UniProt summary:
Sumoylated on Lys-277 and Lys-309 with SUMO1;
the modification results in phosphorylation of Ser-85 by ATM
leading to a replacement of the sumoylation by mono-ubiquitination
on these residues. 14651848

===========
Sumoylation
===========

Atm--

  *** xNemo[tAb]IP sumo[tSuAb] is increased irt Etoposide (1 hr)
    *** cells: HEK293<xNemo><xSumo1> in BMS
    *** partially reqs: Atm [RNAi]
    *** source: 14651848-Fig-6b

Chek1--

  *** xNemo[tAb]IP sumo[tSuAb] is increased irt Etoposide (1 hr)
    *** cells: HEK293<xNemo><xSumo1> in BMS
    *** partially reqs: Chek1 [RNAi]
    *** source: 14651848-Fig-6b

Nemo Features

  requires Nemo sites C417 K277 K309

  *** xNemo[tAb] sumo[SuAb-ppt] is increased irt Etoposide (1 hr)
    *** cells: 70Z3<Nemo~null><xNemo> in BMS
    *** inhibited by: xNemo(C417R) [substitution]
    *** comment: Sumo-Nemo is a single band
    *** source: 14651848-Fig-1a
  *** xNemo[tAb]IP sumo[SuAb] is increased irt Etoposide (1 hr)
    *** cells: 70Z3<Nemo~null><xNemo> in BMS
    *** inhibited by: xNemo(C417R) [substitution]
    *** comment: Sumo-Nemo is a single band
    *** source: 14651848-Fig-1a
  *** xNemo[tAb]IP sumo[SuAb] is increased irt Etoposide (1 hr)
    *** cells: 70Z3<Nemo~null><xNemo> in BMS
    *** inhibited by: xNemo(C417R) [substitution]
    *** inhibited by: xNemo(K277A/K309A) [substitution]
    *** source: 14651848-Fig-2b

  does not req S85 which is required for ubiq

  *** xNemo[tAb]IP sumo[SuAb] is increased irt Etoposide (1 hr)
    *** cells: 70Z3<Nemo~null><xNemo> in BMS
    *** unaffected by: xNemo(S85A) [substitution]
    *** source: 16497931-Fig-S3a

  does not require binding to IKK comples

  *** xNemo[tAb]IP sumo[SuAb] is increased irt Etoposide (1 hr)
    *** cells: 70Z3<Nemo~null><xNemo> in BMS
    *** unaffected by: NBDpeptide [pretreatment]
    *** source: 14651848-Fig-4c

Timing is transient around 60 min

  *** xNemo[tAb]IP sumo[tSuAb] is increased irt Etoposide (times)
    *** cells: HEK293<xNemo><xSumo1> in BMS
    *** times: 0 30 60+ 90 120 min
    *** comment: Sumo-Nemo is a single band
    *** comment: Sumoylation precedes Ubiquitination by 30 min
    *** source: 14651848-Fig-5a
  *** xNemo[tAb]IP sumo[SuAb] is increased irt Etoposide (times)
    *** cells: 70Z3<Nemo~null><xNemo> in BMS
    *** times: 0 30 45+ 60++ 90 120 min
    *** source: 14651848-Fig-5a


no extras

  *** Nemo[Ab]IP sumo[SuAb] is increased irt Etoposide (1 hr)
    *** cells: HEK293 in BMS
    *** comment: signal is very weak but appears to be real
    *** source: 14651848-Fig-1c

=====================
Relocation to Nucleus
=====================

Question: does Nemo require Sumoylation to relocate to nucleus or
does it relocate to nucleus and then get Sumoylated?

requires Nemo sites K277 K309 which are also required for Sumo

  *** xNemo[tAb] locatedin(nucleus)[IHC] is increased irt Etoposide (90 min)
    *** cells: 70Z3<Nemo~null><xNemo> in BMS
    *** inhibited by: xNemo(K277A/K309A) [substitution]
    *** source: 14651848-Fig-3a

  *** xNemo[tAb] infraction(nuclear)[WB] is increased irt Etoposide (times)
    *** cells: 70Z3<Nemo~null><xNemo> in BMS
    *** times: 0+ 60++ 90++ 120++ min
    *** source: 14651848-Fig-3c

Timing is transient around 2 hrs

  *** xNemo[tAb] locatedin(nucleus)[IHC] is increased irt Etoposide (times)
    *** cells: 70Z3<Nemo~null><xNemo> in BMS
    *** times: 0 1+ 2++ 3+ 4+ hr
    *** unaffected by: xNemo(ubiq/S85A) [substitution]
    *** comment: substitution with xNemo(S85A) prevents relocation to cyto at 3-4 hrs
    *** source: 16497931-Fig-4a
  *** xNemo[tAb] locatedin(nucleus)[IHC] is increased irt Etoposide (times)
    *** cells: 70Z3<Nemo~null><xNemo> in BMS
    *** times: 0 60+ 120++ 180+ min
    *** comment: substitution with xNemo(S85A) prevents relocation to cyto at 180 min
    *** source: 16497931-Fig-S4d
  *** Nemo[Ab] infraction(nuclear)[WB] is increased irt Etoposide (times)
    *** cells: HEK293 in BMS
    *** times: 0 1+ 2+ 3 hr
    *** source: 16497931-Fig-4d
