=======================
Phosphorylation on S140
=======================

Atm--

  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (30 min)
    *** cells: GM9607<Atm~fmut> in BMS
    *** comment: band is less intense than that seen in GM637 (normal) cells
    *** source: 15175241-Fig-S1a
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (times)
    *** cells: HELA in BMS
    *** times: 0 2+ 15++ min
    *** partially reqs: Atm [RNAi]
    *** comment: no total protein controls
    *** source: 16427009-Fig-S2
  *** H2ax[phosAb] phos(S140)[IHC] is increased irt IR (1 hr)
    *** cells: hFibros<Atm~fmut><xAtm> in BMS
    *** partially reqs: xAtm [omission]
    *** source: 16327781-Fig-3a
  *** H2ax[phosAb] phos(S140)[IHC] is increased irt IR (times)
    *** cells: AT1ABR<Atm~fmut><xAtm> in BMS
    *** times: 0 30+ 360+ min
    *** unaffected by: xAtm(S367A) [substitution]
    *** unaffected by: xAtm(S1983A) [substitution]
    *** inhibited by: xAtm(S1981A) [substitution]
    *** source: 16858402-Fig-6a
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (times)
    *** cells: mEFs in BMS
    *** times: 0 5++ 15++ 30++ 60++ 120++ 240+ 480+ min
    *** reqs: Atm [KO]
    *** source: 11571274-Fig-2a
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (30 min)
    *** cells: mEFs in BMS
    *** reqs: Atm [KO]
    *** source: 11571274-Fig-2b
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (30 min)
    *** cells: mEFs<Atm~null><xAtm> in BMS
    *** reqs: xAtm [omission]
    *** source: 11571274-Fig-3b
  *** H2ax[phosAb] phos(S140)[IHC] is increased irt IR (30 min)
    *** cells: mEFs in BMS
    *** reqs: Atm [KO]
    *** source: 11571274-Fig-4

not Atr--

  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Atr [RNAi]
    *** source: 16327781-Fig-1b
  *** H2ax{phos(S140)}[phosAb] locatedin(DSBs)[IHC] is increased irt MicroLaser (10 min)
    *** cells: U2OS<xAtr> in BMS
    *** source: 16327781-Fig-6a

not Brca1--

  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (6 hr)
    *** cells: HELA in BMS "chromatin fraction"
    *** does not req: Brca1 [RNAi]
    *** source: 18001825-Fig-S6b
  *** H2ax[phosAb] phos(S140)[IHC] is detectable
    *** cells: U2OS in BMS "treated with MicroLaser for 1 hr"
    *** does not req: Brca1 [RNAi]
    *** source: 18001824-Fig-S8b

not Kdm6b-- Kdm6a-- Kdm4a--
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (tnr)
    *** cells: HEK293T in BMS
    *** unaffected by: xKdm6b [addition]
    *** unaffected by: xKdm4a [addition]
    *** unaffected by: xKdm4d [addition]
    *** source: 19783983-Fig-4d


Mdc1--  ???

  -------------- partially required ---------------------------
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (times)
    *** cells: mEFs in BMS
    *** times: 0 2+ 10++ 30++ 60+ min
    *** partially reqs: Mdc1 [KO]
    *** source: 16427009-Fig-4a
  *** H2ax[phosAb] phos(S140)[IHC] is increased irt IR (times)
    *** cells: mEFs in BMS
    *** times: 0 2+ 10++ 30++ 60+ min
    *** partially reqs: Mdc1 [KO]
    *** source: 16427009-Fig-S1
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (times)
    *** cells: HELA in BMS
    *** times: 0 2+ 15++ min
    *** partially reqs: Mdc1 [RNAi]
    *** source: 16427009-Fig-S2
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (times)
    *** cells: NotReported in BMS
    *** times: 0 1+ 2+ hr
    *** partially reqs: Mdc1 [RNAi]
    *** source: 12607005-Fig-5f
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (6 hr)
    *** cells: mEFs in BMS "chromatin fraction"
    *** partially reqs: Mdc1 [KO]
    *** source: 18001825-Fig-S6a
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** partially reqs: Mdc1 [KO]
    *** source: 16377563-Fig-S5a
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (times)
    *** cells: U2OS in BMS
    *** times: 0 10+++ 60+++ 120++ 240+ 480 720 min
    *** comment: phos goes away 120 min faster itao Mdc1 [RNAi]
    *** source: 16377563-Fig-6a
  -------------- not required ---------------------------
  *** H2ax[phosAb] phos(S140)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 30 min"
    *** does not req: Mdc1 [RNAi]
    *** source: 12607003-Fig-4c
  *** H2ax[phosAb] phos(S140)[IHC] is increased irt MicroLaser (10 min)
    *** cells: U2OS in BMS
    *** does not req: Mdc1 [RNAi]
    *** source: 15201865-Fig-3a
  *** H2ax[phosAb] phos(S140)[IHC] is detectable
    *** cells: U2OS in BMS "treated with MicroLaser for 30 min"
    *** does not req: Mdc1 [RNAi]
    *** source: 18001824-Fig-1f
  *** H2ax[phosAb] phos(S140)[IHC] is increased irt IR (tnr)
    *** cells: MCF7 in BMS
    *** does not req: Mdc1 [RNAi]
    *** source: 12551934-Fig-2b

