===========================================================
requirement for a HrasGef is obtained from common-evidence
===========================================================
assumption that Gab2 can replace Gab1 is based on similarity
with rules 639 and 196
===========================================================
===========================================================

not Abi1--

  *** xHras[tAb] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** partially inhibited by: xAbi1 [addition]
    *** source: 11777939-Fig-4b

Efna1--   Efna1Fc inhibition

  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: RD in BMLS
    *** inhibited by: Efna1Fc [pretreatment]
    *** source: 23103856-Fig-S3a
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: HBL100 in BMLS
    *** inhibited by: Efna1Fc [pretreatment]
    *** source: 23103856-Fig-S3a

Gab1 phos(Y627)--

  *** xHras[tAb] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: VERO<xGab1> in BMLS
    *** inhibited by: xGab1(Y627F) [substitution] no-bkg-control
    *** source: 15574420-Fig-5a
  *** xHras[tAb] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: VERO<xGab1> in BMLS
    *** inhibited by: xGab1(Y627F) [substitution] no-bkg-control
    *** source: 15574420-Fig-3c
  *** xRas?[tAb] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: VERO<xGab1><xRas?> in BMLS
    *** inhibited by: xGab1(Y627F) [substitution]
    *** inhibited by: xGab1(Y627F/Y447F/Y472F/Y619F) [substitution]
    *** partially inhibited by: xGab1(Y447F/Y472F/Y619F) [substitution]
    *** source: 15574420-Fig-1a
  *** xHras[tAb] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: VERO<xGab1> in BMLS
    *** unaffected by: xGab1 [addition]
    *** inhibited by: xGab1(Y627F) [addition]
    *** inhibited by: xGab1(Y447F/Y472F/Y589F) [addition]
    *** source: 11134009-Fig-6c
  *** xRas?[tAb] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: VERO<xGab1><xRas?> in BMLS
    *** inhibited by: xGab1(Y627F) [substitution]
    *** inhibited by: xGab1(Y627F/Y447F/Y472F/Y619F) [substitution]
    *** partially inhibited by: xGab1(Y447F/Y472F/Y619F) [substitution]
    *** source: 15574420-Fig-1a
  *** xRas?[tAb] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: VERO<xGab1><xRas?> in BMLS
    *** inhibited by: xGab1(Y627F) [substitution]
    *** unaffected by: xGab1(del(161-318)) [substitution]
    *** unaffected by: xGab1(del(161-318)/Y627F) [substitution]
    *** source: 15574420-Fig-5a
  *** xRas?[tAb] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: VERO<xRas?><xGab1> in BMLS
    *** unaffected by: xRasa1(R789Q/R903Q) [addition]
    *** inhibited by: xGab1(Y627F) [substitution]
    *** source: 15574420-Fig-3c
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 1++ 2++ 5+ min
    *** partially reqs: Gab1 [KO]
    *** source: 12629518(D)

Gna12--    Gna12(Q22L) ???

  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: COS7 in BMS
    *** partially inhibited by: xGna12(Q22L) [addition]
    *** source: 9796816-Fig-6e
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: MEG01 in BMS
    *** unaffected by: xGna12(Q22L) [addition]
    *** source: 9796816-Fig-7e

not xHrasls2--

  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: HTTA in BMLS
    *** inhibited by: xHrasls2 [addition]
    *** source: 16005186-Fig-3

not xHrasls3--

  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: HTTA in BMLS
    *** inhibited by: xHrasls3 [addition]
    *** source: 16005186-Fig-3

Kras--  ???

  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: HBL100 in BMLS
    *** unaffected by: Kras(G12V) [addition]
    *** source: 23103856-Fig-4b
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: MDAMB231 in BMLS
    *** repressed by: Kras(G13D) [RNAi]
    *** source: 23103856-Fig-S1b
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: MIAPACA2 in BMLS
    *** repressed by: Kras(G12C) [RNAi]
    *** source: 23103856-Fig-S1a

Nras--  ???

  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: HBL100 in BMLS
    *** unaffected by: Nras(G12V) [addition]
    *** source: 23103856-Fig-4b

not Phb--

  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (10 min)
    *** cells: HELA in BMLS
    *** does not req: Phb [RNAi]
    *** source: 16041367-Fig-4g

