====================
DNA-binding activity
====================

Fak2--

  *** Stat5s[RE] oligo-binding[EMSA] is increased irt IL2 (15 min)
    *** cells: BAFB03<xIL2Rb> in BMS "IL2-deprived"
    *** unaffected by: xFak2 [addition]
    *** unaffected by: xFak2(mnr)"DN" [addition]
    *** source: 9512511-Fig-4d

Gab2--

  *** Stat5b[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (10 min)
    *** cells: KIT225<xGab2> in BMS "starved"
    *** oligo: GAS 5-GTATTTCCCAGAA-3
    *** unaffected by: xGab2(Y614F) [addition]
    *** inhibited by: xGab2(S623A) [addition]
    *** source: 15356145-Fig-7b

Jak1--

  *** xStat5a[Ab] oligo-binding[EMSA] is increased irt IL2 (10 min)
    *** cells: U4A<Jak1~null><Jak3~null><xJak1><xJak3><xIL2Rb><xIL2Rg> in BMS "nuclear-extracts"
    *** inhibited by: xJak1(K907A)"KD" [substitution]
    *** inhibited by: xJak1(Y1033F/Y1034F)"FF" [substitution]
    *** inhibited by: xJak1(Y1033F)"FY" [substitution]
    *** unaffected by: xJak1(Y1034F)"YF" [substitution]
    *** source: 9382798-Fig-7a
  *** xStat5a[Ab] oligo-binding[EMSA] is increased irt IL2 (10 min)
    *** cells: U4A<Jak1~null><Jak3~null><xJak1><xJak3><xIL2Rb><xIL2Rg> in BMS "nuclear-extracts"
    *** reqs: xJak1 [omission]
    *** source: 9382798-Fig-6

Jak3--

  *** xStat5a[Ab] oligo-binding[EMSA] is increased irt IL2 (10 min)
    *** cells: U4A<Jak1~null><Jak3~null><xJak1><xJak3><xIL2Rb><xIL2Rg> in BMS "nuclear-extracts"
    *** inhibited by: xJak3(K815A)"KD" [substitution]
    *** unaffected by: xJak3(Y975F/Y976F)"FF" [substitution]
    *** unaffected by: xJak3(Y975F)"FY" [substitution]
    *** unaffected by: xJak3(Y976F)"YF" [substitution]
    *** source: 9382798-Fig-7b
  *** xStat5a[Ab] oligo-binding[EMSA] is increased irt IL2 (10 min)
    *** cells: U4A<Jak1~null><Jak3~null><xJak1><xJak3><xIL2Rb><xIL2Rg> in BMS "nuclear-extracts"
    *** reqs: xJak3 [omission]
    *** source: 9382798-Fig-6

IL2Rb--

  *** xStat5a[Ab] oligo-binding[EMSA] is increased irt IL2 (10 min)
    *** cells: U4A<Jak1~null><Jak3~null><xJak1><xJak3><xIL2Rb><xIL2Rg> in BMS "nuclear-extracts"
    *** partially reqs: xIL2Rb [omission]
    *** source: 9382798-Fig-6

IL2Rg--

  *** xStat5a[Ab] oligo-binding[EMSA] is increased irt IL2 (10 min)
    *** cells: U4A<Jak1~null><Jak3~null><xJak1><xJak3><xIL2Rb><xIL2Rg> in BMS "nuclear-extracts"
    *** reqs: xIL2Rg [omission]
    *** source: 9382798-Fig-6

Shp2--

  *** Stat5s[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (5 min)
    *** cells: MYLA2059 in BMS
    *** oligo: "beta-casein-promo" 5-AGCTTAGATTTCTAGGAATTCAAATCA-3
    *** unaffected by: xShp2 [addition]
    *** unaffected by: Shp2(C459S)"CIA" [addition]
    *** source: 12543077-Fig-3b

Chemicals--

  *** Stat5s[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (times)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** times: 0 3+ 5++ 10++ 15++ min
    *** unaffected by: H7 [pretreatment]
    *** source: 9305919-Fig-7b

