=====================
Direct Ubiquitination
=====================

  *** rTp53[35S] upshift[WBMS] is increased by (rUbe1 + rUbe2d2 + rUbiq + rPirh2)
    *** cells: none
    *** source: 12654245-Fig-6f
  *** rTp53[Ab] upshift[WBMS] is increased by (rUbe1 + rUbe2d2 + rUbiq + rPirh2)
    *** cells: none
    *** source: 12654245-Fig-6h

=================================
Ubiquitination in the presence of
=================================

  *** xTp53[Ab]IP ubiq[tUbAb] is increased itpo xPirh2
    *** cells: mEFs<Tp53~null><Mdm2~null><xTp53><xUbiq> in BMS
    *** source: 12654245-Fig-6c
  *** xTp53[Ab]IP ubiq[tUbAb] is increased itpo xPirh2
    *** cells: SAOS2<Tp53~null><xTp53><xUbiq> in BMS
    *** source: 12654245-Fig-6b
  *** xTp53[Ab]IP upshift[WBMS] is increased itpo xPirh2
    *** cells: SAOS2<Tp53~null><xTp53><xUbiq> in BMS
    *** source: 12654245-Fig-6b

===============================================
Decreased protein expression in the presence of
===============================================

  *** Tp53[Ab] prot-exp[WB] is decreased itpo xPirh2
    *** cells: MCF7 in BMS
    *** reversed by: xPirh2(H169A) [substitution]
    *** reversed by: xPirh2(C164A) [substitution]
    *** reversed by: xPirh2(del(145-186)) [substitution]
    *** source: 12654245-Fig-5c
  *** Tp53[Ab] prot-exp[WB] is decreased itpo xPirh2
    *** cells: MCF7 in BMS
    *** source: 12654245-Fig-5a
  *** Tp53[Ab] prot-exp[WB] is increased itao Pirh2 [AntiSense]
    *** cells: BJT in BMS
    *** source: 12654245-Fig-5b

==============================================
Increased protein expression in the absence of
==============================================

  *** Tp53[Ab] prot-exp[WB] is increased itao Pirh2 [RNAi]
    *** cells: BJ in BMS
    *** source: 15103385-Fig-4c
  *** Tp53[Ab] prot-exp[WB] is increased itao Pirh2 [RNAi]
    *** cells: U2OS in BMS
    *** source: 15989956-Fig-8a

==============================================
Decreased protein stability in the presence of
==============================================

  *** xTp53[Ab]IP prot-stability[35S-pulse-chase] is decreased itpo xPirh2
    *** cells: mEFs<Tp53~null><Mdm2~null><xTp53> in BMS
    *** source: 12654245-Fig-6e

=============================================
Increased protein stability in the absence of
=============================================

  *** Tp53[Ab]IP prot-stability[35S-pulse-chase] is increased itao Pirh2 [RNAi]
    *** cells: U2OS in BMS
    *** source: 15103385-Fig-4a

====================
Protein Association
====================

  *** Pirh2[Ab] copptby[WB] Tp53[Ab] is detectable
    *** cells: BJT in BMS
    *** source: 12654245-Fig-3f
  *** xTp53[35S] copptby[WB] Pirh2[Ab] is detectable
    *** cells: SAOS2 in BMS
    *** source: 12654245-Fig-3c
  *** xTp53[Ab] copptby[WB] xPirh2[Ab] is detectable
    *** cells: SAOS2<xTp53> in BMS
    *** inhibited by: xPirh2(C164A) [substitution]
    *** unaffected by: xPirh2(H169A) [substitution]
    *** inhibited by: xPirh2(del(145-186)) [substitution]
    *** source: 12654245-Fig-3d
  *** xTp53[Ab] snaggedby[WB] rPirh2[GST] is detectable
    *** cells: SAOS2<xTp53> in BMS
    *** source: 12654245-Fig-3e
  *** Tp53[Ab] copptby[WB] Pirh2[Ab] is detectable
    *** cells: BJT in BMS
    *** source: 12654245-Fig-3f


=================
Downstream Events
=================

  *** Tp53-reporter[Luc] is decreased itpo xPirh2
    *** cells: MCF7 in BMS
    *** unaffected by: xPirh2(del(145-186)) [substitution]
    *** source: 12654245-Fig-7b
  *** Tp53-reporter[Luc] is decreased itpo xPirh2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** unaffected by: xPirh2(del(145-186)) [substitution]
    *** source: 12654245-Fig-7a
