===================================================
direct sumoylation by Sae1(E1a) Uba2(E1b) Ube2i(E2)
===================================================

  *** rSmad4[35S] upshift[WBMS] is increased by (rSumo1(1-97) + rUbe2i + rSae1 + rUba2)
    *** cells: none
    *** inhibited by: rSmad4(K113R/K159R) [substitution]
    *** partially inhibited by: rSmad4(K159R) [substitution]
    *** partially inhibited by: rSmad4(K113R) [substitution]
    *** unaffected by: rSmad4(K122R) [substitution]
    *** unaffected by: rSmad4(K106R) [substitution]
    *** unaffected by: rSmad4(K392R) [substitution]
    *** source: 14750902-Fig-1b

===========================================
Ube2i as a Smad4 Sumo-conjugating enzyme E2
===========================================

  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xSumo1
    *** cells: HELA in BMS
    *** enhanced by: xUbe2i [addition]
    *** source: 12740389-Fig-2d
  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xSumo1
    *** cells: HELA in BMS
    *** enhanced by: xUbe2i [addition]
    *** source: 12740389-Fig-3a
  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xUbe2i
    *** cells: HELA<xSumo1> in BMS
    *** source: 12740389-Fig-3b

===============================
Pias2 as a Smad4 Sumo-ligase E3
===============================

  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xUbe2i
    *** cells: HELA<xSumo1> in BMS
    *** enhanced by: xPias2 [addition]
    *** source: 12740389-Fig-3a
  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xPias2
    *** cells: HELA<xSumo1> in BMS
    *** inhibited by: xSmad4(K113R/K159R) [substitution]
    *** source: 12740389-Fig-3a
  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xPias2
    *** cells: HELA<xSumo1><xUbe2i> in BMS
    *** partially inhibited by: xSmad4(K113R/K159R) [substitution]
    *** comment: substitution of xSmad4(K113R/K159R) causes a decrease in the mono-di-tri sumoylated forms but not the poly forms
    *** source: 12740389-Fig-3a

===============================
Pias4 as a Smad4 Sumo-ligase E3
===============================

  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xUbe2i
    *** cells: HELA<xSumo1> in BMS
    *** enhanced by: xPias4 [addition]
    *** source: 12740389-Fig-3a
  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xPias4
    *** cells: HELA<xSumo1> in BMS
    *** inhibited by: xSmad4(K113R/K159R) [substitution]
    *** source: 12740389-Fig-3a
  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xPias4
    *** cells: HELA<xSumo1><xUbe2i> in BMS
    *** inhibited by: xSmad4(K113R/K159R) [substitution]
    *** source: 12740389-Fig-3a

====================================
Smad4 is sumoylated on K113 and K159
====================================

