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Irak1-ubiq
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need evidence that this reqs Traf6
need location information

not Tak1

  *** Irak1[Ab] upshift[WBMS] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 5+ 15 30 min
    *** does not req: Tak1 [KO]
    *** source: 16186825-Fig-3a

not Tax1bp1--

  *** Irak1[Ab]IP ubiq[K63UbAb] is increased irt IL1 (times)
    *** cells: mEFs in BMS
    *** times: 0 5++ 10+ 15 30 60 min
    *** does not req: Tax1bp1 [KO]
    *** source: 20185725-Fig-S2b

not Tnfaip3--

  *** Irak1[Ab]IP ubiq[K63UbAb] is increased irt IL1 (times)
    *** cells: mEFs in BMS
    *** times: 0 5++ 10+ 15 30 60 min
    *** does not req: Tnfaip3 [KO]
    *** source: 20185725-Fig-S2b

Irak1-Ubiq-binding site info

  *** xIrak1[Ab]IP ubiq[UbAb] is increased irt IL1b (5 min)
    *** cells: mEFs<Irak1~null> in BMS
    *** inhibited by: xIrak1(K134R)"Ubiq-binding-site-mut" [substitution]
    *** unaffected by: xIrak1(K180R)"Ubiq-binding-site-mut" [substitution]
    *** inhibited by: xIrak1(K134R/K180R)"Ubiq-binding-site-mut" [substitution]
    *** source: 18347055-Fig-4b

Irak1-Ubiq is K63 linked

  *** Irak1[Ab]IP ubiq[tUbAb] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1><xUbiq> in BMLS
    *** times: 0 5+ 10++ 30+++ min
    *** inhibited by: xUbiq(K63R) [substitution]
    *** unaffected by: xUbiq(K48R) [substitution]
    *** source: 18180283-Fig-3
  *** Irak1[Ab]IP ubiq[tUbAb] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1><xTLR4><xUbiq(K63only)> in BMS
    *** times: 0 2+ 5++ 10++ 20++ min
    *** source: 18347055-Fig-1a

other

  *** Irak1[Ab]IP ubiq[UbAb] is increased irt IL1b (30 min)
    *** cells: HEK293 in BMS
    *** source: 11518704-Fig-1c
  *** Irak1[Ab]IP ubiq[UbAb] is increased irt IL1b (5 min)
    *** cells: HEK293<xIL1R1><xTLR4> in BMS
    *** source: 18347055-Fig-1b
  *** Irak1[Ab]IP ubiq[UbAb] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0 10+ 30++ min
    *** unaffected by: MG132 [addition]
    *** source: 18180283-Fig-4b


==============================================================
Irak1 upshift (phos/ubiq/sumo??) is increased in response to IL1
==============================================================

partiallly requires Irak1 kinase activity

  *** xIrak1[Ab] upshift[WBMS] is increased irt IL1b (times)
    *** cells: HEK293<Irak1~null> in BMS
    *** times: 0 3+ 10+ 30++ 60++ min
    *** partially inhibited by: xIrak1(K239A)"KD" [substitution]
    *** source: 10373513-Fig-10b
  *** xIrak1(K239A)[Ab] upshift[WBMS] is increased irt IL1b (30 min)
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** source: 11518704-Fig-8

requires Irak4 but not Irak4 kinase activity

  *** Irak1[Ab] upshift[WBMS] is increased irt IL1b (times)
    *** cells: hFibros<Irak4~null><xIrak4> in BMS
    *** times: 0 30+ 60+ min
    *** reqs: xIrak4 [omission]
    *** unaffected by: xIrak4(K213A/K214A)"KD" [substitution]
    *** source: 15084582-Fig-5

partially requires Myd88

  *** xIrak1(T66A)[Ab] upshift[WBMS] is detectable
    *** cells: HEK293T<xIL1R1><xIL1Rap><xMyd88> in BMS
    *** partially reqs: xMyd88 [omission]
    *** source: 10854325-Fig-4c

does not require Tak1

  *** Irak1[Ab] upshift[WBMS] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 5+ 15 30 min
    *** repressed by: Tak1 [KO]
    *** source: 16186825-Fig-3a

