==============================================
Direct ubiquitination can use Ube2d1 or Ube2d2
==============================================

required Tp53 features

  *** rTp53[Ab] upshift[WBMS] is increased by (rUbe1 + rUbe2d1 + rUbiq + rMdm2)
    *** cells: none
    *** inhibited by: rTp53(1-369) [substitution]
    *** inhibited by: rTp53(1-350) [substitution]
    *** inhibited by: rTp53(1-326) [substitution]
    *** inhibited by: rTp53(1-289) [substitution]
    *** inhibited by: rTp53(1-160) [substitution]
    *** source: 9878046-Fig-2b
  *** rTp53[Ab] upshift[WBMS] is increased by (rUbe1 + rUbe2d1 + rUbiq + rMdm2)
    *** cells: none
    *** inhibited by: rTp53(L14Q/F19S) [substitution]
    *** inhibited by: rTp53(L22Q/W23S) [substitution]
    *** unaffected by: rTp53(R249A) [substitution]
    *** unaffected by: rTp53(R249S) [substitution]
    *** source: 9878046-Fig-2d
  *** rTp53[Ab] upshift[WBMS] is increased by (rUbe1 + rUbe2d1 + rUbiq + rMdm2)
    *** cells: none
    *** partially inhibited by: xTp53(K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** source: 11046142-Fig-6c
  *** rTp53[35S] upshift[WBMS] is increased by (rMdm2 + rUbe2d2 + rUbe1 + rUbiq)
    *** cells: none
    *** inhibited by: rTp53(K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** unaffected by: rTp53(K319A/K320A/K321A) [substitution]
    *** source: 17110336-Fig-2b
  *** rTp53[Ab] upshift[WBMS] is increased by (rMdm2 + rUbe2d2 + rUbe1 + rUbiq)
    *** cells: none
    *** inhibited by: rTp53(K370I/K372I/K373I/K381I/K382I/K386I) [substitution]
    *** source: 11713287-Fig-1b

required Mdm2 features

  *** rTp53[biotinUb] upshift[WBMS] is increased by (rUbe1 + rUbe2d1 + rUbiq + rMdm2)
    *** cells: none
    *** comment: ubiquitination reaction contains SF9 and HELA lysates
    *** inhibited by: rMdm2(C464A) [substitution]
    *** source: 9450543-Fig-4

Cdkn2a--

  *** rTp53[biotinUb] upshift[WBMS] is increased by (rUbe1 + rUbe2d1 + rUbiq + rMdm2)
    *** cells: none
    *** reqs: rMdm2 [omission]
    *** inhibited by: rCdkn2a [addition]
    *** source: 9878046-Fig-4b
  *** rTp53[Ab] upshift[WBMS] is increased by (rUbe1 + rUbe2d1 + rUbiq + rMdm2)
    *** cells: none
    *** partially reqs: rMdm2 [omission]
    *** partially inhibited by: rCdkn2a [addition]
    *** source: 9878046-Fig-4a

Csnk1a1--

  *** rTp53[biotinUb] upshift[WBMS] is increased by (rUbe1 + rUbe2d1 + rUbiq + rMdm2)
    *** cells: none
    *** comment: ubiq unaffected by pre-phos with rCsnk1a1
    *** source: 9878046-Fig-3d

Dnapk--

  *** rTp53[biotinUb] upshift[WBMS] is increased by (rUbe1 + rUbe2d1 + rUbiq + rMdm2)
    *** cells: none
    *** comment: ubiq partially inhib by pre-phos with pDnapk
    *** source: 9878046-Fig-3b
  *** rTp53[biotinUb] upshift[WBMS] is increased by (rUbe1 + rUbe2d1 + rUbiq + rMdm2)
    *** cells: none
    *** comment: ubiq partially inhib by pre-phos with pDnapk
    *** source: 9878046-Fig-3d

not Cdc34--

  *** rTp53[biotinUb] upshift[WBMS] is unchanged by (rUbe1 + rCdc34 + rUbiq + rMdm2)
    *** cells: none
    *** comment: ubiquitination reaction contains SF9 and HELA lysates
    *** source: 9450543-Fig-1