not Mre11--

  *** H2ax{phos(S140)}[phosAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: D6809<Mre11~fmut><xMre11> in BMS "treated with IR for 6 hr"
    *** does not req: xMre11 [omission]
    *** unaffected by: xMre11(NLS) [substitution]
    *** source: 16478990-Fig-4d

not Nbs1--

  *** H2ax{phos(S140)}[phosAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS "treated with IR for 1 hr"
    *** does not req: xNbs1 [omission]
    *** unaffected by: xNbs1(1-734)"delC" [substitution]
    *** source: 15758953-Fig-2f

not Nbs1--

  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (times)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** times: 0 15+ 30++ 60++ min
    *** unaffected by: xNbs1 [omission]
    *** unaffected by: xNbs1(1-734)"delC" [substitution]
    *** source: 15758953-Fig-3a
  *** H2ax{phos(S140)}[phosAb] locatedin(subnuclear-bodies)[IHC] is increased irt IR (1 hr)
    *** cells: hFibros<Nbs1~fmut><xNbs1> in BMS
    *** does not req: xNbs1 [omission]
    *** source: 16327781-Fig-3c

not Prkdc--

  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (times)
    *** cells: HELA in BMS
    *** times: 0 2+ 15++ min
    *** does not req: Prkdc [RNAi]
    *** comment: no total protein controls
    *** source: 16427009-Fig-S2
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (times)
    *** cells: mEFs in BMS
    *** times: 0 5++ 15++ 30++ 60++ 120++ 240+ 480+ min
    *** does not req: Prkdc [KO]
    *** source: 11571274-Fig-2a
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (30 min)
    *** cells: mEFs in BMS
    *** does not req: Prkdc [KO]
    *** source: 11571274-Fig-2b
  *** H2ax[phosAb] phos(S140)[IHC] is increased irt IR (30 min)
    *** cells: mEFs in BMS
    *** does not req: Prkdc [KO]
    *** source: 11571274-Fig-4

not Rnf8--

  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (6 hr)
    *** cells: HELA in BMS "chromatin fraction"
    *** does not req: Rnf8 [RNAi]
    *** source: 18001825-Fig-S6b
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (6 hr)
    *** cells: HELA in BMS "chromatin fraction"
    *** does not req: Rnf8 [RNAi]
    *** source: 18001825-Fig-S6c
  *** xH2ax[phosAb] phos(S140)[phosAb] is increased irt IR (6 hr)
    *** cells: HELA<xH2ax> in BMS
    *** does not req: Rnf8 [RNAi]
    *** source: 18001825-Fig-6b
  *** H2ax{phos(S140)}[phosAb] locatedin(DSBs)[IHC] is detectable
    *** cells: U2OS in BMS "treated with MicroLaser for 30 min"
    *** does not req: Rnf8 [RNAi]
    *** source: 18001824-Fig-1e
  *** H2ax{phos(S140)}[phosAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 6 hr"
    *** does not req: Rnf8 [RNAi]
    *** source: 18001825-Fig-5a
  *** H2ax{phos(S140)}[phosAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 6 hr"
    *** does not req: Rnf8 [RNAi]
    *** source: 18001825-Fig-5b
  *** H2ax[phosAb] phos(S140)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 1 hr"
    *** does not req: Rnf8 [RNAi]
    *** source: 18006705-Fig-3e
  *** H2ax[phosAb] phos(S140)[IHC] is increased irt IR (1 hr)
    *** cells: HELA in BMS
    *** does not req: Rnf8 [RNAi]
    *** source: 18006705-Fig-S9a

not Ubc13--

  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (6 hr)
    *** cells: HELA in BMS "chromatin fraction"
    *** does not req: Ubc13 [RNAi]
    *** source: 18001825-Fig-S6c
  *** H2ax{phos(S140)}[phosAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 6 hr"
    *** does not req: Ubc13 [RNAi]
    *** source: 18001825-Fig-5a
  *** H2ax{phos(S140)}[phosAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 6 hr"
    *** does not req: Ubc13 [RNAi]
    *** source: 18001825-Fig-5b
  *** H2ax{phos(S140)}[phosAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 6 hr"
    *** does not req: Ubc13 [RNAi]
    *** source: 18001825-Fig-S5d
  *** H2ax[phosAb] phos(S140)[IHC] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Ubc13 [RNAi]
    *** source: 18006705-Fig-4b