No extras--

  *** Stat5s[Ab] boundto(IL2Ra-gene)[ChIP] is increased irt IL2 (4 hr)
    *** cells: mSpCs in BMS "pretreated with anti-Cd3 for 72 hr"
    *** source: 14660657-Fig-3
  *** Stat5s[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (1 hr)
    *** cells: mSpCs in BMS "pretreated with anti-Cd3 for 72 hr"
    *** source: 14660657-Fig-2b
  *** Stat5s[Ab] oligo-binding[EMSA] is increased irt IL2 (times)
    *** cells: NKL in BMS "IL2-deprived"
    *** times: 0 10+ 20+ min
    *** source: 12372421-Fig-4b
  *** Stat5b[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "starved"
    *** oligo: GAS 5-GTATTTCCCAGAA-3
    *** source: 15356145-Fig-7b
  *** Stat5s[Ab] oligo-binding[EMSA] is increased irt IL2 (7 min)
    *** cells: NB2 in BMS "nuclear-extracts"
    *** oligo: GAS 5-GCCTGATTTCCCCGAAATGACGG-3
    *** source: 7479881-Fig-1b
  *** Stat5s[Ab] oligo-binding[EMSA] is increased irt IL2 (7 min)
    *** cells: YT in BMS "nuclear-extracts"
    *** oligo: GAS 5-GCCTGATTTCCCCGAAATGACGG-3
    *** source: 7479881-Fig-2b
  *** Stat5s[Ab] oligo-binding[EMSA] is increased irt IL2 (10 min)
    *** cells: ED40515<xIL2Rg> in BMS
    *** oligo: GAS 5-GCCTGATTTCCCCGAAATGATGA-3
    *** source: 10421845-Fig-2d
  *** Stat5s[Ab] oligo-binding[EMSA] is increased irt IL2 (15 min)
    *** cells: hPHAblasts in BMS
    *** oligo: "GRR" 5-ACGATGTTTCAAGGATTTGAGATGTATTTCCCAGAAAAG-3
    *** source: 7568001-Fig-3a
  *** Stat5s[Ab] oligo-binding[EMSA] is unchanged irt IL2 (10 min)
    *** cells: NKL in BMS "IL2-deprived"
    *** source: 7544001-Fig-4b
  *** Stat5s[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (10 min)
    *** cells: hIL2RTCs in BMS "IL2-deprived"
    *** oligo: "Siem67" 5-GTCGACATTTCCCGTAAATC-3
    *** source: 9305919-Fig-6c
  *** Stat5s[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** oligo: GAS 5-GTATTTCCCAGAAAAGGAAC-3
    *** source: 10602027-Fig-4
  *** Stat5s[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (times)
    *** cells: hIL2RTCs in BMS "IL2-deprived"
    *** times: 0 1+ 3++ 5++ 10+++ 15++ 30+ 60+ min
    *** oligo: "Siem67" 5-GTCGACATTTCCCGTAAATC-3
    *** source: 9305919-Fig-1a
  *** Stat5a[Ab] oligo-binding[EMSA] is unchanged irt IL2 (30 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** oligo: "IL2Ra-GASd/EBSd" 5-TTTCTTCTAGGAAGTACC-3
    *** source: 8943338-Fig-4a
  *** Stat5a[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (30 min)
    *** cells: hPHAblasts in BMS "IL2-deprived"
    *** oligo: "FcgRI-GAS" 5-GTATTTCCCAGAAAAGGAAC-3
    *** source: 8943338-Fig-5
  *** Stat5a[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (30 min)
    *** cells: hPHAblasts in BMS "IL2-deprived"
    *** oligo: "IL2Ra-GASd/EBSd" 5-TTTCTTCTAGGAAGTACC-3
    *** source: 8943338-Fig-5
  *** Stat5b[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (30 min)
    *** cells: hPHAblasts in BMS "IL2-deprived"
    *** oligo: "FcgRI-GAS" 5-GTATTTCCCAGAAAAGGAAC-3
    *** source: 8943338-Fig-5
  *** Stat5b[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (30 min)
    *** cells: hPHAblasts in BMS "IL2-deprived"
    *** oligo: "IL2Ra-GASd/EBSd" 5-TTTCTTCTAGGAAGTACC-3
    *** source: 8943338-Fig-5
  *** Stat5s[Ab] oligo-binding[EMSA] is increased irt IL2 (30 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** oligo: "IL2Ra-GASd/EBSd" 5-TTTCTTCTAGGAAGTACC-3
    *** source: 8943338-Fig-4a
  *** Stat5s[Ab] oligo-binding[EMSA] is increased irt IL2 (30 min)
    *** cells: mSpTCs in BMS "IL2-deprived"
    *** comment: unaffected by preactivation with IL2, anti-Cd3e, anti-Cd28 for 48 hr
    *** source: 16757175-Fig-2b

