========================================
Semi-Direct phosphorylation on S422
========================================

Mtor IP

  *** rSgk1[32P] phos[32P-ATP] is increased by Mtor[Ab]IP
    *** cells: none
    *** IPfrom: WM35 in BMS
    *** inhibited by: rSgk1(S422A) [substitution]
    *** unaffected by: rSgk1(T256A) [substitution]
    *** source: 18570873-Fig-4d
  *** rSgk1[32P] phos[32P-ATP] is increased by Mtor[Ab]IP
    *** cells: none
    *** IPfrom: WM35 in BMS
    *** source: 18570873-Fig-4a
  *** rSgk1(61-431)[phosAb] phos(S422)[phosAb] is increased by Mtor[Ab]IP
    *** cells: none
    *** IPfrom: mEFs in BMS
    *** reqs: Rictor [KO]
    *** source: 18925875-Fig-4c

Rictor IP

  *** rSgk1(61-431)[phosAb] phos(S422)[phosAb] is increased by Rictor[Ab]IP
    *** cells: none
    *** IPfrom: HEK293 in BMS
    *** source: 18925875-Fig-3b
  *** rSgk1(61-431)[phosAb] phos(S422)[phosAb] is increased by Rictor[Ab]IP
    *** cells: none
    *** IPfrom: HEK293 in BMS
    *** source: 18925875-Fig-4e
  *** rSgk1(61-431)[phosAb] phos(S422)[phosAb] is increased by Rictor[Ab]IP
    *** cells: none
    *** IPfrom: HEK293 in BMS
    *** unaffected by: rPdpk1 [addition]
    *** source: 18925875-Fig-3c
  *** rSgk1(61-431)[phosAb] phos(S422)[phosAb] is increased by Rictor[Ab]IP
    *** cells: none
    *** IPfrom: mEFs in BMS
    *** reqs: Mlst8 [KO]
    *** source: 18925875-Fig-3e
  *** rSgk1(61-431)[phosAb] IVKA(peptide)[32P-ATP] is increased by Rictor[Ab]IP
    *** cells: none
    *** IPfrom: HEK293 in BMS
    *** enhanced by: rPdpk1 [addition]
    *** source: 18925875-Fig-3c

not Raptor IP

  *** rSgk1(61-431)[phosAb] phos(S422)[phosAb] is unchanged by Raptor[Ab]IP
    *** cells: none
    *** IPfrom: HEK293 in BMS
    *** source: 18925875-Fig-4e

=====================================================
Basal Phosphorylation on S422 reqs Mtorc2 components
=====================================================

Rictor--

  *** xSgk1(61-431)[phosAb] phos(S422)[phosAb] is detectable
    *** cells: mEFs<xSgk1(61-431)> in BMS
    *** reqs: Rictor [RNAi]
    *** source: 18925875-Fig-1a
  *** xSgk1(61-431)[tAb]IP IVKA(peptide)[32P-ATP] is detectable
    *** cells: mEFs<xSgk1(61-431)> in BMS
    *** reqs: Rictor [RNAi]
    *** source: 18925875-Fig-1a
  *** xSgk1(61-431)[tAb]IP IVKA(peptide)[32P-ATP] is detectable
    *** cells: mEFs<xSgk1(61-431)> in BMS
    *** reqs: Rictor [RNAi]
    *** source: 18925875-Fig-1b
  *** xSgk1[phosAb] phos(S422)[phosAb] is detectable
    *** cells: mEFs<xSgk1> in BMS
    *** reqs: Rictor [RNAi]
    *** source: 18925875-Fig-1c

Sin1--

  *** xSgk1(61-431)[phosAb] phos(S422)[phosAb] is detectable
    *** cells: mEFs<xSgk1(61-431)> in BMS
    *** reqs: Sin1 [RNAi]
    *** source: 18925875-Fig-1e

Mlst8--

  *** xSgk1(61-431)[phosAb] phos(S422)[phosAb] is detectable
    *** cells: mEFs<xSgk1(61-431)> in BMS
    *** reqs: Mlst8 [RNAi]
    *** source: 18925875-Fig-1d

========================================================
Basal Phosphorylation on S422 is unaffected by Rapamycin
========================================================

