============================================
Direct Interaction between Hras{GTP} and Raf1
============================================

  *** rHras{GTPgS}[35S] boundby[WB] rRaf1(1-257)[Resin] is detectable
    *** cells: none
    *** partially inhibited by: rRaf1(1-257){phos} [substitution]
    *** comment: rRgl1(602-768) was phosed by pPKA
    *** source: 7498475-Fig-3

========================================
Raf1 activity is increased itpo Hras{GTP}
========================================

  *** xRaf1[tAb]IP IVKA(Mek?,Erk?,MBP)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS7 in BMS
    *** source: 8999998-Fig-2a
  *** xRaf1[tAb]IP IVKA(Mek?,Erk2)[32P-ATP] is increased by xHras(G12V)"CA"
    *** cells: HEK293T in BMS
    *** source: 15143186-Fig-1a
  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,MBP)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** source: 9727023-Fig-2b
  *** xRaf1[tAb]IP IVKA(Mek1)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: HEK293 in BMLS
    *** source: 9351825-Fig-5c
  *** Raf1[Ab]IP IVKA(snr)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS7 in BMS
    *** source: 9234708-Fig-7
  *** xRaf1[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS1 in BMLS "containing mevalonolactone"
    *** source: 10358073-Fig-1a,b
  *** xRaf1[Ab]IP IVKA(snr)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** inhibited by: xHras(G12V/E37G) [substitution]
    *** source: 12077328-Fig-4a
  *** xRaf1[tAb]IP IVKA(Mek?,Erk?,MBP)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS7 in BMS
    *** source: 16364920-Fig-S1
  *** xRaf1[tAb]IP IVKA(Mek1)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: HEK293 in BMLS
    *** source: 11325826-Fig-1d
  *** xRaf1[tAb]IP IVKA(Mek1)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: HEK293<xBraf> in BMLS
    *** source: 11325826-Fig-1b
  *** Raf1[Ab]IP IVKA(Mek1,Erk1)[32P-ATP] is increased itpo xHras(Q61L)"CA"
    *** cells: NIH3T3 in BMS
    *** source: 8887643-Fig-7
  *** xRaf1[GST]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased itpo xHras(Q61L)"CA"
    *** cells: COS in BMLS
    *** source: 15632082-Fig-4c

====================================================
Raf1 activity itpo Hras{GTP} requires Raf1-phos(S388)
====================================================

  *** xRaf1[tAb]IP IVKA(Mek?,Erk?,MBP)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS7 in BMS
    *** inhibited by: xRaf1(S338A) [substitution]
    *** source: 16364920-Fig-3b
  *** xRaf1[tAb]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased itpo xHras(G12R)"CA"
    *** cells: COS in BMS
    *** inhibited by: xRaf1(S338A) [substitution]
    *** source: 10205168-Fig-3c
  *** xRaf1[Ab]IP IVKA(snr)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS7 in BMS
    *** inhibited by: xRaf1(S338A/S339A) [substitution]
    *** source: 9234708-Fig-7
  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,Elk1)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: HEK293 in BMS
    *** inhibited by: xRaf1(S338A) [substitution]
    *** unaffected by: xRaf1(S338D) [substitution]
    *** source: 11447113-Fig-3a

============================================
Raf1 is phosphorylated on S338 itpo Hras{GTP}
============================================

  *** xRaf1[tAb]IP phos(S338)[phosAb] is increased itpo xHras(G12R)"CA"
    *** cells: COS in BMS
    *** inhibited by: xRaf1(R89L)"Ras-binding-mut" [substitution]
    *** source: 10205168-Fig-3b
  *** xRaf1[tAb]IP phos(S338)[phosAb] is increased itpo xHras(G12V)"CA"
    *** cells: COS1 in BMS
    *** inhibited by: xRaf1(S259A) [substitution]
    *** source: 11782426-Fig-5
  *** xRaf1[Ab] phos(S338)[phosAb-ppt] is increased itpo xHras(G12V)"CA"
    *** cells: COS7 in BMS
    *** source: 9823899-Fig-4c
  *** xRaf1[tAb]IP phos(S338)[phosAb] is increased itpo xHras(G12V)"CA"
    *** cells: COS7 in BMS
    *** source: 16364920-Fig-3c
  *** xRaf1[tAb]IP phos(S338)[phosAb] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** inhibited by: xHras(G12V/E37G) [substitution]
    *** source: 12077328-Fig-5c
  *** Raf1[phosAb] phos(S338)[phosAb] is increased itpo xHras(Q61L)"CA"
    *** cells: PC6 in BMLS
    *** source: 15632082-Fig-1c
  *** Raf1[phosAb] phos(S338)[phosAb] is increased itpo xRit1
    *** cells: PC6 in BMLS
    *** unaffected by: xRit1(Q79L)"CA" [substitution]
    *** source: 15632082-Fig-1c
  *** xRaf1[tAb]IP phos(S338)[phosAb] is increased itpo xHras(Q61L)"CA"
    *** cells: COS in BMS
    *** source: 15632082-Fig-4a
  *** xRaf1[GST]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased itpo xHras(Q61L)
    *** cells: COS<xRaf1> in BMS
    *** source: 16157584-Fig-5d
  *** xRaf1[GST]IP phos(S338)[phosAb] is increased itpo xHras(Q61L)
    *** cells: COS<xRaf1> in BMS
    *** source: 16157584-Fig-5d