========================
Tyrosine Phosphorylation
========================

Cish--

  *** xStat5a[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: HEK293T<xIL2Rb(del(312-383))"delA"> in BMS
    *** unaffected by: xCish [addition]
    *** unaffected by: xCish(R107K) [addition]
    *** source: 10514520-Fig-7a
  *** xStat5a[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: HEK293T<xIL2Rb> in BMS
    *** inhibited by: xCish [addition]
    *** unaffected by: xCish(R107K) [addition]
    *** source: 10514520-Fig-7a
  *** Stat5b[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: BAF3<IL2Rb~null><xIL2Rb> in BMS
    *** unaffected by: xCish [addition]
    *** source: 10373548-Fig-4b
  *** Stat5s[Ab]IP Yphos[pYAb] is increased irt IL2 (times)
    *** cells: BAF3<IL2Rb~null><xIL2Rb> in BMS
    *** times: 0 5+ 10++ min
    *** partially inhibited by: xCish [addition]
    *** source: 10514520-Fig-7b

IL2Rb--

  *** xStat5a[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: HEK293T<xIL2Rb> in BMS
    *** unaffected by: xIL2Rb(del(312-383))"delA" [substitution]
    *** source: 10514520-Fig-7a

Jak3--

  *** Stat5a[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: NIH3T3<xIL2Ra><xIL2Rb><xIL2Rg><Jak3~null><Lck~null><xJak3> in BMS
    *** reqs: xJak3 [omission]
    *** source: 10825200-Fig-4e

Shp2--

  *** Stat5s[phosAb] phos(Y694)[phosAb] is increased irt IL2 (5 min)
    *** cells: MYLA2059 in BMS
    *** unaffected by: xShp2 [addition]
    *** unaffected by: Shp2(C459S)"CIA" [addition]
    *** source: 12543077-Fig-3a

Socs2--

  *** Stat5b[Ab]IP Yphos[pYAb] is increased irt IL2 (times)
    *** cells: BAF3 in BMS "IL3-deprived"
    *** times: 0 30+ 60+ min
    *** unaffected by: xSocs2 [addition]
    *** source: 16199887-Fig-2e

Socs3--

  *** Stat5b[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: BAF3<IL2Rb~null><xIL2Rb> in BMS
    *** inhibited by: xSocs3 [addition]
    *** source: 10373548-Fig-4a
  *** Stat5b[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: BAF3<IL2Rb~null><xIL2Rb> in BMS
    *** inhibited by: xSocs3 [addition]
    *** source: 10373548-Fig-4b
  *** Stat5b[Ab]IP Yphos[pYAb] is increased irt IL2 (times)
    *** cells: BAF3 in BMS "IL3-deprived"
    *** times: 0 30+ 60+ min
    *** inhibited by: xSocs3 [addition]
    *** source: 16199887-Fig-2e
  *** Stat5s[phosAb] phos(sitenr)[phosAb] is increased irt IL2 (30 min)
    *** cells: BAF3<IL2Rb~null><xIL2Rb> in BMS
    *** inhibited by: xSocs3 [addition]
    *** inhibited by: xSocs3(Y204F/Y221F) [addition]
    *** source: 11331873-Fig-4a

