Hypothesis from 16959574 is that Tsc2 is first primed by phos on S1387 by Ampk
followed by phos on S1383 by Gsk3b

Ampk => Tsc2 phos(S1271)

  *** rTsc2(1237-1291)[32P] phos[32P-ATP] is increased by xAmpka1[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xAmpka1> in BMS
    *** inhibited by: rTsc2(S1271A) [substitution]
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 14651849-Fig-S4a

Ampk => Tsc2 phos(S1387)

  *** rTsc2(1342-1409)[32P] phos[32P-ATP] is increased by xAmpka1[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xAmpka1> in BMS
    *** inhibited by: rTsc2(S1387A) [substitution]
    *** inhibited by: rTsc2(S1387D) [substitution]
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 14651849-Fig-5c
  *** rTsc2(1342-1409)[32P] phos[32P-ATP] is increased by xAmpka1[Ab]IP
    *** cells: none
    *** IPfrom: HEK293<xAmpka1> in BMS "treated with 2DOG for 30 min"
    *** inhibited by: rTsc2(1342-1409/S1387A) [substitution]
    *** unaffected by: rTsc2(1342-1409/S1383A/S1379A) [substitution]
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 16959574-Fig-S6a

Gsk3b => Tsc2 phos(S1387)

  *** rTsc2(1342-1409)[32P] phos[32P-ATP] is increased by xGsk3b(S9A)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xGsk3b(S9A)"CA"> in BMS
    *** comment: requires prephos by xAmpka1[Ab]IP from HEK293<xAmpka1> in BMS "treated with 2DOG for 30 min"
    *** partially inhibited by: xAmpka1(mnr)"KD" [substitution]
    *** partially inhibited by: xGsk3b(mnr)"KD" [substitution]
    *** inhibited by: rTsc2(1342-1409/S1387A) [substitution]
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 16959574-Fig-6b

Gsk3b => Tsc2 phos(S1387) phos(S1383/S1379)

  *** rTsc2(1342-1409)[32P] phos[32P-ATP] is increased by xGsk3b(S9A)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xGsk3b(S9A)"CA"> in BMS
    *** comment: requires prephos by xAmpka1[Ab]IP from HEK293<xAmpka1> in BMS "treated with 2DOG for 30 min"
    *** inhibited by: rTsc2(1342-1409/S1387A) [substitution]
    *** inhibited by: rTsc2(1342-1409/S1383A/S1379A) [substitution]
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 16959574-Fig-S6a

  *** xTsc2[phosAb] phos(S1383/S1379)[phosAb] is increased itpo xGsk3b(S9A)"CA"
    *** cells: HEK293<xTsc2> in BMS
    *** enhanced by: 2DOG [addition]
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 16959574-Fig-6d
  *** xTsc2[phosAb] phos(S1383/S1379)[phosAb] is increased itpo xGsk3b(S9A)"CA"
    *** cells: HEK293<xTsc2> in BMS
    *** inhibited by: Compound-C [addition]
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 16959574-Fig-6d
  *** xTsc2[tAb]IP phos(S1383/S1379)[phosAb] is increased itpo xGsk3b
    *** cells: HEK293<xTsc2> in BMS
    *** inhibited by: xTsc2(S1383A/S1379A) [substitution]
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 16959574-Fig-S6g

  *** xTsc2[phosAb] phos(S1383/S1379)[phosAb] is increased itpo xGsk3b(S9A)"CA"
    *** cells: HEK293<xTsc2> in BMS
    *** inhibited by: xTsc2(S1387A) [substitution]
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 16959574-Fig-6d

Gsk3b => Tsc2 phos(S1387) not phos(S1371) not phos(S1375)  not phos(S1379)

  *** rTsc2(1342-1409)[32P] phos[32P-ATP] is increased by xGsk3b(S9A)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xGsk3b(S9A)"CA"> in BMS
    *** comment: requires prephos by xAmpka1[Ab]IP from HEK293<xAmpka1> in BMS "treated with 2DOG for 30 min"
    *** unaffected by: rTsc2(1342-1409/S1371A) [substitution]
    *** unaffected by: rTsc2(1342-1409/S1375A) [substitution]
    *** unaffected by: rTsc2(1342-1409/S1379A) [substitution]
    *** inhibited by: rTsc2(1342-1409/S1383A) [substitution]
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 16959574-Fig-S6b








  *** rTsc2(1342-1409)[32P] phos[32P-ATP] is increased by xAmpka1[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xAmpka1> in BMS
    *** inhibited by: xAmpka1(D159A)"KD" [substitution]
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 14651849-Fig-5c