not Trim23--

  *** rTp53[biotinUb] upshift[WBMS] is unchanged by (rUbe1 + rTrim25 + rUbiq + rMdm2)
    *** cells: none
    *** comment: ubiquitination reaction contains SF9 and HELA lysates
    *** source: 9450543-Fig-1

not Ube2c--

  *** rTp53[biotinUb] upshift[WBMS] is unchanged by (rUbe1 + rUbe2c + rUbiq + rMdm2)
    *** cells: none
    *** comment: ubiquitination reaction contains SF9 and HELA lysates
    *** source: 9450543-Fig-1

not Ube2l3--

  *** rTp53[biotinUb] upshift[WBMS] is unchanged by (rUbe1 + rUbe2l3 + rUbiq + rMdm2)
    *** cells: none
    *** comment: ubiquitination reaction contains SF9 and HELA lysates
    *** source: 9450543-Fig-1

other

  *** rTp53[biotinUb] upshift[WBMS] is increased by (rUbe1 + rUbe2d1 + rUbiq + rMdm2)
    *** cells: none
    *** comment: ubiquitination reaction contains SF9 and HELA lysates
    *** source: 9450543-Fig-1
  *** rTp53[biotinUb] upshift[WBMS] is increased by (rUbe1 + rUbe2d1 + rUbiq + rMdm2)
    *** cells: none
    *** comment: ubiquitination reaction contains SF9 lysate
    *** source: 9450543-Fig-2a
  *** rTp53[Ab] upshift[WBMS] is increased by (rUbe1 + rUbe2d1 + rMdm2 + rUbiq)
    *** cells: none
    *** comment: at high Mdm2:Tp53 ratios the ubiq is poly and Tp53-FL decreases
    *** comment: at high Mdm2:Tp53 ratios substitution with rUbiq(dig)"no-lysines" produces only monoubiq and stable Tp53FL
    *** source: 14671306-Fig-1b
  *** rTp53[Ab] upshift[WBMS] is increased by (rUbe1 + rUbe2d1 + rMdm2 + rUbiq)
    *** cells: none
    *** source: 14671306-Fig-1a
  *** rTp53[Ab] upshift[WBMS] is increased by (rUbe1 + rUbe2d1 + rMdm2 + rUbiq)
    *** cells: none
    *** unaffected by: rUbiq(dig)"no-lysines" [substitution]
    *** comment: equal ratios of Mdm2:Tp53 were used
    *** source: 14671306-Fig-S1b
  *** rTp53[35S] upshift[WBMS] is increased by (rMdm2 + rUbe2d2 + rUbe1 + rUbiq)
    *** cells: none
    *** source: 17110336-Fig-3e
  *** rTp53[Ab] upshift[WBMS] is increased by (rUbe1 + rUbe2d1 + rUbiq + rMdm2)
    *** cells: none
    *** reqs: rMdm2 [omission]
    *** source: 9878046-Fig-1
  *** rTp53[biotinUb] upshift[WBMS] is increased by (rUbe1 + rUbe2d1 + rUbiq + rMdm2)
    *** cells: none
    *** reqs: rMdm2 [omission]
    *** source: 9878046-Fig-1
  *** rTp53[Ab] upshift[WBMS] is increased by (rMdm2 + rUbe2d2 + rUbe1 + rUbiq)
    *** cells: none
    *** source: 11713287-Fig-1a

=================================
Ubiquitination in the presence of
=================================