H2ax features

  *** xH2ax[phosAb] phos(S140)[phosAb] is increased irt IR (6 hr)
    *** cells: mEFs<H2ax~null><xH2ax> in BMS
    *** unaffected by: xH2ax(K119R/K120R) [substitution]
    *** source: 18001825-Fig-S6e

chemicals

  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** inhibited by: KU55933 [pretreatment]
    *** source: 16327781-Fig-1b
  *** H2ax{phos(S140)}[phosAb] locatedin(subnuclear-bodies)[IHC] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** inhibited by: KU55933 [pretreatment]
    *** source: 16327781-Fig-S2
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (30 min)
    *** cells: mEFs in BMS
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 11571274-Fig-1c

no extras

  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (15 min)
    *** cells: 1070SK in BMS "acid-extracts"
    *** source: 15175241-Fig-1a
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (30 min)
    *** cells: GM637 in BMS
    *** source: 15175241-Fig-S1a
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (6 hr)
    *** cells: mEFs in BMS
    *** source: 18001825-Fig-6d
  *** xH2ax[phosAb] phos(S140)[phosAb] is increased irt IR (6 hr)
    *** cells: mEFs<H2ax~null><xH2ax> in BMS
    *** source: 18001825-Fig-6d
  *** H2ax{phos(S140)}[phosAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: mEFs in BMS "treated with IR for 6 hr"
    *** source: 18001825-Fig-S5c
  *** H2ax{phos(S140)}[phosAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: NotReported in BMS "treated with IR for 15 and 30 min"
    *** comment: colocates with Mdc1
    *** source: 12607005-Fig-2b
  *** H2ax{phos(S140)}[phosAb] locatedin(subnuclear-bodies)[IHC] is increased irt IR (times)
    *** cells: U2OS<xRnf8> in BMS
    *** times: 0 1++ 2++ 4++ 6++ 8++ 24+ hr
    *** source: 18001824-Fig-S4c
  *** H2ax{phos(S140)}[phosAb] locatedin(DSBs)[IHC] is increased irt Au197Ions (times)
    *** cells: AG1522D in BMS
    *** times: 0 1+ 12+ hr
    *** comment: colocates with Atm-phos(S2996)
    *** source: 21149446-Fig-9
  *** H2ax{phos(S140)}[phosAb] locatedin(DSBs)[IHC] is increased irt U238Ions (30 min)
    *** cells: GM8523HTERT<Atm~fmut> in BMS
    *** comment: colocates with Atm
    *** source: 21149446-Fig-10
  *** H2ax{phos(S140)}[phosAb] locatedin(DSBs)[IHC] is increased irt XenonIons (times)
    *** cells: AG1522D in BMS
    *** times: 0 5+ 60++ 1440+ min
    *** comment: colocates with Atm-phos(S367)
    *** source: 21149446-Fig-8
  *** H2ax[phosAb] phos(S140)[IHC] is increased irt IR (times)
    *** cells: 1070SK in BMS
    *** times: 0 5++ 60+ min
    *** source: 12556884-Fig-6a
  *** H2ax[phosAb] phos(S140)[IHC] is increased irt IR (times)
    *** cells: MCF7 in BMS
    *** times: 0 15+++++ 30+++++ 60++++ 120+++ 180++ 360+ min
    *** comment: time measurements are percent cells with foci
    *** source: 12551934-Fig-2a


=================================================
Unphosphorylated H2ax is distributed homogenously 
throughout nucleus regardless of DNA damage
=================================================

  *** H2ax[Ab] locatedin(nucleus)[IHC] is detectable
    *** cells: mEFs in BMS "treated with IR for 6 hr"
    *** comment: staining is homogeneous not speckled
    *** source: 18001825-Fig-S5a
  *** xH2ax[Ab] locatedin(nucleus)[IHC] is detectable
    *** cells: mEFs<H2ax~null><xH2ax> in BMS "treated with IR for 6 hr"
    *** comment: staining is homogeneous not speckled
    *** unaffected by: xH2ax(S140A) [substitution]
    *** source: 18001825-Fig-S5b