====================================================
Raf1 activity itpo Hras{GTP} requires Raf1-phos(S621)
====================================================

  *** xRaf1[tAb]IP IVKA(Mek?,Erk?,MBP)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS7 in BMS
    *** inhibited by: xRaf1(S621A) [substitution]
    *** source: 16364920-Fig-3d

==========================================================
Raf1 activity itpo Hras{GTP} requires Raf1-phos(T491/S494)
==========================================================

  *** xRaf1[tAb]IP IVKA(Mek?,Erk?,MBP)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS7 in BMS
    *** inhibited by: xRaf1(T491A/S494A) [substitution]
    *** source: 16364920-Fig-3d
  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,Elk1)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: HEK293 in BMS
    *** partially inhibited by: xRaf1(T491A/S494A) [substitution]
    *** unaffected by: xRaf1(T491E/S494D) [substitution]
    *** source: 11447113-Fig-2a

============================================
Raf1 is phosphorylated on S491 itpo Hras{GTP}
============================================

  *** xRaf1[phosAb] phos(T491)[phosAb] is increased itpo xHras(G12V)"CA"
    *** cells: HEK293 in BMS
    *** inhibited by: xRaf1(T491A) [substitution]
    *** unaffected by: xRaf1(S494A) [substitution]
    *** source: 11447113-Fig-2c

============================================
Raf1 is phosphorylated on S494 itpo Hras{GTP}
============================================

  *** xRaf1[phosAb] phos(S494)[phosAb] is increased itpo xHras(G12V)"CA"
    *** cells: HEK293 in BMS
    *** inhibited by: xRaf1(S494A) [substitution]
    *** unaffected by: xRaf1(T491A) [substitution]
    *** source: 11447113-Fig-2e

====================================================
Raf1 activity itpo Hras{GTP} requires Raf1-phos(Y341)
====================================================

  *** xRaf1[tAb]IP IVKA(Mek?,Erk?,MBP)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS7 in BMS
    *** inhibited by: xRaf1(Y341A) [substitution]
    *** source: 16364920-Fig-3a
  *** xRaf1[tAb]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased itpo xHras(G12R)"CA"
    *** cells: COS in BMS
    *** inhibited by: xRaf1(Y341A) [substitution]
    *** source: 10205168-Fig-3c
  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,Elk1)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: HEK293 in BMS
    *** inhibited by: xRaf1(Y341F) [substitution]
    *** inhibited by: xRaf1(Y341D) [substitution]
    *** inhibited by: xRaf1(S338A/Y341F) [substitution]
    *** bkg inhibited by: xRaf1(S338D/Y341D) [substitution]
    *** source: 11447113-Fig-3b

============================================================
Raf1 activity itpo Hras{GTP} does not require Raf1-phos(S257)
============================================================

  *** xRaf1[Ab]IP IVKA(snr)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** unaffected by: xRaf1(S257L) [substitution]
    *** source: 12077328-Fig-4a
  *** xRaf1[Ab]IP IVKA(snr)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** unaffected by: xRaf1(S257L) [substitution]
    *** source: 12077328-Fig-8a
  *** xRaf1[tAb]IP phos(S338)[phosAb] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** unaffected by: xRaf1(S257L) [substitution]
    *** source: 12077328-Fig-5c

============================================================
Raf1 activity itpo Hras{GTP} does not require Raf1-phos(S259)
============================================================

  *** xRaf1[Ab]IP IVKA(snr)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** unaffected by: xRaf1(S259A) [substitution]
    *** source: 12077328-Fig-4a
  *** xRaf1[Ab]IP IVKA(snr)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** unaffected by: xRaf1(S259A) [substitution]
    *** source: 12077328-Fig-8a
  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,MBP)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS1 in BMS
    *** unaffected by: xRaf1(S259A) [substitution]
    *** source: 11782426-Fig-3a

