======================
Phosphorylation on S15
======================

Atm--

  *** Tp53[phosAb] phos(S15)[phosAb] is unchanged irt NCS (1 hr)
    *** cells: AT2RO<Atm~fmut><xMdm4> in BMS
    *** comment: no control run for total Tp53 protein
    *** source: 15788536-Fig-3b
  *** Tp53[phosAb] phos(S15)[phosAb] is unchanged irt NCS (15 min)
    *** cells: AT59<Atm~fmut> in BMS
    *** comment: no control run for total Tp53 protein
    *** source: 15788536-Fig-3a
  *** Tp53[phosAb] phos(S15)[phosAb] is unchanged irt NCS (15 min)
    *** cells: L3AT<Atm~fmut> in BMS
    *** comment: no control run for total Tp53 protein
    *** source: 15788536-Fig-3a
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt NCS (times)
    *** cells: L3AT<Atm~fmut> in BMS
    *** times: 0 90+ 150++ 210++ min
    *** comment: no control run for total Tp53 protein
    *** comment: L3AT has a small amount of ATM protein in Fig-3a
    *** source: 15788536-Fig-1a

Mdm4-- ???

  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt NCS (1 hr)
    *** cells: U2OS<xMdm4> in BMS
    *** unaffected by: xMdm4(S403A) [substitution]
    *** comment: no control run for total Tp53 protein
    *** source: 15788536-Fig-2a
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt NCS (1 hr)
    *** cells: U2OS<xMdm4> in BMS
    *** unaffected by: xMdm4(S403A) [substitution]
    *** comment: no control run for total Tp53 protein
    *** source: 15788536-Fig-2b

not Trim28--

  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt NCS (30 min)
    *** cells: U2OS<Trim28~kd><xTrim28> in BMS
    *** does not req: xTrim28 [omission]
    *** unaffected by: xTrim28(S824A) [substitution]
    *** unaffected by: xTrim28(S824D) [substitution]
    *** source: 16862143-Fig-S5b

chemicals

  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt NCS (3 hr)
    *** cells: MCF7<xMdm4><xMdm2> in BMS "pretreated with MG132"
    *** inhibited by: KU55933 [pretreatment]
    *** source: 15788536-Fig-1d
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt NCS (times)
    *** cells: MCF7 in BMS
    *** times: 0 1+ 2++ 3+ 5 hr
    *** inhibited by: KU55933 [pretreatment]
    *** comment: no control run for total Tp53 protein
    *** source: 15788536-Fig-1c
  *** xTp53[phosAb] phos(S15)[phosAb] is increased irt NCS (15 min)
    *** cells: U2OS<xMdm4> in BMS
    *** partially inhibited by: Wortmannin [pretreatment]
    *** inhibited by: KU55933 [pretreatment]
    *** comment: no control run for total Tp53 protein
    *** source: 15788536-Fig-9

no extras

  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt NCS (1 hr)
    *** cells: VH25<xMdm4> in BMS
    *** comment: no control run for total Tp53 protein
    *** source: 15788536-Fig-3b
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt NCS (15 min)
    *** cells: C3ABR in BMS
    *** comment: no control run for total Tp53 protein
    *** source: 15788536-Fig-3a
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt NCS (times)
    *** cells: C3ABR in BMS
    *** times: 0 90+ 150+ 210+ min
    *** comment: no control run for total Tp53 protein
    *** source: 15788536-Fig-1a
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt NCS (times)
    *** cells: MCF7<xMdm4> in BMS
    *** times: 0 5+ 7++ hr
    *** comment: no control run for total Tp53 protein
    *** source: 15788536-Fig-1b
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt NCS (30 min)
    *** cells: U2OS in BMS
    *** source: 16862143-Fig-S5b