required Tp53 features

  *** xTp53[Ab] upshift[WBMS] is increased itpo xMdm2
    *** cells: mEFs<Tp53~null><Mdm2~null><xTp53> in BMS "treated with MG132"
    *** unaffected by: xTp53(K386R) [substitution]
    *** unaffected by: xTp53(K370R/K372R/K373R) [substitution]
    *** unaffected by: xTp53(K381R/K382R/K386R) [substitution]
    *** inhibited by: xTp53(K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** source: 11046142-Fig-6b
  *** xTp53[Ab] ubiq[tUbAb-ppt] is increased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53><xUbiq> in BMS
    *** unaffected by: xTp53(K370R/K372R/K373R) [substitution]
    *** unaffected by: xTp53(K381R/K382R/K386R) [substitution]
    *** inhibited by: xTp53(K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** source: 15242646-Fig-4a
  *** xTp53[Ab] ubiq[tUbAb-ppt] is increased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53><xUbiq> in BMS
    *** inhibited by: xTp53(F19A) [substitution]
    *** source: 15242646-Fig-4a
  *** xTp53[Ab] ubiq[tUbAb-ppt] is increased itpo xMdm2
    *** cells: CHO<xTp53><xUbiq> in BMS
    *** does not req: Nae1 [null-mut]
    *** inhibited by: xTp53(F19A) [substitution]
    *** source: 15242646-Fig-5a
  *** xTp53[Ab]IP ubiq[tUbAb] is increased itpo xMdm2
    *** cells: LFS041<Tp53~null><xTp53><xUbiq> in BMS "treated with MG132"
    *** enhanced by: xTp53(V143A) [substitution]
    *** enhanced by: xTp53(R173H) [substitution]
    *** enhanced by: xTp53(R248W) [substitution]
    *** source: 11340074-Fig-2c
  *** xTp53(V143A)[Ab]IP ubiq[tUbAb] is increased itpo xMdm2
    *** cells: LFS041<Tp53~null><xTp53><xUbiq> in BMS "treated with MG132"
    *** unaffected by: xMdm2(del(218-245))"del4" [substitution]
    *** source: 11340074-Fig-2b
  *** xTp53(R173H)[Ab]IP ubiq[tUbAb] is increased itpo xMdm2
    *** cells: LFS041<Tp53~null><xTp53><xUbiq> in BMS "treated with MG132"
    *** unaffected by: xMdm2(del(218-245))"del4" [substitution]
    *** source: 11340074-Fig-2b
  *** xTp53(R248W)[Ab]IP ubiq[tUbAb] is increased itpo xMdm2
    *** cells: LFS041<Tp53~null><xTp53><xUbiq> in BMS "treated with MG132"
    *** unaffected by: xMdm2(del(218-245))"del4" [substitution]
    *** source: 11340074-Fig-2b
  *** xTp53[Ab] ubiq[UbAb] is increased itpo xMdm2
    *** cells: U2OS<xTp53> in BMS "treated with ALLN"
    *** partially inhibited by: xTp53(K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** inhibited by: xTp53(mnr)"Mdm2-binding-mut" [substitution]
    *** source: 11713287-Fig-3b

requires Mdm2 Nterm

  *** xTp53[Ab]IP ubiq[tUbAb] is increased itpo xMdm2
    *** cells: LFS041<Tp53~null><xTp53><xUbiq> in BMS "treated with MG132"
    *** inhibited by: xMdm2(62-491)"delXM" [substitution]
    *** unaffected by: xMdm2(del(218-245))"del4" [substitution]
    *** source: 11340074-Fig-2a


  *** xTp53[Ab] ubiq[tUbAb-ppt] is increased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** enhanced by: MG132 [addition]
    *** enhanced by: xMdm4 [addition]
    *** source: 11606419-Fig-1a
  *** xTp53[Ab] ubiq[tUbAb-ppt] is increased itpo xMdm2
    *** cells: mEFs<Tp53~null><xTp53> in BMS "treated with MG132"
    *** source: 11606419-Fig-1b
  *** xTp53[Ab] ubiq[tUbAb-ppt] is increased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** enhanced by: MG132 [addition]
    *** enhanced by: xMdm4 [addition]
    *** source: 11606419-Fig-1c
  *** xTp53[Ab] ubiq[tUbAb-ppt] is increased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53><xSumo1> in BMS
    *** unaffected by: xSenp8 [addition]
    *** source: 15242646-Fig-2b
  *** xTp53[Ab] ubiq[tUbAb-ppt] is increased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53><xUbiq> in BMS
    *** source: 15242646-Fig-3g
  *** xTp53[Ab] ubiq[tUbAb-ppt] is unchanged itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53><xIsg15> in BMS
    *** source: 15242646-Fig-3g

