========================
Semi-direct GAP activity
========================

  *** rRheb GTP-hydrolysis[32P-GTP] is increased by (xTsc1[tAb]IP + xTsc2[tAb]IP)
    *** cells: none
    *** IPfrom: HEK293<xTsc1><xTsc2> in BMS
    *** source: 12869586-Fig-1b
  *** rRheb GTP-hydrolysis[32P-GTP] is increased by (xTsc1[tAb]IP + xTsc2[tAb]IP)
    *** cells: none
    *** IPfrom: HEK293<xTsc1><xTsc2> in BMS
    *** source: 12869586-Fig-1c
  *** rRheb GTP-hydrolysis[32P-GTP] is increased by (xTsc1[tAb]IP + xTsc2[tAb]IP)
    *** cells: none
    *** IPfrom: HEK293<xTsc1><xTsc2> in BMS
    *** inhibited by: xTsc2(1-1007) [substitution]
    *** inhibited by: xTsc2(1008-1765) [substitution]
    *** comment: aa numbers NOT converted to Uniprot human canonical sequence
    *** source: 12869586-Fig-1d
  *** rRheb GTP-hydrolysis[32P-GTP] is increased by (xTsc1[tAb]IP + xTsc2[tAb]IP)
    *** cells: none
    *** IPfrom: HEK293<xTsc1><xTsc2> in BMS
    *** reqs: xTsc2 [omission]
    *** source: 12869586-Fig-1e

=====================================================
Decrease in GTP-association by coexpression with Tsc2 
=====================================================

  *** xRheb[tAb]IP GTP-association[TLC] is decreased itpo xTsc2
    *** cells: DROSOPHILAS2 in BMS
    *** enhanced by: xTsc1 [addition]
    *** source: 12771962-Fig-1a
  *** xRheb[tAb]IP GTP-association[TLC] is decreased itpo xTsc2
    *** cells: HEK293T in BMLS
    *** unaffected by: xTsc1 [addition]
    *** source: 12842888-Fig-3
  *** xRheb[tAb]IP GTP-association[TLC] is decreased itpo xTsc2
    *** cells: HEK293 in BMS
    *** unaffected by: xTsc1 [addition]
    *** unaffected by: xTsc2(400-1745)"Tsc1-binding-mut" [substitution]
    *** inhibited by: xTsc2(600-1745) [addition]
    *** inhibited by: xTsc2(700-1745) [addition]
    *** inhibited by: xTsc2(800-1745) [addition]
    *** inhibited by: xTsc2(1007-1745) [addition]
    *** source: 15340059-Fig-4b
  ------------------------------------------------------------------------
  *** xRheb[tAb]IP GTP-association[TLC] is decreased itpo (xTsc1 + xTsc2)
    *** cells: HEK293 in BMS
    *** inhibited by: xTsc1 + xTsc2(R1701P) [substitution]
    *** inhibited by: xTsc1 + xTsc2(R1703P) [substitution]
    *** unaffected by: xTsc1 + xTsc2(R1707Q/R1709Q) [substitution]
    *** inhibited by: xTsc1 + xTsc2(K1595Q/K1596Q/K1597Q) [addition]
    *** source: 15340059-Fig-5a
  *** xRheb[tAb]IP GTP-association[TLC] is decreased itpo (xTsc1 + xTsc2)
    *** cells: DROSOPHILAS2 in BMS
    *** inhibited by: xTsc2(K1638A) [substitution]
    *** inhibited by: xTsc2(N1643K) [substitution]
    *** source: 12771962-Fig-1c
  *** xRheb[tAb]IP GTP-association[TLC] is decreased itpo (xTsc1 + xTsc2)
    *** cells: HEK293 in BMS
    *** unaffected by: xTsc1 + xTsc2(R611Q) [substitution]
    *** unaffected by: xTsc1 + xTsc2(E814K) [substitution]
    *** unaffected by: xTsc1 + xTsc2(R1156W) [substitution]
    *** partially inhibited by: xTsc1 + xTsc2(N1601K) [substitution]
    *** inhibited by: xTsc1 + xTsc2(N1609S) [substitution]
    *** inhibited by: xTsc1 + xTsc2(N1639S) [substitution]
    *** source: 15340059-Fig-5a
  *** xRheb[tAb]IP GTP-association[TLC] is decreased itpo (xTsc2 + xTsc1)
    *** cells: HEK293 in BMLS
    *** inhibited by: xTsc2(1-1007) [substitution]
    *** source: 12869586-Fig-1f
  *** xRheb[tAb]IP GTP-association[TLC] is decreased itpo (xTsc1 + xTsc2)
    *** cells: HEK293 in BMS
    *** source: 15340059-Fig-1b
  *** xRheb[tAb]IP GTP-association[TLC] is decreased itpo (xTsc1 + xTsc2)
    *** cells: HEK293 in BMS
    *** source: 15340059-Fig-2a

==============================================
Increase in GTP-association by removal of Tsc2 
==============================================

  *** xRheb[tAb]IP GTP-association[TLC] is increased itao Tsc2 [RNAi]
    *** cells: DROSOPHILAS2 in BMS
    *** source: 12771962-Fig-1d

==============================
Rheb-Tsc2 Protein Association
==============================

  *** xTsc2(965-1807)[tAb] snaggedby[WB] rRheb{GMPPNP}[GST] is detectable
    *** cells: HEK293T<xTsc2(965-1807)> in BMS
    *** source: 12842888-Fig-2
  *** rTsc2(1531-1807)[tAb] boundby[WB] rRheb{NucFree}[GST] is detectable
    *** cells: none
    *** inhibited by: rRheb{GDP} [substitution]
    *** unaffected by: rRheb{GMPPNP} [substitution]
    *** source: 15854902-Fig-5a

  *** Tsc2[Ab] copptby[WB] xRheb[GST] is detectable
    *** cells: HEK293T<xRheb> in BMS
    *** enhanced by: xRheb(T38M) [substitution]
    *** partially inhibited by: xRheb(I39K) [substitution]
    *** partially inhibited by: xRheb(Q64L) [substitution]
    *** unaffected by: xRheb(S20N) [substitution]
    *** source: 15854902-Fig-2b
  *** xTsc2[tAb] copptby[WB] xRheb[tAb] is undetectable
    *** cells: HEK293T<xRheb><xTsc2> in BMS
    *** unaffected by: xTsc1 [addition]
    *** source: 15854902-Fig-4b






