=========================================================
IKK kinase activity reqs Ikk1--  ?    does substrate matter?
=========================================================

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (20 min)
    *** cells: mEFs in BMS
    *** does not req: Ikk1 [KO]
    *** source: 11733537-Fig-4b
  *** Nemo[Ab]IP IVKA(Rela)[32P-ATP] is increased irt IL1 (20 min)
    *** cells: mEFs in BMS
    *** reqs: Ikk1 [KO]
    *** source: 11733537-Fig-4b

=============================
IKK kinase activity reqs Ikk2--
=============================

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (20 min)
    *** cells: mEFs in BMS
    *** partially reqs: Ikk2 [KO]
    *** source: 11733537-Fig-4b
  *** Nemo[Ab]IP IVKA(Rela)[32P-ATP] is increased irt IL1 (20 min)
    *** cells: mEFs in BMS
    *** reqs: Ikk2 [KO]
    *** source: 11733537-Fig-4b

======================================
IKK kinase activity and phos reqs Nemo--
======================================

  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (times)
    *** cells: mESCs in BMS
    *** times: 0 10+ 30++ 60+ min
    *** reqs: Nemo [KO]
    *** source: 10805784-Fig-4a
  *** Ikks[phosAb] phos(sitenr)[phosAb] is increased irt IL1b (times)
    *** cells: mEFs<Nemo~null><xNemo> in BMS
    *** times: 0 5++ 20+ min
    *** reqs: xNemo [omission]
    *** unaffected by: xNemo(K285R) [substitution]
    *** source: 19112497-Fig-6a

=======================================
IKK kinase activity and phos reqs Irak1--  ????
=======================================

  *** Ikks[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (times)
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** times: 0 7++ 15+ min
    *** source: 17197697-Fig-6c
  *** Ikks[phosAb] phos(SLCTS)[phosAb] is unchanged irt IL1 (times)
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** times: 0 10++ 30++ 120+ min
    *** source: 17197697-Fig-6a

======================================================
IKK kinase-activity and phos reqs Tak1-- kinase-activity
======================================================

  prot-exp

  *** Ikk2[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 15+ 30+ min
    *** reqs: Tak1 [KO]
    *** source: 16260493-Fig-5a
  *** Ikks[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (tnr)
    *** cells: mEFs in BMS
    *** partially reqs: Tak1 [KO]
    *** source: 17197697-Fig-8d
  *** Ikks[phosAb] phos(SLCTS)[phosAb] is increased irt IL1 (times)
    *** cells: mEFs in BMS
    *** times: 0 10++ 30+ 120+ min
    *** partially reqs: Tak1 [KO]
    *** source: 17197697-Fig-6b

  kinase activity

  *** Ikks[phosAb] phos(sitenr)[phosAb] is increased irt IL1b (times)
    *** cells: mEFs in BMLS
    *** times: 0 2 5+ 10++ 30+ min
    *** inhibited by: xTak1(mnr)"KD" [addition]
    *** source: 18180283-Fig-6a

  ubiq sites

  *** Ikks[phosAb] phos(sitenr)[phosAb] is increased irt IL1b (times)
    *** cells: mEFs<Tak1~null><xTak1> in BMS
    *** times: 0 5+ 10++ 15++ 30++ min
    *** reqs: xTak1 [omission]
    *** partially inhibited by: xTak1(K158R) [addition]
    *** source: 20038579-Fig-7b

  phos sites

  *** Ikks[phosAb] phos(sitenr)[phosAb] is increased irt IL1b (times)
    *** cells: mEFs<Tak1~null><xTak1> in BMS
    *** times: 0 5+ 10++ 15++ 30+ min
    *** partially reqs: xTak1 [omission]
    *** partially inhibited by: xTak1(T178A/T184A) [substitution]
    *** source: 18617512-Fig-4d
  --------------------------------------------------------------------

=================================================
IKK kinase-activity reqs Traf6-- E3-ligase-activity
=================================================