  *** xTp53[Ab]IP ubiq[tUbAb] is increased itpo xMdm2
    *** cells: mEFs<Tp53~null><Mdm2~null><xTp53><xUbiq> in BMS
    *** source: 12654245-Fig-6c
  *** xTp53[Ab]IP ubiq[tUbAb] is increased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53><xUbiq> in BMS
    *** source: 12654245-Fig-6b
  *** xTp53[Ab]IP upshift[WBMS] is increased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53><xUbiq> in BMS
    *** source: 12654245-Fig-6b
  *** xTp53[Ab] upshift[WBMS] is increased itpo xMdm2
    *** cells: mEFs<Tp53~null><Mdm2~null><xTp53> in BMS "treated with MG132"
    *** source: 11046142-Fig-6a
  *** xTp53[Ab] ubiq[tUbAb-ppt] is increased itpo xMdm2
    *** cells: H1299<xTp53><xUbiq> in BMS
    *** source: 17098746-Fig-4a

======================================================================
xTp53 protein expression is decreased in the presence of Mdm2
======================================================================

required Mdm2 features

  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** reversed by: xMdm2(mnr)"del-Nterm" [substitution]
    *** source: 9153395-Fig-1a
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: HELA<xTp53> in BMS
    *** reversed by: xMdm2(mnr)"del-Nterm" [substitution]
    *** source: 9153395-Fig-1a
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: LFS041<Tp53~null><xTp53> in BMS
    *** reversed by: xMdm2(62-491)"delXM" [substitution]
    *** unaffected by: xMdm2(del(218-245))"del4" [substitution]
    *** source: 11340074-Fig-4a
  *** xTp53(R173H)[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: LFS041<Tp53~null><xTp53> in BMS
    *** reversed by: xMdm2(62-491)"delXM" [substitution]
    *** unaffected by: xMdm2(del(218-245))"del4" [substitution]
    *** source: 11340074-Fig-4a
  *** xTp53(R248W)[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: LFS041<Tp53~null><xTp53> in BMS
    *** reversed by: xMdm2(62-491)"delXM" [substitution]
    *** unaffected by: xMdm2(del(218-245))"del4" [substitution]
    *** source: 11340074-Fig-4a
  *** xTp53(V143A)[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: LFS041<Tp53~null><xTp53> in BMS
    *** reversed by: xMdm2(62-491)"delXM" [substitution]
    *** unaffected by: xMdm2(del(218-245))"del4" [substitution]
    *** source: 11340074-Fig-4a
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: U2OS<xTp53> in BMS
    *** reversed by: xMdm2(del(222-437)) [substitution]
    *** source: 9153396-Fig-6
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** reversed by: xMdm2(S395D) [substitution]
    *** source: 11331603-Fig-6a
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** reversed by: xMdm2(S166A) [substitution]
    *** reversed by: xMdm2(S186A) [substitution]
    *** reversed by: xMdm2(S166A/S186A) [substitution]
    *** source: 11504915-Fig-4c

