===========
DNA-binding
===========

Effect of cotreatments

  *** Stat4[Ab] boundto(Ifng-gene)[ChIP] is increased irt IL12 (1 hr)
    *** cells: hPBTCs in BMS "preactivated with Cd3/Cd28"
    *** unaffected by: anti-Cd28 [cotreatment]
    *** source: 15522880-Fig-3a
  *** Stat4[Ab] boundto(Tbet-gene)[ChIP] is increased irt IL12 (times)
    *** cells: mSpCD4TCs in BMS "pretreated with anti-Cd3 + anti-Cd28 + anti-IL4 (48 hr)"
    *** times: 0 1++ 2+ 3++ 4++++ 5++++ 6+++ days
    *** source: 19409816-Fig-1c

Chemicals

  *** Stat4[Ab] boundto(Ifng-gene)[ChIP] is increased irt IL12 (1 hr)
    *** cells: hPBTCs in BMS "preactivated with Cd3/Cd28"
    *** unaffected by: CsA [pretreatment]
    *** partially inhibited by: Rapamycin [pretreatment]
    *** source: 15522880-Fig-3a

No extras

  *** Stat4[Ab] boundto(IL2Ra-gene)[ChIP] is increased irt IL12 (4 hr)
    *** cells: mSpCs in BMS "pretreated with anti-Cd3 for 72 hr"
    *** source: 14660657-Fig-3
  *** Stat4[Ab] boundto(IL2Ra-gene)[ChIP] is increased irt IL12 (times)
    *** cells: mSpCs in BMS "pretreated with anti-Cd3 for 72 hr"
    *** times: 0 2++ 4+ 6 24 hr
    *** source: 14660657-Fig-4
  *** Stat4[Ab] oligo-binding[EMSA] is increased irt IL12 (1 hr)
    *** cells: hTh1 in BMS
    *** source: 10037767-Fig-4
  *** Stat4[Ab] oligo-binding[EMSA] is increased irt IL12 (15 min)
    *** cells: hPHAblasts<Cd3e~neg> in BMS
    *** source: 7638186-Fig-3c
  *** Stat4[Ab] oligo-binding[EMSA] is increased irt IL12 (15 min)
    *** cells: KIT225 in BMS "nuclear-extracts"
    *** source: 12716907-Fig-3a
  *** Stat4[Ab] oligo-binding[EMSA] is increased irt IL12 (15 min)
    *** cells: mSpBlasts in BMS "nuclear-extracts"
    *** source: 9768751-Fig-4
  *** Stat4[Ab] oligo-binding[EMSA] is increased irt IL12 (15 min)
    *** cells: NK33 in BMS
    *** source: 10358173-Fig-5e
  *** Stat4[Ab] oligo-binding[EMSA] is increased irt IL12 (times)
    *** cells: NKL in BMS "IL2-deprived"
    *** times: 0 10+ 20+ min
    *** source: 12372421-Fig-4a
  *** Stat4[Ab] oligo-binding[EMSA] is increased irt IL12 (tnr)
    *** cells: 3F6 in BMS
    *** source: 7722452-Fig-3a
  *** Stat4[Ab] snaggedby[WB] oligo[agarose] is increased irt IL12 (tnr)
    *** cells: 3F6 in BMS "nuclear-extracts"
    *** source: 12716907-Fig-3b
  *** Stat4[Ab] snaggedby[WB] oligo[biotin] is increased irt IL12 (1 hr)
    *** cells: mSpCs in BMS "pretreated with anti-Cd3 for 72 hr" "cotreated with anti-IL2"
    *** source: 14660657-Fig-2b

Contradictions

  *** Stat4[Ab] oligo-binding[EMSA] is unchanged irt IL12 (1 hr)
    *** cells: hTh2 in BMS
    *** source: 10037767-Fig-4
  *** Stat4[Ab] oligo-binding[EMSA] is unchanged irt IL12 (15 min)
    *** cells: CB1 in BMS "nuclear-extracts"
    *** source: 9768751-Fig-4
  *** Stat4[Ab] oligo-binding[EMSA] is unchanged irt IL12 (15 min)
    *** cells: mSpDCs in BMS "nuclear-extracts"
    *** source: 9768751-Fig-4

=======================
Phosphorylation on S721
=======================

reqs Gadd45b--

  *** Stat4[Ab]IP phos(S721)[phosAb] is increased irt IL12 (tnr)
    *** cells: mSpTh1<xGadd45b> in BMS
    *** reqs: xGadd45b [omission]
    *** source: 12213961-Fig-1c

reqs Gadd45g--

  *** Stat4[Ab]IP phos(S721)[phosAb] is increased irt IL12 (tnr)
    *** cells: mSpTh1<xGadd45g> in BMS
    *** reqs: xGadd45g [omission]
    *** source: 12213961-Fig-1c

Stat4 features

  *** xStat4[Ab]IP phos(S721)[phosAb] is increased irt IL12 (45 min)
    *** cells: mSpCs<Stat4~null><xStat4> in BMS
    *** partially inhibited by: xStat4(Y693F)[phosAb] [substitution]
    *** inhibited by: xStat4(S721A)[phosAb] [substitution]
    *** source: 12213961-Fig-2b

