=======================
Phosphorylation on T68
=======================

not Atr--

  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (times)
    *** cells: HELA in BMS
    *** times: 0 15+ 30+ 60+ min
    *** does not req: Atr [RNAi]
    *** source: 16431910-Fig-1b
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (1 hr)
    *** cells: hFibros<Atr~fmut> in BMS
    *** source: 16327781-Fig-1a
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Atr [RNAi]
    *** source: 16327781-Fig-1b

Atm--

  *** Chek2[Ab]IP phos(T68)[phosAb] is increased irt IR (2 hr)
    *** cells: hLymphoblasts in BMS
    *** reqs: Atm [fmut]
    *** source: 10973490-Fig-4c
  *** Chek2[phosAb] phos(T68)[phosAb] is unchanged irt IR (15 min)
    *** cells: AT3BI<Atm~fmut> in BMS
    *** source: 15234984-Fig-4a
  *** Chek2[phosAb] phos(T68)[phosAb] is unchanged irt IR (60 min)
    *** cells: AT3BI<Atm~fmut> in BMS
    *** source: 15234984-Fig-4b
  *** Chek2[phosAb] phos(T68)[phosAb] is unchanged irt IR (60 min)
    *** cells: AT3BI<Atm~fmut> in BMS
    *** source: 15234984-Fig-5
  *** Chek2[phosAb] phos(T68)[phosAb] is unchanged irt IR (1 hr)
    *** cells: hFibros<Atm~fmut> in BMS
    *** source: 16327781-Fig-1a
  *** Chek2[phosAb] phos(T68)[phosAb] is unchanged irt IR (times)
    *** cells: AT5BI<Atm~fmut> in BMS
    *** times: 0 10 60 min
    *** source: 14695167-Fig-2c
  ----------- contradiction --------------------------------------
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (10 min)
    *** cells: AT1ABR<Atm~fmut><xAtm> in BMS
    *** does not req: xAtm [omission]
    *** unaffected by: xAtm(S367A) [substitution]
    *** unaffected by: xAtm(S1983A) [substitution]
    *** unaffected by: xAtm(S1981A) [substitution]
    *** source: 16858402-Fig-5b
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (10 min)
    *** cells: hFibros in BMS
    *** does not req: Atm [RNAi]
    *** source: 14695167-Fig-2d

not Brca1--

  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Brca1 [RNAi]
    *** source: 19001859-Fig-3a
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (times)
    *** cells: hFibros in BMS
    *** times: 0 15+ 60+ min
    *** does not req: Brca1 [RNAi]
    *** source: 19001859-Fig-3b

not Claspin--

  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Claspin [RNAi]
    *** source: 16327781-Fig-S1b

Kdm6b--  ???
Ksm4a--  inhibition
Ksm4d--  inhibition

  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (tnr)
    *** cells: HEK293T in BMS
    *** unaffected by: xKdm6b [addition]
    *** inhibited by: xKdm4a [addition]
    *** inhibited by: xKdm4d [addition]
    *** source: 19783983-Fig-4e

not Mdc1--

  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Mdc1 [RNAi]
    *** source: 19001859-Fig-3a
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (times)
    *** cells: hFibros in BMS
    *** times: 0 15+ 60+ min
    *** does not req: Mdc1 [RNAi]
    *** source: 19001859-Fig-3b
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (30 min)
    *** cells: NotReported in BMS
    *** does not req: Mdc1 [RNAi]
    *** source: 12607003-Fig-5c
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Mdc1 [RNAi]
    *** source: 16377563-Fig-S5a
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (10 min)
    *** cells: hFibros in BMS
    *** does not req: Mdc1 [RNAi]
    *** source: 14695167-Fig-4a
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (10 min)
    *** cells: U2OS in BMS
    *** does not req: Mdc1 [RNAi]
    *** source: 18006705-Fig-S2b
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (10 min)
    *** cells: U2OS<Mdc1~kd><xMdc1> in BMS
    *** does not req: xMdc1 [omission]
    *** source: 18006705-Fig-S2b
  -------------- contradiction ---------------------------------
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (1 hr)
    *** cells: MCF7 in BMS
    *** partially reqs: Mdc1 [RNAi]
    *** source: 12551934-Fig-3b
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (times)
    *** cells: MCF7 in BMS
    *** times: 0 1+ 2++ 3+ hr
    *** comment: intensity at 2 hr is decreased itao Mdc1 [RNAi]
    *** source: 12551934-Fig-3a

