IVKA

  *** xRaf1[tAb]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased itpo xMras(H81L)"CA"
    *** cells: HEK293T in BMS
    *** enhanced by: xShoc2 [addition]
    *** source: 16630891-Fig-2b
  *** xRaf1[tAb]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased itpo xMras(H81L)"CA"
    *** cells: HEK293T in BMS
    *** enhanced by: xShoc2 [addition]
    *** enhanced by: xRaf1(S259A) [substitution]
    *** source: 16630891-Fig-4a
  *** xRaf1[tAb]IP IVKA(Mek1,Erk2)[32P-ATP] is increased itpo xMras(Q71L)"CA"
    *** cells: HEK293T in BMS
    *** source: 15143186-Fig-1a

Mras-CA coppt

  *** xRaf1[tAb] copptby[WB] xMras(Q71L)"CA"[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 15143186-Fig-1e
  *** Raf1[MS] copptby[WB] xMras(H81L)"CA"[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 16630891-Fig-1a
  *** Raf1[Ab] copptby[WB] xMras[GST] is detectable
    *** cells: HEK293T in BMS
    *** enhanced by: xMras(Q71L)"CA" [substitution]
    *** source: 16630891-Fig-1b,1f
  *** Raf1[Ab] copptby[WB] xMras(H81L)"CA"[GST] is detectable
    *** cells: HEK293T in BMS
    *** partially reqs: Shoc2 [RNAi]
    *** source: 16630891-Fig-1f
  *** xRaf1[tAb] copptby[WB] xMras(H81L)"CA"[GST] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xMras(WT) [substitution]
    *** source: 16630891-Fig-3a,3d
  *** xRaf1[tAb] copptby[WB] xMras(H81L)"CA"[tAb] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xShoc2 [addition]
    *** source: 16630891-Fig-3b,3c
  *** xRaf1[tAb] copptby[WB] xMras(H81L)"CA"[tAb] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xKsr1 [addition]
    *** unaffected by: xCnksr1 [addition]
    *** source: 16630891-Fig-3d
  *** xRaf1[tAb] copptby[WB] xMras(H81L)"CA"[tAb] is detectable
    *** cells: HEK293T in BMS
    *** enhanced by: xShoc2 [addition]
    *** enhanced by: xRaf1(S259A) [substitution]
    *** inhibited by: xRaf1(R89L)"Ras-binding-mut" [substitution]
    *** enhanced by: xRaf1(S259A) [substitution]
    *** unaffected by: xRaf1(S338A/Y341F) [substitution]
    *** enhanced by: xRaf1(S338D/Y341D) [substitution]
    *** unaffected by: xRaf1(S491A/S494A) [substitution]
    *** unaffected by: xRaf1(S491D/S494D) [substitution]
    *** source: 16630891-Fig-4b
  *** xRaf1[tAb] copptby[WB] xMras(H81L)"CA"[tAb] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xShoc2 [addition]
    *** unaffected by: xNras(G12V)"CA" [addition]
    *** source: 16630891-Fig-6b

phos(S259)

  *** xRaf1[phosAb] copptby[WB] xMras(H81L)"CA"[tAb] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xShoc2 [addition]
    *** comment: Coppt Raf1 is phosed on S259[phosAb]
    *** source: 16630891-Fig-3a
  *** xRaf1[phosAb] copptby[WB] xMras(H81L)"CA"[tAb] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xShoc2 [addition]
    *** unaffected by: xNras(G12V)"CA" [addition]
    *** comment: Coppt Raf1 is phosed on S259[phosAb]
    *** source: 16630891-Fig-3b
  *** xRaf1[phosAb] copptby[WB] xMras(H81L)"CA"[tAb] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xShoc2 [addition]
    *** inhibited by: xShoc2(C260Y) [addition]
    *** unaffected by: xShoc2(D175N) [addition]
    *** partially inhibited by: xShoc2(D175N) [addition]
    *** inhibited by: xShoc2(P510Y) [addition]
    *** comment: Coppt Raf1 is phosed on S259[phosAb]
    *** source: 16630891-Fig-3c
  *** xRaf1[phosAb] copptby[WB] xMras(H81L)"CA"[tAb] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xShoc2 [addition]
    *** unaffected by: xCnksr1 [addition]
    *** unaffected by: xKsr1 [addition]
    *** comment: Coppt Raf1 is phosed on S259[phosAb]
    *** source: 16630891-Fig-3d
  *** xRaf1[phosAb] copptby[WB] xMras(H81L)"CA"[tAb] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xShoc2 [addition]
    *** inhibited by: xRaf1(S259A) [substitution]
    *** enhanced by: xRaf1(S259A) [substitution]
    *** enhanced by: xRaf1(S338A/Y341F) [substitution]
    *** enhanced by: xRaf1(S338D/Y341D) [substitution]
    *** enhanced by: xRaf1(S491A/S494A) [substitution]
    *** unaffected by: xRaf1(S491D/S494D) [substitution]
    *** comment: Coppt Raf1 is phosed on S259[phosAb]
    *** source: 16630891-Fig-4b
  *** xRaf1[tAb]IP phos(S259)[phosAb] is decreased itpo xShoc2
    *** cells: HEK293T<xMras(H81L)"CA"> in BMS
    *** source: 16630891-Fig-3b
  *** xRaf1[tAb]IP phos(S259)[phosAb] is unchanged itpo xShoc2
    *** cells: HEK293T in BMS
    *** source: 16630891-Fig-3b

phos(S338)

  *** xRaf1[phosAb] copptby[WB] xMras(H81L)"CA"[tAb] is detectable
    *** cells: HEK293T in BMS
    *** enhanced by: xShoc2 [addition]
    *** enhanced by: xCnksr1 [addition]
    *** unaffected by: xKsr1 [addition]
    *** comment: Coppt Raf1 is phosed on S338[phosAb]
    *** source: 16630891-Fig-3a,3b