Syk--

  *** Stat5a[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: NIH3T3<xIL2Ra><xIL2Rb><xIL2Rg><Jak3~null><Lck~null><xJak3> in BMS
    *** unaffected by: xSyk [addition]
    *** unaffected by: xSyk(mnr)"KD" [addition]
    *** source: 10825200-Fig-4e

Chemicals--

  *** Stat5s[phosAb] phos(Y694)[phosAb] is increased irt IL2 (10 min)
    *** cells: hThymicBlasts in BMS "IL2-deprived"
    *** unaffected by: Rapamycin [pretreatment] no-bkg-control
    *** unaffected by: LY294002 [pretreatment] no-bkg-control
    *** unaffected by: Wortmannin [pretreatment] no-bkg-control
    *** unaffected by: PD98059 [pretreatment] no-bkg-control
    *** source: 12681450-Fig-5d
  *** Stat5s[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: NK33 in BMLS
    *** unaffected by: PP2 [pretreatment]
    *** source: 10849428-Fig-7a
  *** Stat5a[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: YT in BMS
    *** unaffected by: CP118556 [pretreatment]    
    *** source: 10825200-Fig-2d
  *** Stat5a[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: YT in BMS
    *** inhibited by: Staurosporine [pretreatment] no-bkg-control
    *** source: 10825200-Fig-2e

No extras--

  *** Stat5s[phosAb] phos(Y694)[phosAb] is increased irt IL2 (times)
    *** cells: hThymicBlasts in BMS "IL2-deprived"
    *** times: 0 5++ 10++ 20++ 60+ min
    *** source: 12681450-Fig-3b
  *** Stat5s[Ab] Yphos[pYAb-ppt] is increased irt IL2 (7 min)
    *** cells: NB2 in BMS
    *** source: 7479881-Fig-1d
  *** Stat5s[Ab] Yphos[pYAb-ppt] is increased irt IL2 (7 min)
    *** cells: YT in BMS
    *** source: 7479881-Fig-2e
  *** Stat5s[Ab] Yphos[pYAb-ppt] is increased irt IL2 (15 min)
    *** cells: hPHAblasts in BMS
    *** source: 7568001-Fig-2a
  *** Stat5s[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: hPHAblasts in BMS
    *** source: 7568001-Fig-2b
  *** Stat5s[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: hPHAblasts in BMS
    *** source: 7568001-Fig-2d
  *** Stat5s[Ab]IP Yphos[pYAb] is increased irt IL2 (20 min)
    *** cells: CTLL2 in BMS "IL2-deprived"
    *** source: 7781605-Fig-3b
  *** Stat5s[phosAb] phos(DGYV)[phosAb] is increased irt IL2
    *** cells: mSpTCs in BMS "IL2-deprived"
    *** comment: unaffected by preactivation with IL2, anti-Cd3e, anti-Cd28 for 48 hr
    *** source: 16757175-Fig-2a
  *** Stat5s[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: KIT225 in BMLS "IL2-deprived"
    *** source: 7760829-Fig-4a
  *** Stat5s[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: KIT225 in BMLS "IL2-deprived"
    *** source: 7760829-Fig-4c
  *** Stat5a[Ab]IP Yphos[pYAb] is increased irt IL2 (20 min)
    *** cells: hPHAblasts in BMS
    *** comment: bkg is enhanced when Ifng was added to PHA during blasting
    *** source: 9454765-Fig-2b
  *** Stat5s[Ab] oligo-binding[EMSA] is increased irt IL2 (20 min)
    *** cells: hPHAblasts in BMS "nuclear-extracts"
    *** source: 9454765-Fig-2c
  *** Stat5s[phosAb] phos(Y694)[phosAb] is increased irt IL2 (20 min)
    *** cells: hPHAblasts in BMS
    *** comment: bkg is enhanced when Ifng was added to PHA during blasting
    *** source: 9454765-Fig-2a
  *** Stat5s[phosAb] phos(Y694)[phosAb] is increased irt IL2 (20 min)
    *** cells: hPHAblasts in BMS
    *** source: 9454765-Fig-2b
  *** Stat5s[phosAb] phos(Y694)[phosAb] is increased irt IL2 (20 min)
    *** cells: hPHAblasts in BMS
    *** source: 9454765-Fig-6a
  *** Stat5s[phosAb] phos(DGYV)[phosAb] is increased irt IL2 (15 min)
    *** cells: hPBTCs<Itgb2~pos><Cd8~pos><Cd56~neg><Cd4~neg><Cd20~neg> in BMS
    *** unaffected by: IL12 [cotreatment]
    *** source: 10201984-Fig-4a


