================
Coppt with IL2Rb
================

  *** Shc1[Ab] copptby[WB] IL2Rb[Ab] is increased irt IL2 (5 min)
    *** cells: CTLL20 in BMS "IL2-deprived"
    *** source: 8294403-Fig-2c

================================
Proteins required for Shc1 Yphos
================================

IL2Rb--

  *** Shc1[Ab] Yphos[pYAb-ppt] is increased irt IL2 (15 min)
    *** cells: 32D<IL2Rb~null><xIL2Rb> in BMS "IL3-deprived"
    *** reqs: xIL2Rb [omission]
    *** source: 8700888-Fig-3b
  *** Shc1[Ab] Yphos[pYAb-ppt] is increased irt IL2 (15 min)
    *** cells: 32D<IL2Rb~null><xIL2Rb> in BMS "IL3-deprived"
    *** inhibited by: xIL2Rb(Y364F/Y418F/Y536F)"FYYYFF" [substitution]
    *** unaffected by: xIL2Rb(Y418F/Y536F)"YYYYFF" [substitution]
    *** inhibited by: xIL2Rb(Y364F/Y381F/Y383F/Y387F)"FFFFYY" [substitution]
    *** source: 8700888-Fig-3c
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: BAFB03<xIL2Rb> in BMS "IL3-deprived"
    *** reqs: xIL2Rb [omission]
    *** inhibited by: xIL2Rb(del(266-323)) [substitution]
    *** inhibited by: xIL2Rb(del(312-383)) [substitution]
    *** unaffected by: xIL2Rb(Y335F/Y358F) [substitution]
    *** source: 8643566-Fig-2
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: 32D<IL2Rb~null><xIL2Rb(Y418F/Y536F)"YYYYFF"> in BMS "IL3-deprived"
    *** inhibited by: xIL2Rb(Y364F/Y381F/Y383F/Y387F)"FFFFYY" [substitution]
    *** inhibited by: xIL2Rb(Y364F/Y418F/Y536F)"FYYYFF" [substitution]
    *** source: 8700888-Fig-3d

not Socs3--

  *** Shc1[Ab]IP Yphos[pYAb] is increased irt IL2 (30 min)
    *** cells: BAF3<IL2Rb~null><xIL2Rb> in BMS
    *** unaffected by: xSocs3 [addition]
    *** unaffected by: xSocs3(Y204F/Y221F) [addition]
    *** source: 11331873-Fig-4a

=============
Shc1 Features
=============

  *** xShc1[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: CTLL20 in BMS "IL2-deprived"
    *** inhibited by: xShc1(233-471) [substitution]
    *** source: 8643566-Fig-4a

=========
Chemicals
=========

CNI1493--

  *** Shc1[Ab]IP Yphos[pYAb] is increased irt IL2 (10 min)
    *** cells: BAFB03<xIL2Rb> in BMS "IL3-deprived"
    *** unaffected by: CNI1493 [pretreatment]
    *** source: 1066828-Fig-8b
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt IL2 (10 min)
    *** cells: CT6 in BMS "IL2-deprived"
    *** unaffected by: CNI1493 [pretreatment]
    *** source: 1066828-Fig-8a

Wortmannin--

  *** Shc1[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: CTLL2 in BMS "IL2-deprived"
    *** unaffected by: Wortmannin [pretreatment]
    *** source: 7760801-Fig-6



=========
No Extras
=========

  *** Shc1[Ab] Yphos[pYAb-ppt] is increased irt IL2 (times)
    *** cells: CTLL2 in BMLS "IL2-deprived"
    *** times: 0 1+ 15+ min
    *** source: 8119884-Fig-1b
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: CTLL20 in BMS "IL2-deprived"
    *** source: 8294403-Fig-2a
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: CTLL20 in BMS "IL2-deprived"
    *** source: 8643566-Fig-4a
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: hPHAblasts in BMS "IL2-deprived"
    *** source: 8643566-Fig-1
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: NK33 in BMLS
    *** source: 10849428-Fig-3b
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: NK33 in BMLS
    *** source: 10849428-Fig-3c
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: NK33 in BMLS
    *** source: 10849428-Fig-3d
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: CTLL2 in BMLS "IL2-deprived"
    *** source: 8119884-Fig-3
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt IL2 (times)
    *** cells: CTLL2 in BMLS "IL2-deprived"
    *** times: 0 1+ 5+ 15++ 30+ min
    *** source: 8119884-Fig-2
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt IL2 (times)
    *** cells: CTLL2 in BMLS "IL2-deprived"
    *** times: 0 1+ 15+ min
    *** source: 8119884-Fig-1a
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt IL2 (times)
    *** cells: CTLL2 in BMS "IL2-deprived"
    *** times: 0 2+ 5+ 10+ 20+ min
    *** source: 7688728-Fig-1b
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt IL2 (times)
    *** cells: CTLL20 in BMS "IL2-deprived"
    *** times: 0 1+ 5++ 15++ 30++ min
    *** source: 8294403-Fig-1

===================================================
Increase in copptby Grb2 is evidence for Shc1 Yphos
===================================================

  *** Shc1[Ab] copptby[WB] Grb2[Ab] is increased irt IL2 (15 min)
    *** cells: CTLL2 in BMLS "IL2-deprived"
    *** source: 8119884-Fig-4
  *** Shc1[Ab] snaggedby[WB] rGrb2(mnr)"SH2"[GST] is increased irt IL2 (5 min)
    *** cells: CTLL20 in BMS "IL2-deprived"
    *** source: 8294403-Fig-3a
  *** Shc1[Ab] snaggedby[WB] rGrb2[GST] is increased irt IL2 (15 min)
    *** cells: CTLL2 in BMLS "IL2-deprived"
    *** unaffected by: rGrb2(mnr)"SH2-domain" [substitution]
    *** source: 8119884-Fig-5

