======================================================
Gab1 is phosphorylated on Tyrosine in response to Egf
======================================================

not Irs1--

  *** xGab1[Ab] Yphos[pYAb-ppt] is increased irt Egf (5 min)
    *** cells: mEFs<Irs1~null> in BMLS
    *** source: 10744748-Fig-2a

Gnai1 and/or Gnai3--

  *** Gab1[Ab]IP phos(Y627)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+ 5+ min
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** source: 19401591-Fig-5b
  *** Gab1[phosAb] phos(Y307)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+ 5+ 10+ min
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** source: 19401591-Fig-4b
  *** Gab1[phosAb] phos(Y627)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+ 5+ 10+ min
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** source: 19401591-Fig-4b
  *** Gab1[phosAb] phos(Y627)[phosAb] is increased irt Egf (tnr)
    *** cells: mEFs in BMLS
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** source: 19401591-Fig-4c

Rasa1--  ???

  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: VERO<xGab1> in BMLS
    *** unaffected by: xRasa1(R789Q/R903Q) [addition]
    *** source: 15574420-Fig-3d


Gab1 features

  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: xGab1(104-694)"PH-domain-mutant" [substitution]
    *** source: 11134009-Fig-7b
  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** enhanced by: xGab1(104-694)"PH-domain-mutant" [substitution]
    *** partially inhibited by: xGab1(Y627F) [substitution]
    *** source: 11134009-Fig-7d
  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: VERO<xGab1> in BMLS
    *** unaffected by: xGab1(Y627F) [substitution]
    *** inhibited by: xGab1(del(161-318)) [substitution]
    *** inhibited by: xGab1(del(161-318)/Y627F) [substitution]
    *** source: 15574420-Fig-2c
  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: VERO<xGab1> in BMLS
    *** unaffected by: xGab1(Y627F) [substitution]
    *** source: 15574420-Fig-3d

chemicals

  *** Gab1[Ab]IP Yphos[pYAb] is increased irt Egf (10 min)
    *** cells: MIMCD3 in BMLS
    *** inhibited by: U0126 [addition]
    *** source: 11896055(D)
  *** Gab1[Ab] Yphos[pYAb-ppt] is increased irt Egf (5 min)
    *** cells: HEK293 in BMLS
    *** unaffected by: 5Iodotubercidin [addition]
    *** unaffected by: U0126 [addition]
    *** source: 16687399-Fig-3b
  *** Gab1[Ab] Yphos[pYAb-ppt] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 1++ 5+ 10+ min
    *** partially inhibited by: Wortmannin [addition]
    *** source: 16687399-Fig-4a
  *** Gab1[Ab] Yphos[pYAb-ppt] is increased irt Egf (10 min)
    *** cells: MIMCD3 in BMLS
    *** inhibited by: U0126 [addition]
    *** source: 11896055(D)
  *** Gab1[Ab]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** partially inhibited by: Wortmannin [addition] no-bkg-control
    *** partially inhibited by: LY294002 [addition] no-bkg-control
    *** source: 11134009-Fig-6b
  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: Wortmannin [addition] no-bkg-control
    *** source: 11134009-Fig-7b

no extras

  *** Gab1[Ab]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: MDAMB468 in BMLS
    *** times: 0 5++ 10++ 30+ min
    *** source: 14665621-Fig-2a
  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: COS7 in BMLS
    *** times: 0 1++ 3++ 30+ min
    *** source: 11323411-Fig-7b
  *** Gab1[Ab]IP Yphos[pYAb] is increased irt Egf (4 min)
    *** cells: COS7 in BMLS
    *** source: 10788507(D)
  *** Gab1[Ab]IP Yphos[pYAb] is increased irt Egf (10 min)
    *** cells: PC12 in BMLS
    *** source: 11353842-Fig-2d
  *** Gab1[Ab]IP Yphos[pYAb] is increased irt Egf (10 min)
    *** cells: T47D in BMLS
    *** source: 15952937(D)
  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** source: 11134009-Fig-7d
  *** xGab1(116-695)[tAb]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: MDCK<xGab1(116-695)> in BMLS
    *** times: 0 1+ 3+ 15+ 30+ 60+ 120 min
    *** source: 10022866-Fig-3b
  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: MDCK<xGab1> in BMLS
    *** times: 0 1++ 3+ 15+ 30 60 120 min
    *** source: 10022866-Fig-3b
  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: MCF10A<xGab1> in BMLS
    *** times: 0 3++ 20+ min
    *** source: 19172738-Fig-1b


phos(Y307)

