=============
DNA-Synthesis
=============

Cish--  ???

  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (24 hr)
    *** cells: BAF3<IL2Rb~null> in BMS
    *** unaffected by: xCish [addition]
    *** source: 10373548-Fig-5a

Gab2--  ???

  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (68 hr)
    *** cells: KIT225<xGab2> in BMS
    *** enhanced by: xGab2(Y614F) [substitution]
    *** unaffected by: xGab2(S623A) [substitution]
    *** source: 15356145-Fig-8

reqs IL2Rb--

  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (48 hr)
    *** cells: CTLL2 in BMS
    *** unaffected by: anti-IL2Rb [cotreatment]
    *** unaffected by: anti-IL2Rg [cotreatment]
    *** inhibited by: anti-IL2Rb + IL2Rg [cotreatment]
    *** source: 8266076-Fig-2a
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (28 hr)
    *** cells: BAFB03<xIL2Rb> in BMS
    *** unaffected by: xIL2Rb(1-404)"H-mutant" [substitution]
    *** unaffected by: xIL2Rb(del(338-409))"A-mutant" [substitution]
    *** inhibited by: xIL2Rb(1-281)"ST-mutant" [substitution]
    *** source: 2590941-Fig-5
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (28 hr)
    *** cells: BAFB03<xIL2Rb> in BMS "IL3-deprived"
    *** inhibited by: xIL2Rb(mnr)"del-kinase-domain" [addition]
    *** source: 7568005-Fig-2c
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (24 hr)
    *** cells: 32D<IL2Rb~null><xIL2Rb> in BMS "IL3-deprived"
    *** reqs: xIL2Rb [omission]
    *** inhibited by: xIL2Rb(Y364F/Y381F/Y383F/Y387F/Y418F/Y536F)"FFFFFF" [substitution]
    *** enhanced by: xIL2Rb(Y381F/Y383F/Y387F)"YFFFYY" [substitution]
    *** inhibited by: xIL2Rb(Y364F/Y418F/Y536F)"FYYYFF" [substitution]
    *** partially inhibited by: xIL2Rb(Y418F/Y536F)"YYYYFF" [substitution]
    *** partially inhibited by: xIL2Rb(Y364F/Y381F/Y383F/Y387F/Y536F)"FFFFYF" [substitution]
    *** partially inhibited by: xIL2Rb(Y364F/Y381F/Y383F/Y387F/Y418F)"FFFFFY" [substitution]
    *** partially inhibited by: xIL2Rb(Y364F/Y381F/Y383F/Y387F)"FFFFYY" [substitution]
    *** unaffected by: xIL2Rb(Y381F/Y383F/Y387F/Y418F/Y536F)"YFFFFF" [substitution]
    *** source: 8700888-Fig-1
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (24 hr)
    *** cells: BAF3<xIL2Rb> in BMS "IL3-deprived"
    *** inhibited by: xIL2Rb(Y364F/Y381F/Y383F/Y387F/Y418F/Y536F)"FFFFFF" [substitution]
    *** source: 7665592-Fig-1b
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (24 hr)
    *** cells: BAF3<xIL2Rb> in BMS "IL3-deprived"
    *** inhibited by: xIL2Rb(Y364F/Y381F/Y383F/Y387F/Y418F/Y536F)"FFFFFF" [substitution]
    *** source: 7665592-Fig-2
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (24 hr)
    *** cells: BAF3<xIL2Rb> in BMS "IL3-deprived"
    *** inhibited by: xIL2Rb(Y364F/Y381F/Y383F/Y387F/Y418F/Y536F)"YF" [substitution]
    *** unaffected by: xIL2Rb(Y364F)"Y1F" [substitution]
    *** unaffected by: xIL2Rb(Y364F/Y418F/Y536F)"Y234F" [substitution]
    *** partially inhibited by: xIL2Rb(Y418F)"Y5F"  [substitution]
    *** partially inhibited by: xIL2Rb(Y536F)"Y6F" [substitution]
    *** unaffected by: xIL2Rb(Y364F/Y381F/Y383F/Y387F)"YF56Y" [substitution]
    *** inhibited by: xIL2Rb(Y418F/Y536F)"Y56F" [substitution]
    *** source: 7665592-Fig-3
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (28 hr)
    *** cells: BAF3<IL2Rb~null><xIL2Rb> in BMS
    *** reqs: xIL2Rb [omission]
    *** inhibited by: xIL2Rb(del(266-323)) [substitution]
    *** source: 8376945-Fig-3a
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (28 hr)
    *** cells: BAFB03<IL2Rb~null><xIL2Rb> in BMS
    *** unaffected by: xIL2Rb(1-379)"H4" [substitution]
    *** partially inhibited by: xIL2Rb(1-352)"SS1" [substitution]
    *** inhibited by: xIL2Rb(1-266)"ST14" [substitution]
    *** partially inhibited by: xIL2Rb(del(312-383))"A15" [substitution]
    *** inhibited by: xIL2Rb(del(266-323))"S25" [substitution]
    *** source: 7777534-Fig-4
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (28 hr)
    *** cells: BAFB03<xIL2Rb> in BMS
    *** reqs: xIL2Rb [omission]
    *** partially inhibited by: anti-IL2Ra [addition]
    *** partially inhibited by: anti-IL2Rb [addition]
    *** inhibited by: anti-IL2Ra + anti-IL2Rb [addition]
    *** source: 2590941-Table-2
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (78 hr)
    *** cells: ED515D in BMS
    *** partially inhibited by: anti-IL2Ra [cotreatment]
    *** unaffected by: anti-IL2Rb [cotreatment]
    *** inhibited by: anti-IL2Ra + anti-IL2Rb [cotreatment]
    *** source: 1940342-Fig-5
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (28 hr)
    *** cells: CTLL2 in BMS
    *** unaffected by: anti-IL2Ra + anti-IL2Rb [addition]
    *** source: 2590941-Table-2

