=======================
Vav1-Yphos is a Rac1Gef
=======================

  *** rRac1 GDP-dissociation[3H-GDP] is unchanged by rVav1
    *** cells: none
    *** source: 8289830-Fig-2

  *** rRac1 GDP-dissociation[3H-GDP] is increased by rVav1{Yphos}
    *** cells: none
    *** comment: rVav1 was phosphorylated by rLck
    *** inhibited by: rVav1 [substitution]
    *** source: 8990121-Fig-1a

  *** rRac1{GDP} GTP-association[35S-GTPgS] is increased by rVav1{Yphos}
    *** cells: none
    *** comment: rVav1 was phosphorylated by rLck
    *** inhibited by: rVav1 [substitution]
    *** source: 8990121-Fig-1a
  *** rRac1{GDP} GTP-association[35S-GTPgS] is increased by rVav1{Yphos}
    *** cells: none
    *** comment: rVav1 was phosphorylated by rLck
    *** inhibited by: rVav1 [substitution]
    *** source: 8990121-Fig-2a
  *** rRac1{GDP} GTP-association[35S-GTPgS] is increased by rVav1{Yphos}
    *** cells: none
    *** comment: rVav1 was phosphorylated by rLck
    *** inhibited by: rVav1 [substitution]
    *** source: 8990121-Fig-2b
  *** rRac1{GDP} GTP-association[35S-GTPgS] is increased by xVav1{phos}[His]IP
    *** cells: none
    *** IPfrom: SF9 in BMS
    *** comment: Vav1-IP was phosphorylated by rLck
    *** inhibited by: xVav1[His]IP [substitution]
    *** source: 9822605-Fig-4a
  *** rRac1{GTP} GTP-association[35S-GTPgS] is increased by rVav1(Y174F){phos}
    *** cells: none
    *** comment: Vav1-IP was phosphorylated by rLck
    *** partially inhibited by: rVav1(Y174F) [substitution]
    *** source: 10669745-Fig-7c
  *** rRac1{GTP} GTP-association[35S-GTPgS] is increased by rVav1{phos}
    *** cells: none
    *** comment: Vav1-IP was phosphorylated by rLck
    *** inhibited by: rVav1 [substitution]
    *** source: 10669745-Fig-7b

  *** xVav1[tAb] snaggedby[WB] rRac1(G15N)"NucFree"[GST] is detectable
    *** cells: NIH3T3 in BMS
    *** source: 12376551-Fig-1

====================================
"oncogenic" Vav1 also requires Yphos
====================================

  *** rRac1{GDP} GTP-association[35S-GTPgS] is increased by rVav1(67-845){Yphos}
    *** cells: none
    *** comment: rVav1 was phosphorylated by rLck
    *** inhibited by: rVav1 [substitution]
    *** source: 8990121-Fig-2b

==========================
Yphos can be by Lck or Fyn
==========================

  *** xRac1[tAb]IP GTP-association[TLC] is increased itpo xVav1
    *** cells: COS7 in BMS
    *** comment: results are reported as bound labelled GDP
    *** enhanced by: xLck [addition]
    *** enhanced by: xLck(Y505F)"CA" [addition]
    *** enhanced by: xFyn [addition]
    *** enhanced by: xFyn(1-522)"CA" [addition]
    *** enhanced by: xVav1(67-845) [substitution]
    *** source: 8990121-Fig-4a

======================================
Rac1 binds GTP in the presence of Vav1
======================================

  *** Rac1[Ab] GTP-association[BDPD] is increased itpo xVav1
    *** cells: HEK293 in BMS
    *** source: 11448999-Fig-1b
  *** Rac1[Ab] GTP-association[BDPD] is increased itpo xVav1
    *** cells: HEK293 in BMS
    *** unaffected by: xVav2(L342R/L343S)"DH-domain-mut" [substitution]
    *** source: 11448999-Fig-6a

==================================================
Rac1 binds GTP in the presence of "oncogenic" Vav1
==================================================

  *** xRac1[tAb]IP GTP-association[TLC] is increased itpo xVav1(67-845)
    *** cells: COS7 in BMS
    *** comment: results are reported as bound labelled GDP
    *** inhibited by: xRac1(T17N) [substitution]
    *** source: 8990121-Fig-3a
  *** xRac1[tAb]IP GTP-association[TLC] is increased itpo xVav1(67-845)
    *** cells: COS7 in BMS
    *** comment: results are reported as bound labelled GDP
    *** inhibited by: xVav1(WT) [substitution]
    *** inhibited by: xVav1((67-845)/L213Q) [substitution]
    *** inhibited by: xVav1((67-845)/C464S) [substitution]
    *** source: 8990121-Fig-3b
  *** xRac1[tAb]IP GTP-association[TLC] is increased itpo xVav1(67-845)
    *** cells: COS7 in BMS
    *** comment: results are reported as bound labelled GDP
    *** source: 8990121-Fig-3a


