======================================================
Activation of Sgk1 requires phos on both T256 and T422
======================================================

phos(S422)

  *** xSgk1[GST]IP IVKA(peptide)[32P-ATP] is detectable
    *** cells: HEK293<xSgk1> in BMS
    *** inhibited by: xSgk1(S422A) [substitution]
    *** enhanced by: xSgk1(S422D) [substitution]
    *** source: 10191262-Fig-10a
  *** xSgk1[GST]IP IVKA(peptide)[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: xSgk1 IP was pretreated with PP2A to remove prior phos
    *** enhanced by: xSgk1(S422D) [substitution]
    *** source: 10191262-Fig-5d
  *** xSgk1(61-431)[GST]IP IVKA(peptide)[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1(61-431)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: xSgk1 IP was pretreated with PP2A to remove prior phos
    *** inhibited by: xPdpk1(L155E) [substitution]
    *** enhanced by: xSgk1(61-431/S422D) [substitution]
    *** source: 11500365-Fig-2b
  *** xSgk1(61-431)[GST]IP IVKA(peptide)[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1(61-431)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: xSgk1 IP was pretreated with PP2A to remove prior phos
    *** inhibited by: PIFtide [addition]
    *** enhanced by: xSgk1(61-431/S422D) [substitution]
    *** source: 11500365-Fig-3b
  *** xSgk1(61-431/S422D)[GST]IP IVKA(peptide)[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1(61-431/S422D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: xSgk1 IP was pretreated with PP2A to remove prior phos
    *** inhibited by: xPdpk1(L155E) [substitution]
    *** inhibited by: xSgk1(61-431) [substitution]
    *** source: 11500365-Fig-2b
  *** xSgk1(61-431/S422D)[GST]IP IVKA(peptide)[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1(61-431/S422D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: xSgk1 IP was pretreated with PP2A to remove prior phos
    *** inhibited by: PIFtide [addition]
    *** inhibited by: xSgk1(61-431) [substitution]
    *** source: 11500365-Fig-3b
  *** xSgk1(61-431/S422D)[GST]IP IVKA(peptide)[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1(61-431/S422D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** inhibited by: xPdpk1(K155E) [substitution]
    *** inhibited by: xPdpk1(K155A) [substitution]
    *** inhibited by: xPdpk1(K115A) [substitution]
    *** inhibited by: xPdpk1(I119A) [substitution]
    *** inhibited by: xPdpk1(Q150A) [substitution]
    *** unaffected by: xPdpk1(A277V) [substitution]
    *** source: 11500365-Fig-4b
  *** xSgk1(61-431)[tAb]IP IVKA(peptide)[32P-ATP] is detectable
    *** cells: mEFs<xSgk1(61-431)> in BMS
    *** inhibited by: xSgk1((61-431)/S442A) [substitution]
    *** enhanced by: xSgk1((61-431)/S442D) [substitution]
    *** source: 18925875-Fig-1b
  *** xSgk1(61-431)[tAb]IP IVKA(peptide)[32P-ATP] is detectable
    *** cells: mEFs<xSgk1(61-431)> in BMS
    *** inhibited by: xSgk1((61-431)/S442A) [substitution]
    *** source: 18925875-Fig-1a

phos(T256)

  *** xSgk1[GST]IP IVKA(peptide)[32P-ATP] is detectable
    *** cells: HEK293<xSgk1> in BMS
    *** inhibited by: xSgk1(T256A) [substitution]
    *** source: 10191262-Fig-7a
  *** xSgk1[GST]IP IVKA(peptide)[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xSgk1> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: xSgk1 IP was pretreated with PP2A to remove prior phos
    *** inhibited by: xSgk1(K127A) [substitution]
    *** inhibited by: xSgk1(T256A) [substitution]
    *** partially inhibited by: xSgk1(S422A) [substitution]
    *** inhibited by: xSgk1(T256A/S422A) [substitution]
    *** source: 10191262-Fig-5b
  *** rSgk1(61-431)[phosAb] IVKA(peptide)[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** IPfrom: HEK293 in BMS
    *** enhanced by: Rictor[Ab]IP [addition]
    *** source: 18925875-Fig-3c

============================================
Activation in response to overexpressed Pdk1
============================================

  *** xSgk1[GST]IP IVKA(peptide)[32P-ATP] is increased itpo xPdk1
    *** cells: HEK293<xSgk1> in BMS
    *** enhanced by: Igf1 (10 min) [stim]
    *** source: 10191262-Fig-9

