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Direct Ubiquitination
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Ube1 and Ube2d2 are supplied in rule 1405c.Cop1.ubiq.by.Ube2d2

  *** rTp53[Ab]IP ubiq[tUbAb] is increased by (rUbe1 + rUbe2d2 + rUbiq + rCop1)
    *** cells: none
    *** source: 15103385-Fig-2g

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Ubiquitination in the presence of
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  *** xTp53[Ab] ubiq[tUbAb-ppt] is increased itpo xCop1
    *** cells: SAOS2<Tp53~null><xTp53><xUbiq> in BMS
    *** reversed by: xCop1(mnr)"del-ring" [substitution]
    *** source: 15103385-Fig-2f

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Decreased protein expression in the presence of
===============================================

  *** Tp53[Ab] prot-exp[WB] is decreased itpo xCop1
    *** cells: mEFs<Mdm2~null> in BMS
    *** reversed by: xCop1(mnr)"del-ring" [substitution]
    *** source: 15103385-Fig-S1a
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xCop1
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** does not req: Pirh2 [RNAi]
    *** source: 15103385-Fig-S1b
  *** xTp53[Ab] prot-exp[WB] is decreased itpo xCop1
    *** cells: SAOS2<Tp53~null><xTp53><xCop1> in BMS
    *** inhibited by: xCop1(mnr)"del-ring" [substitution]
    *** source: 15103385-Fig-2a
  *** Tp53[Ab] prot-exp[WB] is decreased itpo xCop1
    *** cells: U2OS in BMS
    *** reversed by: ALLN [addition]
    *** source: 15103385-Fig-2e
  *** Tp53[Ab] prot-exp[WB] is decreased itpo xCop1
    *** cells: U2OS in BMS
    *** reversed by: xCop1(mnr)"del-ring" [substitution]
    *** source: 15103385-Fig-2b
  *** Tp53[Ab] prot-exp[WB] is decreased itpo xCop1
    *** cells: U2OS in BMS
    *** reversed by: xCop1(mnr)"del-ring" [substitution]
    *** source: 15103385-Fig-2e

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Increased protein expression in the absence of
==============================================

  *** Tp53[Ab] prot-exp[WB] is increased itao Cop1 [RNAi]
    *** cells: U2OS in BMS
    *** source: 15103385-Fig-3a
  *** Tp53[Ab] prot-exp[WB] is increased itao Cop1 [RNAi]
    *** cells: U2OS in BMS
    *** source: 15103385-Fig-4c
  *** Tp53[Ab] prot-exp[WB] is increased itao Cop1 [RNAi]
    *** cells: BJ in BMS
    *** source: 15103385-Fig-4c
  *** Tp53[Ab] prot-exp[WB] is increased itao Cop1 [RNAi]
    *** cells: U2OS in BMS
    *** source: 15989956-Fig-8a

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Decreased protein stability in the presence of
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  *** Tp53[Ab]IP prot-stability[35S-pulse-chase] is decreased itpo xCop1
    *** cells: HEK293T in BMS
    *** source: 15103385-Fig-2d

=============================================
Increased protein stability in the absence of
=============================================

  *** Tp53[Ab]IP prot-stability[35S-pulse-chase] is increased itao Cop1 [RNAi]
    *** cells: U2OS in BMS
    *** source: 15103385-Fig-4a

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No effect on Tp53-gene transcription
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  *** Tp53-gene mRNA[RTPCR] is unchanged itpo xCop1
    *** cells: U2OS in BMS
    *** unaffected by: xCop1(mnr)"del-ring" [substitution]
    *** source: 15103385-Fig-2c