required Tp53 features

  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** reversed by: xTp53(K120R/K164R/K370R/K372R/K373R/K381R/K382R/K386R)"8KR" [substitution]
    *** source: 18485870-Fig-2e
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** unaffected by: xTp53(K370R) [substitution]
    *** unaffected by: xTp53(K372R/K373R) [substitution]
    *** unaffected by: xTp53(K381R/K382R) [substitution]
    *** unaffected by: xTp53(K386R) [substitution]
    *** partially reversed by: xTp53(K370R/K372R/K373R) [substitution]
    *** partially reversed by: xTp53(K381R/K382R/K386R) [substitution]
    *** reversed by: xTp53(K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** source: 11046142-Fig-4a
  *** xTp53(Gal4)[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** unaffected by: xTp53((1-42)/Gal4) [substitution]
    *** source: 9153395-Fig-1b
  *** xTp53(del(13-53))[Ab] prot-exp[WB] is unchanged itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** source: 9153395-Fig-1b
  *** xTp53(del(13-19))[Ab] prot-exp[WB] is detectable
    *** cells: mEFs<Tp53~null><Mdm2~null><xTp53(del(13-19))> in BMS
    *** source: 9153396-Fig-3
  *** xTp53(del(13-19))[Ab] prot-exp[WB] is detectable
    *** cells: mEFs<Tp53~null><xTp53(del(13-19))> in BMS
    *** source: 9153396-Fig-3
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** reversed by: xTp53(del(13-19)) [substitution]
    *** source: 9153396-Fig-1b
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** reversed by: xTp53(del(13-19)) [substitution]
    *** source: 9153396-Fig-1c
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** reversed by: xTp53(del(13-19)) [substitution]
    *** source: 9153396-Fig-1d
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** unaffected by: xTp53(K381R/K382R/K386R) [substitution]
    *** reversed by: xTp53(K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** inhibited by: xTp53(mnr)"Mdm2-binding-mut" [substitution]
    *** source: 11713287-Fig-3c
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** reversed by: xTp53(del(22-23)) [substitution] 
    *** source: 9153396-Fig-1d
  *** xTp53(L14Q/F19S)[Ab] prot-exp[WB] is unchanged itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** source: 9153395-Fig-1b
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** unaffected by: xTp53(S15A) [substitution]
    *** unaffected by: xTp53(S15D) [substitution]
    *** source: 9153396-Fig-1d
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: mEFs<Tp53~null><Mdm2~null> in BMS
    *** unaffected by: xTp53(mnr)"NES-mut" [substitution]
    *** partially reversed by: xTp53(mnr)"NES-mut+vNES" [substitution]
    *** partially reversed by: xTp53(mnr)"WT+vNES" [substitution]
    *** source: 15029243-Fig-1e
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: U2OS<xTp53> in BMS
    *** unaffected by: xTp53(mnr)"NES" [substitution]
    *** reversed by: xTp53(mnr)"Mdm2-binding-mut" [substitution]
    *** source: 11713287-Fig-8b

Akt1--  ???

  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** enhanced by: xAkt1(mnr)"CA" [addition]
    *** reversed by: xAkt1(mnr)"KD" [addition]
    *** source: 11504915-Fig-4b

Crm1--

  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: U2OS<xTp53> in BMS
    *** partially reversed by: xCrm1 [addition]
    *** source: 11713287-Fig-8a

not Pirh2--

  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** does not req: Pirh2 [RNAi]
    *** source: 15103385-Fig-S1b

reversed in the presence of P14Arf

  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: U2OS<xTp53> in BMS
    *** reversed by: xP14Arf [addition]
    *** source: 9724636-Fig-6a
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: HELA<xTp53> in BMS
    *** reversed by: xP14Arf [addition]
    *** source: 9529248-Fig-4b
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: HELA<xTp53> in BMS
    *** reversed by: xP14Arf [addition]
    *** comment: 5X more xMdm2 and xP14Arf than xTp53 was used
    *** source: 9529249-Fig-5c

reversed in the presence of Mdm4

  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** reversed by: xMdm4 [addition]
    *** source: 11606419-Fig-1a
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: mEFs<Tp53~null><xTp53> in BMS
    *** reversed by: xMdm4 [addition]
    *** source: 11606419-Fig-1b
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** reversed by: xMdm4 [addition]
    *** source: 11606419-Fig-1c

requires at least equal concentrations of Mdm2 and Tp53

  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** comment: decrease requires at least equal concentrations of Tp53 and Mdm2
    *** source: 14671306-Fig-1d

iffy stuff about "mono" vs "poly" ubiquitination
note that "mono" ubiquitination shows multiple bands

