========================
RalGds is a Gef for Rala
========================

  *** rRala GDP-dissociation[3H-GDP] is increased by rRalGds
    *** cells: none
    *** source: 8094051-Fig-5
  *** rRala[GST] GDP-dissociation[3H-GDP] is increased by rRalGds
    *** cells: none
    *** enhanced by: rPdpk1 [addition]
    *** source: 11889038-Fig-4a

*** not Akt1--

  *** xRala[GST]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: HEK293 in BMS
    *** unaffected by: xAkt1(mnr)"CA" [addition]
    *** source: 11889038-Fig-3a

============================================
Rala{GTP} itpo RalGds is enhanced itpo Hras--
============================================

  *** xRala[His]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: COS7 in BMS
    *** enhanced by: xHras(Q61L) [addition]
    *** source: 8631302-Fig-3
  *** xRala[tAb]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: COS7 in BMLS
    *** enhanced by: xHras(G12V)"CA" [addition]
    *** source: 11283245(D)
  *** xRala[GST]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: HEK293 in BMS
    *** enhanced by: xHras(L61Q)"CA" [addition]
    *** source: 11889038-Fig-3a
  *** xRala[tAb]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: COS1 in BMS
    *** enhanced by: xHras(G12V)"CA" [addition]
    *** unaffected by: xHras(G12V/mnr)"membrane-binding-mut" [addition]
    *** source: 9416833-Fig-1b
  *** xRala[tAb]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: COS7 in BMLS
    *** enhanced by: xHras(G12V)"CA" [addition]
    *** source: 11748241-Fig-1

*** Other Ras Family Members

  *** xRala[tAb] GTP-association[BDPD] is increased itpo xRalGds
    *** cells: HEK293T in BMS
    *** enhanced by: xNras(G12V)"CA" [addition]
    *** enhanced by: xKras(G12V)"CA" [addition]
    *** enhanced by: xMras(Q71L)"CA" [addition]
    *** enhanced by: xRras(G38V)"CA" [addition]
    *** enhanced by: xRras2(G23V)"CA" [addition]
    *** enhanced by: xRit1(Q79L)"CA" [addition]
    *** comment: ranked in order of size of effect
    *** source: 15143186-Fig-2a

*** Pdpk1--

  *** xRala[GST]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: HEK293 in BMS
    *** enhanced by: xPdpk1 [addition]
    *** enhanced by: xPdpk1(K11N)"KD" [addition]
    *** enhanced by: xPdpk1(mnr)"PH-domain-mut" [addition]
    *** unaffected by: xPdpk1(del(1-50))"Cterm-mut" [addition]
    *** source: 11889038-Fig-5a
  *** xRala[GST]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: HEK293 in BMS
    *** enhanced by: xPdpk1(mnr)"CA" [addition]
    *** source: 11889038-Fig-3a

*** Pi3k--

  *** xRala[GST]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: HEK293 in BMS
    *** enhanced by: xPik3c?(mnr)"CA" [addition]
    *** source: 11889038-Fig-3a

*** PMA--

  *** xRala[tAb]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: COS7 in BMLS
    *** inhibited by: PMA (30 min) [stim]
    *** source: 11283245(D)

========================================
Rala{GTP} itpo RalGds is not enhanced by:
========================================

*** Rap1a--

  *** xRala[His]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: COS7 in BMS
    *** unaffected by: xRap1a(Q63E) [addition]
    *** source: 8631302-Fig-3
  *** xRala[tAb]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: COS1 in BMS
    *** unaffected by: xRap1a(Q63E)"CA" [addition]
    *** source: 9416833-Fig-1b
  *** xRala[tAb] GTP-association[BDPD] is increased itpo xRalGds
    *** cells: HEK293T in BMS
    *** enhanced by: xRap1a(G12V)"CA" [addition]
    *** source: 15143186-Fig-2a

*** not Rap2a--

  *** xRala[tAb] GTP-association[BDPD] is increased itpo xRalGds
    *** cells: HEK293T in BMS
    *** unaffected by: xRap2a(G12V)"CA" [addition]
    *** source: 15143186-Fig-2a

*** not Rin1--

  *** xRala[tAb] GTP-association[BDPD] is increased itpo xRalGds
    *** cells: HEK293T in BMS
    *** unaffected by: xRin1(A78L) [addition]
    *** source: 15143186-Fig-2a

*** not Rras--

  *** xRala[His]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: COS7 in BMS
    *** unaffected by: xRras(G38V) [addition]
    *** source: 8631302-Fig-3

===========================
required RalGds attributes
===========================

  *** xRala[tAb]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: COS1 in BMS
    *** unaffected by: xRalGds(C203S)"membrane-binding-mut" [substitution]
    *** source: 9416833-Fig-1b
  *** xRala[tAb]IP GTP-association[TLC] is detectable
    *** cells: COS7<xRalGds> in BMLS
    *** enhanced by: xRalGds(CAAX) [substitution]
    *** inhibited by: xRalGds(del(RBD)) [substitution]
    *** source: 11748241-Fig-3a
  *** xRala[His]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: COS7 in BMS
    *** inhibited by: xRalGds(1-784)"RA-domain-mutant" [substitution]
    *** source: 8631302-Fig-3

=========
no extras
=========

  *** xRala[tAb]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: HEK293 in BMS
    *** source: 10896938-Fig-1a
  *** xRala[GST]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: HEK293 in BMS
    *** source: 11889038-Fig-3a
  *** xRala[His]IP GTP-association[TLC] is increased itpo xRalGds
    *** cells: COS7 in BMS
    *** source: 8631302-Fig-2