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1b (times)
    *** cells: mEFs<Traf6~null><xTraf6> in BMLS
    *** times: 0 2+ 5+ 10+ min
    *** inhibited by: xTraf6(C70A)"E3-inactive RING mutant" [substitution]
    *** inhibited by: xTraf6(K124R) [substitution]
    *** source: 17135271-Fig-7b
  *** Ikks[phosAb] phos(sitenr)[phosAb] is increased irt IL1b (times)
    *** cells: mEFs<Traf6~null><xTraf6> in BMS
    *** times: 0 5+ 10+ min
    *** reqs: xTraf6 [omission]
    *** inhibited by: xTraf6(C70A) [substitution]
    *** unaffected by: xTraf6(mnr)"K32-518R" [substitution]
    *** source: 19112497-Fig-3d
  *** Ikks[phosAb] phos(SLCTS)[phosAb] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1><xTraf6> in BMLS
    *** times: 0 5+ 10++ min
    *** inhibited by: xTraf6(C70A)"E3-mutant" [substitution]
    *** source: 22069318-Fig-2b

not Ube2ds--

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1b (times)
    *** cells: U2OS in BMS
    *** times: 0 5+ 15++ min
    *** does not req: Ube2ds [RNAi]
    *** source: 19854138-Fig-4a

Ube1--

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1b (times)
    *** cells: U2OS in BMS
    *** times: 0 5+ 10+ min
    *** reqs: Ube1 [RNAi]
    *** source: 19854138-Fig-S3c

Ubc13--

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1b (times)
    *** cells: U2OS in BMS
    *** times: 0 10+ 20++ min
    *** reqs: Ubc13 [RNAi]
    *** source: 19854138-Fig-3a

K63Ubiq--

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1b (times)
    *** cells: U2OS in BMS
    *** times: 0 5+ 15+ min
    *** reqs: Ubiq [RNAi]
    *** source: 19854138-Fig-2a,2b
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1b (times)
    *** cells: U2OS<Ubiq~null><xUbiq> in BMS
    *** times: 0 5+ 15+ min
    *** inhibited by: xUbiq(K63R) [substitution]
    *** source: 19854138-Fig-2b


============================
Things that are not required
============================

IKK kinase-activity does not req Mekk1

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (times)
    *** cells: mESCs in BMS
    *** times: 0 10+ 30++ 60++ min
    *** unaffected by: kMekk1(1-1188)"del-kinase-domain" [addition]
    *** source: 10805784-Fig-3a

IKK kinase-activity does not req Mekk3

  *** Ikks[phosAb] phos(S176/S180)[phosAb] is increased irt IL1b (times)
    *** cells: mEFs<xTLR8> in BMS
    *** times: 0 15++ 30+ min
    *** does not req: Mekk3 [KO]
    *** source: 16737960-Fig-4b

IKK kinase-activity does not req Nik

  *** Ikks[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (5 min)
    *** cells: mEFs in BMS
    *** does not req: Nik [KO]
    *** source: 11251123-Fig-1c

IKK kinase-activity does not req Pi3k?  does substrate matter?

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (20 min)
    *** cells: mEFs in BMS
    *** unaffected by: LY294002 [addition]
    *** source: 11733537-Fig-4b
  *** Nemo[Ab]IP IVKA(Rela)[32P-ATP] is increased irt IL1 (20 min)
    *** cells: mEFs in BMS
    *** inhibited by: LY294002 [addition]
    *** source: 11733537-Fig-4b

IKK kinase-activity does not req Pkcz

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (30 min)
    *** cells: mEFs in BMS
    *** does not req: Pkcz [KO]
    *** source: 11684013-Fig-3a

IKK kinase-activity does not req Ripk1--

  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (5 min)
    *** cells: mEFs in BMS
    *** does not req: Ripk1 [KO]
    *** source: 10795740-Fig-1d

IKK kinase-activity does not req Tab1

  *** Ikk2[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 15+ 30+ min
    *** does not req: Tab1 [KO]
    *** source: 16260493-Fig-5a

IKK kinase-activity does not req Tab2

  *** Ikk2[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 15+ 30+ min
    *** does not req: Tab2 [KO]
    *** source: 16260493-Fig-5a

IKK kinase-activity does not req Traf2 or Traf5

  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (times)
    *** cells: mEFs in BMS
    *** times: 0 10+ min
    *** does not req: Traf2 or Traf5 [DKO]
    *** source: 11479302-Fig-1b
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (5 min)
    *** cells: mEFs in BMS
    *** does not req: Traf2 [KO]
    *** source: 10795740-Fig-1d