  *** xTp53[Ab] ubiq[tUbAb-ppt] is increased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53><xUbiq> in BMS
    *** comment: only lower MW bands are seen when xUbiq(dig)"no-lysines" is substituted
    *** source: 14671306-Fig-3a
  *** xTp53[Ab] ubiq[tUbAb-ppt] is increased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53><xUbiq> in BMS "treated with ALLN and MG132"
    *** unaffected by: xUbiq(dig)"no-lysines" [substitution]
    *** comment: low ratios of Mdm2:Tp53 were used
    *** comment: there is slightly less Tp53-ubiq in the cytoplasmic fraction when xUbiq(dig)"no-lysine" is substituted
    *** source: 14671306-Fig-1e
  *** xTp53[Ab] ubiq[tUbAb-ppt] is increased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53><xUbiq> in BMS "treated with ALLN and MG132"
    *** unaffected by: xUbiq(dig)"no-lysines" [substitution]
    *** comment: low ratios of Mdm2:Tp53 were used
    *** source: 14671306-Fig-1c
  *** xTp53[tAb]IP ubiq[UbAb] is increased irt xMdm2
    *** cells: H1299<Tp53~null><xTp53><xUbiq> in BMS
    *** comment: only high MW bands are seen because a poly-Ubiq Ab was used
    *** comment: all the poly-Ubiq is in the nuclear fraction
    *** source: 14671306-Fig-S4b
  *** xTp53[tAb]IP ubiq[UbAb] is increased irt xMdm2
    *** cells: H1299<Tp53~null><xTp53><xUbiq> in BMS
    *** comment: only high MW bands are seen because a poly-Ubiq Ab was used
    *** comment: signal is less intense when when xUbiq(dig)"no-lysines" is substituted
    *** source: 14671306-Fig-S4a

requires proteasomal degradation - implies ubiq

  *** xTp53[Ab] prot-exp[WB] is unchanged itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53> in BMS "treated with MG132"
    *** source: 9153395-Fig-3c
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: U2OS<xTp53> in BMS
    *** reversed by: Lactacystin [addition]
    *** source: 9153396-Fig-5b
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** reversed by: MG132 [addition]
    *** source: 11046142-Fig-4b
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** inhibited by: MG132 [addition]
    *** source: 11606419-Fig-1a
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: mEFs<Tp53~null><xTp53> in BMS
    *** inhibited by: MG132 [addition]
    *** source: 11606419-Fig-1b
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** inhibited by: MG132 [addition]
    *** source: 11606419-Fig-1c

no extras

  *** xTp53[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: C33A in BMS
    *** source: 9153396-Fig-4b
  *** xTp53[Ab] prot-exp[WB] is detectable
    *** cells: mEFs<Tp53~null><Mdm2~null><xTp53> in BMS
    *** source: 9153396-Fig-3
  *** xTp53[Ab] prot-exp[WB] is undetectable
    *** cells: mEFs<Tp53~null><xTp53> in BMS
    *** source: 9153396-Fig-3
  *** xTp53(R273H)[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: mEFs<Tp53~null><Mdm2~null><xTp53(R273H)> in BMS
    *** source: 12963717-Fig-4a

==============================================
Increased protein expression in the absence of
==============================================

  *** Tp53[Ab] prot-exp[WB] is increased itao Mdm2 [RNAi]
    *** cells: BJ in BMS
    *** source: 15103385-Fig-4c
  *** Tp53[Ab] prot-exp[WB] is increased itao Mdm2 [RNAi]
    *** cells: U2OS in BMS
    *** source: 15103385-Fig-4c
  *** Tp53[Ab] prot-exp[WB] is increased itao Mdm2 [RNAi]
    *** cells: MCF7 in BMS
    *** source: 12963717-Fig-3a


===================================================================
decrease in Tp53 protein expression is due to Tp53 degradation not 
a decrease in Tp53 translation or transcription
===================================================================

  *** xTp53[Ab] prot-stability[35S-pulse-chase] is decreased itpo xMdm2
    *** cells: H1299 in BMS
    *** source: 9153395-Fig-3a
  *** xTp53[Ab] prot-stability[35S-pulse-chase] is decreased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** reversed by: xTp53(del(13-19)) [substitution]
    *** source: 9153396-Fig-2b
  *** xTp53[Ab] prot-stability[35S-pulse-chase] is decreased itpo xMdm2
    *** cells: HELA<xMdm2> in BMS
    *** reversed by: xP14Arf [addition]
    *** comment: 5X more xMdm2 and xP14Arf than xTp53 was used
    *** source: 9529249-Fig-5b

