==============================
Smad7 ubiquitination by Smurf1
==============================

  *** xSmad7[tAb]IP ubiq[tUbAb] is increased itpo xSmurf1
    *** cells: COS7<xUbiq> in BMS
    *** inhibited by: xSmurf1(C710A)"CIA" [substitution]
    *** inhibited by: xSmad7(del(207-211))"PY-domain-deleted" [substitution]
    *** source: 11278251-Fig-3b
  *** xSmad7[tAb]IP ubiq[tUbAb] is increased itpo xSmurf1
    *** cells: COS7<xUbiq> in BMS
    *** source: 11278251-Fig-3a
  *** xSmad7[tAb]IP ubiq[tUbAb] is increased itpo xSmurf1
    *** cells: HEK293T<xUbiq> in BMS
    *** inhibited by: xSmad7(K64A/K70A) [substitution]
    *** source: 12408818-Fig-8c
  *** xSmad7[tAb]IP ubiq[tUbAb] is increased itpo xSmurf1
    *** cells: HEK293T in BMS "treated with Lactacystin"
    *** enhanced by: xSmurf1(del(22-37))"del-C2-domain" [addition]
    *** source: 12151385-Fig-3a

==================
Protein Expression
==================

  *** xSmad7[tAb]IP prot-exp[WB] is decreased itpo xSmurf1
    *** cells: COS7 in BMS
    *** unaffected by: xSmurf1(del(22-37))"del-C2-domain" [substitution]
    *** source: 12151385-Fig-4a

=================
Protein Stability
=================

  *** xSmad7[tAb] prot-stability[35S-pulse-chase] is decreased itpo xSmurf1
    *** cells: COS7 in BMS
    *** inhibited by: xSmurf1(C710A)"CIA" [substitution]
    *** source: 11278251-Fig-4a,4b

=============================================
Physical interaction between Smad7 and Smurf1
=============================================

  *** xSmad7[tAb] copptby[WB] xSmurf1(C710A)"CIA"[tAb] is detectable
    *** cells: COS7 in BMS
    *** source: 11278251-Fig-1b
  *** xSmad7[tAb] copptby[WB] xSmurf1(C710A)"CIA"[tAb] is detectable
    *** cells: COS7 in BMS
    *** unaffected by: xTgfbR1(T407D)"CA" [addition]
    *** enhanced by: Lactacystin [addition]
    *** source: 11278251-Fig-1c
  *** xSmad7[tAb] copptby[WB] xSmurf1[tAb] is detectable
    *** cells: COS7 in BMS "treated with Lactacystin"
    *** inhibited by: xSmad7(del(207-211))"PY-domain-deleted" [substitution]
    *** source: 11278251-Fig-1c
  *** xSmad7[tAb] copptby[WB] xSmurf1[tAb] is detectable
    *** cells: COS7 in BMS
    *** unaffected by: xSmurf1(I638A/L640A)"NES-mut" [substitution]
    *** source: 12519765-Fig-3b
  ------------------------------------------------------------------
  *** xSmurf1[tAb] copptby[WB] xSmad7[tAb] is detectable
    *** cells: COS7 in BMS "treated with Lactacystin"
    *** inhibited by: xSmad7(del(207-211))"PY-domain-deleted" [substitution]
    *** unaffected by: xTgfbR1(T407D)"CA" [addition]
    *** source: 11278251-Fig-1c
  *** xSmurf1(C699A)[tAb] copptby[WB] xSmad7[tAb] is detectable
    *** cells: COS7 in BMS
    *** unaffected by: xSmurf1(del(22-37)/C699A) [substitution]
    *** source: 12151385-Fig-2a

