==================================================
Smad2 activation is defined as ability to bind DNA
==================================================
Smad2 DNA binding requires acetyl on K19
==================================================

  *** rSmad2((9-79)/(108-176))"MH2-domain" oligo-binding[EMSA] is detectable
    *** cells: none
    *** unaffected by: rSmad2((9-79)/(108-176)/K19R) [substitution]
    *** unaffected by: rSmad2((9-79)/(108-176)/K19Q) [substitution]
    *** source: 17074756-Fig-5f
  *** rSmad2(del(79-108)/K19Q) oligo-binding[EMSA] is detectable
    *** cells: none
    *** inhibited by: rSmad2(del(79-108)/K19R) [substitution]
    *** inhibited by: rSmad2(del(79-108)) [substitution]
    *** source: 17074756-Fig-5e

===================================================
increase in activity itpo Ep300 requires Smad2(K12)
===================================================

  *** CAGA-reporter[Luc] is increased itpo xEp300
    *** cells: HEPG2<xSmad2(del(79-108))> in BMLS "treated with Tgfb1 for 12 hr"
    *** inhibited by: xSmad2(del(79-108)/K19R) [substitution]
    *** source: 17074756-Fig-4a
  *** CAGA-reporter[Luc] is increased itpo xEp300
    *** cells: HEPG2<xSmad2(del(79-108))> in BMLS "treated with Tgfb1 for 12 hr"
    *** inhibited by: xSmad2(del(79-108)/K19R) [substitution]
    *** source: 17074756-Fig-4c
  *** xSmad2(del(79-108))[tAb] oligo-binding[EMSA] is increased itpo (xEp300 +  xTgfbR1(mnr)"CA")
    *** cells: COS1 in BMS
    *** inhibited by: xSmad2(del(79-108)/K19R) [substitution]
    *** comment: bound xSmad2 is acetylated on K19 [AcAb supershift]
    *** source: 17074756-Fig-5a

===================================================
increase in activity itpo Pcaf requires Smad2(K12)
===================================================

  *** rSmad2(del(79-108)){acetyl} oligo-binding[EMSA] is detectable
    *** cells: none
    *** inhibited by: rSmad2(del(79-108)/K19R) [substitution]
    *** comment: rSmad2(del(79-108)) was acetylated by Pcaf
    *** source: 17074756-Fig-5c
  *** CAGA-reporter[Luc] is increased itpo xPcaf
    *** cells: HEPG2<xSmad2(del(79-108))> in BMLS "treated with Tgfb1 for 12 hr"
    *** inhibited by: xSmad2(del(79-108)/K19R) [substitution]
    *** source: 17074756-Fig-4b
  *** xSmad2(del(79-108))[tAb] oligo-binding[EMSA] is increased itpo (xPcaf +  xTgfbR1(mnr)"CA")
    *** cells: COS1 in BMS
    *** inhibited by: xSmad2(del(79-108)/K19R) [substitution]
    *** source: 17074756-Fig-5b

======================================================
inhibition of activity by acetyltransferase inhibitors
======================================================

  *** xSmad2(del(79-108))[tAb] boundto(Pai1-gene)[ChIP] is detectable
    *** cells: COS1<xTgfbR1(mnr)"CA"> in BMS
    *** inhibited by: AnacardicAcid [addition]
    *** source: 17074756-Fig-6c

=====================================
Smad2(K19Q) is an acetyl mimetic
=====================================

  *** xSmad2(del(79-108)/K19Q)[tAb] boundto(Pai1-gene)[ChIP] is detectable
    *** cells: COS1<xTgfbR1(mnr)"CA"> in BMS
    *** unaffected by: AnacardicAcid [addition]
    *** source: 17074756-Fig-6c















