========================================================
Smad4 return to cytoplasm requires Crm1 (chemicals only)
========================================================

  *** xSmad4[tAb] locatedin(cytoplasm)[IHC] is decreased irt LMB (30 min)
    *** cells: C2C12 in BMS
    *** comment: xSmad4 was injected into the nucleus and incubated for 30 min
    *** source: 11265759-Fig-2b
  *** Smad4[Ab] locatedin(cytoplasm)[IHC] is increased irt SB431542 (120 min)
    *** cells: HACAT in BMS "treated with CHX" "treated with Tgfb1 for 60 min"
    *** inhibited by: LMB [addition]
    *** source: 12191474-Fig-5c
  *** Smad4[Ab] locatedin(cytoplasm)[IHC] is increased irt LMB (times)
    *** cells: HACAT in BMS
    *** times: 0 10+ 60+ min
    *** source: 11074002-Fig-6a
  *** xSmad4[tAb] locatedin(nucleus)[IHC] is increased irt LMB (4 hr)
    *** cells: HELA in BMS
    *** source: 12191473-Fig-3c

========================================================
Smad4 return to cytoplasm requires the Smad4-NES
========================================================

  *** xSmad4[tAb] locatedin(cytoplasm)[IHC] is detectable
    *** cells: C2C12 in BMS
    *** comment: xSmad4 was injected into the nucleus and incubated for 30 min
    *** inhibited by: xSmad4(L146A/L148A)"NES-mutant" [substitution]
    *** source: 11265759-Fig-2b
  *** xSmad4[tAb] locatedin(nucleus)[IHC] is detectable
    *** cells: NIH3T3 in BMS
    *** comment: much of the xSmad4 is in the cytoplasm
    *** unaffected by: xSmad4(K45A/K46A/K48A/K49A)"NLS-mut" [substitution]
    *** enhanced by: xSmad4(L146A/L148A)"NES-mut" [substitution]
    *** source: 11074002-Fig-5b
  *** xSmad4[tAb] locatedin(nucleus)[IHC] is detectable
    *** cells: NIH3T3 in BMS
    *** comment: most of the xSmad4 is in the cytoplasm
    *** enhanced by: xSmad4(del(142-153)) [substitution]
    *** enhanced by: xSmad4(del(153-223)) [substitution]
    *** unaffected by: xSmad4(del(263-302)) [substitution]
    *** unaffected by: xSmad4(del(223-302)) [substitution]
    *** enhanced by: xSmad4(del(153-302)) [substitution]
    *** enhanced by: xSmad4(del(153-320)) [substitution]
    *** comment: treatment with LMB causes retention of xSmad4 in nucleus regardless of starting state
    *** source: 11074002-Fig-4

========================================================
Smad4 with a mutated NES accumulates in the nucleus
========================================================

  *** xSmad4(L146A/L148A)"NES-mutant"[tAb] locatedin(nucleus)[IHC] is detectable
    *** cells: C2C12 in BMS
    *** unaffected by: LMB [addition]
    *** source: 11265759-Fig-2a
  *** xSmad4(L146A/L148A)"NES-mutant"[tAb] locatedin(nucleus)[IHC] is detectable
    *** cells: C2C12 in BMS
    *** unaffected by: Tgfb1 (1 hr) [stim]
    *** inhibited by: LMB (30 min) [stim]
    *** comment: addition of the NES from Mek1(32-43) to xSmad4(L146A/L148A)"NES-mutant" N-term inhibits nuclear accumulation
    *** source: 11265759-Fig-5a

===========================================================
Smad4 with an extra N-term NES accumulates in the cytoplasm
===========================================================

  *** xSmad4[tAb] locatedin(cytoplasm)[IHC] is detectable
    *** cells: C2C12 in BMS
    *** comment: addition of the NES from Mek1(32-43) to xSmad4 N-term enhanced cytoplasmic accumulation
    *** source: 11265759-Fig-5a

====================================================
xSmad4 accumulates in the cytoplasm without stimulus
=====================================================

  *** xSmad4[tAb] locatedin(cytoplasm)[IHC] is detectable
    *** cells: C2C12 in BMS
    *** comment: treatment with LMB changes location to both cyto and nuc
    *** source: 11265759-Fig-2a

