===================================================
Tsc2 Phosphorylation sites required for interaction
===================================================

phos(S1254) (aka S1210)

  *** xYwhab[tAb] copptby[WB] xTsc2[tAb] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xTsc2(S1254A) [addition]
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 12582162-Fig-5c
  *** xYwhab[tAb] copptby[WB] xTsc2[tAb] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xTsc2(S1254A) [substitution]
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 12582162-Fig-2c
  *** xTsc2[Ab] copptby[WB] xYwhab[tAb] is detectable
    *** cells: HEK293T<xTsc2><xYwhab> in BMS
    *** unaffected by: xTsc2(S540A) [substitution]
    *** unaffected by: xTsc2(S1387A) [substitution]
    *** inhibited by: xTsc2(S1254A) [substitution] 
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 12468542-Fig-3c

phos(S939A/T1462A) not S1254 ??? outvoted

  *** xTsc2[tAb] copptby[WB] Ywhas[Ab] is detectable
    *** cells: mEFs<Tsc2~null><xTsc2> in BMS
    *** inhibited by: xTsc2(S939A) [substitution]
    *** inhibited by: xTsc2(T1462A) [substitution]
    *** inhibited by: xTsc2(S939A/T1462A) [substitution]
    *** unaffected by: xTsc2(S1254A) [substitution]
    *** source: 18198340-Fig-3a
  *** xTsc2[tAb] copptby[WB] Ywhas[Ab] is detectable
    *** cells: mEFs<Tsc2~null><xTsc2> in BMS
    *** inhibited by: xTsc2(S939A/T1462A) [substitution]
    *** source: 18198340-Fig-3c

other phos

  *** xTsc2[Ab] copptby[WB] xYwhab[tAb] is detectable
    *** cells: HEK293T<xTsc2><xYwhab> in BMS
    *** unaffected by: xTsc2(S939A) [substitution]
    *** unaffected by: xTsc2(S1130A) [substitution]
    *** unaffected by: xTsc2(S1132A) [substitution]
    *** unaffected by: xTsc2(S1464A) [substitution]
    *** inhibited by: xTsc2(S939A/S1130A/S1132A/S1464A) [substitution] 
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 12468542-Fig-3a
  *** xTsc2[Ab] snaggedby[WB] rYwhab[GST] is detectable
    *** cells: HEK293T<xTsc2> in BMS
    *** comment: inhibited by treatment of lysates with calf intestinal PPase
    *** source: 12468542-Fig-2c

=================
Required Proteins
=================

Mapkapk2--

  *** xYwhab[tAb] copptby[WB] xTsc2[tAb] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMapkapk2(mnr)"DN" [addition]
    *** source: 12582162-Fig-3a
  *** xYwhab[tAb] copptby[WB] xTsc2[tAb] is increased itpo xMapkapk2
    *** cells: HEK293 in BMS
    *** enhanced by: xMapkapk2(mnr)"CA" [substitution]
    *** source: 12582162-Fig-3a

not Mapkapk5--

  *** xYwhab[tAb] copptby[WB] xTsc2[tAb] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: xMapkapk5 [addition]
    *** unaffected by: xMapkapk5(T182D)"CA" [addition]
    *** unaffected by: xMapkapk5(mnr)"DN" [addition]
    *** source: 12582162-Fig-3b

not Mnk1--

  *** xYwhab[tAb] copptby[WB] xTsc2[tAb] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: xMnk1 [addition]
    *** unaffected by: xMnk1(mnr)"CA" [addition]
    *** unaffected by: xMnk1(mnr)"DN" [addition]
    *** source: 12582162-Fig-3c

not Tsc1--

  *** Ywhab[Ab] copptby[WB] xTsc2[tAb] is detectable
    *** cells: HEK293<xTsc1><xTsc2> in BMS
    *** does not req: xTsc1 [omission]
    *** source: 12364343-Fig-2b
  *** xTsc2[tAb] copptby[WB] xYwhab[tAb] is detectable
    *** cells: HEK293<xTsc1><xTsc2><xYwhab> in BMS
    *** does not req: xTsc1 [omission]
    *** source: 12364343-Fig-2a



itpos

  *** xYwhab[tAb] copptby[WB] xTsc2[tAb] is increased itpo (xP38? + xMkk3(mnr)"CA")
    *** cells: HEK293 in BMS
    *** source: 12582162-Fig-2a
  *** xYwhab[tAb] copptby[WB] xTsc2[tAb] is increased itpo (xP38? + xMkk3(mnr)"CA")
    *** cells: HEK293 in BMS
    *** source: 12582162-Fig-4b
  *** Tsc2[tAb] copptby[WB] Ywhas[Ab] is decreased itpo xDdit4
    *** cells: U2OS in BMS
    *** reversed by: xDdit4(mnr)"RPAA" [substitution]
    *** source: 18198340-Fig-4b


chemicals

  *** Tsc2[Ab] copptby[WB] Ywhas[Ab] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: SB203580 [addition]
    *** source: 12582162-Fig-1b
  *** xYwhab[tAb] copptby[WB] xTsc2[tAb] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: SB203580 [addition]
    *** unaffected by: PD98059 [addition]
    *** unaffected by: Wortmannin [addition]
    *** source: 12582162-Fig-1a
  *** Tsc2[Ab] snaggedby[WB] rYwhab[GST] is detectable
    *** cells: HEK293T<xDdit4> in BMS
    *** comment: inhibited by addition of peptide PHCVPRDLSWLDLEANMCLP
    *** source: 18198340-Fig-5b


no extras

  *** Tsc2[Ab] snaggedby[WB] rYwhab[GST] is detectable
    *** cells: NIH3T3 in BMLS "stimulated with serum for 60 min"
    *** source: 12438239-Fig-5a
  *** Ywhab[Ab] copptby[WB] xTsc2[tAb] is detectable
    *** cells: HEK293<xTsc1><xTsc2> in BMS
    *** source: 12364343-Fig-1b