  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xSumo1
    *** cells: HELA in BMS
    *** partially inhibited by: xSmad4(K113R) [substitution]
    *** partially inhibited by: xSmad4(K159R) [substitution]
    *** inhibited by: xSmad4(K113R/K159R) [substitution]
    *** source: 12740389-Fig-2d
  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xUbe2i
    *** cells: HELA<xSumo1> in BMS
    *** partially inhibited by: xSmad4(K113R) [substitution]
    *** partially inhibited by: xSmad4(K159R) [substitution]
    *** inhibited by: xSmad4(K113R/K159R) [substitution]
    *** source: 12740389-Fig-2d
  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xSumo1
    *** cells: HELA in BMS
    *** inhibited by: xSmad4(K113R/K159R) [substitution]
    *** source: 12740389-Fig-3a
  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xUbe2i
    *** cells: HELA<xSumo1> in BMS
    *** inhibited by: xSmad4(K113R/K159R) [substitution]
    *** source: 12740389-Fig-3a
  *** xSmad4[tAb] upshift[WBMS] is increased itpo xSumo1
    *** cells: COS in BMS
    *** partially inhibited by: xSmad4(K159R) [substitution]
    *** partially inhibited by: xSmad4(K113R) [substitution]
    *** inhibited by: xSmad4(K113R/K159R) [substitution]
    *** source: 14750902-Fig-1c
  *** xSmad4[tAb] sumo[tSuAb] is increased itpo xSumo1
    *** cells: COS in BMS
    *** inhibited by: xSmad4(K113R/K159R) [substitution]
    *** partially inhibited by: xSmad4(K159R) [substitution]
    *** partially inhibited by: xSmad4(K113R) [substitution]
    *** source: 14750902-Fig-1c
  *** xSmad4[tAb]IP upshift[WBMS] is detectable
    *** cells: HELA<xSumo1> in BMS
    *** partially inhibited by: xSmad4(K159R) [substitution]
    *** partially inhibited by: xSmad4(K113R) [substitution]
    *** inhibited by: xSmad4(K159R/K113R) [substitution]
    *** source: 12621041-Fig-2d
  *** xSmad4[tAb]IP sumo[tSuAb] is detectable
    *** cells: HELA<xSumo1> in BMS
    *** partially inhibited by: xSmad4(K159R) [substitution]
    *** partially inhibited by: xSmad4(K113R) [substitution]
    *** inhibited by: xSmad4(K159R/K113R) [substitution]
    *** source: 12621041-Fig-2d
  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xSumo1
    *** cells: HELA in BMS
    *** inhibited by: xSmad4(K159R/K113R) [substitution]
    *** source: 12621041-Fig-5c
  *** xSmad4[tAb]IP upshift[WBMS] is detectable
    *** cells: HELA<xSumo1> in BMS
    *** partially inhibited by: xSmad4(K159R) [substitution]
    *** unaffected by: xSmad4(K122R) [substitution]
    *** source: 12621041-Fig-2c
  *** xSmad4[tAb]IP sumo[tSuAb] is detectable
    *** cells: HELA<xSumo1> in BMS
    *** partially inhibited by: xSmad4(K159R) [substitution]
    *** unaffected by: xSmad4(K122R) [substitution]
    *** source: 12621041-Fig-2c
  -------------------------------------------------------------
  *** xSmad4(1-300)[tAb]IP sumo[tSuAb] is increased itpo xSumo1
    *** cells: HELA in BMS
    *** unaffected by: xSmad4(1-300/K51R) [substitution]
    *** unaffected by: xSmad4(1-300/K106R) [substitution]
    *** partially inhibited by: xSmad4(1-300/K159R) [substitution]
    *** partially inhibited by: xSmad4(1-300/K51R/K10R/K159R) [substitution]
    *** source: 12740389-Fig-2b
  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xSumo1
    *** cells: HELA in BMS
    *** partially inhibited by: xSmad4(1-140)"N-term" [substitution]
    *** unaffected by: xSmad4(1-300)"LN-term" [substitution]
    *** inhibited by: xSmad4(266-552)"C-term" [substitution]
    *** source: 12740389-Fig-2a
  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xSumo1
    *** cells: HELA in BMS
    *** partially inhibited by: xSmad4(K159R) [substitution]
    *** partially inhibited by: xSmad4(K51R/K159R) [substitution]
    *** partially inhibited by: xSmad4(K122R/K159R) [substitution]
    *** unaffected by: xSmad4(K51R) [substitution]
    *** unaffected by: xSmad4(K106R) [substitution]
    *** unaffected by: xSmad4(K122R) [substitution]
    *** unaffected by: xSmad4(K392R) [substitution]
    *** source: 12740389-Fig-2c
  *** xSmad4(2-552)[tAb]IP upshift[WBMS] is detectable
    *** cells: HELA in BMS
    *** unaffected by: xSmad4(2-185) [substitution]
    *** inhibited by: xSmad4(184-552) [substitution]
    *** source: 12621041-Fig-2b

=======================================
Smad4-sumo can use Sumo1 Sumo2 or Sumo3
=======================================

  *** xSmad4[tAb] upshift[WBMS] is increased itpo xSumo1
    *** cells: COS in BMS
    *** source: 14750902-Fig-1d
  *** xSmad4[tAb] upshift[WBMS] is increased itpo xSumo2
    *** cells: COS in BMS
    *** inhibited by: xSmad4(K113R/K159R) [substitution]
    *** source: 14750902-Fig-1d
  *** xSmad4[tAb] upshift[WBMS] is increased itpo xSumo3
    *** cells: COS in BMS
    *** source: 14750902-Fig-1d

===================
Smad4 is sumoylated
===================

  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xSumo1
    *** cells: HELA in BMS
    *** source: 12740389-Fig-1a
  *** xSmad4[tAb]IP sumo[tSuAb] is increased itpo xSumo1
    *** cells: HELA in BMS
    *** source: 12740389-Fig-1b

  *** Smad4[Ab]IP sumo[SuAb] is detectable
    *** cells: COS in BMS
    *** source: 14750902-Fig-1e
  *** Smad4[Ab]IP sumo[SuAb] is detectable
    *** cells: HELA in BMS
    *** source: 12621041-Fig-3
  *** Smad4[Ab]IP upshift[WBMS] is detectable
    *** cells: CHO in BMS
    *** source: 12621041-Fig-3
  *** Smad4[Ab]IP upshift[WBMS] is detectable
    *** cells: HELA in BMS
    *** source: 12621041-Fig-3
  *** Smad4[Ab]IP upshift[WBMS] is detectable
    *** cells: HM3 in BMS
    *** source: 12621041-Fig-3


  *** xSmad4[tAb]IP sumo[SuAb] is detectable
    *** cells: HELA in BMS
    *** source: 12621041-Fig-2a
  *** xSmad4[tAb]IP upshift[WBMS] is detectable
    *** cells: HELA in BMS
    *** source: 12621041-Fig-2a
