H2ax--

  *** Brca1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (8 min)
    *** cells: mEFs in BMS
    *** reqs: H2ax [KO]
    *** source: 12792649-Fig-1c

Mdc1--  required for binding to chromatin near DSBs
        hard to tell whether this is a change in location
        or decreased in intensity

  *** Brca1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (4 hr)
    *** cells: U2OS<Mdc1~kd><xMdc1> in BMS
    *** reqs: xMdc1 [omission]
    *** unaffected by: xMdc1(del(196-506)) [substitution] 
    *** source: 18583988-Fig-S4a
  *** Brca1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: U2OS in BMS "treated with IR for 2 hr"
    *** reqs: Mdc1 [RNAi]
    *** source: 12607005-Fig-2c
  *** Brca1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS in BMS
    *** reqs: Mdc1 [RNAi]
    *** source: 16618811-Fig-6a
  *** Brca1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS in BMS
    *** partially reqs: Mdc1 [RNAi]
    *** source: 16618811-Fig-6b
  *** Brca1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS in BMS
    *** partially reqs: Mdc1 [RNAi]
    *** source: 16618811-Fig-6a
  *** Brca1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: mEFs in BMS "treated with IR for 6 hr"
    *** reqs: Mdc1 [KO]
    *** source: 18001825-Fig-S4d
  *** Brca1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: mEFs<Mdc1~null><xMdc1> in BMS "treated with IR for tnr"
    *** reqs: xMdc1 [omission]
    *** source: 16427009-Fig-S8
  *** Brca1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: mEFs in BMS "treated with IR for tnr"
    *** reqs: Mdc1 [KO]
    *** source: 18678890-Fig-4a
  ------- reqs Mdc1 sites phosed by Atm ---------------------------
  *** Brca1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: U2OS<Mdc1~kd><xMdc1> in BMS "treated with IR for tnr"
    *** reqs: xMdc1 [omission]
    *** inhibited by: xMdc1(T699A/T719A/T752A/T765A) [substitution]
    *** source: 18006705-Fig-S11a
  *** Brca1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: mEFs<Mdc1~null><xMdc1> in BMS "treated with IR for 6 hr"
    *** inhibited by: xMdc1(del(698-768)) [substitution]
    *** source: 18001825-Fig-4b
  *** Brca1[tAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: mEFs<Mdc1~null><xMdc1> in BMS "treated with IR for tnr"
    *** unaffected by: xMdc1(del(200-420))"dSDTD" [substitution]
    *** inhibited by: xMdc1(del(698-768))"dTQXF" [substitution]
    *** source: 18678890-Fig-4b

Rnf8--

  *** Brca1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 6 hr"
    *** reqs: Rnf8 [RNAi]
    *** source: 18001825-Fig-4a
  *** Brca1[Ab] locatedin(DSBs)[IHC] is detectable
    *** cells: U2OS in BMS "treated with MicroLaser for 1 hr"
    *** partially reqs: Rnf8 [RNAi]
    *** source: 18001824-Fig-4a
  *** Brca1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 6 hr"
    *** reqs: Rnf8 [RNAi]
    *** source: 18001825-Fig-S4a
  *** Brca1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA<Rnf8~kd><xRnf8> in BMS "treated with IR for 6 hr"
    *** inhibited by: xRnf8(mnr)"delFHA" [substitution]
    *** inhibited by: xRnf8(mnr)"delRING" [substitution]
    *** source: 18001825-Fig-4c
  *** Brca1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA<Rnf8~kd><xRnf8> in BMS "treated with IR for 6 hr"
    *** inhibited by: xRnf8(mnr)"delFHA" [substitution]
    *** inhibited by: xRnf8(mnr)"delRING" [substitution]
    *** source: 18001825-Fig-S4f
  *** Brca1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 1 hr"
    *** reqs: Rnf8 [RNAi]
    *** source: 18006705-Fig-3e


not Tp53bp1--

  *** Brca1[Ab] locatedin(DSBs)[IHC] is detectable
    *** cells: U2OS in BMS "treated with MicroLaser for 30 min"
    *** does not req: Tp53bp1 [RNAi]
    *** source: 18001824-Fig-S5

Ubc13--

  *** Brca1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 6 hr"
    *** reqs: Ubc13 [RNAi]
    *** source: 18001825-Fig-S5d

Effect of Cell Cycle

  *** Brca1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS in BMS
    *** comment: intensity drops in cells not expressing CyclinA
    *** source: 16618811-Fig-6c

timing

  *** xBrca1[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (14 min)
    *** cells: U2OS<xBrca1> in BMS
    *** comment: increase was monitored continuously during MicroLaser
    *** comment: increase was slower than xNbs1 xMdc1 xRnf8 in same system
    *** comment: increase was similar to  xTp53bp1 in same system
    *** comment: increase was faster than xAtr in same system
    *** source: 18001824-Fig-3b


chemicals

  *** Brca1[Ab] locatedin(DSBs)[IHC] is undetectable
    *** cells: U2OS in BMS "treated with MG132 for 90 min followed by MicroLaser for 30 min"
    *** comment: does not colocalize with H2ax{phos(S140)}[phosAb]
    *** source: 18001824-Fig-3c


no extras

  *** Brca1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (4 hr)
    *** cells: U2OS in BMS
    *** source: 18583988-Fig-S4a
  *** Brca1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (times)
    *** cells: MCF7 in BMS
    *** times: 0 15+ 30+ 60++ 120+++ 180+++ 360+++ min
    *** comment: time measurements are percent cells with foci
    *** source: 12551934-Fig-2a
  *** Brca1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (times)
    *** cells: U2OS in BMS
    *** times: 2 4+ 8+ 12+ 15+ min
    *** source: 18001824-Fig-S4a
  *** xBrca1[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (times)
    *** cells: U2OS<xMdc1> in BMS
    *** times: 0 30 60+ 120++ 360+++ sec
    *** source: 18001824-Fig-3a