=====================================================
Raf1 phosphorylation on S259 itpo Hras{GTP} is unclear
=====================================================

  *** xRaf1[tAb]IP phos(S259)[phosAb] is decreased itpo xHras(G12V)"CA"
    *** cells: COS1 in BMS
    *** source: 11782426-Fig-5
  *** xRaf1[phosAb] phos(S259)[phosAb] is increased itpo xHras(G12V)"CA"
    *** cells: COS1 in BMS
    *** unaffected by: xRaf1(R89L)"Ras-binding-mut" [substitution]
    *** unaffected by: xRaf1(C168S)"Zinc-Finger-mut" [substitution]
    *** comment: the xRaf1 is a FRET probe with XFPs at both ends
    *** source: 15711535-Fig-1d

==========================================================
Raf1 activity itpo Hras{GTP} requires Raf1 Ras-binding-site
==========================================================

  *** xRaf1[tAb]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased itpo xHras(G12R)"CA"
    *** cells: COS in BMS
    *** inhibited by: xRaf1(R89L)"Ras-binding-mut" [substitution]
    *** source: 10205168-Fig-3a
  *** xRaf1[tAb]IP IVKA(Mek?,Erk?,MBP)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** inhibited by: xRaf1(R89L)"Ras-binding-mut" [substitution]
    *** source: 11259591-Fig-8e
  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,MBP)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS1 in BMS
    *** inhibited by: xRaf1(S259A/R89L)"Ras-binding-mut" [substitution]
    *** source: 11782426-Fig-3a
  *** xRaf1[tAb]IP phos(S338)[phosAb] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** inhibited by: xRaf1(R89L)"Ras-binding-mut" [substitution]
    *** source: 11259591-Fig-8a
  *** xRaf1[tAb]IP phos(S338)[phosAb] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** inhibited by: xRaf1(S257L/S89L) [substitution]
    *** source: 12077328-Fig-5c

==================================================
Effect of Cdc42{GTP} on Raf1 activity itpo Hras{GTP}
==================================================

  *** xRaf1[tAb]IP IVKA(Mek?,Erk?,MBP)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** unaffected by: xCdc42(G12V)"CA" [addition]
    *** source: 11259591-Fig-7a,7b
  *** xRaf1[tAb]IP phos(S338)[phosAb] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** unaffected by: xCdc42(G12V)"CA" [addition]
    *** unaffected by: xCdc42(T17N)"DN" [addition]
    *** source: 11259591-Fig-6a

=============================================
Raf1 relocates to cell membrane itpo Hras{GTP}
=============================================

  *** xRaf1[tAb]IP infraction(membrane)[WB] is increased itpo xHras(Q61L)"CA"
    *** cells: CHO in BMS
    *** source: 8120027-Fig-6a
  *** xRaf1[tAb] infraction(membrane)[WB] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** source: 9727023-Fig-2a
  *** xRaf1[tAb] locatedin(cellmembrane)[IHC] is increased itpo xHras(G12V)"CA"
    *** cells: MDCK in BMS
    *** source: 9020159-Fig-1
  *** xRaf1[tAb] infraction(membrane)[WB] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** inhibited by: xRaf1(R89L)"Ras-binding-mut" [substitution]
    *** source: 11259591-Fig-8d,10b
  *** xRaf1[tAb] infraction(membrane)[WB] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** unaffected by: xPak3(mnr)"KD" [addition]
    *** source: 11259591-Fig-10b


==============================
Raf1-Hras physical interaction
==============================

  *** rRaf1[tAb] boundby[WB] rHras{GTPgS}[GST] is detectable
    *** cells: none
    *** inhibited by: rHras{GDPbS} [substitution]
    *** source: 9674433-Fig-5b

  *** xRaf1[Ab] snaggedby[WB] rHras(G12V)[GST] is detectable
    *** cells: NIH3T3 in BMLS
    *** unaffected by: xRaf1(S29A/S289A/S296A/S301A/S43A/S642A) [substitution]
    *** source: 15664191-Fig-4c