  *** xSgk1[phosAb] phos(S422)[phosAb] is detectable
    *** cells: HEK293<xSgk1> in BMS
    *** inhibited by: PI103 [pretreatment]
    *** unaffected by: Rapamycin [pretreatment]
    *** source: 18925875-Fig-5a
  *** xSgk1[phosAb] phos(S422)[phosAb] is detectable
    *** cells: HELA<xSgk1> in BMS
    *** inhibited by: PI103 [pretreatment]
    *** unaffected by: Rapamycin [pretreatment]
    *** source: 18925875-Fig-5c
  *** xSgk1[phosAb] phos(S422)[phosAb] is detectable
    *** cells: MCF7<xSgk1> in BMS
    *** inhibited by: PI103 [pretreatment]
    *** unaffected by: Rapamycin [pretreatment]
    *** source: 18925875-Fig-5b



==========================================
Association of Mtorc1 components with Sgk1
==========================================

  *** Mtor[Ab] copptby[WB] xSgk1[tAb] is detectable
    *** cells: WM35<xSgk1> in BMS
    *** unaffected by: xSgk1(S422A) [substitution]
    *** unaffected by: xSgk1(T256A) [substitution]
    *** unaffected by: xSgk1(F385A) [substitution]
    *** source: 18570873-Fig-5b
  *** Mtor[Ab] snaggedby[WB] rSgk1[GST] is detectable
    *** cells: WM35 in BMS
    *** unaffected by: rSgk1(S422A) [substitution]
    *** unaffected by: rSgk1(T256A) [substitution]
    *** unaffected by: rSgk1(F385A) [substitution]
    *** source: 18570873-Fig-5c
  *** xSgk1[tAb] copptby[WB] Mtor[Ab] is detectable
    *** cells: WM35<xSgk1> in BMS
    *** unaffected by: xSgk1(S422A) [substitution]
    *** unaffected by: xSgk1(T256A) [substitution]
    *** unaffected by: xSgk1(F385A) [substitution]
    *** source: 18570873-Fig-5b

  *** Pras40[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: WM35<xSgk1> in BMS
    *** unaffected by: xSgk1(S422A) [substitution]
    *** unaffected by: xSgk1(T256A) [substitution]
    *** unaffected by: xSgk1(F385A) [substitution]
    *** source: 18570873-Fig-5b
  *** Pras40[Ab] copptby[WB] xSgk1[tAb] is detectable
    *** cells: WM35<xSgk1> in BMS
    *** unaffected by: xSgk1(S422A) [substitution]
    *** unaffected by: xSgk1(T256A) [substitution]
    *** unaffected by: xSgk1(F385A) [substitution]
    *** source: 18570873-Fig-5b
  *** Raptor[Ab] copptby[WB] xSgk1[tAb] is detectable
    *** cells: WM35<xSgk1> in BMS
    *** unaffected by: xSgk1(S422A) [substitution]
    *** unaffected by: xSgk1(T256A) [substitution]
    *** unaffected by: xSgk1(F385A) [substitution]
    *** source: 18570873-Fig-5b
  *** Raptor[Ab] snaggedby[WB] rSgk1[GST] is detectable
    *** cells: WM35 in BMS
    *** unaffected by: rSgk1(S422A) [substitution]
    *** unaffected by: rSgk1(T256A) [substitution]
    *** unaffected by: rSgk1(F385A) [substitution]
    *** source: 18570873-Fig-5c
  *** Sin1[Ab] copptby[WB] xSgk1[tAb] is undetectable
    *** cells: WM35<xSgk1> in BMS
    *** unaffected by: xSgk1(S422A) [substitution]
    *** unaffected by: xSgk1(T256A) [substitution]
    *** unaffected by: xSgk1(F385A) [substitution]
    *** source: 18570873-Fig-5b

============================================
Mtorc2 components do not associate with Sgk1
============================================

  *** Rictor[Ab] copptby[WB] xSgk1[tAb] is undetectable
    *** cells: WM35<xSgk1> in BMS
    *** unaffected by: xSgk1(S422A) [substitution]
    *** unaffected by: xSgk1(T256A) [substitution]
    *** unaffected by: xSgk1(F385A) [substitution]
    *** source: 18570873-Fig-5b
  *** Rictor[Ab] snaggedby[WB] rSgk1[GST] is undetectable
    *** cells: WM35 in BMS
    *** unaffected by: rSgk1(S422A) [substitution]
    *** unaffected by: rSgk1(T256A) [substitution]
    *** unaffected by: rSgk1(F385A) [substitution]
    *** source: 18570873-Fig-5c

=========================================
Phosphorylation due to overexpressed Mtor
=========================================

  *** Sgk1[phosAb] phos(T256)[phosAb] is increased itpo xMtor
    *** cells: WM35 in BMS
    *** source: 18570873-Fig-3a
  *** Sgk1[phosAb] phos(T256)[phosAb] is increased itpo xMtor
    *** cells: WM35 in BMS
    *** source: 18570873-Fig-3b
