=======================
direct phosphorylation
=======================

  *** rStat1(695-710)[32P] phos[32P-ATP] is increased by xJak2[Ab]IP
    *** cells: none
    *** IPfrom: SF9 in BMS
    *** inhibited by: rStat1(14-29) [substitution]
    *** source: 7657660-Fig-4
  *** pStat1[32P] phos[32P-ATP] is increased by xJak2[Ab]IP
    *** cells: none
    *** IPfrom: SF9 in BMS
    *** comment: Stat1 from kinase reaction binds to a GAS oligo
    *** inhibited by: xJak2(L882E)[Ab]IP [substitution]
    *** source: 7657660-Fig-5b,6a
  *** pStat1[pYAb] Yphos[pYAb] is increased by xJak2[Ab]IP
    *** cells: none
    *** IPfrom: SF9 in BMS
    *** inhibited by: xJak2(L882E)[Ab]IP [substitution]
    *** source: 7657660-Fig-6b


============
DNA binding
============

  *** xStat1[Ab] oligo-binding[EMSA] is increased itpo xJak2
    *** cells: COS7 in BMS
    *** source: 9373245-Fig-1b
  *** Stat1[Ab] oligo-binding[EMSA] is increased itpo xJak2
    *** cells: COS7 in BMS
    *** source: 9373245-Fig-5
  *** xStat1[Ab] oligo-binding[EMSA] is increased itpo xJak2
    *** cells: SF9 in BMS
    *** oligo: GAS 5-CTAGCAGTGTTTCCCCGAAACACGCTAG-3
    *** inhibited by: xJak2(L882E) [substitution]
    *** source: 7657660-Fig-3a

=====
Yphos
=====

  *** xStat1[Ab]IP Yphos[pYAb] is increased itpo xJak2
    *** cells: COS7 in BMS
    *** source: 9373245-Fig-1a
  *** xStat1[Ab]IP Yphos[pYAb] is increased itpo xJak2
    *** cells: SF9 in BMS
    *** source: 9373245-Fig-8a
  *** xStat1[Ab]IP Yphos[pYAb] is increased itpo xJak2
    *** cells: SF9 in BMS
    *** inhibited by: xJak2(L882E) [substitution]
    *** source: 7657660-Fig-1a

==============
colocalization
==============

  *** xStat1[Ab] colocwith[IHC] Jak2[Ab] is detectable
    *** cells: U3A<Stat1~null> in BMS
    *** unaffected by: xStat1(K410Q/K413Q) [substitution]
    *** comment: colocalization is in the cytoplasm
    *** source: 19171783-Fig-1j
