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Rgl1 is Hras effector
Direct interaction between Hras{GTP} and Rgl1
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  *** rHras{GTPgS}[35S] boundby[WB] rRgl1(602-768)[Resin] is detectable
    *** cells: none
    *** inhibited by: rHras{GDP} [substitution]
    *** source: 7498475-Fig-1
  *** rHras{GTPgS}[35S] boundby[WB] rRgl1(602-768)[Resin] is detectable
    *** cells: none
    *** inhibited by: rRap1a{GTPgS} [addition]
    *** source: 7498475-Fig-2b
  *** rHras{GTPgS}[35S] boundby[WB] rRgl1(602-768)[Resin] is detectable
    *** cells: none
    *** source: 7498475-Fig-2a
  *** rHras{GTPgS}[35S] boundby[WB] rRgl1(602-768)[Resin] is detectable
    *** cells: none
    *** unaffected by: rRgl1(602-768){phos} [substitution]
    *** comment: rRgl1(602-768) was phosed by pPKA
    *** source: 7498475-Fig-3
  *** rHras(G12V){GTPgS}[35S] boundby[WB] rRgl1(602-768)[Resin] is detectable
    *** cells: none
    *** inhibited by: rHras(G12V/T35S){GTPgS} [substitution]
    *** partially inhibited by: rHras(G12V/E37G){GTPgS} [substitution]
    *** inhibited by: rHras(G12V/Y40C){GTPgS} [substitution]
    *** source: 9099691-Fig-3a
  *** rHras(G12V){GTPgS}[35S] boundby[WB] rRgl1(602-768)[Resin] is detectable
    *** cells: none
    *** inhibited by: rRaf1(1-149) [addition]
    *** source: 9099691-Fig-3b
  *** rHras(G12V/E37G){GTPgS}[35S] boundby[WB] rRgl1(602-768)[Resin] is detectable
    *** cells: none
    *** unaffected by: rRaf1(1-149) [addition]
    *** source: 9099691-Fig-3b

  *** xRgl1[tAb] copptby[WB] xHras(G12V)"CA"[tAb] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xHras(G12V/D38A) [substitution]
    *** inhibited by: xHras(G12V/T35S) [substitution]
    *** partially inhibited by: xHras(G12V/E37G) [substitution]
    *** partially inhibited by: xHras(G12V/Y40C) [substitution]
    *** inhibited by: xHras(G12V/D38E) [substitution]
    *** source: 15143186-Fig-6

  *** xRgl1[tAb] copptby[WB] xHras(G12V)[Ab] is detectable
    *** cells: COS in BMS
    *** inhibited by: xHras(G12V/T35S) [substitution]
    *** unaffected by: xHras(G12V/E37G) [substitution]
    *** inhibited by: xHras(G12V/Y40C) [substitution]
    *** source: 9099691-Fig-2b
  *** xRgl1[tAb] copptby[WB] xHras(G12V)[Ab] is detectable
    *** cells: SF9 in BMS
    *** inhibited by: xHras(S17N) [substitution]
    *** source: 9099691-Fig-1b
  *** xRgl1[tAb] copptby[WB] xHras(G12V)[Ab] is detectable
    *** cells: SF9 in BMS
    *** inhibited by: xRgl1(1-505) [substitution]
    *** inhibited by: xRgl1(85-505) [substitution]
    *** source: 9099691-Fig-1c

==========================
Rgl1 is not a Gef for Hras
==========================

  *** xHras[tAb]IP GTP-association[35S-GTPgS] is unchanged by rRgl1
    *** cells: none
    *** IPfrom: SF9 in BMS "cytosolic-fraction"
    *** source: 9099691-Fig-5
  *** xHras[tAb]IP GTP-association[35S-GTPgS] is unchanged by rRgl1
    *** cells: none
    *** IPfrom: SF9 in BMS "membrane-fraction"
    *** source: 9099691-Fig-5






