====================================
Direct acetyl of Smad7 by Ep300
====================================

  *** rSmad7 acetyl[KAcAb] is increased by rEp300(1135-1810)"HAT-domain"
    *** cells: none
    *** comment: Rabbit Reticulocyte Lysate is added as a source of ubiquitination machinery
    *** inhibited by: rEp300(1135-1810)"HAT-domain" [addition]
    *** source: 12408818-Fig-5f

=============================
acetyl on Smad7(K64/K70)
=============================

  --- direct ---
  *** rSmad7[14C] acetyl[14C-acetyl-CoA] is increased by rEp300(1135-1810)"HAT-domain"
    *** cells: none
    *** unaffected by: rSmad7(2-261)"Nterm" [substitution]
    *** inhibited by: rSmad7(204-402)"Cterm" [substitution]
    *** comment: K64 and K70 were shown to be acetylated by MALDI-TOF-MS
    *** source: 12408818-Fig-3a,3b
  *** rSmad7[14C] acetyl[14C-acetyl-CoA] is increased by rEp300(1135-1810)"HAT-domain"
    *** cells: none
    *** partially inhibited by: rSmad7(K64A) [substitution]
    *** unaffected by: rSmad7(K70A) [substitution]
    *** inhibited by: rSmad7(K64A/K70A) [substitution]
    *** source: 12408818-Fig-4a
  --- itpo ---
  *** xSmad7[tAb]IP acetyl(K64)[AcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** enhanced by: TrichostatinA [addition]
    *** inhibited by: xSmad7(K64A) [substitution]
    *** source: 15831498-Fig-1b
  *** xSmad7[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** partially inhibited by: xSmad7(K64A) [substitution]
    *** unaffected by: xSmad7(K70A) [substitution]
    *** inhibited by: xSmad7(K64A/K70A) [substitution]
    *** source: 12408818-Fig-4b


===================================================
Total acetyl of Smad7 is increased itpo Ep300
===================================================

  *** xSmad7[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** inhibited by: xHdac1 [addition]
    *** unaffected by: xHdac2 [addition]
    *** inhibited by: xHdac3 [addition]
    *** partially inhibited by: xHdac4 [addition]
    *** partially inhibited by: xHdac5 [addition]
    *** inhibited by: xHdac6 [addition]
    *** source: 15831498-Fig-4a
  *** xSmad7[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** repressed by: Hdac1 [RNAi]
    *** source: 15831498-Fig-4b
  *** xSmad7[tAb]IP acetyl[3H-acetate] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** source: 12408818-Fig-2a
  *** xSmad7[tAb]IP acetyl[KAcAb] is decreased itpo xEp300(mnr)"HAT-deficient"
    *** cells: HELA in BMS
    *** source: 12408818-Fig-7a
  *** xSmad7[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** inhibited by: xEp300(mnr)"HAT-deficient" [substitution]
    *** source: 12408818-Fig-4d
  *** xSmad7[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** partially inhibited by: xTgfbR1(mnr)"CA" [addition]
    *** source: 12408818-Fig-2a
  *** xSmad7[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** source: 17478422-Fig-1a
  --- acetyl is increased when deacetylases are inhibited with TrichostatinA ---
  *** xSmad7[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** enhanced by: TrichostatinA [addition]
    *** source: 15831498-Fig-1a
  *** xSmad7[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** enhanced by: TrichostatinA [addition]
    *** source: 12408818-Fig-4b

============================================
Physical interaction between Smad7 and Ep300
============================================

  *** rEp300(2-411)[35S] boundby[WB] rSmad7[GST] is detectable
    *** cells: none
    *** inhibited by: rEp300(412-1150) [substitution]
    *** unaffected by: rEp300(1151-1673) [substitution]
    *** unaffected by: rEp300(1674-2414) [substitution]
    *** source: 12408818-Fig-1b
  *** rEp300(1135-1810)"HAT-domain"[35S] boundby[WB] rSmad7[GST] is detectable
    *** cells: none
    *** unaffected by: rSmad7(2-261)"Nterm" [substitution]
    *** inhibited by: rSmad7(204-402)"Cterm" [substitution]
    *** source: 12408818-Fig-1c
  *** xEp300[Ab] snaggedby[WB] rSmad7[GST] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xEp300(mnr)"HAT-deficient" [substitution]
    *** source: 12408818-Fig-4e
  *** xSmad7[tAb] copptby[WB] xEp300[tAb] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xSmad7(2-261)"Nterm" [substitution]
    *** unaffected by: xSmad7(204-402)"Cterm" [substitution]
    *** source: 12408818-Fig-1a
  --- association does not require Smad7 acetyl sites ---
  *** xSmad7[tAb] copptby[WB] xEp300[tAb] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xSmad7(K64A/K70A) [substitution]
    *** source: 12408818-Fig-4c

====================================
Smad7 acetyl in untreated cells
====================================

  *** Smad7[Ab]IP acetyl[KAcAb] is detectable
    *** cells: HACAT in BMS
    *** source: 12408818-Fig-4f
  --- basal acetyl requires Ep300 ---
  *** xSmad7[tAb]IP acetyl[KAcAb] is detectable
    *** cells: HACAT in BMS
    *** reqs: Ep300 [RNAi]
    *** source: 12408818-Fig-2b