  *** Irak1[Ab] upshift[WBMS] is increased irt IL1b (times)
    *** cells: HEK293<xTLR3> in BMS
    *** times: 0 30+ 60+ 120+ min
    *** source: 12609980-Fig-1e 
  *** Irak1[Ab]IP upshift[WBMS] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0 2 5+ 10+ 20++ 30++ 40++ 50++ 60++ min
    *** source: 18180283-Fig-1b
  *** Irak1[Ab] upshift[WBMS] is increased irt IL1b (times)
    *** cells: HEK293 in BMS
    *** times: 0 3+ 10+ 30++ 60++ min
    *** source: 10373513-Fig-10a
  *** Irak1[Ab]IP upshift[WBMS] is increased irt IL1b (5 min)
    *** cells: EL4 in BMS
    *** source: 15896328-Fig-1a
  *** xIrak1[Ab]IP upshift[WBMS] is increased irt IL1b (5 min)
    *** cells: EL4 in BMS
    *** source: 15896328-Fig-1a
  *** Irak1[Ab] upshift[WBMS] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 5 10+ 20+ 30+ 40+ min
    *** source: 14625308-Fig-6a
  *** Irak1[Ab] upshift[WBMS] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 5 10++ 30+ 60+ 120+ 240 min
    *** source: 14625308-Fig-6b
  *** Irak1[Ab] upshift[WBMS] is increased irt IL1b (times)
    *** cells: HEK293 in BMS
    *** times: 0 1 2+ 5++ 10++ 30+++ 60++ 120++ 240++ min
    *** source: 12242293-Fig-2a
  *** Irak1[Ab]IP upshift[WBMS] is increased irt IL1b (30 min)
    *** cells: HEK293 in BMS
    *** source: 11518704-Fig-1c
  *** Irak1[Ab]IP upshift[WBMS] is increased irt IL1b (times)
    *** cells: HEK293 in BMS
    *** times: 0 10+ 30+ 60+ 120+ 240+ min
    *** source: 11518704-Fig-5a
  *** Irak1[Ab] upshift[WBMS] is increased irt IL1b (15 min)
    *** cells: HEK293 in BMS
    *** source: 11518704-Fig-1b
  *** Irak1[Ab] upshift[WBMS] is increased irt IL1b (30 min)
    *** cells: HEK293 in BMS
    *** source: 11518704-Fig-6b
  *** Irak1[Ab] upshift[WBMS] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 1 2+ 5++ 10++ 20+++ min
    *** source: 11259596-Fig-5a
  *** Irak1[Ab]IP upshift[WBMS] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 1+ 2+ 5++ 10++ 20++ min
    *** source: 11259596-Fig-5b
  *** Irak1[Ab] upshift[WBMS] is increased irt IL1b (times)
    *** cells: HEK293<xTLR8> in BMS
    *** times: 0 5+ 15+ 30+ 60+ 120+ min
    *** source: 16737960-Fig-2e
  *** Irak1[Ab]IP upshift[WBMS] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0 2 5 10+ 20++ 30++ 40++ 50++ 60++ min
    *** source: 18180283-Fig-1b
  *** Irak1[Ab] upshift[WBMS] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0 10+ 30++ min
    *** unaffected by: MG132 [addition]
    *** source: 18180283-Fig-4a
  ---------------------------------------------------------------
  *** Irak1[Ab]IP upshift[WBMS] is increased itpo (xIL1R1 + xIL1Rap)
    *** cells: HEK293 in BMS
    *** reqs: both [omission]
    *** inhibited by: xIL1Rap(1-403) [substitution]
    *** source: 9371760-Fig-1c

xIrak1 characteristics required for upshift

  *** xIrak1(del(104-198))"dUD"[Ab] upshift[WBMS] is unchanged irt IL1b (15 min)
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** source: 11518704-Fig-1b
  *** xIrak1(K239A)"KD"[Ab] upshift[WBMS] is increased irt IL1 (times)
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** times: 0 5 15+ 30++ 60++ min
    *** comment: total Irak prot-exp decreases with time
    *** source: 17197697-Fig-2e
  *** xIrak1(K239A)"KD"[Ab] upshift[WBMS] is increased irt IL1 (times)
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** times: 0 10+ 30+ min
    *** inhibited by: xIrak1(K32R/K134R/K179R)"Ubiq-mut" [substitution]
    *** source: 17197697-Fig-2e
  *** xIrak1(K239A/S131A/S137A/S138A/S140A/T141A/S144A)[Ab] upshift[WBMS] is increased irt IL1 (times)
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** times: 0+ 5++ 15++ 30++ 60++ min
    *** comment: total Irak prot-exp does not decreased with time
    *** source: 17197697-Fig-2e