===============
Ikk1 phos-sites
===============

  *** xIkk1[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (10 min)
    *** cells: HELA in BMS
    *** unaffected by: xIkk1(S176A/S180A) [substitution]
    *** unaffected by: xIkk1(S176A) [substitution]
    *** unaffected by: xIkk1(S180A) [substitution]
    *** source: 10195894-Fig-2b
  *** Ikks[phosAb] phos(SLCTS)[phosAb] is increased irt IL1 (15 min)
    *** cells: mEFs in BMS
    *** comment: phos appears in two bands, top band reqs Ikk2 [KO]
    *** comment: top phos band reappears in KO cells itpo xIkk2
    *** comment: top phos band does not reappear in KO cells itpo xIkk2(mnr)"KD"
    *** source: 17197697-Fig-7b

==================================
other IKK kinase-activity and phos
==================================

  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 1 2+ 5++ 15++ 20+ min
    *** source: 11259596-Fig-8b
  *** Ikk1[Ab]IP IVKA(Rela)[32P-ATP] is increased irt IL1a (10 min)
    *** cells: HELA in BMS
    *** source: 15489227(D)
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (10 min)
    *** cells: HELA in BMS
    *** source: 10195894-Fig-2b
  *** Nemo[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1b (times)
    *** cells: HELA in BMS
    *** times: 0 2+ 5++ 10++ 20 min
    *** source: 15327770(D)
  *** Ikks[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1b (times)
    *** cells: HEK293<xTLR8> in BMS
    *** times: 0 7++ 15+ min
    *** source: 16737960-Fig-4e
  *** Ikks[phosAb] phos(SLCTS)[phosAb] is increased irt IL1b (times)
    *** cells: HEK293<xTLR8> in BMS
    *** times: 0 15++ 30+ min
    *** source: 16737960-Fig-4a
  *** Ikks[phosAb] phos(sitenr)[phosAb] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0 5+ 10++ min
    *** source: 18180283-Fig-1c
  *** Ikk2[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 5+ 15+ min
    *** source: 14661019-Fig-3e
  *** Ikks[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (times)
    *** cells: HEK293 in BMS
    *** times: 0 7++ 15+ min
    *** source: 17197697-Fig-6c
  *** Ikks[phosAb] phos(SLCTS)[phosAb] is increased irt IL1 (times)
    *** cells: HEK293 in BMS
    *** times: 0 10++ 30++ 120+ min
    *** source: 17197697-Fig-6a
  *** Ikks[phosAb] phos(CTSFV)[phosAb] is increased irt IL1 (times)
    *** cells: mEFs in BMS
    *** times: 0 5++ 10++ 20+ 30 min
    *** source: 19843958-Fig-3d
  *** Ikk1[Ab]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (5 min)
    *** cells: mEFs in BMS
    *** source: 10795740-Fig-1d
  *** Ikks[phosAb] phos(SLCTS)[phosAb] is increased irt IL1 (5 min)
    *** cells: hSNFs in BMS
    *** inhibited by: 5Z7Oxozeaenol [addition]
    *** source: 17197697-Fig-6e
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (10 min)
    *** cells: HELA<xIkk1> in BMS
    *** source: 9346241-Fig-2b
  *** xIkk1[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (times)
    *** cells: HELA<xIkk1> in BMS
    *** times: 0 1 5++ 15++ 30++ 60+ 120+ min
    *** source: 9346241-Fig-2c
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt IL1 (times)
    *** cells: HELA<xIkk1> in BMS
    *** times: 0 1 5++ 15++ 30++ 60+ 120+ min
    *** source: 9346241-Fig-2c
  *** xIkk1[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: HEK293<xIkk1> in BMS
    *** source: 9346241-Fig-3b
  *** xIkk1[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: HEK293<xIkk1> in BMS
    *** partially inhibited by: xIkk1(K44M) [substitution]
    *** source: 9346241-Fig-5b
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: HELA<xIkk1> in BMS
    *** source: 9346241-Fig-2b
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: HEK293<xIkk2> in BMS
    *** source: 9346241-Fig-3b
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: HELA<xIkk2> in BMS
    *** source: 9346241-Fig-3c
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: HEK293<xIkk2> in BMS
    *** partially inhibited by: xIkk1(K44M) [substitution]
    *** source: 9346241-Fig-5b
  *** xIkk1[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: HELA<xIkk1> in BMS
    *** times: 0 1 5++ 15++ 30+ 60+ 120+ min
    *** source: 9346241-Fig-2c
  *** xIkk2[tAb]IP IVKA(Ikba)[32P-ATP] is increased irt Tnf (times)
    *** cells: HELA<xIkk1> in BMS
    *** times: 0 1 5++ 15++ 30+ 60+ 120+ min
    *** source: 9346241-Fig-2c