Nbs1--  phos sites S278/S343

  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (15 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** reqs: xNbs1 [omission]
    *** inhibited by: xNbs1(1-652) [substitution]
    *** unaffected by: xNbs1(401-754) [substitution]
    *** partially inhibited by: xNbs1(S278A/S343A) [substitution]
    *** source: 15234984-Fig-4a
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (60 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** partially reqs: xNbs1 [omission]
    *** partially inhibited by: xNbs1(1-652) [substitution]
    *** unaffected by: xNbs1(401-754) [substitution]
    *** partially inhibited by: xNbs1(S278A/S343A) [substitution]
    *** source: 15234984-Fig-4b
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (60 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** partially reqs: xNbs1 [omission]
    *** partially inhibited by: xNbs1(1-652) [substitution]
    *** unaffected by: xNbs1(401-754) [substitution]
    *** partially inhibited by: xNbs1(S278A/S343A) [substitution]
    *** source: 15234984-Fig-5
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (times)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** times: 0 15+ 30++ 60++ min
    *** partially reqs: xNbs1 [omission]
    *** partially inhibited by: xNbs1(1-734)"delC" [substitution]
    *** source: 15758953-Fig-3a
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (15 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** reqs: xNbs1 [omission]
    *** inhibited by: xNbs1(1-652)"Nb652" [substitution]
    *** unaffected by: xNbs1(454-754)"NbFR5" [substitution]
    *** source: 16478990-Fig-3b
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (15 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** reqs: xNbs1 [omission]
    *** partially inhibited by: xNbs1(1-735)"dAtm" [substitution]
    *** unaffected by: xNbs1(454-754)"NbFR5" [substitution]
    *** inhibited by: xNbs1(454-735)"NbFR5sAtm" [substitution]
    *** source: 16478990-Fig-6b
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (times)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** times: 0 10+ 30+ 60+ 120+ min
    *** partially reqs: xNbs1 [omission]
    *** unaffected by: xNbs1(S343A) [substitution]
    *** source: 14695167-Fig-3a
  ---------------- contradiction ---------------------------------
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (10 min)
    *** cells: hFibros in BMS
    *** does not req: Nbs1 [RNAi]
    *** source: 14695167-Fig-4a



Mre11--

  *** Chek2[Ab]IP phos(T68)[phosAb] is increased irt IR (15 min)
    *** cells: D6809<Mre11~fmut><xMre11> in BMS
    *** partially reqs: xMre11 [omission]
    *** unaffected by: xMre11(NLS) [substitution]
    *** source: 16478990-Fig-4c

not Rnf8--

  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Rnf8 [RNAi]
    *** source: 18001824-Fig-S7d

Tip60--

  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (times)
    *** cells: HELAS3<xTip60> in BMS
    *** times: 0 30+ 120+ 240+ min
    *** inhibited by: xTip60(Y47A)"CD" [substitution]
    *** source: 19783983-Fig-S2b

not Topbp1--

  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Topbp1 [RNAi]
    *** source: 16327781-Fig-S1b

Tp53bp1--  ????