Pi3k--

  *** xHras[tAb] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: xPik3r?(mnr)"DN" [addition] no-bkg-control
    *** partially inhibited by: xPik3cb(K805R)"KD" [addition] no-bkg-control
    *** source: 11134009-Fig-4b

not Pak1--

  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: NIH3T3 in BMLS
    *** unaffected by: xPak1(83-149)"DN" [addition]
    *** source: 16129686-Fig-3b

not xRarres1--

  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: HTTA in BMLS
    *** inhibited by: xRarres1 [addition]
    *** source: 16005186-Fig-3

not Rasa1--

  *** xHras[tAb] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: xRasa1 [addition]
    *** inhibited by: xRasa1(R903Q) [addition]
    *** unaffected by: xRasa1(R789Q/R903Q) [addition]
    *** source: 15574420-Fig-3b
  *** xHras[tAb] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: VERO<xGab1> in BMLS
    *** unaffected by: xRasa1(R789Q/R903Q) [addition]
    *** source: 15574420-Fig-3c
  *** xHras[tAb] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: VERO<xGab1> in BMLS
    *** unaffected by: xRasa1(R789Q/R903Q) [addition]
    *** source: 15574420-Fig-5a
  *** xRas?[tAb] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: VERO<xRas?> in BMLS
    *** inhibited by: xRasa1 [addition]
    *** unaffected by: xRasa1(R789Q/R903Q) [addition]
    *** inhibited by: xRasa1(R903Q) [addition]
    *** source: 15574420-Fig-3b

not Rig1--

  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: HTTA in BMLS
    *** inhibited by: xRig1 [addition]
    *** source: 16005186-Fig-3
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: HTTA in BMLS
    *** inhibited by: xRig1 [addition]
    *** source: 16005186-Fig-1a
  *** xHras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: HTTA in BMLS
    *** inhibited by: xRig1 [addition]
    *** source: 16005186-Fig-2b

not Rin1--  ???

  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: NR6<xEgfR> in BMLS
    *** unaffected by: xRin1(67-154)"SH2" [addition]
    *** source: 12783862-Fig-6b

Shp2-- PTPase activity

  *** xHras[tAb] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: xShp2(mnr)"CIA" [addition]
    *** enhanced by: xShp2 [addition]
    *** source: 11134009-Fig-5c
  *** xHras[tAb] GTP-association[BDPD] is increased irt Egf (10 min)
    *** cells: COS1 in BMLS
    *** inhibited by: xShp2(C459S)"CIA" [addition]
    *** source: 14560030-Fig-3a
  *** xHras[tAb] GTP-association[BDPD] is increased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0 2+ 10+ 60 240 min
    *** inhibited by: xShp2(C459S)"CIA" [addition]
    *** comment: decrease after 10 min is reversed itpo xShp2 [addition]
    *** source: 14560030-Fig-3b

not Sos1--

  *** Hras[Ab]IP GTP-association[TLC] is increased irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** does not req: Sos1 [KO]
    *** source: 10675333-Fig-6c

inhibited by Spred1--

  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (times)
    *** cells: HEK293 in BMS
    *** times: 0 2++ 5++ 10+ min
    *** inhibited by: xSpred1 [addition]
    *** unaffected by: xSpred1(1-266) [addition]
    *** inhibited by: xSpred1(1-266/CAAX) [addition]
    *** unaffected by: xSpred1(1-266/del(81-85)/CAAX) [addition]
    *** unaffected by: xSpred1(1-266/T102R/CAAX) [addition]
    *** source: 22751498-Fig-2c

chemicals

  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (times)
    *** cells: COS in BMS
    *** times: 0 2++ 5++ 10++ 20+ 60+ min
    *** partially inhibited by: Wortmannin [addition]
    *** partially inhibited by: LY294002 [addition]
    *** source: 11259591-Fig-4a
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 1+++ 3++ 5++ 10+ 30 min
    *** partially inhibited by: Wortmannin [addition]
    *** source: 16687399-Fig-2e
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (times)
    *** cells: COS7 in BMLS
    *** times: 0 5+ 10+ min
    *** unaffected by: PMA (30 min) [stim]
    *** source: 11283245(D)
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: COS1 in BMLS
    *** unaffected by: Monodansylcadaverine [addition]
    *** source: 12574122(D)
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: Wortmannin [addition] no-bkg-control
    *** inhibited by: LY294002 [addition] no-bkg-control
    *** source: 11134009-Fig-4a
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (2 min)
    *** cells: RAT2 in BMLS
    *** unaffected by: U73122 [addition]
    *** source: 9663394-Fig-2b
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** partially inhibited by: LY294002 [pretreatment]
    *** partially inhibited by: Wortmannin [pretreatment]
    *** source: 10330169-Fig-1a
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: MIAPACA2 in BMLS
    *** inhibited by: Erlotinib [pretreatment]
    *** comment: the background and treated are both enhanced itao Kras(G12C) [RNAi]
    *** source: 23103856-Fig-5
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: RD in BMLS
    *** inhibited by: Erlotinib [pretreatment]
    *** comment: the background and treated are both enhanced itao Nras(Q61H) [RNAi]
    *** source: 23103856-Fig-5