=======================
Semi Direct Association
=======================

  *** xTsc2[tAb]IP boundby[WB] xYwhab[tAb]IP is detectable
    *** cells: none
    *** IP1from: HEK293<xTsc2> in BMS
    *** IP2from: HEK293<xYwhab> in BMS
    *** inhibited by: xYwhab(mnr)"DN" [substitution]
    *** comment: inhibited by treating xTsc2[tAb]IP with lambda PPase
    *** source: 12364343-Fig-4a

=============================================
Ywhab features required for Tsc2 association
=============================================

  *** xTsc2[tAb] copptby[WB] xYwhab[tAb] is detectable
    *** cells: HEK293<xTsc1><xTsc2><xYwhab> in BMS
    *** inhibited by: xYwhab(mnr)"DN" [substitution]
    *** source: 12364343-Fig-2a
  *** xYwhab[tAb] copptby[WB] xTsc2[tAb] is detectable
    *** cells: HEK293<xTsc1><xTsc2><xYwhab> in BMS
    *** inhibited by: xYwhab(mnr)"DN" [substitution]
    *** source: 12364343-Fig-1a
  *** xTsc2[Ab] copptby[WB] xYwhab[tAb] is detectable
    *** cells: U2OS<xTsc2><xYwhab> in BMS
    *** inhibited by: xYwhab(R58A) [substitution]
    *** inhibited by: xYwhab(R62A) [substitution]
    *** source: 12468542-Fig-2a
  *** xTsc2[Ab] snaggedby[WB] rYwhab[GST] is detectable
    *** cells: HEK293T<xTsc2> in BMS
    *** inhibited by: xYwhab(R58A/R62A) [substitution]
    *** source: 12468542-Fig-2c

===========================================
Tsc2 regions required for Ywhab association
===========================================

  *** Ywhab[Ab] copptby[WB] xTsc2[tAb] is detectable
    *** cells: HEK293<xTsc2> in BMS
    *** inhibited by: xTsc2(1-608) [substitution]
    *** inhibited by: xTsc2(1-1080) [substitution]
    *** inhibited by: xTsc2(1-1200) [substitution]
    *** unaffected by: xTsc2(1-1320) [substitution]
    *** unaffected by: xTsc2(1101-1320) [substitution]
    *** unaffected by: xTsc2(1081-1765) [substitution]
    *** inhibited by: xTsc2(1321-1765) [substitution]
    *** comment: aa numbers NOT converted to Uniprot human canonical sequence
    *** source: 12364343-Fig-3a

  *** Ywhab[Ab] copptby[WB] xTsc2[tAb] is detectable
    *** cells: HEK293<xTsc2> in BMS
    *** inhibited by: xTsc2(S1210A) [substitution]
    *** unaffected by: xTsc2(S1297A) [substitution]
    *** unaffected by: xTsc2(S1345A) [substitution]
    *** unaffected by: xTsc2(S1688A) [substitution]
    *** inhibited by: xTsc2(S1210A/S1297A) [substitution]
    *** inhibited by: xTsc2(S1210A/S1688A) [substitution]
    *** unaffected by: xTsc2(S1345A/S1297A) [substitution]
    *** unaffected by: xTsc2(S1688A/S1297A) [substitution]
    *** unaffected by: xTsc2(S1688A/S1297A) [substitution]
    *** unaffected by: xTsc2(S939A/S1086A/S1088A/S1378A/S1422A/S1756A) [substitution]
    *** inhibited by: xTsc2(1101-1320/S1210A) [substitution]
    *** unaffected by: xTsc2(1101-1320) [substitution]
    *** comment: aa numbers NOT converted to Uniprot human canonical sequence
    *** source: 12364343-Fig-3a


=================================================
Tsc1 is also in the complex but it requires Tsc2
=================================================

  *** xTsc1[tAb] copptby[WB] xYwhab[tAb] is detectable
    *** cells: HEK293<xTsc1><xTsc2><xYwhab> in BMS
    *** reqs: xTsc2 [omission]
    *** source: 12364343-Fig-2a
  *** Ywhab[Ab] copptby[WB] xTsc1[tAb] is detectable
    *** cells: HEK293<xTsc1><xTsc2> in BMS
    *** reqs: xTsc2 [omission]
    *** source: 12364343-Fig-2b

=================================
Other Ywhas also can bind to Tsc2
=================================

  *** Ywhag[Ab] copptby[WB] xTsc2[tAb] is detectable
    *** cells: HEK293<xTsc1><xTsc2> in BMS
    *** source: 12364343-Fig-1b
  *** Ywhaq[Ab] copptby[WB] xTsc2[tAb] is detectable
    *** cells: HEK293<xTsc1><xTsc2> in BMS
    *** source: 12364343-Fig-1b
  *** Ywhaz[Ab] copptby[WB] xTsc2[tAb] is detectable
    *** cells: HEK293<xTsc1><xTsc2> in BMS
    *** source: 12364343-Fig-1b