======================
Serine Phosphorylation
======================

Chemicals--

  *** Stat5a[Ab]IP phos(SSite)[phosAb] is increased irt IL2 (10 min)
    *** cells: hThymicBlasts in BMS "IL2-deprived"
    *** unaffected by: Rapamycin [pretreatment] no-bkg-control
    *** unaffected by: LY294002 [pretreatment] no-bkg-control
    *** unaffected by: Wortmannin [pretreatment] no-bkg-control
    *** unaffected by: PD98059 [pretreatment] no-bkg-control
    *** source: 12681450-Fig-5c
  *** Stat5a[Ab]IP phos(SSite)[phosAb] is increased irt IL2 (times)
    *** cells: hThymicBlasts in BMS "IL2-deprived"
    *** times: 0 5+ 10++ 20++ 60++ min
    *** source: 12681450-Fig-3c


==================================
Phosphorylation at unreported site
==================================

  *** Stat5s[phosAb] phos(sitenr)[phosAb] is increased irt IL2 (times)
    *** cells: CTLL2 in BMS "IL2-deprived"
    *** times: 0 5+ 15+ 30+ min
    *** unaffected by: xDusp5 [addition]
    *** unaffected by: xDusp5(C263S)"CIA" [addition]
    *** source: 12435740-Fig-6a
  *** Stat5s[phosAb] phos(sitenr)[phosAb] is increased irt IL2 (times)
    *** cells: hPHAblasts in BMS "IL2-PHA-deprived"
    *** times: 0 10+ 30+ min
    *** source: 12435740-Fig-5a

=====================
Total Phosphorylation
=====================

  *** Stat5s[Ab] phos[WBMS] is increased irt IL2 (times)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** times: 0 45+ 260+ 1440+ min
    *** inhibited by: Rapamycin [pretreatment]
    *** source: 10373522-Fig-1c

===============================
Recruitment to IL2R complex ??
===============================

  *** Stat5b[Ab] snaggedby[WB] rIL2Rb(382-525){phos}[GST] is detectable
    *** cells: KIT225 in BMS "IL2-deprived"
    *** inhibited by: rIL2Rb(382-525) [substitution]
    *** comment: snagged Stat5b mobility is decreased irt IL2 (tnr)
    *** source: 10602027-Fig-6c

==============
Stat5 reporter
==============

  *** Stat5-reporter[CAT] is increased irt IL2 (18 hr)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** partially inhibited by: xRb1(mnr)"DN" [addition] no-bkg-control
    *** source: 9390691-Fig-2b
  *** Stat5-reporter[CAT] is increased irt IL2 (18 hr)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** unaffected by: LY294002 [cotreatment]
    *** source: 9390691-Fig-5b
  *** Stat5-reporter[CAT] is increased irt IL2 (18 hr)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** unaffected by: Rapamycin [pretreatment]
    *** source: 10373522-Fig-1c
  *** Stat5-reporter[CAT] is increased irt IL2 (18 hr)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** unaffected by: xCdkn1b [addition]
    *** source: 9390691-Fig-3d
  *** Stat5-reporter[CAT] is increased irt IL2 (18 hr)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** unaffected by: xPik3r?(mnr)"DN" [addition]
    *** source: 9390691-Fig-5a
  *** Stat5-reporter[CAT] is increased irt IL2 (18 hr)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** unaffected by: xStat5? [addition]
    *** inhibited by: xStat5?(mnr)"DN" [addition]
    *** source: 9390691-Fig-4d