  *** xGab1[tAb]IP phos(Y307)[phosAb] is increased irt Egf (times)
    *** cells: VERO<xGab1> in BMLS
    *** times: 0 2+ 5+ min
    *** inhibited by: xGab1(Y627F) [substitution]
    *** source: 15574420-Fig-7a

phos(Y627)

  *** Gab1[phosAb] phos(Y627)[phosAb] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 1++ 5+ 10+ min
    *** partially inhibited by: Wortmannin [addition]
    *** source: 16687399-Fig-4a
  ------------------------------------------------------------
  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** partially inhibited by: xGab1(Y627F) [substitution]
    *** source: 11323411-Fig-1

phos(Y659)

  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** partially inhibited by: xGab1(Y659F) [substitution]
    *** source: 11323411-Fig-1

phos(Y627/Y659)

  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** partially inhibited by: xGab1(Y627F/Y659F) [substitution]
    *** source: 11323411-Fig-1

=========================
Coprecipitation with EgfR
=========================

Gnai1-- and/or Gnai3--

  *** Gab1[Ab] copptby[WB] EgfR[Ab] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+ 5+ min
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** source: 19401591-Fig-5a

  *** Gab1[Ab] copptby[WB] EgfR[Ab] is increased irt Egf (5 min)
    *** cells: HEK293 in BMLS
    *** source: 19401591-Fig-5d
  *** EgfR[Ab] copptby[WB] Gab1[Ab] is increased irt Egf (5 min)
    *** cells: HEK293 in BMLS
    *** source: 19401591-Fig-5d

=========================================================
Gab1 relocates to the Membrane Fraction in reponse to Egf
=========================================================

  *** Gab1[Ab] infraction(membrane)[WB] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 1+ 3+ min
    *** inhibited by: Wortmannin [addition]
    *** source: 16687399-Fig-4c
  *** xGab1[tAb] infraction(membrane)[WB] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: xGab1(104-694)"PH-domain-mutant" [substitution]
    *** inhibited by: Wortmannin [addition] no-bkg-control
    *** source: 11134009-Fig-7a
  *** xGab1[tAb] infraction(membrane)[WB] is increased irt Egf (5 min)
    *** cells: VERO<xGab1> in BMLS
    *** source: 15574420-Fig-3a
  *** xGab1[tAb] infraction(membrane)[WB] is increased irt Egf (5 min)
    *** cells: VERO<xGab1> in BMLS
    *** unaffected by: xGab1(Y627F) [substitution]
    *** unaffected by: xGab1(del(161-318)) [substitution]
    *** unaffected by: xGab1(del(161-318)/Y627F) [substitution]
    *** source: 15574420-Fig-2b
  *** xGab1[tAb] infraction(membrane)[WB] is increased irt Egf (5 min)
    *** cells: VERO<xGab1> in BMLS
    *** unaffected by: xGab1(Y627F) [substitution]
    *** unaffected by: xGab1(del(161-318)) [substitution]
    *** unaffected by: xGab1(del(161-318)/Y627F) [substitution]
    *** source: 15574420-Fig-4a
  *** xGab1(Y627F)[tAb] infraction(membrane)[WB] is detectable
    *** cells: VERO<xGab1(Y627F)> in BMLS "treated with Egf for 5 min"
    *** source: 15574420-Fig-3a

======================================================
Physical interactions with other EgfR complex proteins
======================================================

Gnai1--

  *** Gab1[Ab] copptby[WB] Gnai1[Ab] is increased irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** source: 19401591-Fig-5c