=====
other
=====

  *** xHras[tAb] GTP-association[BDPD] is increased itpo xEgfR(Y992F)"CA"
    *** cells: NIH3T3<xShp2(R465E)"DN"> in BMLS
    *** inhibited by: xEgfR(WT) [substitution]
    *** source: 14560030-Fig-8a
  *** xHras[tAb] GTP-association[BDPD] is increased irt Egf (times)
    *** cells: NIH3T3<xEgfR><xShp2(R465E)"DN"> in BMLS
    *** times: 0 2+ 10+ 60+ 240+ min
    *** source: 14560030-Fig-8a

===================================
Is Hras recruited to EgfR irt Egf?
===================================

may be due to xRin1 only

  *** xHras[Ab] copptby[WB] xEgfR[Ab] is increased irt Egf (5 min)
    *** cells: NR6<xEgfR><xRin1><xRab5a><xHras> in BMLS
    *** inhibited by: xRin1(293-783)"C1" [substitution]
    *** source: 12783862-Fig-7a

T24 contains Hras(G12V)

  *** Hras[Ab] GTP-association[BDPD] is detectable-but unchanged irt Egf (5 min)
    *** cells: T24 in BMLS
    *** source: 23103856-Fig-1a
  *** Hras[Ab] GTP-association[BDPD] is detectable-but unchanged irt Egf (5 min)
    *** cells: T24 in BMLS
    *** unaffected by: Efna1Fc [pretreatment]
    *** source: 23103856-Fig-S3a
  *** Hras[Ab] GTP-association[BDPD] is detectable-but unchanged irt Egf (5 min)
    *** cells: T24 in BMLS
    *** unaffected by: Erlotinib [pretreatment]
    *** source: 23103856-Fig-5

no extras

  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (times)
    *** cells: PC12 in BMLS
    *** times: 0 1++ 2+++ 5+ 10+ 30+ 60+ min
    *** source: 11466412-Fig-1b
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (times)
    *** cells: mAstrocytes in BMLS
    *** times: 0 5++ 15++ 40+ 90+ min
    *** source: 16980617-Fig-6b
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (2 min)
    *** cells: A7R5 in BMLS
    *** source: 11907028(D)
  *** xHras[tAb] GTP-association[BDPD] is increased irt Egf (times)
    *** cells: NIH3T3 in BMLS
    *** times: 0 1+ 2+ 5+ min
    *** source: 15226433(D)
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: HELA in BMS
    *** source: 16858395(D)
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (times)
    *** cells: PC12 in BMLS
    *** times: 0 2+ 5+ 7 20 50 min
    *** source: 9774335-Fig-5
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (10 min)
    *** cells: COS7 in BMS
    *** source: 16364920-Fig-2a
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (1 min)
    *** cells: NIH3T3<xEgfR><xAbi1> in BMLS
    *** source: 16182283-Fig-2a
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (1 min)
    *** cells: NIH3T3<xEgfR> in BMLS
    *** source: 16182283-Fig-2a
  *** Hras[Ab]IP GTP-association[TLC] is increased irt Egf (5 min)
    *** cells: PC12<xPdgfRb> in BMLS
    *** unaffected by: Forskolin [addition]
    *** source: 7935374-Fig-7a,7b
  *** Hras[Ab]IP GTP-association[TLC] is increased irt Egf (times)
    *** cells: RAT1 in BMLS
    *** times: 0 2+ 5++ 7++ 15++ 30++ min
    *** source: 7798267-Fig-1c
  *** Hras[Ab]IP GTP-association[TLC] is increased irt Egf (times)
    *** cells: CHO<xInsR><xEgfR> in BMLS
    *** times: 0 1++ 3+ 5 30 min
    *** source: 8663461-Fig-2
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (10 min)
    *** cells: HEK293 in BMS
    *** source: 11889038-Fig-2b
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (times)
    *** cells: HEK293T in BMLS
    *** times: 0 5+ 10+ 20+ 40+ 60 min
    *** source: 11784866-Fig-1c
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (times)
    *** cells: CHO<xEgfR><xInsR> in BMS
    *** times: 0 2++ 5++ 15+ min
    *** source: 15192046-Fig-5a
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (times)
    *** cells: PC12 in BMLS
    *** times: 0 2+++ 5++ 15+ 30+ 60 min
    *** comment: the pattern is the same using the Braf1-RBD or Raf1-RBD
    *** source: 11278445-Fig-3a
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: RD in BMLS
    *** source: 23103856-Fig-1a
  *** Hras[Ab] GTP-association[BDPD] is increased irt Egf (5 min)
    *** cells: MIAPACA2 in BMLS
    *** source: 23103856-Fig-1a