  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** partially reqs: Tp53bp1 [RNAi]
    *** comment: req enhanced by additional removal of Mdc1 and Brca1
    *** source: 19001859-Fig-3a
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (30 min)
    *** cells: NBS1LBI<Nbs1~fmut> in BMS
    *** partially reqs: Tp53bp1 [RNAi]
    *** source: 14695167-Fig-5a
  -------------------- contradiction -------------------------------
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (times)
    *** cells: hFibros in BMS
    *** times: 0 15+ 60+ min
    *** does not req: Tp53bp1 [RNAi]
    *** source: 19001859-Fig-3b

chemicals

  *** Chek2[Ab] phos[WBMS] is increased irt IR (times)
    *** cells: 70Z3 in BMS
    *** times: 0 1+ 2+ 3+ 4+ hr
    *** unaffected by: Thapsigargin [pretreatment]
    *** source: 14744935-Fig-S1b
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (45 min)
    *** cells: RAJI in BMS
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 16461339-Fig-1a
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** inhibited by: KU55933 [pretreatment]
    *** source: 16327781-Fig-1b
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (30 min)
    *** cells: U2OS in BMS
    *** unaffected by: Roscovitine [pretreatment]
    *** source: 16327781-Fig-7a

no extras

  *** Chek2[Ab]IP phos(T68)[phosAb] is increased irt IR (2 hr)
    *** cells: HEK293T in BMS
    *** source: 10973490-Fig-4a
  *** xChek2[tAb]IP phos(T68)[phosAb] is increased irt IR (2 hr)
    *** cells: HEK293T<xChek2> in BMS
    *** inhibited by: xChek2(T68A) [substitution]
    *** source: 10973490-Fig-4b
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (15 min)
    *** cells: GM637 in BMS
    *** source: 15234984-Fig-4a
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (60 min)
    *** cells: GM637 in BMS
    *** source: 15234984-Fig-4b
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (60 min)
    *** cells: GM637 in BMS
    *** source: 15234984-Fig-5
  *** Chek2[Ab]IP phos(T68)[phosAb] is increased irt IR (times)
    *** cells: HELA in BMS
    *** times: 0 10+ 30+ 60+ 120+ 240+ 480+ 720 min
    *** source: 16474843-Fig-1c
  *** Chek2[Ab]IP phos(T68)[phosAb] is increased irt IR (15 min)
    *** cells: GM637 in BMS
    *** source: 16478990-Fig-4c
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (15 min)
    *** cells: GM637 in BMS
    *** source: 16478990-Fig-3b
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (1 hr)
    *** cells: HELA in BMS
    *** source: 16327781-Fig-1a
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (30 min)
    *** cells: T24 in BMS
    *** comment: cells were synchronized at G0 by density arrest
    *** comment: unaffected by time after replating (12-32 hr)
    *** source: 16327781-Fig-6e
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt DSB-Induction (8 hr)
    *** cells: MCF7 in BMS
    *** source: 17486112-Fig-1b
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (tnr)
    *** cells: MCF7 in BMS
    *** source: 17486112-Fig-1b
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** source: 14695167-Fig-2c
  *** Chek2[phosAb] phos(T68)[phosAb] is increased irt IR (times)
    *** cells: HELA in BMS
    *** times: 0 10+ 60+ min
    *** source: 14695167-Fig-2b
  *** Chek2[phosAb] phos(T68)[IHC] is increased irt IR (times)
    *** cells: MCF7 in BMS
    *** times: 0 15+ 30+ 60+ 120+ 180+ 360+ min
    *** comment: time measurements are percent cells with foci not intensity
    *** comment: no controls showing location of total Chek2
    *** source: 12551934-Fig-2a

==========================
Phosphorylation on S33/S35
==========================

Atm--

  *** Chek2[phosAb] phos(S33/S35)[phosAb] is increased irt IR (10 min)
    *** cells: hFibros in BMS
    *** reqs: Atm [RNAi]
    *** source: 14695167-Fig-2d
  *** Chek2[phosAb] phos(S33/S35)[phosAb] is unchanged irt IR (times)
    *** cells: AT5BI<Atm~fmut> in BMS
    *** times: 0 10 60 min
    *** source: 14695167-Fig-2c

Mdc1--

  *** Chek2[phosAb] phos(S33/S35)[phosAb] is increased irt IR (10 min)
    *** cells: hFibros in BMS
    *** reqs: Mdc1 [RNAi]
    *** source: 14695167-Fig-4a

