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Tyrosine phosphorylation in response to Egf
===========================================

Yphos

  *** Shp2[Ab] Yphos[pYAb-ppt] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** partially inhibited by: Wortmannin [addition] no-bkg-control
    *** partially inhibited by: LY294002 [addition] no-bkg-control
    *** source: 11134009-Fig-5b
  *** xShp2[Ab]IP Yphos[pYAb] is increased irt Egf (10 min)
    *** cells: COS1 in BMLS
    *** enhanced by: xShp2(R465E) [substitution]
    *** enhanced by: xShp2(C459S) [substitution]
    *** enhanced by: xShp2(C459S/R465E) [substitution]
    *** source: 14560030-Fig-4a
  *** Shp2[Ab]IP Yphos[pYAb] is increased irt Egf (10 min)
    *** cells: COS1 in BMLS
    *** source: 14560030-Fig-4a

phos(Y546)

  *** Shp2[phosAb] phos(Y546)[phosAb] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 1+ 5+ 10+ min
    *** partially inhibited by: Wortmannin [addition]
    *** source: 16687399-Fig-4a

==============================================================================
Evidence for recruitment to the EgfR complex: 
increased coppt to other members of the EgfR complex only
==============================================================================

Gab1 coppt   14665621 makes a case for Gab1(Y627/Y659) req for Shp2 to EgfRC

  *** Shp2[Ab] copptby[WB] Gab1[Ab] is increased irt Egf (4 min)
    *** cells: COS7 in BMLS
    *** source: 10788507(D)
  *** Shp2[Ab] copptby[WB] Gab1[Ab] is increased irt Egf (10 min)
    *** cells: MIMCD3 in BMLS
    *** inhibited by: U0126 [addition]
    *** source: 11896055(D)
  *** Shp2[Ab] copptby[WB] Gab1[Ab] is increased irt Egf (10 min)
    *** cells: T47D in BMLS
    *** source: 15952937(D)
  *** Shp2[Ab] copptby[WB] Gab1[Ab] is increased irt Egf (10 min)
    *** cells: PC12 in BMLS
    *** source: 11353842-Fig-2d
  *** Shp2[Ab] copptby[WB] Gab1[Ab] is increased irt Egf (5 min)
    *** cells: U87MG in BMLS
    *** source: 14701753-Fig-1a
  *** Shp2[Ab] copptby[WB] Gab1[Ab] is increased irt Egf (times)
    *** cells: MDAMB468 in BMLS
    *** times: 0 5+ 10+ 30+ min
    *** source: 14665621-Fig-2a
  *** Shp2[Ab] copptby[WB] Gab1[Ab] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** partially inhibited by: Wortmannin [addition] no-bkg-control
    *** partially inhibited by: LY294002 [addition] no-bkg-control
    *** source: 11134009-Fig-6b
  *** Shp2[Ab] copptby[WB] xGab1[tAb] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: xGab1(104-694)"PH-domain-mutant" [substitution]
    *** inhibited by: Wortmannin [addition] no-bkg-control
    *** source: 11134009-Fig-7b
  --- reqs Gab1(Y627/Y659)
  *** Shp2[Ab] copptby[WB] xGab1[tAb] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: xGab1(Y627F) [substitution]
    *** source: 11134009-Fig-6e
  *** Shp2[Ab] copptby[WB] xGab1[tAb] is increased irt Egf (10 min)
    *** cells: mEFs<xGab1> in BMLS
    *** inhibited by: xGab1(Y627F/Y659F) [substitution]
    *** unaffected by: xGab1(Y447F/Y472F/Y589F) [substitution]
    *** source: 12629518(D)
  *** Shp2[Ab] copptby[WB] xGab1[tAb] is increased irt Egf (times)
    *** cells: COS7 in BMLS
    *** times: 0 1++ 3++ 30+ min
    *** inhibited by: xGab1(Y627F) [substitution]
    *** inhibited by: xGab1(Y659F) [substitution]
    *** source: 11323411-Fig-7b
  *** Shp2[Ab] copptby[WB] xGab1[tAb] is increased irt Egf (3 min)
    *** cells: COS7 in BMLS
    *** inhibited by: xGab1(Y627F)"Shp2-BS-mut" [substitution]
    *** source: 10788507(D)

Gab2 coppt

  *** Shp2[Ab] copptby[WB] Gab2[Ab] is increased irt Egf (10 min)
    *** cells: T47D in BMLS
    *** source: 15952937(D)
  *** Shp2[Ab] copptby[WB] xGab2[tAb] is increased irt Egf (1 min)
    *** cells: rHepatocytes in BMLS
    *** inhibited by: xGab2(Y603F/Y632F) [substitution]
    *** source: 12464621-Fig-7a


Grb2 coppt

  *** Shp2[MS] snaggedby[WB] rGrb2(mnr)"SH2"[GST] is increased irt Egf (10 min)
    *** cells: HELA in BMLS
    *** source: 12577067(D)
  *** Shp2[Ab] copptby[WB] Grb2[Ab] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** partially inhibited by: Wortmannin [addition] no-bkg-control
    *** partially inhibited by: LY294002 [addition] no-bkg-control
    *** source: 11134009-Fig-5a

Pxn coppt

  *** Shp2[Ab] copptby[WB] Pxn[Ab] is increased irt Egf (10 min)
    *** cells: MDAMB468 in BMLS
    *** inhibited by: xGab1(Y627F/Y659F) [addition]
    *** source: 14665621-Fig-7c
  *** Shp2[Ab] copptby[WB] Pxn[Ab] is increased irt Egf (10 min)
    *** cells: MDAMB468 in BMLS
    *** source: 14665621-Fig-4b
  *** Shp2[Ab] copptby[WB] Pxn[Ab] is increased irt Egf (10 min)
    *** cells: MDAMB468 in BMLS
    *** source: 14665621-Fig-7a
  *** Shp2[Ab] copptby[WB] Pxn[Ab] is increased irt Egf (times)
    *** cells: MDAMB468 in BMLS
    *** times: 0 5+ 10+ 30+ min
    *** source: 14665621-Fig-2a

Stat1 coppt

  *** Shp2[Ab] copptby[WB] Stat1[Ab] is increased irt Egf (tnr)
    *** cells: A431 in BMS
    *** source: 12270932-Fig-1d
  *** Shp2[Ab] copptby[WB] xStat1[Ab] is increased irt Egf (tnr)
    *** cells: HEK293 in BMS
    *** source: 12270932-Fig-3d

===========
later times
===========

  *** Shp2[Ab] infraction(nuclear)[WB] is increased irt Egf (times)
    *** cells: A431 in BMS
    *** times: 0+ 30++ 60++ 180++ 240++ min
    *** source: 12270932-Fig-2b
  *** Shp2[Ab] locatedin(nucleus)[IHC] is increased irt Egf (30 min)
    *** cells: A431 in BMS
    *** source: 12270932-Fig-2a