=================================
Effect on Tp53-gene transcription
=================================

  *** Tp53-gene mRNA[Northern] is increased itpo xMdm2
    *** cells: H1299 in BMS
    *** unaffected by: xMdm2(mnr)"del-Nterm" [substitution]
    *** source: 9153395-Fig-2
  *** Tp53-gene mRNA[Northern] is unchanged itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** source: 9153396-Fig-1d

=============================================
Increased protein stability in the absence of
=============================================

  *** Tp53[Ab]IP prot-stability[35S-pulse-chase] is increased itao Mdm2 [RNAi]
    *** cells: U2OS in BMS
    *** source: 15103385-Fig-4a
  *** Tp53[Ab] prot-exp[WB] is increased itao Mdm2 [RNAi]
    *** cells: U2OS in BMS
    *** source: 15989956-Fig-8a

=================
Downstream Events
=================

  *** Tp53-reporter[Luc] is decreased itpo xMdm2
    *** cells: MCF7 in BMS
    *** source: 12654245-Fig-7b
  *** Tp53-reporter[Luc] is decreased itpo xMdm2
    *** cells: mEFs<Tp53~null><Mdm2~null><xTp53> in BMS
    *** partially reversed by: xTp53(K386R) [substitution]
    *** partially reversed by: xTp53(K370R/K372R/K373R) [substitution]
    *** partially reversed by: xTp53(K381R/K382R/K386R) [substitution]
    *** reversed by: xTp53(K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** source: 11046142-Fig-8
  *** Tp53-reporter[Luc] is decreased itpo xMdm2
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** source: 12654245-Fig-7a
  *** Tp53-reporter[Luc] is decreased itpo xMdm2
    *** cells: LFS041<Tp53~null><xTp53> in BMS
    *** comment: not normalized for Tp53 protein
    *** reversed by: xMdm2(62-491)"delXM" [substitution]
    *** unaffected by: xMdm2(del(218-245))"del4" [substitution]
    *** source: 11340074-Fig-3a
  *** xTp53(1-71)[Gal4-DBD] Gal4-reporter[Luc] is decreased itpo xMdm2
    *** cells: LFS041<Tp53~null><xTp53(1-71)> in BMS
    *** comment: not normalized for Tp53 protein
    *** reversed by: xMdm2(62-491)"delXM" [substitution]
    *** unaffected by: xMdm2(del(218-245))"del4" [substitution]
    *** source: 11340074-Fig-3b
  *** xTp53(19-71)[Gal4-DBD] Gal4-reporter[Luc] is decreased itpo xMdm2
    *** cells: LFS041<Tp53~null><xTp53(1-71)> in BMS
    *** comment: not normalized for Tp53 protein
    *** reversed by: xMdm2(62-491)"delXM" [substitution]
    *** unaffected by: xMdm2(del(218-245))"del4" [substitution]
    *** source: 11340074-Fig-3b
  *** Tp53-reporter[Luc] is decreased itpo xMdm2
    *** cells: HEK293<xTp53> in BMS
    *** reversed by: xMdm2(S166A/S186A) [substitution]
    *** source: 11504915-Fig-4e
  *** Tp53-reporter[Luc] is decreased itpo xMdm2
    *** cells: HEK293<xTp53> in BMS
    *** unaffected by: xAkt1(mnr)"CA" [addition]
    *** reversed by: xAkt1(mnr)"KD" [addition]
    *** source: 11504915-Fig-4e
  *** Tp53-reporter[Luc] is decreased itpo xMdm2
    *** cells: MCF7<xTp53> in BMS
    *** reversed by: xMdm2(S166A/S186A) [substitution]
    *** source: 11504915-Fig-4e
  *** Tp53-reporter[Luc] is increased itpo xAkt1(mnr)"KD"
    *** cells: MCF7 in BMS
    *** source: 11504915-Fig-4e