No extras

  *** Stat4[Ab]IP phos(S721)[phosAb] is increased irt IL12 (times)
    *** cells: mSpBlasts in BMLS
    *** times: 0 5+ 45++ min
    *** source: 10961885-Fig-1a

=======================
Phosphorylation on Y693
=======================

not Gadd45b--

  *** Stat4[Ab]IP phos(Y693)[phosAb] is increased irt IL12 (tnr)
    *** cells: mSpTh1<xGadd45b> in BMS
    *** does not req: xGadd45b [omission]
    *** source: 12213961-Fig-1c

not Gadd45g--

  *** Stat4[Ab]IP phos(Y693)[phosAb] is increased irt IL12 (tnr)
    *** cells: mSpTh1<xGadd45g> in BMS
    *** does not req: xGadd45g [omission]
    *** source: 12213961-Fig-1c

reqs Ripk2--

  *** Stat4[Ab]IP Yphos[pYAb] is increased irt IL12 (15 min)
    *** cells: mSpTh1 in BMS
    *** partially reqs: Ripk2 [KO]
    *** source: 11894097-Fig-3e

Stat4 features

  *** xStat4[Ab]IP phos(Y693)[phosAb] is increased irt IL12 (45 min)
    *** cells: mSpCs<Stat4~null><xStat4> in BMS
    *** inhibited by: xStat4(Y693F)[phosAb] [substitution]
    *** unaffected by: xStat4(S721A)[phosAb] [substitution]
    *** source: 12213961-Fig-2b
  *** xStat4[Ab]IP Yphos[pYAb] is increased irt IL12 (times)
    *** cells: HEK293T<xStat4> in BMS
    *** times: 0+ 5+ 45++ min
    *** bkg inhibited by: xStat4(Y693F) [substitution]
    *** unaffected by: xStat4(S721A) [substitution]
    *** source: 10961885-Fig-1b

Chemicals

  *** Stat4[Ab]IP phos[WBMS] is increased irt IL12 (times)
    *** cells: hPBTCs in BMS
    *** times: 0 5 15+ 45++ min
    *** unaffected by: PD98059 [pretreatment]
    *** source: 10961885-Fig-2d
  *** Stat4[Ab]IP Yphos[pYAb] is increased irt IL12 (30 min)
    *** cells: 3F6 in BMS
    *** unaffected by: CHX [pretreatment]
    *** inhibited by: Genistein [pretreatment]
    *** source: 10097133-Fig-1b
  *** Stat4[phosAb] phos(Y693)[phosAb] is increased irt IL12 (1 hr)
    *** cells: hPBTCs in BMS "preactivated with Cd3/Cd28" "nuclear-extracts"
    *** unaffected by: CsA [pretreatment]
    *** unaffected by: Rapamycin [pretreatment]
    *** source: 15522880-Fig-4b

Effect of Cotreatments

  *** Stat4[phosAb] phos(Y693)[phosAb] is increased irt IL12 (24 hr)
    *** cells: hCBLs<Cd4~pos> in BMS "containing anti-Cd3/anti-Cd28"
    *** inhibited by: anti-Ifng [cotreatment]
    *** source: 16220539-Fig-2b
  *** Stat4[phosAb] phos(Y693)[phosAb] is increased irt IL12 (48 hr)
    *** cells: hCBLs<Cd4~pos> in BMS "containing anti-Cd3/anti-Cd28"
    *** partially inhibited by: anti-Ifng [cotreatment]
    *** source: 16220539-Fig-2b

No extras

  *** Stat4[Ab]IP phos(Y693)[phosAb] is increased irt IL12 (tnr)
    *** cells: mSpTh1 in BMS
    *** source: 12213961-Fig-1c
  *** Stat4[Ab]IP Yphos[pYAb] is increased irt IL12 (5 min)
    *** cells: hPBTCs in BMLS
    *** source: 10849428-Fig-1d
  *** Stat4[Ab]IP Yphos[pYAb] is increased irt IL12 (15 min)
    *** cells: KIT225 in BMLS "IL2-deprived"
    *** source: 7760829-Fig-4a
  *** Stat4[Ab]IP Yphos[pYAb] is increased irt IL12 (times)
    *** cells: hPHAblasts<Cd3e~neg> in BMS
    *** times: 0 5+ 15+ 30+ min
    *** source: 7638186-Fig-3a
  *** Stat4[Ab]IP Yphos[pYAb] is increased irt IL12 (times)
    *** cells: KIT225CK6 in BMS
    *** times: 0 5+ 30+ min
    *** source: 7638186-Fig-3b
  *** Stat4[Ab]IP Yphos[pYAb] is increased irt IL12 (tnr)
    *** cells: 3F6 in BMS
    *** source: 7722452-Fig-2a
  *** Stat4[Ab]IP Yphos[pYAb] is increased irt IL12 (tnr)
    *** cells: 3F6 in BMS
    *** source: 7722452-Fig-2b

=====================
Total Phosphorylation
=====================

  *** Stat4[Ab] phos[WBMS] is increased irt IL12 (20 min)
    *** cells: hPHAblasts in BMS
    *** comment: response inhibited when IL4 was added to PHA during blasting
    *** source: 9454765-Fig-4b
  *** Stat4[Ab] phos[WBMS] is increased irt IL12 (20 min)
    *** cells: hPHAblasts in BMS
    *** source: 9454765-Fig-4a