reqs Jak3--

  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (28 hr)
    *** cells: BAFB03<xIL2Rb> in BMS "IL3-deprived"
    *** unaffected by: xJak3 [addition]
    *** inhibited by: xJak3(mnr)"del-kinase-domain" [addition]
    *** source: 7568005-Fig-2c
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (28 hr)
    *** cells: NIH3T3<xIL2Ra><xIL2Rb><xIL2Rg><Jak3~null><xJak3> in BMS
    *** reqs: xJak3 [omission]
    *** source: 7973659-Fig-3

Inhibited by Socs3--

  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (24 hr)
    *** cells: BAF3<IL2Rb~null> in BMS
    *** partially inhibited by: xSocs3 [addition]
    *** source: 10373548-Fig-5b

Stam2--  ???

  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (24 hr)
    *** cells: BAFB03<xIL2Rb><xIL2Rg> in BMS
    *** partially inhibited by: xStam2(del(209-256))"2DSH3" [substitution]
    *** source: 10899310-Fig-5b

reqs Stat5a-- or Stat5b--

  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (18 hr)
    *** cells: mSpCs in BMS "containing anti-Cd3"
    *** does not req: Stat5a [KO]
    *** does not req: Stat5b [KO]
    *** reqs: Stat5a or Stat5b [DKO]
    *** source: 10072077-Fig-3a

does not req Stat6--

  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (48 hr)
    *** cells: mLNCs in BMS
    *** does not req: Stat6 [KO]
    *** source: 8624821-Fig-3a

Chemicals

  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (18 hr)
    *** cells: CTLL2 in BMS "IL2-deprived"
    *** partially inhibited by: Wortmannin [pretreatment]
    *** inhibited by: Rapamycin [pretreatment]
    *** source: 7760801-Fig-2
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (36 hr)
    *** cells: TS1<xIL2Ra><xIL2Rb> in BMS
    *** partially inhibited by: Bisindolylmaleimide-I [cotreatment]
    *** source: 7737351-Fig-1
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (36 hr)
    *** cells: TS1<xIL2Rb> in BMS
    *** partially inhibited by: Bisindolylmaleimide-I [cotreatment]
    *** source: 7737351-Fig-1
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (96 hr)
    *** cells: hPBTCs<Itgb2~pos><Cd8~pos><Cd56~neg><Cd4~neg><Cd20~neg> in BMS
    *** inhibited by: SB203580 [pretreatment]
    *** source: 10201984-Fig-7a

No extras

  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (24 hr)
    *** cells: BAFB03<xIL2Rb><xIL2Rg> in BMS
    *** source: 10899310-Fig-5a
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (24 hr)
    *** cells: BAFB03<xIL2Rb><xIL2Rg> in BMS
    *** source: 10899310-Fig-5c
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (28 hr)
    *** cells: BAF3<IL2Rb~null><xBcl2><xIL2Rb> in BMS
    *** comment: inhibited by IL3-deprivation for more than 1 days
    *** source: 8376945-Fig-3b
  *** DNA-synthesis[3H-Thymidine] is increased irt IL2 (68 hr)
    *** cells: KIT225 in BMS
    *** source: 15356145-Fig-8

=======================================
Proliferation determined by Cell Number
=======================================

reqs Fak2--

  *** Proliferation[CellNumber] is increased irt IL2 (times)
    *** cells: BAFB03<xIL2Rb> in BMS "IL2-deprived"
    *** times: 0 1+ 2++ 3+++ days
    *** unaffected by: xFak2 [addition]
    *** partially inhibited by: xFak2(mnr)"DN" [addition]
    *** source: 9512511-Fig-4b
  *** Proliferation[CellNumber] is increased irt IL2 (times)
    *** cells: NIH3T3<xIL2Ra><xIL2Rb><xIL2Rg><Jak3~null><Lck~null><xJak3> in BMLS
    *** times: 0 2+ 4++ 6+++ days
    *** unaffected by: xFak2 [addition]
    *** inhibited by: xFak2(mnr)"DN" [addition]
    *** source: 9512511-Fig-4c

reqs Jak3--

  *** Proliferation[CellNumber] is increased irt IL2 (times)
    *** cells: NIH3T3<xIL2Ra><xIL2Rb><xIL2Rg><Jak3~null><Lck~null><xJak3> in BMLS
    *** times: 0 2+ 4++ 6+++ days
    *** reqs: xJak3 [omission]
    *** source: 9512511-Fig-4c