Nbs1--  S343 phos site

  *** Chek2[phosAb] phos(S33/S35)[phosAb] is increased irt IR (10 min)
    *** cells: hFibros in BMS
    *** reqs: Nbs1 [RNAi]
    *** source: 14695167-Fig-4a
  *** Chek2[phosAb] phos(S33/S35)[phosAb] is increased irt IR (10 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** reqs: xNbs1 [omission]
    *** inhibited by: xNbs1(S343A) [substitution]
    *** source: 14695167-Fig-3b

not Tp53bp1--

  *** Chek2[phosAb] phos(S33/S35)[phosAb] is increased irt IR (30 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** does not req: Tp53bp1 [RNAi]
    *** source: 14695167-Fig-5b
  *** Chek2[phosAb] phos(S33/S35)[phosAb] is increased irt IR (times)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** times: 0 10+ 30+ 60+ 120+ min
    *** reqs: xNbs1 [omission]
    *** inhibited by: xNbs1(S343A) [substitution]
    *** source: 14695167-Fig-3a

no extras

  *** Chek2[phosAb] phos(S33/S35)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** source: 14695167-Fig-2c
  *** Chek2[phosAb] phos(S33/S35)[phosAb] is increased irt IR (times)
    *** cells: HELA in BMS
    *** times: 0 10+ 60+ min
    *** source: 14695167-Fig-2b

=====================
Total Phosphorylation
=====================

  *** Chek2[Ab] phos[WBMS] is increased irt IR (tnr)
    *** cells: hFibros<Atm~fmut><xAtm> in BMS
    *** comment: reqs xAtm at 5-10 Gy but not at 50 Gy
    *** source: 10097108-Fig-3c
  *** Chek2[Ab] phos[WBMS-PPase] is increased irt IR (1 hr)
    *** cells: HELA in BMS
    *** source: 10097108-Fig-3a

===============
Kinase Activity
===============

Atm--

  *** Chek2[Ab]IP IVKA(Cdc25c)[32P-ATP] is unchanged irt IR (15 min)
    *** cells: AG04405<Atm~fmut> in BMS
    *** source: 10435585-Fig-5b
  *** Chek2[Ab]IP IVKA(Cdc25c)[32P-ATP] is increased irt IR (2 hr)
    *** cells: AT22IJET<Atm~fmut><xAtm> in BMS
    *** reqs: xAtm [omission]
    *** source: 9836640-Fig-4b
  *** Chek2[Ab]IP IVKA(Cdc25c)[32P-ATP] is increased irt IR (2 hr)
    *** cells: HEK293T in BMS
    *** source: 9836640-Fig-3e
  *** Chek2[Ab]IP IVKA(Cdc25c)[32P-ATP] is increased irt IR (tnr)
    *** cells: HEK293T in BMS
    *** source: 9836640-Fig-3d


  *** xChek2[tAb]IP IVKA(Cdc25c)[32P-ATP] is increased irt IR (1 hr)
    *** cells: mEFs<xChek2> in BMS
    *** inhibited by: xChek2(T68A) [substitution]
    *** inhibited by: xChek2(S19A/T26A/S28A/S33A/S35A/S50A/T68A) [substitution]
    *** source: 10973490-Fig-6b
  *** Chek2[Ab]IP IVKA(Cdc25c)[32P-ATP] is increased irt IR (15 min)
    *** cells: MRC5 in BMS
    *** source: 10435585-Fig-5b
  *** Chek2[Ab]IP IVKA(Cdc25c)[32P-ATP] is increased irt IR (1 hr)
    *** cells: HELA in BMS
    *** source: 10097108-Fig-3b

==================
Protein Expression
==================

  *** Chek2[Ab] prot-exp[WB] is decreased irt IR (times)
    *** cells: 70Z3 in BMS
    *** times: 0++ 1++ 2+ 3+ 4 hr
    *** source: 14744935-Fig-S1b
  *** Chek2[Ab]IP prot-exp[WB] is decreased irt IR (tnr)
    *** cells: HEK293T in BMS
    *** source: 9836640-Fig-3d