  *** Raf1[Ab] copptby[WB] xHras[GST] is detectable
    *** cells: HEK293T in BMS
    *** source: 16630891-Fig-1b

  *** xRaf1[tAb] copptby[WB] xHras(G12R)[tAb] is detectable
    *** cells: NIH3T3 in BMS
    *** unaffected by: xSrc(Y527F) [addition]
    *** source: 10064593-Fig-4c
  *** xRaf1[tAb] copptby[WB] xHras(G12V)[tAb] is detectable
    *** cells: COS in BMS
    *** unaffected by: xPak3(mnr)"KD" [addition]
    *** source: 11259591-Fig-10a
  *** xRaf1[tAb] copptby[WB] xHras(G12V)[tAb] is detectable
    *** cells: COS1 in BMS
    *** enhanced by: xRaf1(S259A) [substitution]
    *** source: 11782426-Fig-3a
  *** xRaf1[tAb] copptby[WB] xHras(G12V)[tAb] is detectable
    *** cells: COS7 in BMS
    *** unaffected by: xRaf1(S471A) [substitution]
    *** unaffected by: xRaf1(S481A) [substitution]
    *** source: 16093354-Fig-10a
  *** Raf1[Ab] copptby[WB] xHras(G12V)[Ab] is detectable
    *** cells: COS7 in BMS
    *** source: 16364920-Fig-2b
  *** Raf1[Ab] copptby[WB] xHras(G12V)[GST] is detectable
    *** cells: HEK293T in BMS
    *** source: 16630891-Fig-1b
  *** xRaf1[tAb] copptby[WB] xHras(G12V)[tAb] is detectable
    *** cells: COS in BMS
    *** inhibited by: xHras(G12V/E37G) [substitution]
    *** unaffected by: xRaf1(S257L) [substitution]
    *** unaffected by: xRaf1(S259A) [substitution]
    *** source: 12077328-Fig-6a
  *** xRaf1[tAb] copptby[WB] xHras(Q61L)"CA"[tAb] is detectable
    *** cells: COS in BMS
    *** source: 15632082-Fig-3a
  *** xRaf1[tAb] copptby[WB] xHras(Q61L)"CA"[tAb] is detectable
    *** cells: COS in BMS
    *** source: 15632082-Fig-3b
  *** xHras(Q61L)[tAb] copptby[WB] xRaf1[tAb] is detectable
    *** cells: COS<xRaf1><xHras(Q61L)> in BMS
    *** source: 16157584-Fig-5b
  *** xHras(G12V)[Ab] copptby[WB] xRaf1[tAb] is detectable
    *** cells: COS1 in BMS
    *** source: 11782426-Fig-5
  *** Raf1[Ab] copptby[WB] xHras(Q61L)[GST] is detectable
    *** cells: COS<xHras(Q61L)> in BMS
    *** source: 16157584-Fig-5c
  *** xRaf1[tAb] copptby[WB] xHras(Q61L)[GST] is detectable
    *** cells: COS<xRaf1><xHras(Q61L)> in BMS
    *** source: 16157584-Fig-5a

==================================================
Effect of Rap1a{GTP} on Raf1 activity itpo Hras{GTP}
==================================================

  *** xRaf1[tAb]IP IVKA(Mek1)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS7 in BMLS
    *** partially inhibited by: xRap1a(G12V)"CA" [addition]
    *** source: 9094716-Fig-5c
  *** xRaf1[tAb]IP IVKA(Erk2)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS7 in BMS
    *** inhibited by: xRap1a(G12V)"CA" [addition]
    *** inhibited by: xRap1a(G12V/S180A) [addition]
    *** inhibited by: xRap1a(G12V/S180E) [addition]
    *** source: 9867809-Fig-3a

  *** Hras[Ab] copptby[WB] Raf1[Ab] is decreased itpo xRap1a
    *** cells: CHO<xInsR> in BMS
    *** source: 9564038-Fig-3a

=============================================
Effect of Pak3 on Raf1 activity itpo Hras{GTP}
=============================================

  *** xRaf1[tAb]IP IVKA(Mek?,Erk?,MBP)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** unaffected by: xPak3(mnr)"CA" [addition]
    *** unaffected by: xPak3(mnr)"KD" [addition]
    *** source: 11259591-Fig-7a,7b
  *** xRaf1[Ab]IP IVKA(snr)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS7 in BMS
    *** partially inhibited by: xPak3(mnr)"DN" [addition]
    *** source: 9823899-Fig-4a

==================================================
Raf1 - Pi3k interaction is increased itpo Hras{GTP}
==================================================

  *** xRaf1[tAb]IP IVLKA(PtdIns)[TLC] is increased itpo xHras(G12V)"CA"
    *** cells: COS in BMS
    *** source: 9727023-Fig-4a,4b

  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,Elk1)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: HEK293 in BMS
    *** source: 11447113-Fig-7b