Gnai3--

  *** Gab1[Ab] copptby[WB] Gnai3[Ab] is increased irt Egf (5 min)
    *** cells: HEK293 in BMLS
    *** source: 19401591-Fig-5d
  *** Gab1[Ab] copptby[WB] Gnai3[Ab] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+ 5++ min
    *** source: 19401591-Fig-5a

Grb2--

  *** Gab1[Ab] copptby[WB] Grb2[Ab] is unchanged irt Egf (10 min)
    *** cells: MIMCD3 in BMLS
    *** times: 0+ 10+ min
    *** source: 11896055(D)
  *** Grb2[Ab] copptby[WB] Gab1[Ab] is increased irt Egf (10 min)
    *** cells: PC12 in BMLS
    *** times: 0+ 10++ min
    *** source: 11353842-Fig-2d

Pi3k--

  *** xGab1[tAb]IP IVLKA(PtdIns)[TLC] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** inhibited by: xGab1(Y447F/Y472F/Y589F)"Pi3k-binding-site-mut" [substitution]
    *** source: 10788507(D)
  *** Gab1[Ab] snaggedby[WB] rPik3r1[GST] is increased irt Egf (10 min)
    *** cells: MIMCD3 in BMLS
    *** inhibited by: U0126 [addition]
    *** source: 11896055(D)
  *** Gab1[Ab]IP IVLKA(PtdIns)[TLC] is increased irt Egf (10 min)
    *** cells: MIMCD3 in BMLS
    *** inhibited by: U0126 [addition]
    *** source: 11896055(D)
  *** Gab1[Ab] copptby[WB] Pik3rs[Ab] is increased irt Egf (tnr)
    *** cells: mEFs in BMLS
    *** source: 19401591-Fig-5d
  *** Gab1[Ab] snaggedby[WB] rPik3r1[GST] is increased irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** source: 15574420-Fig-4c
  *** Gab1[Ab] snaggedby[WB] rPik3r1[GST] is increased irt Egf (5 min)
    *** cells: mEFs<Shp2~null><xShp2(del(46-110))> in BMLS
    *** source: 15574420-Fig-4c
  *** xGab1[tAb] snaggedby[WB] rPik3r1[GST] is increased irt Egf (5 min)
    *** cells: VERO<xGab1> in BMLS
    *** enhanced by: xGab1(Y627F) [substitution]
    *** partially inhibited by: xGab1(Y447F/Y472F/Y619F) [substitution]
    *** enhanced by: xGab1(del(161-318)) [substitution]
    *** source: 15574420-Fig-4d

Rasa1--

  *** Gab1[Ab] snaggedby[WB] rRasa1(182-442)[GST] is increased irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** source: 15574420-Fig-4b
  *** Gab1[Ab] snaggedby[WB] rRasa1(182-442)[GST] is increased irt Egf (5 min)
    *** cells: mEFs<Shp2~null><xShp2(del(46-110))> in BMLS
    *** source: 15574420-Fig-4b
  *** Gab1[Ab] snaggedby[WB] rRasa1(351-442)[GST] is unchanged irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** source: 15574420-Fig-4b
  *** Gab1[Ab] snaggedby[WB] rRasa1(351-442)[GST] is unchanged irt Egf (5 min)
    *** cells: mEFs<Shp2~null><xShp2(del(46-110))> in BMLS
    *** source: 15574420-Fig-4b
  *** xGab1[tAb] snaggedby[WB] rRasa1(182-442)[GST] is detectable
    *** cells: VERO<xGab1> in BMLS "treated with Egf for 5 min"
    *** enhanced by: xGab1(Y627F) [substitution]
    *** enhanced by: xGab1(Y627F/Y242F) [substitution]
    *** enhanced by: xGab1(Y627F/Y317F) [substitution]
    *** source: 15574420-Fig-6a
  *** xGab1[tAb] snaggedby[WB] rRasa1(182-442)[GST] is detectable
    *** cells: VERO<xGab1> in BMLS "treated with Egf for 5 min"
    *** enhanced by: xGab1(Y627F) [substitution]
    *** inhibited by: xGab1(del(161-318)) [substitution]
    *** enhanced by: xGab1(Y242F/Y259F) [substitution]
    *** inhibited by: xGab1(Y307F/Y317F) [substitution]
    *** source: 15574420-Fig-6d
  *** xGab1[tAb] snaggedby[WB] rRasa1(182-442)[GST] is increased irt Egf (5 min)
    *** cells: VERO<xGab1> in BMLS
    *** enhanced by: xGab1(Y627F) [substitution]
    *** enhanced by: xGab1(Y242F/Y259F/Y627F) [substitution]
    *** inhibited by: xGab1(Y307F/Y317F/Y627F) [substitution]
    *** source: 15574420-Fig-6a
  *** xGab1[tAb] snaggedby[WB] rRasa1(182-442)[GST] is increased irt Egf (5 min)
    *** cells: VERO<xGab1> in BMLS
    *** enhanced by: xGab1(Y627F) [substitution]
    *** enhanced by: xGab1(Y447F/Y472F/Y619F) [substitution]
    *** inhibited by: xGab1(del(161-318)) [substitution]
    *** source: 15574420-Fig-4d

