations
    *** source: 17386266-Fig-1b
  *** Raptor[Ab]IP IVKA(Eif4ebp1)(T37/T46)[phosAb] is increased irt Ins (20 min)
    *** cells: HEK293E in BMLS
    *** comment: inhibited by addition of rPras40 to IVKA reaction
    *** source: 17386266-Fig-4f
  *** Raptor[Ab]IP IVKA(S6k1)(T412)[phosAb] is increased irt Ins (20 min)
    *** cells: HEK293E in BMLS
    *** comment: inhibited by addition of rPras40 to IVKA reaction
    *** source: 17386266-Fig-4b
  *** Raptor[Ab]IP IVKA(S6k1)(T412)[phosAb] is increased irt Ins (20 min)
    *** cells: HEK293E in BMLS
    *** comment: inhibited by addition of rPras40 to IVKA reaction
    *** source: 17386266-Fig-4e
  *** xRaptor[Ab]IP IVKA(S6k1)(T412)[phosAb] is increased irt Ins (20 min)
    *** cells: HEK293E<xRaptor> in BMLS
    *** comment: bkg is increased when ppt is washed in high salt concentrations
    *** comment: increase in bkg is inhibited by addition of rPras40 to IVKA reaction
    *** source: 17386266-Fig-4a

  *** Raptor[Ab]IP IVKA(Eif4ebp1)(T36/T45)[phosAb] is increased irt Ins (30 min)
    *** cells: 3T3L1 in BMLS "differentiated into adipocytes"
    *** bkg inhibited by: xPras40 [addition]
    *** unaffected by: xPras40(F129A) [addition]
    *** source: 17510057-Fig-5b
  *** Raptor[Ab]IP IVKA(Eif4ebp1)[32P-ATP] is increased irt Ins (30 min)
    *** cells: 3T3L1 in BMLS "differentiated into adipocytes"
    *** bkg inhibited by: xPras40 [addition]
    *** unaffected by: xPras40(F129A) [addition]
    *** source: 17510057-Fig-5b

  *** xMtor[tAb]IP IVKA(auto)[32P-ATP] is detectable-but unchanged irt Ins (15 min)
    *** cells: CHO<xInsR> in BMLS
    *** inhibited by: xMtor(N2343K) [substitution]
    *** partially inhibited by: xMtor(S2035R) [substitution]
    *** source: 9334222-Fig-1a

  *** Mtor[phosAb] phos(S2448)[phosAb] is unchanged irt Ins (45 min)
    *** cells: HEK293 in BMLS
    *** source: 12777372-Fig-1a
  *** Mtor[phosAb] phos(S2481)[phosAb] is unchanged irt Ins (45 min)
    *** cells: HEK293 in BMLS
    *** source: 12777372-Fig-1a
  *** Mtor[Ab]IP phos(S2448)[phosAb] is increased irt Ins (5 min)
    *** cells: HEK293 in BMLS
    *** inhibited by: Wortmannin [addition] no-bkg-control
    *** unaffected by: Rapamycin [addition] no-bkg-control
    *** source: 10567225-Fig-2c
  *** Mtor[Ab]IP phos[32Pi] is increased irt Ins (times)
    *** cells: CHOIR800 in BMLS
    *** times: 0 5 10+ 15++ 30++ min
    *** source: 10567225-Fig-1a
  *** Mtor[Ab]IP phos[32Pi] is increased irt Ins (tnr)
    *** cells: 3T3L1 in BMLS
    *** source: 10567225-Fig-1a

  *** Raptor[Ab]IP IVKA(Eif4ebp1)[32P-ATP] is increased irt Ins (30 min)
    *** cells: 3T3L1 in BMLS "differentiated into adipocytes"
    *** source: 18372248-Fig-3a
  *** Raptor[Ab]IP IVKA(Pras40)[32P-ATP] is increased irt Ins (30 min)
    *** cells: 3T3L1 in BMLS "differentiated into adipocytes"
    *** source: 18372248-Fig-1a
  *** Raptor[Ab]IP IVKA(Pras40)[32P-ATP] is increased irt Ins (30 min)
    *** cells: 3T3L1 in BMLS "differentiated into adipocytes"
    *** comment: inhibited by disruption of Mtor/Raptor complex with Triton X100
    *** source: 18372248-Fig-3a

Check on this

  *** Mtor[Ab] snaggedby[WB] capped-mRNA[m7GTP] is increased irt Ins (times)
    *** cells: HEK293E in BMLS
    *** times: 0 2+ 5+ 10+ 20++ 30++ min
    *** source: 16286006-Fig-5b

  *** Mtor[Ab] copptby[WB] Eif3b[Ab] is increased irt Ins (times)
    *** cells: HEK293E in BMLS
    *** times: 0 2+ 5+ 10+ 20+ 30+ min
    *** source: 16286006-Fig-5a
  *** Mtor[Ab] copptby[WB] Eif3c[Ab] is increased irt Ins (30 min)
    *** cells: HEK293E in BMLS
    *** inhibited by: Rapamycin [addition]
    *** source: 16286006-Fig-3b
  *** xMtor[tAb] copptby[WB] Eif3c[Ab] is increased irt Ins (30 min)
    *** cells: HEK293E<xRaptor> in BMLS
    *** inhibited by: Rapamycin [addition]
    *** source: 16286006-Fig-3a

