=============
Oligo binding
=============

requires K373R, Ep300

  *** rTp53[Ab] oligo-binding[EMSA] is detectable
    *** cells: none
    *** comment: enhanced by pre-acetyl of rTp53 by rEp300(1195-1810)
    *** source: 9288740-Fig-5b
  *** rTp53 oligo-binding[EMSA] is detectable
    *** cells: none
    *** comment: enhanced by pre-acetyl with rEp300
    *** source: 9744860-Fig-5a

==============
Tp53 reporters
==============

Ep300--

  *** Tp53-reporter[Luc] is increased itpo xEp300
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** inhibited by: xTp53(S33A/S46A/T81A/S127A/T150A/S315A) [substitution]
    *** source: 17906639-Fig-S4a

Tp53 phos sites

  *** Tp53-reporter[Luc] is increased itpo xTp53
    *** cells: SAOS2<Tp53~null> in BMS
    *** unaffected by: xTp53(K370R) [substitution]
    *** unaffected by: xTp53(K372R/K373R) [substitution]
    *** unaffected by: xTp53(K381R/K382R) [substitution]
    *** enhanced by: xTp53(K386R) [substitution]
    *** enhanced by: xTp53(K370R/K372R/K373R) [substitution]
    *** enhanced by: xTp53(K381R/K382R/K386R) [substitution]
    *** enhanced by: xTp53(K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** source: 11046142-Fig-2

Tp53 acetyl sites

  *** Tp53-reporter[Luc] is increased itpo xTp53
    *** cells: HELA in BMS
    *** enhanced by: xTp53(K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** source: 11046142-Fig-7b

enhanced by Pin1 which requires phos(S33/T81/S315) for binding to Tp53

  *** Tp53-reporter[Luc] is increased itpo xPin1
    *** cells: U2OS<xTp53> in BMS
    *** inhibited by: xPin1(1-38)"WW-domain" [substitution]
    *** source: 12397362-Fig-S2a
  *** Tp53-reporter[Luc] is increased itpo xPin1
    *** cells: U2OS<xTp53> in BMS
    *** inhibited by: xTp53(T81A/S315A) [substitution]
    *** inhibited by: xTp53(S33A/S315A) [substitution]
    *** inhibited by: xTp53(S33A/T81A/S315A) [substitution]
    *** source: 12397362-Fig-S2b

requires Tp53 phos(S33/S46/T81/S127/T150/S315)

  *** Tp53-reporter[Luc] is increased itpo xTp53
    *** cells: SAOS2<Tp53~null> in BMS
    *** inhibited by: xTp53(S33A/S46A/T81A/S127A/T150A/S315A) [substitution]
    *** source: 17906639-Fig-S4b