  *** H2ax{phos(S140)}[phosAb] boundto(Dab1-gene)[ChIP] is increased irt DSB-Induction (16 hr)
    *** cells: MCF7 in BMS
    *** source: 17486112-Fig-1e
  *** H2ax{phos(S140)}[phosAb] boundto(RN28S1-gene)[ChIP] is increased irt DSB-Induction (16 hr)
    *** cells: MCF7 in BMS
    *** source: 17486112-Fig-1c
  *** H2ax{phos(S140)}[phosAb] boundto(RN28S1-gene)[ChIP] is increased irt DSB-Induction (16 hr)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** does not req: xNbs1 [omission]
    *** source: 17486112-Fig-S3b


==============
Ubiquitination
==============

not Brca1--

  *** H2ax{phos(S140)}[phosAb] ubiq[WBMS] is detectable
    *** cells: HELA in BMS  "chromatin fraction" "treated with IR for 6 hr"
    *** does not req: Brca1 [RNAi]
    *** source: 18001825-Fig-S6b

Mdc1--

  *** H2ax{phos(S140)}[phosAb] ubiq[WBMS] is detectable
    *** cells: mEFs in BMS  "chromatin fraction" "treated with IR for 6 hr"
    *** partially reqs: Mdc1 [KO]
    *** source: 18001825-Fig-S6a

Rnf8--

  *** xH2ax[tAb]IP ubiq[WBMS] is increased irt IR (1 hr)
    *** cells: U2OS<xH2ax><xUbiq> in BMS
    *** reqs: Rnf8 [RNAi]
    *** source: 18001824-Fig-S9d
  *** xH2ax[tAb]IP ubiq[WBMS] is increased irt IR (6 hr)
    *** cells: HELA<xH2ax> in BMS
    *** reqs: Rnf8 [RNAi]
    *** source: 18001825-Fig-6b
  *** H2ax{phos(S140)}[phosAb] ubiq[WBMS] is detectable
    *** cells: HELA in BMS  "chromatin fraction" "treated with IR for 6 hr"
    *** partially reqs: Rnf8 [RNAi]
    *** source: 18001825-Fig-S6b
  *** H2ax{phos(S140)}[phosAb] ubiq[WBMS] is detectable
    *** cells: HELA in BMS  "chromatin fraction" "treated with IR for 6 hr"
    *** partially reqs: Rnf8 [RNAi]
    *** source: 18001825-Fig-S6c
  *** H2ax{phos(S140)}[phosAb] ubiq[WBMS] is detectable
    *** cells: HELA in BMS  "chromatin fraction" "treated with IR for 6 hr"
    *** partially reqs: Ubc13 [RNAi]
    *** source: 18001825-Fig-S6c
  *** H2ax{phos(S140)}[phosAb] ubiq[WBMS] is detectable
    *** cells: HELA<Rnf8~kd><xRnf8> in BMS "treated with IR for 6 hr"
    *** partially inhibited by: xRnf8(mnr)"delFHA" [substitution]
    *** partially inhibited by: xRnf8(mnr)"delRING" [substitution]
    *** source: 18001825-Fig-6f
  *** xH2ax{phos(S140)}[phosAb] ubiq[WBMS] is detectable
    *** cells: HELA<xH2ax> in BMS "treated with IR for 6 hr"
    *** partially  reqs: Rnf8 [RNAi]
    *** source: 18001825-Fig-6c
  *** xH2ax{phos(S140)}[phosAb]IP ubiq[UbAb] is detectable
    *** cells: HELA<xH2ax> in BMS "treated with IR for 6 hr"
    *** partially  reqs: Rnf8 [RNAi]
    *** source: 18001825-Fig-6c

reqs prior phos on S140

  *** xH2ax[Ab] ubiq[WBMS] is detectable
    *** cells: mEFs<H2ax~null><xH2ax> in BMS "treated with IR for 6 hr"
    *** inhibited by: xH2ax(S140A) [substitution]
    *** source: 18001825-Fig-6e


no extras

  *** H2ax{phos(S140)}[phosAb] ubiq[WBMS] is detectable
    *** cells: mEFs in BMS "treated with IR for 6 hr"
    *** source: 18001825-Fig-6d
  *** xH2ax[tAb] ubiq[WBMS] is increased irt IR (6 hr)
    *** cells: mEFs<H2ax~null><xH2ax> in BMS
    *** bkg inhibited by: xH2ax(K119R/K120R) [substitution]
    *** comment: unphosed xH2ax has a high bkg ubiq
    *** source: 18001825-Fig-S6e
  *** H2ax[phosAb] phos(S140)[phosAb] is increased irt IR (30 min)
    *** cells: mEFs in BMS
    *** source: 11571274-Fig-1b
























