=================================================
Tyrosine phosphorylation in the presence of xCrkI
=================================================

  *** xRapGef1[Ab]IP Yphos[pYAb] is increased itpo xCrkI
    *** cells: COS1 in BMS
    *** inhibited by: xCrkI(R38V) [substitution]
    *** source: 10318861-Fig-2b
  *** xRapGef1[Ab]IP Yphos[pYAb] is increased itpo xCrkI
    *** cells: COS1 in BMS
    *** inhibited by: xRapGef1(Y504F) [substitution]
    *** partially inhibited by: xRapGef1(Y554F/Y561F/Y570F) [substitution]
    *** unaffected by: xRapGef1(Y478F/Y485F/Y579F) [substitution]
    *** source: 10318861-Fig-7a
  *** xRapGef1[Ab]IP Yphos[pYAb] is increased itpo xCrkI
    *** cells: COS1 in BMS
    *** unaffected by: xRapGef1(mnr)"farnesylated" [substitution]
    *** source: 10318861-Fig-1c
  *** xRapGef1[Ab]IP Yphos[pYAb] is increased itpo xCrkI
    *** cells: HEK293 in BMS
    *** unaffected by: xRapGef1(391-1077) [substitution]
    *** inhibited by: xRapGef1(580-1077) [substitution]
    *** source: 10318861-Fig-5[t]
  *** xRapGef1[GST]IP Yphos[pYAb] is increased itpo xCrkI
    *** cells: COS1 in BMS
    *** source: 10318861-Fig-2c
  *** xRapGef1[GST]IP Yphos[pYAb] is increased itpo xCrkI(mnr)"vCrk"
    *** cells: 3Y1 in BMS
    *** comment: lysates were denatured before IP to remove coppt proteins
    *** source: 10318861-Fig-3b
  *** xRapGef1[GST]IP Yphos[pYAb] is increased itpo xCrkI(mnr)"vCrk"
    *** cells: 3Y1 in BMS
    *** source: 10318861-Fig-3a

====================
Physical interaction
====================

  *** rRapGef1[Ab] boundby[WB] rCrkI[GST] is detectable
    *** cells: none
    *** source: 9268367-Fig-5b
  *** xRapGef1[Ab] copptby[WB] xCrkI[Ab] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: xRapGef1(391-1077) [substitution]
    *** unaffected by: xRapGef1(580-1077) [substitution]
    *** source: 10318861-Fig-5
  *** rCrkI[GST] boundby[WB] rRapGef1[Ab] is detectable
    *** cells: none
    *** source: 9268367-Fig-5b

  *** xCrkI[Ab] copptby[WB] xRapGef1[Ab] is detectable
    *** cells: RK in BMS
    *** source: 7512734-Fig-4
  *** xCrkI[Ab] copptby[WB] xRapGef1[Ab] is detectable
    *** cells: COS1 in BMS
    *** inhibited by: xCrkI(R38V) [substitution]
    *** source: 10318861-Fig-2b




