=================
Required Proteins
=================

Traf6--

  *** Tak1[Ab]IP ubiq[UbAb] is increased irt IL1 (times)
    *** cells: mEFs<Traf6~null><xTraf6> in BMS
    *** times: 0 10+ 20++ min
    *** bkg reqs: xTraf6 [omission]
    *** source: 19843958-Fig-1c
  *** Tak1[Ab]IP ubiq[UbAb] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1><xTraf6> in BMLS
    *** times: 0 5++ 10+ min
    *** partially inhibited by: xTraf6(C70A)"E3-mutant" [substitution]
    *** source: 22069318-Fig-2a

Ubc13--

  *** Tak1[Ab]IP ubiq[UbAb] is increased irt IL1 (times)
    *** cells: mEFs in BMS
    *** times: 0 10+ 20++ min
    *** reqs: Ubc13 [KO]
    *** source: 19843958-Fig-4a

Tak1 attributes

  *** xTak1[Ab]IP ubiq[tUbAb] is increased irt IL1 (20 min)
    *** cells: mEFs<Tak1~null><xUbiq(K63only)> in BMS
    *** comment: lysates were boiled to remove noncovalently attached proteins before IP
    *** unaffected by: xTak1(K52R) [substitution]
    *** unaffected by: xTak1(K150R) [substitution]
    *** unaffected by: xTak1(K158R) [substitution]
    *** unaffected by: xTak1(K257R) [substitution]
    *** unaffected by: xTak1(K520R) [substitution]
    *** source: 19843958-Fig-S2
  *** xTak1[Ab]IP ubiq[tUbAb] is increased irt IL1 (20 min)
    *** cells: mEFs<Tak1~null><xUbiq(K63only)> in BMS
    *** comment: lysates were boiled to remove noncovalently attached proteins before IP
    *** unaffected by: xTak1(K172R) [substitution]
    *** inhibited by: xTak1(K209R) [substitution]
    *** source: 19843958-Fig-1e
  *** xTak1[tAb]IP ubiq[UbAb] is increased irt IL1b (times)
    *** cells: HELA<xTak1> in BMS
    *** times: 0 2+ 5++ min
    *** inhibited by: xTak1(K158R) [substitution]
    *** source: 20038579-Fig-4b
  *** xTak1[tAb]IP ubiq[tUbAb] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1><xTak1><xUbiq> in BMLS
    *** times: 0+ 5++ 10++ min
    *** bkg inhibited by: xTak1(K34R) [substitution]
    *** comment: lysates were boiled in 1% SDS to remove noncovalently attached proteins before IP
    *** source: 22069318-Fig-3a

==================
Ubiq is K63-linked
==================

  *** Tak1[Ab]IP ubiq[tUbAb] is increased irt IL1 (times)
    *** cells: mEFs<xUbiq(K63only)> in BMS
    *** comment: lysates were boiled to remove noncovalently attached proteins before IP
    *** times: 0 10+ 20++ min
    *** source: 19843958-Fig-1b
  *** xTak1[tAb]IP ubiq[UbAb] is increased irt IL1b (times)
    *** cells: HEK293T<xTak1><xUbiq(K63only)> in BMS
    *** times: 0 2+ 5++ 10+ 30 min
    *** inhibited by: Ubiq(K48only) [substitution]
    *** source: 20038579-Fig-1d

no extras

  *** xTak1[tAb]IP ubiq[UbAb] is increased irt IL1b (times)
    *** cells: HELA<xTak1> in BMS
    *** times: 0+ 2++ 5++ 10+ 30+ min
    *** source: 20038579-Fig-1b
  *** xTak1[tAb]IP ubiq[tUbAb] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1><xTak1><xUbiq> in BMLS
    *** times: 0 5++ 10+ min
    *** comment: lysates were boiled in 1% SDS to remove noncovalently attached proteins before IP
    *** source: 22069318-Fig-1c