Shp2--

  *** Gab1[Ab] copptby[WB] Shp2[Ab] is increased irt Egf (10 min)
    *** cells: MIMCD3 in BMLS
    *** inhibited by: U0126 [addition]
    *** source: 11896055(D)
  *** Gab1[Ab] copptby[WB] Shp2[Ab] is increased irt Egf (5 min)
    *** cells: HEK293 in BMLS
    *** inhibited by: Wortmannin [addition]
    *** source: 16687399-Fig-4d
  *** Gab1[Ab] copptby[WB] Shp2[Ab] is increased irt Egf (5 min)
    *** cells: U87MG in BMLS
    *** source: 14701753-Fig-1a
  *** Gab1[Ab] copptby[WB] Shp2[Ab] is increased irt Egf (5 min)
    *** cells: U87MG in BMLS
    *** source: 14701753-Fig-8c
  *** Gab1[Ab] copptby[WB] xShp2[Ab] is increased irt Egf (5 min)
    *** cells: U87MG in BMLS
    *** partially inhibited by: xShp2(R32E)"N-SH2-domain-mut" [substitution]
    *** partially inhibited by: xShp2(R138E)"C-SH2-domain-mut" [substitution]
    *** source: 14701753-Fig-8c
  *** Gab1[Ab] copptby[WB] xShp2[Ab] is increased irt Egf (5 min)
    *** cells: U87MG in BMLS
    *** unaffected by: xShp2(C459S)"PTPase-mut" [substitution]
    *** source: 14701753-Fig-9a
  *** Gab1[Ab] copptby[WB] Shp2[Ab] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** partially inhibited by: Wortmannin [addition] no-bkg-control
    *** partially inhibited by: LY294002 [addition] no-bkg-control
    *** source: 11134009-Fig-6a
  *** xGab1(Y627F)[tAb] copptby[WB] xShp2(C459S)[Ab] is increased irt Egf (5 min)
    *** cells: VERO<xGab1(Y627F)><xShp2(C459S)> in BMLS
    *** inhibited by: xGab1(del(161-318)/Y627F) [substitution]
    *** source: 15574420-Fig-2d
  *** xGab1(Y627F)[tAb] copptby[WB] xShp2[Ab] is increased irt Egf (5 min)
    *** cells: VERO<xGab1(Y627F)><xShp2> in BMLS
    *** enhanced by: xShp2(C459S) [substitution]
    *** source: 15574420-Fig-1b
  *** xGab1(Y627F/Y447F/Y472F/Y619F)[tAb] copptby[WB] xShp2[Ab] is increased irt Egf (5 min)
    *** cells: VERO<xGab1(Y447F/Y472F/Y619F/Y627F)><xShp2> in BMLS
    *** enhanced by: xShp2(C459S) [substitution]
    *** source: 15574420-Fig-1b


