======================
Direct Phosphorylation
======================

  *** rSgk1(61-431)[phosAb] phos(T256)[phosAb] is increased by rPdpk1
    *** cells: none
    *** unaffected by: Rictor[Ab]IP [addition]
    *** IPfrom: HEK293 in BMS
    *** source: 18925875-Fig-3c

===========================
Semi-Direct Phosphorylation
===========================

phos(T256)

  *** xSgk1[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: xSgk1 IP was pretreated with PP2A to remove prior phos
    *** unaffected by: xSgk1(K127A) [substitution]
    *** inhibited by: xSgk1(T256A) [substitution]
    *** unaffected by: xSgk1(S422A) [substitution]
    *** inhibited by: xSgk1(T256A/S422A) [substitution]
    *** source: 10191262-Fig-5a

Total Phos

  *** xSgk1[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: xSgk1 IP was pretreated with PP2A to remove prior phos
    *** enhanced by: xSgk1(S422D) [substitution]
    *** source: 10191262-Fig-5c
  *** xSgk1[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: xSgk1 IP was pretreated with PP2A to remove prior phos
    *** unaffected by: PIP3 [addition]
    *** unaffected by: PS + PC [addition]
    *** source: 10191262-Fig-4a
  *** xSgk1(61-431)[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1(61-431)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: xSgk1 IP was pretreated with PP2A to remove prior phos
    *** inhibited by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2b
  *** xSgk1(61-431)[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1(61-431)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: xSgk1 IP was pretreated with PP2A to remove prior phos
    *** inhibited by: PIFtide [addition]
    *** source: 11500365-Fig-3b

============================================================
Basal phosphorylation on T256 requires Raptor but not Rictor
============================================================

  *** Sgk1[phosAb] phos(T256)[phosAb] is detectable
    *** cells: WM35<xMtor> in BMS
    *** does not req: Rictor [RNAi]
    *** source: 18570873-Fig-7a
  *** Sgk1[phosAb] phos(T256)[phosAb] is detectable
    *** cells: WM35<xMtor> in BMS
    *** reqs: Raptor [RNAi]
    *** source: 18570873-Fig-7a

========================================================
Sgk1 is not phosed on T256 by Pdpk1 until S422 is phosed
========================================================

  *** xSgk1(61-431)[GST]IP phos(T256)[phosAb] is unchanged by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1(61-431)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: xSgk1 IP was pretreated with PP2A to remove prior phos
    *** unaffected by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2b
  *** xSgk1(61-431)[GST]IP phos(T256)[phosAb] is unchanged by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1(61-431)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: xSgk1 IP was pretreated with PP2A to remove prior phos
    *** unaffected by: PIFtide [addition]
    *** source: 11500365-Fig-3b

  *** xSgk1(61-431/S422D)[GST]IP phos(T256)[phosAb] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1(61-431/S422D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: xSgk1 IP was pretreated with PP2A to remove prior phos
    *** inhibited by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2b
  *** xSgk1(61-431/S422D)[GST]IP phos(T256)[phosAb] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1(61-431/S422D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: xSgk1 IP was pretreated with PP2A to remove prior phos
    *** inhibited by: PIFtide [addition]
    *** source: 11500365-Fig-3b
  *** xSgk1(61-431/S422D)[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1(61-431/S422D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: xSgk1 IP was pretreated with PP2A to remove prior phos
    *** inhibited by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2b
  *** xSgk1(61-431/S422D)[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1(61-431/S422D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: xSgk1 IP was pretreated with PP2A to remove prior phos
    *** inhibited by: PIFtide [addition]
    *** source: 11500365-Fig-3b

  *** xSgk1[phosAb] phos(T256)[phosAb] is detectable
    *** cells: WM35<xSgk1> in BMS
    *** partially inhibited by: xSgk1(S422A) [substitution]
    *** inhibited by: xSgk1(T256A) [substitution]
    *** unaffected by: xSgk1(F385A) [substitution]
    *** source: 18570873-Fig-5a

=================
Kinase Activation
=================

  *** xSgk1(61-415)[tAb]IP IVKA(Crosstide)[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** IPfrom: HEK293<xSgk1(61-415)> in BMS
    *** enhanced by: pHMSGK [addition]
    *** unaffected by: HMSGK [addition]
    *** enhanced by: pHMROK [addition]
    *** inhibited by: HMROK [addition]
    *** source: 12374740-Fig-6e
  *** xSgk1(61-431)[tAb]IP IVKA(Crosstide)[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** IPfrom: HEK293<xSgk1(61-431)> in BMS
    *** enhanced by: pHMSGK [addition]
    *** unaffected by: HMSGK [addition]
    *** enhanced by: pHMROK [addition]
    *** inhibited by: HMROK [addition]
    *** source: 12374740-Fig-6e
  *** xSgk1(S422D)[tAb]IP IVKA(Crosstide)[32P-ATP] is detectable
    *** cells: COS7<xSgk1(S422D)> in BMLS
    *** inhibited by: xSgk1(S422D/R147A) [substitution]
    *** source: 12374740-Fig-8a
  *** xSgk1(S422D)[tAb]IP phos(T256)[phosAb] is detectable
    *** cells: COS7<xSgk1(S422D)> in BMLS
    *** unaffected by: xSgk1(S422D/R147A) [substitution]
    *** source: 12374740-Fig-8b

===================
Protein Association
===================

  *** xPdpk1[tAb] copptby[WB] xSgk1(61-431/S422D)[GST] is detectable
    *** cells: HEK293<xPdpk1><xSgk1(61-431/S422D)> in BMS
    *** inhibited by: xSgk1(61-431) [substitution]
    *** source: 11500365-Fig-5b
